SRR1527259_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527259_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2932954
Sequences flagged as poor quality	0
Sequence length	52
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG	18279	0.6232283220261893	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	11946	0.40730267164094625	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10083	0.3437830937682623	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9772	0.33317944979703057	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	8800	0.300038800472152	No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8174	0.2786951312567466	No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6356	0.21670984270465882	No Hit
GGACATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	5653	0.19274083398512215	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5497	0.18742196434038855	No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	5204	0.17743203609739533	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5144	0.175386317003267	No Hit
GGACATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4968	0.16938554099382397	No Hit
GGACATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	4645	0.15837275320376656	No Hit
GGACATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	4192	0.14292757404309786	No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3804	0.12969859056773478	No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3562	0.1214475235547506	No Hit
GGACATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	3484	0.11878808873238379	No Hit
GGACATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	3441	0.11732199004825851	No Hit
GGACATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	3425	0.11677646495649097	No Hit
GGACATGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	3347	0.11411703013412416	No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3268	0.11142349999352189	No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3195	0.10893454176233244	No Hit
GGACATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3148	0.10733206180526526	No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3126	0.10658196480408488	No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3052	0.10405891125465998	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAC	35	1.02028935E-7	46.000004	11
TTAATCG	25	3.418462E-5	46.0	34
CGCATAT	20	6.313064E-4	46.0	39
CGAATTA	25	3.418462E-5	46.0	34
CGTGTAA	45	3.110472E-10	46.0	31
GCGTAAA	20	6.313064E-4	46.0	23
ATGGGGC	36830	0.0	43.539505	5
ATGGGCT	10510	0.0	43.48335	5
ATGGGGT	24840	0.0	43.37963	5
CATGGGG	176640	0.0	43.295574	4
TGGGATT	6945	0.0	43.284374	6
ATGGGAG	29465	0.0	43.283558	5
ATGGGGG	66495	0.0	43.225956	5
ATGGGAT	17525	0.0	43.15207	5
ACATGGG	302115	0.0	42.881714	3
GGACATG	313625	0.0	42.834084	1
CGTATAG	145	0.0	42.827587	31
TGGGGGG	29380	0.0	42.75119	6
GACATGG	312355	0.0	42.71591	2
ATGGGAC	12565	0.0	42.503784	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content