##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527259_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2932954
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.388760273771766	33.0	31.0	34.0	31.0	34.0
2	32.82473642614238	34.0	31.0	34.0	31.0	34.0
3	32.34847529146383	34.0	31.0	34.0	31.0	34.0
4	36.30511763907651	37.0	37.0	37.0	35.0	37.0
5	36.02110875247276	37.0	35.0	37.0	35.0	37.0
6	36.227984823491944	37.0	37.0	37.0	35.0	37.0
7	36.527189311526875	37.0	37.0	37.0	35.0	37.0
8	36.63013091920296	37.0	37.0	37.0	35.0	37.0
9	38.60550318893512	39.0	39.0	39.0	38.0	39.0
10	38.05928255267556	39.0	38.0	39.0	35.0	39.0
11	37.742516589077084	39.0	38.0	39.0	35.0	39.0
12	37.28601641894145	39.0	37.0	39.0	35.0	39.0
13	37.12346630734747	39.0	37.0	39.0	34.0	39.0
14	37.95951624198675	40.0	38.0	40.0	34.0	40.0
15	38.06772455347066	40.0	38.0	40.0	34.0	40.0
16	38.009397692565244	40.0	38.0	40.0	34.0	40.0
17	38.02450771474766	40.0	38.0	40.0	34.0	40.0
18	38.037754768741685	40.0	38.0	40.0	34.0	40.0
19	37.8245679952703	40.0	37.0	40.0	34.0	40.0
20	37.92083067105723	40.0	37.0	40.0	34.0	40.0
21	33.705580448926234	40.0	34.0	40.0	10.0	40.0
22	34.49871290173661	39.0	34.0	40.0	17.0	40.0
23	36.48377301519219	39.0	34.0	40.0	30.0	40.0
24	37.24425374554119	39.0	36.0	40.0	32.0	40.0
25	37.450771474765716	39.0	36.0	40.0	33.0	40.0
26	37.432886775585295	40.0	36.0	40.0	33.0	40.0
27	37.36344143140329	40.0	36.0	40.0	33.0	40.0
28	37.24557528007599	40.0	35.0	40.0	33.0	40.0
29	37.21266716082148	40.0	35.0	40.0	33.0	40.0
30	36.98125303022141	39.0	35.0	40.0	32.0	40.0
31	36.846792005602545	39.0	35.0	40.0	32.0	40.0
32	36.62112327707833	39.0	35.0	40.0	31.0	40.0
33	36.32144384126038	39.0	35.0	40.0	30.0	40.0
34	36.05803568688769	39.0	35.0	40.0	30.0	40.0
35	36.00502564990791	38.0	35.0	40.0	30.0	40.0
36	35.877590988471006	38.0	35.0	40.0	30.0	40.0
37	35.60606167024781	38.0	35.0	40.0	29.0	40.0
38	35.515582242339974	38.0	35.0	40.0	28.0	40.0
39	35.29067758989742	38.0	34.0	40.0	28.0	40.0
40	30.435294246005903	36.0	26.0	40.0	8.0	40.0
41	31.30445789466865	35.0	26.0	40.0	12.0	40.0
42	32.95448479587474	35.0	30.0	40.0	21.0	40.0
43	33.74153396200554	36.0	32.0	40.0	23.0	40.0
44	34.4201474008798	37.0	33.0	40.0	25.0	40.0
45	34.71067974472153	37.0	34.0	40.0	26.0	40.0
46	34.66938451813428	37.0	34.0	40.0	26.0	40.0
47	34.60611622275699	36.0	34.0	40.0	26.0	40.0
48	34.45749677628766	36.0	34.0	40.0	25.0	40.0
49	34.285075729111334	36.0	34.0	40.0	25.0	40.0
50	34.10688541313638	35.0	34.0	40.0	24.0	40.0
51	33.91867823361703	35.0	34.0	39.0	24.0	40.0
52	33.47467433856787	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	10.0
15	38.0
16	130.0
17	396.0
18	807.0
19	1513.0
20	2697.0
21	4439.0
22	7203.0
23	10784.0
24	15778.0
25	22580.0
26	31297.0
27	40857.0
28	50843.0
29	59985.0
30	67643.0
31	83641.0
32	110930.0
33	128022.0
34	231031.0
35	301597.0
36	364972.0
37	391269.0
38	678212.0
39	326279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.53255318699168	0.3672065773960314	0.04950640207790507	0.05073383353438206
8	99.15419744053266	0.6698366220540793	0.10194500152406072	0.0740209358892093
9	96.86841321070838	1.6261762032408282	0.6293313839903387	0.8760792020604483
10	58.016661700115314	28.378590322248492	5.511371811491077	8.093376166145122
11	35.25565010566139	27.797435622924876	18.245632219257445	18.70128205215629
12	31.74304813508838	18.392071611078798	25.31290978310604	24.551970470726783
13	24.391927046929478	18.53441956471189	27.20141536485059	29.87223802350804
14	24.151930101869993	22.194449691335084	29.37403723345133	24.279582973343597
15	28.326526771302923	18.642672200109516	27.67254447222834	25.358256556359223
16	29.65078893156865	19.985720880722983	26.49386932082808	23.869620866880283
17	33.81969168285626	20.825761331408536	21.309880755034	24.044666230701196
18	31.487606010868223	20.665786098247704	21.94715634817321	25.899451542710867
19	28.58070736874837	22.434037492575744	24.059395408178922	24.925859730496967
20	29.04505150779726	24.472630665192842	22.16570733806258	24.316610488947322
21	34.86089451113109	20.56997825400603	23.624373242812535	20.944753992050337
22	30.41012576399084	21.122629267284793	22.06325090676499	26.403994061959374
23	29.69115778835945	24.86257881985193	21.062553316553892	24.38371007523473
24	30.022257423744115	23.699144275702928	20.757502504301126	25.521095796251835
25	27.87169522604173	20.69858579439023	21.481755254259017	29.947963725309023
26	30.05843255639195	21.20254869322874	21.20554908123346	27.53346966914585
27	27.73490480928102	19.77777353480484	22.29694021795091	30.190381437963225
28	28.51630131260156	25.1034281478673	20.146002971747937	26.2342675677832
29	32.84732730209884	22.389133958459627	19.409203144679392	25.35433559476214
30	28.833115009645567	24.112890962490376	21.880806858886977	25.17318716897708
31	33.87540343285302	22.342627944386447	19.68343178924729	24.098536833513243
32	33.47488572954093	22.111223019522296	21.85857671139745	22.555314539539317
33	35.03805378468261	20.960540124393358	21.121538217101257	22.879867873822775
34	30.300031981408505	22.727052657491388	23.784007522791015	23.18890783830909
35	28.727249046524427	28.60276703964672	22.600286264291906	20.069697649536952
36	31.37100002250291	26.43447527646189	20.212284270397696	21.982240430637507
37	30.29924778908909	26.273920422891052	21.151746669057886	22.275085118961975
38	32.80123043184448	26.13453876194444	19.724107503902207	21.340123302308868
39	30.62874494451669	24.119062215091	19.89622067035487	25.355972170037443
40	29.307858220756277	28.037227996074947	20.20365815488412	22.45125562828466
41	29.08412474249511	23.938561600352408	20.773459113235326	26.203854543917153
42	28.035898278663762	22.660157643113394	21.453081091622987	27.850862986599857
43	26.707510584891548	22.777172775297533	23.808522056602317	26.706794583208605
44	26.474366798797387	21.25222557189782	23.654615790087398	28.61879183921739
45	27.41826158882819	24.298164921781932	22.35636835763534	25.927205131754537
46	27.840804867720394	23.155664903029503	23.955063734378378	25.048466494871725
47	27.928361644949085	22.348969673578242	23.276362329583076	26.446306351889596
48	29.98584362386863	21.987763872191653	23.517688992053746	24.50870351188597
49	28.95135757328618	22.780650497757552	23.779881989284522	24.488109939671745
50	25.724542560162895	23.32426625170391	24.1408832187617	26.810307969371493
51	25.756319396758354	24.56404021338214	23.39515041831546	26.284489971544044
52	28.16443762841149	22.985631550989208	23.204830351925057	25.645100468674244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	11.0
6	22.0
7	64.0
8	106.0
9	337.5
10	569.0
11	7571.5
12	14574.0
13	13662.5
14	12478.5
15	12206.0
16	10752.5
17	9299.0
18	8678.5
19	8058.0
20	8993.0
21	9928.0
22	9782.0
23	9636.0
24	10985.5
25	12335.0
26	14646.0
27	16957.0
28	18867.5
29	20778.0
30	21415.0
31	22052.0
32	24434.5
33	26817.0
34	28982.0
35	31147.0
36	33156.5
37	35166.0
38	38124.0
39	41188.0
40	41294.0
41	46357.5
42	51421.0
43	52536.5
44	53652.0
45	58259.0
46	62866.0
47	69990.0
48	77114.0
49	85969.5
50	94825.0
51	96150.5
52	97476.0
53	119785.0
54	142094.0
55	175098.0
56	208102.0
57	224239.0
58	240376.0
59	273208.0
60	306040.0
61	281871.0
62	257702.0
63	261462.0
64	247906.5
65	230591.0
66	196691.0
67	162791.0
68	138201.0
69	113611.0
70	101666.5
71	89722.0
72	73290.0
73	56858.0
74	55474.0
75	54090.0
76	40464.0
77	26838.0
78	18644.0
79	10450.0
80	7375.0
81	4300.0
82	2931.0
83	1562.0
84	892.0
85	222.0
86	221.5
87	221.0
88	117.5
89	11.5
90	9.0
91	7.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2932954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.280258247700429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55040142771247	9.277784402497446
2	9.172464854535596	2.252804743633061
3	3.8433555388798974	1.4159219566552497
4	2.064045669366641	1.013880554194802
5	1.1868839815991885	0.7287620902048481
6	0.7307974494740193	0.5384628843781057
7	0.50732045211998	0.4361018316461447
8	0.35146422903361374	0.34528571978893663
9	0.27365817084382904	0.30245337085999907
>10	2.6563833865505737	8.118390858954736
>50	1.7164220572872475	15.413329084627472
>100	1.8066407153295967	39.712412077724785
>500	0.0976301296137799	8.075915050192496
>1k	0.038987609515733886	8.613135523668554
>5k	0.0025776931911229016	2.160435873299903
>10k+	9.666349466710882E-4	1.594923977673489
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG	18279	0.6232283220261893	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	11946	0.40730267164094625	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10083	0.3437830937682623	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9772	0.33317944979703057	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	8800	0.300038800472152	No Hit
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8174	0.2786951312567466	No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6356	0.21670984270465882	No Hit
GGACATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	5653	0.19274083398512215	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5497	0.18742196434038855	No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	5204	0.17743203609739533	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5144	0.175386317003267	No Hit
GGACATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4968	0.16938554099382397	No Hit
GGACATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	4645	0.15837275320376656	No Hit
GGACATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	4192	0.14292757404309786	No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3804	0.12969859056773478	No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3562	0.1214475235547506	No Hit
GGACATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	3484	0.11878808873238379	No Hit
GGACATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	3441	0.11732199004825851	No Hit
GGACATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	3425	0.11677646495649097	No Hit
GGACATGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	3347	0.11411703013412416	No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3268	0.11142349999352189	No Hit
GGACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3195	0.10893454176233244	No Hit
GGACATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3148	0.10733206180526526	No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3126	0.10658196480408488	No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3052	0.10405891125465998	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.7504850059018996E-4	0.0	0.0	0.0	0.0
8	0.0017388612300090625	0.0	0.0	0.0	0.0
9	0.00487563050767247	0.0	0.0	0.0	0.0
10	0.031333597458398595	0.0	0.0	0.0	0.0
11	0.05881442395618888	0.0	0.0	0.0	0.0
12	0.08148781058277764	0.0	0.0	0.0	0.0
13	0.09966061520228411	0.0	0.0	0.0	0.0
14	0.13576755721364878	0.0	0.0	0.0	0.0
15	0.15837275320376656	0.0	0.0	0.0	0.0
16	0.18725148774921122	0.0	0.0	0.0	0.0
17	0.20365133582047315	0.0	0.0	0.0	0.0
18	0.2191988009358483	0.0	0.0	0.0	0.0
19	0.24026970760536986	0.0	0.0	0.0	0.0
20	0.2635909052784326	0.0	0.0	0.0	0.0
21	0.28033170653204925	0.0	0.0	0.0	0.0
22	0.3007207068368614	0.0	0.0	0.0	0.0
23	0.3258830516946396	0.0	0.0	0.0	0.0
24	0.35206825609948195	0.0	0.0	0.0	0.0
25	0.37460526145312883	0.0	0.0	0.0	0.0
26	0.397960554444427	0.0	0.0	0.0	0.0
27	0.4237707103486792	0.0	0.0	0.0	0.0
28	0.49110896386373604	0.0	0.0	0.0	0.0
29	0.5090431012555942	0.0	0.0	0.0	0.0
30	0.5360806886163233	0.0	0.0	0.0	0.0
31	0.5504689129116924	0.0	0.0	0.0	0.0
32	0.5643798027517649	0.0	0.0	0.0	0.0
33	0.5756653530877062	0.0	0.0	0.0	0.0
34	0.5926789168872065	0.0	0.0	0.0	0.0
35	0.6570849730340128	0.0	0.0	0.0	0.0
36	0.6731779632411555	0.0	0.0	0.0	0.0
37	0.6859978028976929	0.0	0.0	0.0	0.0
38	0.6976584017342242	0.0	0.0	0.0	0.0
39	0.7115692915742967	0.0	0.0	0.0	0.0
40	0.7268098988255527	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAC	35	1.02028935E-7	46.000004	11
TTAATCG	25	3.418462E-5	46.0	34
CGCATAT	20	6.313064E-4	46.0	39
CGAATTA	25	3.418462E-5	46.0	34
CGTGTAA	45	3.110472E-10	46.0	31
GCGTAAA	20	6.313064E-4	46.0	23
ATGGGGC	36830	0.0	43.539505	5
ATGGGCT	10510	0.0	43.48335	5
ATGGGGT	24840	0.0	43.37963	5
CATGGGG	176640	0.0	43.295574	4
TGGGATT	6945	0.0	43.284374	6
ATGGGAG	29465	0.0	43.283558	5
ATGGGGG	66495	0.0	43.225956	5
ATGGGAT	17525	0.0	43.15207	5
ACATGGG	302115	0.0	42.881714	3
GGACATG	313625	0.0	42.834084	1
CGTATAG	145	0.0	42.827587	31
TGGGGGG	29380	0.0	42.75119	6
GACATGG	312355	0.0	42.71591	2
ATGGGAC	12565	0.0	42.503784	5
>>END_MODULE