FastQCFastQC Report
Fri 17 Jun 2016
SRR1527258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789748
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC191991.072720852321109No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG107070.5982406461691814No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT71880.40162078683702956No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT71370.39877122365830275No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAATTGCT44120.24651515185378053No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT43140.24103952064759956No Hit
CGCACCGGGGGTCAGTGATGCTCGGAAGCTGGGCTGGCATGATTGCCAAC36060.20148087887233287No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG34210.1911442281259708No Hit
CGCACCGGGGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGG32410.18108694631869962No Hit
CGCACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG24850.13884636272816062No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGA24640.13767301318397898No Hit
CGCACCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG23790.13292374121943426No Hit
CGCACCGGAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGT23770.1328119936437979No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC22280.12448679925889009No Hit
CGCACCGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTC21320.11912291562834545No Hit
CGCACCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC20700.11565874078361871No Hit
CGCACCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATC20400.1139825271490735No Hit
CGCACCGGGATTTGCTATGCTAAAGAAACAGCCCTTCACCGGAAGGTTCT19720.11018310957743771No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGC19630.10968024548707415No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT19110.10677480852052915No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT18440.10303126473671155No Hit
CGCACCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA18210.10174616761689356No Hit
CGCACCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAATTGC18170.10152267246562087No Hit
CGCACCGGGAGCCCTGCTCGATGGCAAGAACGTCAATGCGGGTGGCCACA18040.1007963132239846No Hit
CGCACCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC18000.1005728180727119No Hit
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT17940.10023757534580288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG207.8576594E-444.019
GATACGC551.8189894E-1244.017
CGTATAG351.4466968E-744.031
CGAAACG254.443838E-544.038
TAGACCG1250.044.036
TAGCGTA454.802132E-1044.029
TAGCGGT408.3164196E-944.021
AAGCGTA408.3164196E-944.039
TACGGTC454.802132E-1044.021
CGATTAT207.8576594E-444.018
CGATTAC302.5285117E-644.030
TAACGCA454.802132E-1044.022
CTACGTT254.443838E-544.015
CGGATAG302.5285117E-644.06
CGTAGCG207.8576594E-444.012
CGTAAGT207.8576594E-444.041
CGTAAGG351.4466968E-744.019
CGTAACT302.5285117E-644.026
GTATACG408.3164196E-944.037
CGCACTA302.5285117E-644.038
Produced by FastQC (version 0.11.5)