FastQCFastQC Report
Fri 17 Jun 2016
SRR1527257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923605
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC183540.9541459915107311No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT105430.5480854957228746No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC67250.3496039987419455No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT63270.3289136803033887No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC43380.2255140738353248No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAATTGCTCT42920.2231227304982052No Hit
CGCACCGGGGGTCAGTGATGCTCGGAAGCTGGGCTGGCATGATTGCCAACTC34920.18153415072221168No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT34330.17846699296373217No Hit
CGCACCGGGGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGA30060.15626908850829563No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGAAG25290.13147189781685947No Hit
CGCACCGGAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTA24980.12986034035053975No Hit
CGCACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC24810.12897658303029988No Hit
CGCACCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC22140.11509639453006204No Hit
CGCACCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC21610.11234115111990246No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC20920.10875413611422304No Hit
CGCACCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT20390.10599889270406346No Hit
CGCACCGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG20210.10506314965910361No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC19780.10282776349614395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA206.312521E-446.042
AGCGTAA206.312521E-446.020
GTCGCGA301.8620613E-646.017
AGTTTCG551.8189894E-1246.010
CGTATAG453.110472E-1046.031
GGCGTAC206.312521E-446.030
CGTTAAC206.312521E-446.09
GTTACGC253.4180204E-546.036
TCGCTAC206.312521E-446.029
ACGTGCG206.312521E-446.022
ACTCGAA253.4180204E-546.025
CGTCGTT206.312521E-446.034
CGTAAGA253.4180204E-546.038
GCGTACT206.312521E-446.031
AATCGGT253.4180204E-546.019
ACCGGTA12250.045.624494
CCGGGAT251550.044.7930835
CGCACCG1964000.044.6614531
CGGGATA30150.044.6268656
ACCGGAT22350.044.5592844
Produced by FastQC (version 0.11.5)