##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527257_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1923605 Sequences flagged as poor quality 0 Sequence length 52 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49941438081103 34.0 31.0 34.0 31.0 34.0 2 32.666327026598495 34.0 31.0 34.0 31.0 34.0 3 32.87590851552164 34.0 31.0 34.0 31.0 34.0 4 36.31011564224464 37.0 37.0 37.0 35.0 37.0 5 36.33237696928423 37.0 37.0 37.0 35.0 37.0 6 36.34188255904928 37.0 37.0 37.0 35.0 37.0 7 36.49621361974002 37.0 37.0 37.0 35.0 37.0 8 36.60479516324817 37.0 37.0 37.0 35.0 37.0 9 38.614149994411534 39.0 39.0 39.0 38.0 39.0 10 38.03163435320661 39.0 38.0 39.0 37.0 39.0 11 37.850392362257324 39.0 38.0 39.0 35.0 39.0 12 37.455142297924986 39.0 37.0 39.0 35.0 39.0 13 37.41763615711126 39.0 37.0 39.0 35.0 39.0 14 38.24916082043871 40.0 38.0 40.0 35.0 40.0 15 38.11922562064457 40.0 38.0 40.0 35.0 40.0 16 32.95370463270786 40.0 35.0 40.0 3.0 40.0 17 34.52778610993421 40.0 34.0 40.0 16.0 40.0 18 36.54744867059505 40.0 34.0 40.0 30.0 40.0 19 37.46271349887321 40.0 37.0 40.0 33.0 40.0 20 37.80877207118925 40.0 37.0 40.0 33.0 40.0 21 38.01785969572755 40.0 38.0 40.0 34.0 40.0 22 38.06949971537816 40.0 38.0 40.0 34.0 40.0 23 38.09073016549655 40.0 38.0 40.0 34.0 40.0 24 38.06077235191216 40.0 38.0 40.0 34.0 40.0 25 37.96837500422384 40.0 37.0 40.0 34.0 40.0 26 37.863085716662205 40.0 37.0 40.0 34.0 40.0 27 37.7282706168886 40.0 37.0 40.0 34.0 40.0 28 37.63737149778671 40.0 37.0 40.0 34.0 40.0 29 37.411891734529696 40.0 36.0 40.0 33.0 40.0 30 37.401528380306765 40.0 36.0 40.0 33.0 40.0 31 37.34563228937334 40.0 35.0 40.0 33.0 40.0 32 37.17175979476036 39.0 35.0 40.0 33.0 40.0 33 36.781707783042776 39.0 35.0 40.0 32.0 40.0 34 36.63443794334076 39.0 35.0 40.0 31.0 40.0 35 36.63326202624759 39.0 35.0 40.0 31.0 40.0 36 36.6679593783547 39.0 35.0 40.0 32.0 40.0 37 36.3091570254808 38.0 35.0 40.0 31.0 40.0 38 36.27257467099535 38.0 35.0 40.0 31.0 40.0 39 35.8937484566738 38.0 35.0 40.0 30.0 40.0 40 35.86644919305159 38.0 35.0 40.0 30.0 40.0 41 35.81026458134596 38.0 35.0 40.0 30.0 40.0 42 35.70416587605044 38.0 35.0 40.0 30.0 40.0 43 35.526172473038905 37.0 35.0 40.0 30.0 40.0 44 35.75331214048622 37.0 35.0 40.0 31.0 40.0 45 35.70740562641499 37.0 35.0 40.0 31.0 40.0 46 35.57633973710819 37.0 35.0 40.0 30.0 40.0 47 35.254498714652954 37.0 35.0 40.0 29.0 40.0 48 35.18903257165582 36.0 35.0 40.0 29.0 40.0 49 35.1390015101853 36.0 35.0 40.0 29.0 40.0 50 34.63312894279231 36.0 34.0 40.0 27.0 40.0 51 34.69643611864182 35.0 34.0 39.0 28.0 40.0 52 34.37426186769113 35.0 34.0 39.0 28.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 0.0 14 2.0 15 18.0 16 53.0 17 98.0 18 205.0 19 374.0 20 799.0 21 1387.0 22 2519.0 23 3747.0 24 5398.0 25 7846.0 26 11062.0 27 15416.0 28 20992.0 29 27856.0 30 35062.0 31 43100.0 32 54305.0 33 81186.0 34 128237.0 35 174360.0 36 225627.0 37 369636.0 38 463514.0 39 250804.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.14862978626068 0.4657920934911274 0.05479295385487145 0.3307851663933084 8 99.12778351064797 0.5627974558186322 0.15211023053069628 0.15730880300269545 9 92.68670023211627 4.42804006019947 1.4600710644856922 1.4251886431985776 10 42.113635595665436 40.00462672950008 6.15557767836952 11.72615999646497 11 31.321555100969274 19.193337509519885 28.565635876388345 20.9194715131225 12 29.11008237138082 14.15909191336059 29.473150672825245 27.25767504243335 13 23.125381770165912 14.916212008182553 33.20468599322626 28.753720228425273 14 22.588005333735357 19.650344015533335 33.38559631525183 24.376054335479477 15 27.45813199695364 20.36774701666922 29.639764920552814 22.534356065824323 16 28.371521180283892 29.37177850962126 24.065439630277528 18.191260679817322 17 29.706930476891046 21.122631725328226 25.784607546767656 23.385830251013072 18 28.95214973968148 21.93704008879162 26.48828631657747 22.62252385494943 19 26.530758653673704 22.61290649587623 28.101039454565775 22.75529539588429 20 27.9630173554342 23.050730269468005 26.653548935462325 22.332703439635477 21 25.929855661635315 21.817109021862596 29.34500586139046 22.908029455111624 22 25.378183150906764 20.32329922203363 30.088869596408824 24.209648030650783 23 27.032005011423866 22.727119133086056 27.115754013947768 23.125121841542313 24 26.277484202837904 21.90621255403266 26.761159385632705 25.05514385749673 25 25.0014945895857 23.627511885236313 24.331450583669724 27.03954294150826 26 24.642117274596394 23.094658206856398 26.03907766927202 26.224146849275186 27 21.683609680781657 22.070123544074796 25.95246945188851 30.293797323255035 28 24.53798986798225 24.48023372781834 25.75549554092446 25.226280863274948 29 27.62292674431601 21.783734186592362 25.67075880963088 24.92258025946075 30 26.059819973435296 23.047247225911764 26.727108735941112 24.16582406471183 31 29.954278555108765 21.115717623940466 25.921018088432916 23.00898573251785 32 30.05996553346451 21.21303490061629 27.36232230629469 21.364677259624507 33 29.093602896644583 21.48679172699177 27.140811133262805 22.278794243100844 34 24.263713184359574 23.420244800777706 30.484169047179645 21.831872967683076 35 23.91759222917387 28.000914948755074 28.240725096888397 19.840767725182666 36 25.63873560320336 28.470242071527156 25.993486188692582 19.897536136576896 37 27.445239537223078 27.48136961590347 24.621530927607278 20.45185991926617 38 27.495613704476753 26.496136161010185 25.16072686440303 20.847523270110027 39 27.010222992766188 25.551191642774896 24.11300656839632 23.3255787960626 40 24.396068839496674 25.078381476446566 25.081760548553365 25.44378913550339 41 22.56949841573504 25.83820482895397 24.809147408121728 26.783149347189262 42 22.31398857873628 25.412805643570273 24.75425048281742 27.51895529487603 43 22.75477553863709 23.73881332186182 26.540739912819937 26.965671226681152 44 24.083530662480083 23.01964280608545 25.806441551150055 27.090384980284416 45 26.497175875504585 24.274266286477733 24.305249778410847 24.923308059606832 46 24.189841469532467 25.421227330974915 25.599382409590326 24.789548789902295 47 24.520886564549375 23.949095578354186 26.459278282183714 25.070739574912732 48 24.970199183304263 24.056965957148165 26.998006347456986 23.97482851209058 49 25.239589208803263 23.814504537054123 27.893824355831885 23.052081898310725 50 23.574902331819683 23.64362745989951 27.41602355993044 25.365446648350364 51 22.277702542881723 23.60209086584824 28.254553299663915 25.86565329160613 52 22.63474050025863 23.623820898781194 28.07988126460474 25.66155733635544 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 26.0 10 52.0 11 51.5 12 51.0 13 311.5 14 1103.5 15 1635.0 16 4211.5 17 6788.0 18 7626.5 19 8465.0 20 7469.0 21 6473.0 22 7740.5 23 9008.0 24 9328.0 25 9648.0 26 9328.0 27 9008.0 28 9769.5 29 10531.0 30 11882.0 31 13233.0 32 15934.0 33 18635.0 34 20727.0 35 22819.0 36 25982.5 37 29146.0 38 31346.5 39 37282.5 40 41018.0 41 44120.0 42 47222.0 43 51160.5 44 55099.0 45 61309.5 46 67520.0 47 70918.5 48 74317.0 49 75775.0 50 77233.0 51 85466.0 52 93699.0 53 93136.0 54 92573.0 55 95482.5 56 98392.0 57 101448.0 58 104504.0 59 115625.0 60 126746.0 61 138070.5 62 149395.0 63 163504.0 64 166363.5 65 155114.0 66 143347.0 67 131580.0 68 110189.5 69 88799.0 70 77631.5 71 66464.0 72 49183.5 73 31903.0 74 29534.0 75 27165.0 76 21090.5 77 15016.0 78 13290.0 79 11564.0 80 9624.5 81 7685.0 82 4769.5 83 1854.0 84 1475.5 85 1097.0 86 674.5 87 252.0 88 177.0 89 63.5 90 25.0 91 18.5 92 12.0 93 12.5 94 13.0 95 10.0 96 7.0 97 9.0 98 11.0 99 5.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1923605.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.59913951641576 #Duplication Level Percentage of deduplicated Percentage of total 1 71.34990608476252 6.848977029907973 2 9.906137467978501 1.9018079124783835 3 4.187992785249722 1.2060338111806412 4 2.130812714451924 0.8181587411750637 5 1.2318502325095253 0.5912351122594063 6 0.7411590109947283 0.4268693250232271 7 0.5014831878912109 0.33696649599932704 8 0.374517898223418 0.287603964515311 9 0.2718669025885675 0.23487194950591253 >10 3.9488594450399677 10.19617668223103 >50 2.7590756423934772 19.22290967466003 >100 2.44167635079891 41.82038935216247 >500 0.11366745667672362 7.4921678754933385 >1k 0.038510285868616746 6.114686502023106 >5k 0.0012422672860844112 0.778205725946132 >10k+ 0.0012422672860844112 1.722939845438659 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 18354 0.9541459915107311 No Hit CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 10543 0.5480854957228746 No Hit CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 6725 0.3496039987419455 No Hit CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 6327 0.3289136803033887 No Hit CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC 4338 0.2255140738353248 No Hit CGCACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAATTGCTCT 4292 0.2231227304982052 No Hit CGCACCGGGGGTCAGTGATGCTCGGAAGCTGGGCTGGCATGATTGCCAACTC 3492 0.18153415072221168 No Hit CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT 3433 0.17846699296373217 No Hit CGCACCGGGGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGA 3006 0.15626908850829563 No Hit CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGAAG 2529 0.13147189781685947 No Hit CGCACCGGAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTA 2498 0.12986034035053975 No Hit CGCACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC 2481 0.12897658303029988 No Hit CGCACCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC 2214 0.11509639453006204 No Hit CGCACCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 2161 0.11234115111990246 No Hit CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 2092 0.10875413611422304 No Hit CGCACCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT 2039 0.10599889270406346 No Hit CGCACCGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG 2021 0.10506314965910361 No Hit CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC 1978 0.10282776349614395 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.198572471999189E-5 0.0 0.0 0.0 0.0 8 2.5992862359995947E-4 0.0 0.0 0.0 0.0 9 5.198572471999189E-4 0.0 0.0 0.0 0.0 10 0.004782686674239254 0.0 0.0 0.0 0.0 11 0.00904551610127859 0.0 0.0 0.0 0.0 12 0.0134643027024779 0.0 0.0 0.0 0.0 13 0.020482375539676804 0.0 0.0 0.0 0.0 14 0.03628603585455434 0.0 0.0 0.0 0.0 15 0.04897055268623236 0.0 0.0 0.0 0.0 16 0.06903704242814923 0.0 0.0 0.0 0.0 17 0.07730277265862794 0.0 0.0 0.0 0.0 18 0.08795984622622628 0.0 0.0 0.0 0.0 19 0.1018920204511841 0.0 0.0 0.0 0.0 20 0.11083356510302271 0.0 0.0 0.0 0.0 21 0.12195851019310097 0.0 0.0 0.0 0.0 22 0.1320437407887794 0.0 0.0 0.0 0.0 23 0.1416091141372579 0.0 0.0 0.0 0.0 24 0.15398171662061597 0.0 0.0 0.0 0.0 25 0.16245538974997464 0.0 0.0 0.0 0.0 26 0.17014927700853347 0.0 0.0 0.0 0.0 27 0.1819500365199716 0.0 0.0 0.0 0.0 28 0.20872268475076744 0.0 0.0 0.0 0.0 29 0.21636458628460625 0.0 0.0 0.0 0.0 30 0.23071264630732402 0.0 0.0 0.0 0.0 31 0.23955021950972263 0.0 0.0 0.0 0.0 32 0.2474520496671614 0.0 0.0 0.0 0.0 33 0.2558217513470801 0.0 0.0 0.0 0.0 34 0.2717813688361176 5.198572471999189E-5 0.0 0.0 0.0 35 0.34346968322498644 5.198572471999189E-5 0.0 0.0 0.0 36 0.36010511513538385 5.198572471999189E-5 0.0 0.0 0.0 37 0.3713340316749021 5.198572471999189E-5 0.0 0.0 0.0 38 0.3803275620514607 5.198572471999189E-5 0.0 0.0 0.0 39 0.3877095349616995 5.198572471999189E-5 0.0 0.0 0.0 40 0.3967030653382581 5.198572471999189E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCGTA 20 6.312521E-4 46.0 42 AGCGTAA 20 6.312521E-4 46.0 20 GTCGCGA 30 1.8620613E-6 46.0 17 AGTTTCG 55 1.8189894E-12 46.0 10 CGTATAG 45 3.110472E-10 46.0 31 GGCGTAC 20 6.312521E-4 46.0 30 CGTTAAC 20 6.312521E-4 46.0 9 GTTACGC 25 3.4180204E-5 46.0 36 TCGCTAC 20 6.312521E-4 46.0 29 ACGTGCG 20 6.312521E-4 46.0 22 ACTCGAA 25 3.4180204E-5 46.0 25 CGTCGTT 20 6.312521E-4 46.0 34 CGTAAGA 25 3.4180204E-5 46.0 38 GCGTACT 20 6.312521E-4 46.0 31 AATCGGT 25 3.4180204E-5 46.0 19 ACCGGTA 1225 0.0 45.62449 4 CCGGGAT 25155 0.0 44.793083 5 CGCACCG 196400 0.0 44.661453 1 CGGGATA 3015 0.0 44.626865 6 ACCGGAT 2235 0.0 44.559284 4 >>END_MODULE