FastQCFastQC Report
Fri 17 Jun 2016
SRR1527256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856580
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC170570.9187322927102521No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT103400.5569380258324446No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC65900.3549537321311228No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT63520.34213446229087896No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAATTGCTCT41880.22557605920563617No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC40050.2157192256730117No Hit
CGCACCGGGGGTCAGTGATGCTCGGAAGCTGGGCTGGCATGATTGCCAACTC33410.1799545400682976No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT33150.17855411563196844No Hit
CGCACCGGGGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGA29730.16013314804640794No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGAAG25560.13767249458682093No Hit
CGCACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC24190.13029333505693264No Hit
CGCACCGGAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTA23060.12420687500673282No Hit
CGCACCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC22340.12032877656766744No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC21300.11472707882235077No Hit
CGCACCGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG19780.1065399821176572No Hit
CGCACCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT19530.10519342015964839No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC18970.10217712137370866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG301.8620267E-646.010
CGAAACG301.8620267E-646.038
CGATTAC206.312463E-446.030
CGTTAAT453.110472E-1046.035
CGCCTAC206.312463E-446.045
ACGATTT206.312463E-446.012
TTGCGTG405.6152203E-946.040
GCGATAA301.8620267E-646.08
TTACGCC301.8620267E-646.037
ACCGGTA11350.045.1894264
CCGGGAT242300.044.8704075
CGCACCG1894300.044.716621
GCACCGG1886950.044.60682
ACCGGGT87350.044.6044654
CGGGATT90000.044.5944486
ACCGGGA722150.044.5827064
CACCGGG1754250.044.576143
CACCGGT24850.044.519123
ACCGGGG750600.044.4311264
CGGGAGT57850.044.409686
Produced by FastQC (version 0.11.5)