##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527256_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1856580
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48254747977464	34.0	31.0	34.0	31.0	34.0
2	32.6810705706191	34.0	31.0	34.0	31.0	34.0
3	32.88221245515949	34.0	31.0	34.0	31.0	34.0
4	36.330360663154835	37.0	37.0	37.0	35.0	37.0
5	36.35591463874436	37.0	37.0	37.0	35.0	37.0
6	36.35495696387982	37.0	37.0	37.0	35.0	37.0
7	36.506282519471284	37.0	37.0	37.0	35.0	37.0
8	36.634403042152776	37.0	37.0	37.0	35.0	37.0
9	38.56147809413007	39.0	39.0	39.0	38.0	39.0
10	38.03427215633046	39.0	38.0	39.0	37.0	39.0
11	37.78599791013584	39.0	38.0	39.0	35.0	39.0
12	37.4310641071217	39.0	37.0	39.0	35.0	39.0
13	37.333355416949445	39.0	37.0	39.0	35.0	39.0
14	38.18761432311023	40.0	38.0	40.0	35.0	40.0
15	38.241949175365455	40.0	38.0	40.0	35.0	40.0
16	38.20337125251807	40.0	38.0	40.0	35.0	40.0
17	38.1371473354232	40.0	38.0	40.0	34.0	40.0
18	38.14775770502752	40.0	38.0	40.0	34.0	40.0
19	38.05202630643441	40.0	38.0	40.0	34.0	40.0
20	38.07133815941139	40.0	38.0	40.0	34.0	40.0
21	33.72674649085954	40.0	34.0	40.0	10.0	40.0
22	34.42669370563078	40.0	34.0	40.0	16.0	40.0
23	36.58224746577039	40.0	34.0	40.0	30.0	40.0
24	37.430122591000654	40.0	36.0	40.0	33.0	40.0
25	37.685782998847344	40.0	37.0	40.0	34.0	40.0
26	37.75993978174924	40.0	37.0	40.0	34.0	40.0
27	37.71385612254791	40.0	37.0	40.0	34.0	40.0
28	37.628506716651046	40.0	36.0	40.0	34.0	40.0
29	37.58950543472406	40.0	36.0	40.0	34.0	40.0
30	37.4890745348975	40.0	36.0	40.0	33.0	40.0
31	37.32624395393681	40.0	35.0	40.0	33.0	40.0
32	37.18567150351722	39.0	35.0	40.0	33.0	40.0
33	36.81588889247972	39.0	35.0	40.0	32.0	40.0
34	36.661247562722856	39.0	35.0	40.0	31.0	40.0
35	36.651783386657186	39.0	35.0	40.0	31.0	40.0
36	36.618314319878486	39.0	35.0	40.0	32.0	40.0
37	36.48107218649344	39.0	35.0	40.0	31.0	40.0
38	36.369738443805275	38.0	35.0	40.0	31.0	40.0
39	36.20869448125047	38.0	35.0	40.0	31.0	40.0
40	31.318059011731247	37.0	31.0	40.0	8.0	40.0
41	32.166155511747405	36.0	31.0	40.0	13.0	40.0
42	33.95595665147745	36.0	31.0	40.0	23.0	40.0
43	34.76535242219565	36.0	33.0	40.0	28.0	40.0
44	35.44934826401232	37.0	34.0	40.0	30.0	40.0
45	35.58843734177897	37.0	35.0	40.0	30.0	40.0
46	35.54518361718859	37.0	35.0	40.0	30.0	40.0
47	35.46605371166338	37.0	35.0	40.0	30.0	40.0
48	35.29797530943994	36.0	35.0	40.0	30.0	40.0
49	35.185355869394265	36.0	35.0	40.0	30.0	40.0
50	35.0592067134193	36.0	35.0	40.0	29.0	40.0
51	34.875981105042605	36.0	34.0	40.0	29.0	40.0
52	34.505285524997575	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	9.0
15	21.0
16	37.0
17	99.0
18	185.0
19	434.0
20	794.0
21	1513.0
22	2622.0
23	4037.0
24	5504.0
25	8033.0
26	11322.0
27	15946.0
28	21403.0
29	29274.0
30	36773.0
31	46626.0
32	65615.0
33	76156.0
34	144430.0
35	195995.0
36	233714.0
37	258037.0
38	443374.0
39	254625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.14337114479311	0.4765213457001583	0.05289295371058613	0.3272145557961413
8	99.15796787641793	0.554244901916427	0.13831884432666516	0.1494683773389781
9	92.68596020640102	4.41769274687867	1.500770233439981	1.3955768132803326
10	42.04338084003921	40.07255275829751	6.155888784754764	11.72817761690851
11	31.407965183294014	19.195025261502334	28.553900182055177	20.843109373148476
12	29.06613235088173	14.185168427969707	29.463152678581046	27.285546542567516
13	23.254640252507297	14.912635060164389	33.13248015167674	28.700244535651574
14	22.519255835999527	19.67887190425406	33.424091609303126	24.377780650443288
15	27.403074470262528	20.219381874198795	29.74582296480626	22.631720690732422
16	29.701548007626926	21.69650647965614	27.515808637387025	21.08613687532991
17	29.730256708571673	21.206250201984293	25.679367439054605	23.384125650389425
18	28.948065798403515	21.954346163375668	26.456172101390727	22.641415936830086
19	26.4852039772054	22.68655269366254	28.01392883689364	22.814314492238417
20	27.963351969750832	23.08820519449741	26.63241013045492	22.316032705296838
21	31.084467138501974	20.93494489868468	27.392140387163494	20.58844757564985
22	25.485570242057975	20.303030303030305	29.99218994064355	24.21920951426817
23	27.0632022320611	22.72457960335671	27.054422648094885	23.157795516487305
24	26.467537084316323	21.854431266091414	26.6966680670911	24.98136358250116
25	24.96504325157009	23.588533755615163	24.357636083551476	27.08878690926327
26	24.64396901830247	23.090359693630226	26.033513234010925	26.23215805405638
27	21.715681521938187	22.04009522886167	25.97033254694115	30.273890702258992
28	24.509528272414872	24.512167533852566	25.8344375141389	25.143866679593664
29	27.49814174449795	21.793728253024376	25.658630384901272	25.049499617576405
30	26.020424651779077	23.11292807204645	26.701300240226654	24.165347035947818
31	30.052785228753947	21.117215525320752	25.82792015426214	23.002079091663166
32	29.996660526344137	21.292214717383576	27.317756304603087	21.393368451669197
33	29.07841299593877	21.52646263559879	27.126059744260957	22.26906462420149
34	24.241616305249437	23.46680455461117	30.456969266069873	21.834609874069525
35	23.934654041301748	27.949670900257463	28.264874123388168	19.85080093505262
36	25.671611242176475	28.472729427226405	25.96451539928255	19.891143931314566
37	27.335638647405446	27.497764707149706	24.62861821198117	20.53797843346368
38	27.479236014607505	26.56341229572655	25.16810479483782	20.789246894828125
39	26.83067791315214	25.553867864568186	24.14741083066714	23.468043391612532
40	25.41506425793664	29.187215202145882	22.826595137295456	22.571125402622023
41	22.55970655721811	25.818601945512714	24.766721606394555	26.85496989087462
42	22.324812289263054	25.428853052386646	24.75277122450958	27.493563433840716
43	22.73562141141238	23.727283499768394	26.54536836548923	26.991726723329997
44	24.129205312994863	23.05130939684797	25.737108015814027	27.082377274343145
45	26.42870223744735	24.321817535468444	24.279427765030327	24.970052462053886
46	24.129582350343103	25.442587984358333	25.646295877365908	24.78153378793265
47	24.328711932693448	23.95027416001465	26.515151515151516	25.20586239214039
48	24.98443374376542	24.101519999138198	27.00799319178274	23.90605306531364
49	25.224875846987473	23.819065162826274	27.923763048185375	23.032295942000882
50	23.558424630234086	23.63081580109664	27.507783128117293	25.302976440551983
51	22.257591916319253	23.675521658102532	28.26358142390848	25.803305001669735
52	22.632259315515626	23.636471361320275	28.023624082991304	25.707645240172788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	30.5
10	61.0
11	64.5
12	68.0
13	299.0
14	983.0
15	1436.0
16	3848.5
17	6261.0
18	7125.0
19	7989.0
20	7120.0
21	6251.0
22	7501.0
23	8751.0
24	8964.0
25	9177.0
26	9071.0
27	8965.0
28	9568.0
29	10171.0
30	11458.0
31	12745.0
32	15520.5
33	18296.0
34	19879.0
35	21462.0
36	24109.5
37	26757.0
38	29498.5
39	35938.5
40	39637.0
41	42685.0
42	45733.0
43	49189.0
44	52645.0
45	58239.0
46	63833.0
47	67572.0
48	71311.0
49	73008.5
50	74706.0
51	82319.5
52	89933.0
53	89118.0
54	88303.0
55	91645.0
56	94987.0
57	97849.0
58	100711.0
59	110357.5
60	120004.0
61	132059.5
62	144115.0
63	157982.0
64	160585.0
65	149321.0
66	140092.0
67	130863.0
68	108774.0
69	86685.0
70	76124.0
71	65563.0
72	48557.5
73	31552.0
74	29146.0
75	26740.0
76	20775.5
77	14811.0
78	12971.0
79	11131.0
80	9358.5
81	7586.0
82	4755.5
83	1925.0
84	1515.0
85	1105.0
86	672.5
87	240.0
88	166.0
89	55.0
90	18.0
91	12.0
92	6.0
93	7.5
94	9.0
95	7.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1856580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.99407222517426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25191323421697	7.2209083926978845
2	9.575908289881406	1.9140463814143942
3	3.9137093551478865	1.1734168189106446
4	1.9901064835487803	0.7955707172949633
5	1.1526535726220488	0.5759851527694951
6	0.7326929854138582	0.439355196906279
7	0.4708946793887619	0.32943088053830943
8	0.3620117819495386	0.28943775161341795
9	0.2780238976779042	0.2500731822345689
>10	4.054841410031598	10.900195100456118
>50	2.7866252045650066	20.085508469783335
>100	2.283954621105462	40.41806900694488
>500	0.1096849776875565	7.444448009017308
>1k	0.03447242155894632	5.636720926748551
>5k	0.0012535426021435027	0.8106865760671633
>10k+	0.0012535426021435027	1.7161474366026945
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	17057	0.9187322927102521	No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	10340	0.5569380258324446	No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6590	0.3549537321311228	No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6352	0.34213446229087896	No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAATTGCTCT	4188	0.22557605920563617	No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	4005	0.2157192256730117	No Hit
CGCACCGGGGGTCAGTGATGCTCGGAAGCTGGGCTGGCATGATTGCCAACTC	3341	0.1799545400682976	No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3315	0.17855411563196844	No Hit
CGCACCGGGGAACTCTCATTTCCAAAAGCTATACAGAGAGGCACGTTTGGGA	2973	0.16013314804640794	No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGAAG	2556	0.13767249458682093	No Hit
CGCACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2419	0.13029333505693264	No Hit
CGCACCGGAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGTGGTTA	2306	0.12420687500673282	No Hit
CGCACCGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	2234	0.12032877656766744	No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2130	0.11472707882235077	No Hit
CGCACCGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG	1978	0.1065399821176572	No Hit
CGCACCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT	1953	0.10519342015964839	No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC	1897	0.10217712137370866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.3862478320352477E-5	0.0	0.0	0.0	0.0
8	3.7703734824246735E-4	0.0	0.0	0.0	0.0
9	7.002122181645822E-4	0.0	0.0	0.0	0.0
10	0.005170797918753837	0.0	0.0	0.0	0.0
11	0.00958752114102274	0.0	0.0	0.0	0.0
12	0.014435144189854464	0.0	0.0	0.0	0.0
13	0.020952504066617115	0.0	0.0	0.0	0.0
14	0.03560309816975299	0.0	0.0	0.0	0.0
15	0.04939189261976322	0.0	0.0	0.0	0.0
16	0.06705878550883883	0.0	0.0	0.0	0.0
17	0.07422249512544571	0.0	0.0	0.0	0.0
18	0.08343297891822599	0.0	0.0	0.0	0.0
19	0.09619838628014953	0.0	0.0	0.0	0.0
20	0.10546273255125015	0.0	0.0	0.0	0.0
21	0.11435004147410831	0.0	0.0	0.0	0.0
22	0.12544571200810092	0.0	0.0	0.0	0.0
23	0.13557185793232718	0.0	0.0	0.0	0.0
24	0.15173060142843292	0.0	0.0	0.0	0.0
25	0.16024087300304862	0.0	0.0	0.0	0.0
26	0.16837410722942184	0.0	0.0	0.0	0.0
27	0.18146268946126748	0.0	0.0	0.0	0.0
28	0.20957890314449149	0.0	0.0	0.0	0.0
29	0.21706578763102047	0.0	0.0	0.0	0.0
30	0.23203955660407846	0.0	0.0	0.0	0.0
31	0.23898781630740393	0.0	0.0	0.0	0.0
32	0.24652856327225328	0.0	0.0	0.0	0.0
33	0.2530459231490159	0.0	0.0	0.0	0.0
34	0.26591905546758016	0.0	0.0	0.0	0.0
35	0.32818408040590763	0.0	0.0	0.0	0.0
36	0.3437503366404895	0.0	0.0	0.0	0.0
37	0.35387648256471577	0.0	0.0	0.0	0.0
38	0.36400262848894205	0.0	0.0	0.0	0.0
39	0.37143565049715066	0.0	0.0	0.0	0.0
40	0.3811847590731345	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	30	1.8620267E-6	46.0	10
CGAAACG	30	1.8620267E-6	46.0	38
CGATTAC	20	6.312463E-4	46.0	30
CGTTAAT	45	3.110472E-10	46.0	35
CGCCTAC	20	6.312463E-4	46.0	45
ACGATTT	20	6.312463E-4	46.0	12
TTGCGTG	40	5.6152203E-9	46.0	40
GCGATAA	30	1.8620267E-6	46.0	8
TTACGCC	30	1.8620267E-6	46.0	37
ACCGGTA	1135	0.0	45.189426	4
CCGGGAT	24230	0.0	44.870407	5
CGCACCG	189430	0.0	44.71662	1
GCACCGG	188695	0.0	44.6068	2
ACCGGGT	8735	0.0	44.604465	4
CGGGATT	9000	0.0	44.594448	6
ACCGGGA	72215	0.0	44.582706	4
CACCGGG	175425	0.0	44.57614	3
CACCGGT	2485	0.0	44.51912	3
ACCGGGG	75060	0.0	44.431126	4
CGGGAGT	5785	0.0	44.40968	6
>>END_MODULE