FastQCFastQC Report
Fri 17 Jun 2016
SRR1527255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360826
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC103970.7640212635561049No Hit
CGCTTGGGGGTGTGTTAGAGTGCTCATTTTTCTTTTGCTCTAGCGTAGAT35220.2588133971573147No Hit
CGCTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC30300.22265888511830315No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA24820.18238922536753413No Hit
CGCTTGGGGGAGCACGGCCCTGCTGAAGCAACACAGGTTTCCAACCTCTT23490.17261574955211026No Hit
CGCTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA23310.1712930235019025No Hit
CGCTTGGGGACATAAAAGAATACATACTGGAGAGAAACCCTACAAATGTG22320.16401803022575995No Hit
CGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20650.1517460718710548No Hit
CGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA19390.1424869895196006No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG19030.13984153741918512No Hit
CGCTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG19030.13984153741918512No Hit
CGCTTGGGGAAGAGATGTGAAGAGAAACTCTCTACTCCTTATGTTGGCGC18670.13719608531876962No Hit
CGCTTGGGGAATACTTGTGTTTTAAAAGTAATTTTTATTCTTTGGCGGTT17710.13014154638432834No Hit
CGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG17630.12955366813979158No Hit
CGCTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT15880.11669383154054964No Hit
CGCTTGGGGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGC15840.11639989241828126No Hit
CGCTTGGGACAACAAATAGATGCTATATTGCTTGATTAGCTTGGTGCAGT14680.10787565787249802No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC14520.10669990138342449No Hit
CGCTTGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA14510.10662641660285739No Hit
CGCTTGGGGGCGTAGCACAGTGCACGGTGCCCTCAAGTTTGCCTTCGACT14090.10354005581903931No Hit
CGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT13960.10258475367166706No Hit
CGCTTGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC13810.10148248196316062No Hit
CGCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT13710.10074763415748964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA551.8189894E-1244.00000443
ATTATCG1100.044.00000411
ATAGTCG551.8189894E-1244.00000422
TATCGCG1300.044.00000413
AACGTCG207.8569935E-444.019
AACCGGT302.5280624E-644.016
GTACCGG950.044.022
ACACGCG207.8569935E-444.010
CGTATTA207.8569935E-444.031
TAGACGA302.5280624E-644.027
CACGCTA800.044.028
ACCGATA254.4432738E-544.044
TTCACGC207.8569935E-444.012
ATAGCGG207.8569935E-444.012
CGCGATA254.4432738E-544.029
TAACGGG207.8569935E-444.011
TAACGGC351.4463512E-744.027
AATGCGT254.4432738E-544.014
CTTATCG254.4432738E-544.038
CGCTAAT254.4432738E-544.012
Produced by FastQC (version 0.11.5)