##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1360826
Sequences flagged as poor quality	0
Sequence length	50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5255396354861	34.0	31.0	34.0	31.0	34.0
2	32.73674885694424	34.0	31.0	34.0	31.0	34.0
3	32.950014917410456	34.0	31.0	34.0	31.0	34.0
4	36.404378664134875	37.0	37.0	37.0	35.0	37.0
5	36.48895597232857	37.0	37.0	37.0	35.0	37.0
6	36.2128104548267	37.0	37.0	37.0	35.0	37.0
7	36.611140586673095	37.0	37.0	37.0	35.0	37.0
8	36.67812490355123	37.0	37.0	37.0	35.0	37.0
9	38.649579740539934	39.0	39.0	39.0	38.0	39.0
10	38.11577894602249	39.0	39.0	39.0	37.0	39.0
11	37.14277064077259	39.0	35.0	39.0	35.0	39.0
12	36.886419718612075	39.0	35.0	39.0	33.0	39.0
13	36.79126501110355	39.0	35.0	39.0	33.0	39.0
14	37.51965497425828	40.0	36.0	40.0	33.0	40.0
15	37.60847970276876	40.0	36.0	40.0	33.0	40.0
16	37.57930918427485	40.0	36.0	40.0	33.0	40.0
17	37.676910934976256	40.0	36.0	40.0	33.0	40.0
18	37.659685367563526	40.0	36.0	40.0	33.0	40.0
19	37.643945662413856	40.0	36.0	40.0	34.0	40.0
20	37.36053323496171	40.0	35.0	40.0	33.0	40.0
21	37.487493625195285	40.0	35.0	40.0	33.0	40.0
22	37.35242565912174	40.0	35.0	40.0	33.0	40.0
23	37.426141181899816	40.0	35.0	40.0	33.0	40.0
24	37.419936127028734	40.0	35.0	40.0	33.0	40.0
25	37.36546553343337	40.0	35.0	40.0	33.0	40.0
26	37.25950121470342	39.0	35.0	40.0	33.0	40.0
27	37.16260418304765	39.0	35.0	40.0	33.0	40.0
28	37.03551592929588	39.0	35.0	40.0	33.0	40.0
29	37.259668760003116	40.0	35.0	40.0	33.0	40.0
30	37.27127421139808	40.0	35.0	40.0	33.0	40.0
31	37.24893924719252	40.0	35.0	40.0	33.0	40.0
32	36.55348295814454	40.0	35.0	40.0	31.0	40.0
33	36.76967812196416	39.0	35.0	40.0	32.0	40.0
34	36.76411018014059	39.0	35.0	40.0	32.0	40.0
35	36.778561697086914	39.0	35.0	40.0	32.0	40.0
36	36.56238784385366	39.0	35.0	40.0	31.0	40.0
37	36.45639045697246	39.0	35.0	40.0	31.0	40.0
38	36.21187793296131	39.0	35.0	40.0	31.0	40.0
39	36.17461894467037	39.0	35.0	40.0	31.0	40.0
40	36.09324998199623	39.0	35.0	40.0	30.0	40.0
41	35.934685257336355	38.0	35.0	40.0	30.0	40.0
42	35.86792212964773	38.0	35.0	40.0	30.0	40.0
43	35.82353585248959	38.0	35.0	40.0	30.0	40.0
44	35.64537126715686	38.0	35.0	40.0	30.0	40.0
45	35.547870190604826	37.0	35.0	40.0	29.0	40.0
46	35.31799656973044	37.0	35.0	40.0	29.0	40.0
47	35.22108116687953	37.0	34.0	40.0	29.0	40.0
48	35.086310079319475	36.0	34.0	40.0	28.0	40.0
49	34.837628028858944	36.0	34.0	40.0	27.0	40.0
50	34.443580590024	35.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	5.0
15	24.0
16	79.0
17	145.0
18	305.0
19	516.0
20	913.0
21	1429.0
22	2175.0
23	3312.0
24	4403.0
25	6295.0
26	8347.0
27	11023.0
28	14731.0
29	18722.0
30	24614.0
31	30449.0
32	37726.0
33	53097.0
34	108074.0
35	120858.0
36	112667.0
37	186260.0
38	388632.0
39	226022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.32452789702725	0.5846449141918217	0.06981054153874192	0.02101664724218967
8	99.16080380592376	0.6188888219360889	0.08083325862380641	0.1394741135163496
9	93.11873817813593	3.5817951744014294	1.8932618865306807	1.4062047609319634
10	48.60834522562032	35.013072942462884	7.111122215477953	9.267459616438838
11	32.779062128442575	24.295317696751827	24.697793839917814	18.22782633488778
12	30.222453127732717	16.753574667150687	28.340360927848234	24.683611277268362
13	24.586758336480933	16.99460474741076	30.945028975048977	27.473607941059324
14	24.162898122169917	20.17098438742352	30.951716090080584	24.71440140032598
15	25.160454018368256	20.005202722464148	28.78134309603138	26.053000163136215
16	29.247310089607343	20.76768080562835	25.572777122130237	24.41223198263408
17	28.741734799305718	21.229385681931415	24.546121252827326	25.48275826593554
18	27.809653842592663	19.431874464479662	26.06916681486097	26.689304878066704
19	28.802286258493005	21.876786598727538	23.504107064385895	25.816820078393565
20	27.168866556047576	22.547335221402296	25.4636522229881	24.82014599956203
21	29.454316716464852	19.015950606469893	27.571122244871866	23.95861043219339
22	29.081087515964573	19.247868573939652	25.98877446492057	25.68226944517521
23	26.534472445411833	20.63643698753551	26.397643783995896	26.43144678305676
24	24.746293795092097	23.289531505129972	25.998694910297125	25.9654797894808
25	27.635715366990343	23.19466265341785	24.307369200764832	24.862252778826978
26	27.047175759428466	23.614040296114272	23.57384412114407	25.764939823313192
27	26.408666501080962	20.690301331691195	25.66779294340349	27.233239223824352
28	27.953022649479063	20.49365605889364	24.3308843305463	27.22243696108099
29	27.96015067319407	22.04859401569341	23.91584228990334	26.075413021209176
30	27.81171141644854	20.458603818563137	24.801333895736853	26.92835086925147
31	28.128724759814993	22.91703715243536	23.629619069594497	25.324619018155147
32	29.316532752901548	22.521542063423244	22.82988420268278	25.332040980992428
33	29.740907360676534	22.35539297456104	24.771425590046046	23.132274074716385
34	26.519408065395577	23.536219913493717	25.696231553482956	24.24814046762775
35	27.30789976088052	25.417871204694798	24.959252689175546	22.314976345249136
36	28.29450642477436	23.3001867983122	24.182959467264734	24.222347309648697
37	27.43907009419279	25.01069203557251	22.733398685798186	24.81683918443651
38	29.174339702504216	24.013136139374176	22.58319579431904	24.229328363802573
39	26.51499897856155	22.50669813774869	24.455955427071498	26.522347456618263
40	28.24321404793853	23.074588521971215	24.488435700082157	24.1937617300081
41	25.934248757739788	23.04431279237757	25.25715998959456	25.764278460288086
42	25.331453102747894	22.887496270647386	25.58438771745984	26.196662909144884
43	26.196222000461482	22.05065158954929	26.650064005243873	25.10306240474535
44	26.49368839219709	22.77881228018865	24.700880200701633	26.026619126912625
45	25.38531744690357	22.239727929948426	25.8178488653215	26.5571057578265
46	26.57165574437878	22.339079353275142	24.61813633778308	26.471128564562996
47	25.955632828884813	24.182739012923033	24.915382275176988	24.94624588301517
48	27.39203983462985	24.887017149878087	24.035916421349974	23.685026594142087
49	27.4459776635661	24.942204220083976	22.644188162189728	24.967629954160195
50	24.705877165780198	25.045376852000185	24.781713459325438	25.467032522894183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	2.5
10	3.0
11	90.0
12	177.0
13	1284.5
14	2392.0
15	2519.5
16	2647.0
17	3345.5
18	4044.0
19	4374.0
20	4704.0
21	3796.0
22	2888.0
23	3489.0
24	4090.0
25	4042.0
26	3994.0
27	4056.5
28	4119.0
29	5749.5
30	7380.0
31	10434.0
32	13488.0
33	15164.5
34	16841.0
35	16550.0
36	16259.0
37	19672.0
38	23085.0
39	24449.5
40	25814.0
41	29587.0
42	33360.0
43	35127.5
44	36895.0
45	41117.5
46	45340.0
47	47559.5
48	49779.0
49	53650.5
50	57522.0
51	60362.0
52	63202.0
53	68324.5
54	73447.0
55	80041.0
56	86635.0
57	86589.5
58	86544.0
59	99990.5
60	113437.0
61	120275.5
62	127114.0
63	122914.0
64	118714.0
65	106703.0
66	94692.0
67	87933.5
68	81175.0
69	67044.5
70	52914.0
71	45013.0
72	37112.0
73	32408.5
74	27705.0
75	24160.0
76	20615.0
77	17612.0
78	14609.0
79	10000.0
80	5391.0
81	3409.5
82	1428.0
83	1207.0
84	986.0
85	583.5
86	181.0
87	115.5
88	50.0
89	41.0
90	32.0
91	20.0
92	8.0
93	8.0
94	8.0
95	6.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1360826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.853388608320124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.55922961143574	6.069815024370889
2	11.059416079024063	1.9582661665740808
3	4.5789830744765965	1.2161854976778526
4	2.3191868618215894	0.8213065017206781
5	1.3850078444884397	0.613100633641398
6	0.8188184178502278	0.43495905917267486
7	0.509491995909412	0.31575114428302703
8	0.3740200334808383	0.2649075762962212
9	0.28967301932812595	0.23081290285115927
>10	3.9344383340880396	9.456759768602744
>50	3.056048104594656	19.748292175345867
>100	2.9648863685501543	46.18321413897597
>500	0.11075724939986209	6.6125585125409
>1k	0.03919102671072043	5.289835608839155
>5k	0.0	0.0
>10k+	8.519788415374007E-4	0.7842352891073819
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	10397	0.7640212635561049	No Hit
CGCTTGGGGGTGTGTTAGAGTGCTCATTTTTCTTTTGCTCTAGCGTAGAT	3522	0.2588133971573147	No Hit
CGCTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3030	0.22265888511830315	No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2482	0.18238922536753413	No Hit
CGCTTGGGGGAGCACGGCCCTGCTGAAGCAACACAGGTTTCCAACCTCTT	2349	0.17261574955211026	No Hit
CGCTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	2331	0.1712930235019025	No Hit
CGCTTGGGGACATAAAAGAATACATACTGGAGAGAAACCCTACAAATGTG	2232	0.16401803022575995	No Hit
CGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2065	0.1517460718710548	No Hit
CGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	1939	0.1424869895196006	No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1903	0.13984153741918512	No Hit
CGCTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1903	0.13984153741918512	No Hit
CGCTTGGGGAAGAGATGTGAAGAGAAACTCTCTACTCCTTATGTTGGCGC	1867	0.13719608531876962	No Hit
CGCTTGGGGAATACTTGTGTTTTAAAAGTAATTTTTATTCTTTGGCGGTT	1771	0.13014154638432834	No Hit
CGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1763	0.12955366813979158	No Hit
CGCTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1588	0.11669383154054964	No Hit
CGCTTGGGGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTGC	1584	0.11639989241828126	No Hit
CGCTTGGGACAACAAATAGATGCTATATTGCTTGATTAGCTTGGTGCAGT	1468	0.10787565787249802	No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1452	0.10669990138342449	No Hit
CGCTTGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGA	1451	0.10662641660285739	No Hit
CGCTTGGGGGCGTAGCACAGTGCACGGTGCCCTCAAGTTTGCCTTCGACT	1409	0.10354005581903931	No Hit
CGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	1396	0.10258475367166706	No Hit
CGCTTGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	1381	0.10148248196316062	No Hit
CGCTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT	1371	0.10074763415748964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.93939122268387E-4	0.0	0.0	0.0	0.0
9	0.0016901499530432252	0.0	0.0	0.0	0.0
10	0.0090386280097529	0.0	0.0	0.0	0.0
11	0.017783316897237413	0.0	0.0	0.0	0.0
12	0.022265888511830315	0.0	0.0	0.0	0.0
13	0.028365125298899344	0.0	0.0	0.0	0.0
14	0.04144541623984257	0.0	0.0	0.0	0.0
15	0.05151283117753482	0.0	0.0	0.0	0.0
16	0.06091888309012321	0.0	0.0	0.0	0.0
17	0.06944311763590642	0.0	0.0	0.0	0.0
18	0.07605674788694514	0.0	0.0	0.0	0.0
19	0.08627113238577158	0.0	0.0	0.0	0.0
20	0.09369309522304835	0.0	0.0	0.0	0.0
21	0.10008627113238577	0.0	0.0	0.0	0.0
22	0.10765520353079673	0.0	0.0	0.0	0.0
23	0.11338701641503028	0.0	0.0	0.0	0.0
24	0.1219847357413806	0.0	0.0	0.0	0.0
25	0.12764306384504706	0.0	0.0	0.0	0.0
26	0.13418320931551866	0.0	0.0	0.0	0.0
27	0.14131123303052706	0.0	0.0	0.0	0.0
28	0.15057031538198123	0.0	0.0	0.0	0.0
29	0.1581392477803922	0.0	0.0	0.0	0.0
30	0.16585514973993737	0.0	0.0	0.0	0.0
31	0.17305665823551283	0.0	0.0	0.0	0.0
32	0.18033165151165542	0.0	0.0	0.0	0.0
33	0.18665134264042574	0.0	0.0	0.0	0.0
34	0.19539603152791024	0.0	0.0	0.0	0.0
35	0.21148919847210446	0.0	0.0	0.0	0.0
36	0.22096873516525992	0.0	0.0	0.0	0.0
37	0.22721494151346314	0.0	0.0	0.0	0.0
38	0.23529826737584378	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCTA	55	1.8189894E-12	44.000004	43
ATTATCG	110	0.0	44.000004	11
ATAGTCG	55	1.8189894E-12	44.000004	22
TATCGCG	130	0.0	44.000004	13
AACGTCG	20	7.8569935E-4	44.0	19
AACCGGT	30	2.5280624E-6	44.0	16
GTACCGG	95	0.0	44.0	22
ACACGCG	20	7.8569935E-4	44.0	10
CGTATTA	20	7.8569935E-4	44.0	31
TAGACGA	30	2.5280624E-6	44.0	27
CACGCTA	80	0.0	44.0	28
ACCGATA	25	4.4432738E-5	44.0	44
TTCACGC	20	7.8569935E-4	44.0	12
ATAGCGG	20	7.8569935E-4	44.0	12
CGCGATA	25	4.4432738E-5	44.0	29
TAACGGG	20	7.8569935E-4	44.0	11
TAACGGC	35	1.4463512E-7	44.0	27
AATGCGT	25	4.4432738E-5	44.0	14
CTTATCG	25	4.4432738E-5	44.0	38
CGCTAAT	25	4.4432738E-5	44.0	12
>>END_MODULE