FastQCFastQC Report
Fri 17 Jun 2016
SRR1527254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469860
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA109480.7448328412229737No Hit
CGCTTGGGGGTGTGTTAGAGTGCTCATTTTTCTTTTGCTCTAGCGTAGATTT33620.22872926673288613No Hit
CGCTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT27800.1891336589879308No Hit
CGCTTGGGGACATAAAAGAATACATACTGGAGAGAAACCCTACAAATGTGAT25100.17076456261140516No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC24170.16443742941504635No Hit
CGCTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA21720.14776917529560638No Hit
CGCTTGGGGGAGCACGGCCCTGCTGAAGCAACACAGGTTTCCAACCTCTTGA20500.13946906508102813No Hit
CGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG19960.135795245805723No Hit
CGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC19180.13048861796361558No Hit
CGCTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG18960.12899187677738014No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT18880.12844760725511273No Hit
CGCTTGGGGAAGAGATGTGAAGAGAAACTCTCTACTCCTTATGTTGGCGCAC17750.12075980025308532No Hit
CGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC17290.11763025050004763No Hit
CGCTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG15890.10810553386036766No Hit
CGCTTGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC15870.10796946647980081No Hit
CGCTTGGGACAACAAATAGATGCTATATTGCTTGATTAGCTTGGTGCAGTTC15370.10456778196562938No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT15150.10307104077939394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC253.417625E-546.045
ACCGATA206.312032E-446.044
TTATCGG206.312032E-446.014
CCCGTTA206.312032E-446.037
ACTAACG253.417625E-546.025
TCGTAAG206.312032E-446.043
ATGCGCG253.417625E-546.037
CGGTCTA301.8617593E-646.020
TATCGCG800.046.013
TTCGAAC206.312032E-446.046
GCGTATA253.417625E-546.035
GCGTACC206.312032E-446.018
GTTATAT206.312032E-446.032
AATCGCT453.110472E-1046.010
TACGTAC206.312032E-446.043
GTCGATA1400.045.99999621
ATTCGAT351.0198346E-745.99999621
ACGTATA700.045.99999620
ATAGTCG351.0198346E-745.99999622
CGACTTA1250.044.1622
Produced by FastQC (version 0.11.5)