##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527254_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1469860
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.483718857578275	33.0	31.0	34.0	31.0	34.0
2	32.664179581728874	34.0	31.0	34.0	31.0	34.0
3	32.91291891744792	34.0	31.0	34.0	31.0	34.0
4	36.35765174914618	37.0	37.0	37.0	35.0	37.0
5	36.437225994312385	37.0	37.0	37.0	35.0	37.0
6	36.254910671764655	37.0	37.0	37.0	35.0	37.0
7	36.61248078048249	37.0	37.0	37.0	35.0	37.0
8	36.64706434626427	37.0	37.0	37.0	35.0	37.0
9	38.61578993917788	39.0	39.0	39.0	38.0	39.0
10	38.02514185024424	39.0	38.0	39.0	35.0	39.0
11	37.08063352972392	39.0	35.0	39.0	34.0	39.0
12	36.849201284476074	39.0	35.0	39.0	33.0	39.0
13	36.818411957601406	39.0	35.0	39.0	33.0	39.0
14	37.547984842093804	40.0	36.0	40.0	33.0	40.0
15	37.536531370334586	40.0	36.0	40.0	33.0	40.0
16	32.620682922183065	39.0	34.0	40.0	3.0	40.0
17	34.263493121793914	39.0	34.0	40.0	17.0	40.0
18	36.30336630699522	39.0	34.0	40.0	30.0	40.0
19	37.22896806498578	39.0	35.0	40.0	33.0	40.0
20	37.45727552283891	39.0	36.0	40.0	33.0	40.0
21	37.62811288149892	40.0	36.0	40.0	33.0	40.0
22	37.69403004367763	40.0	36.0	40.0	34.0	40.0
23	37.65779393955887	40.0	36.0	40.0	34.0	40.0
24	37.59928972827344	40.0	35.0	40.0	34.0	40.0
25	37.5310138380526	40.0	35.0	40.0	33.0	40.0
26	37.46161062958377	40.0	35.0	40.0	33.0	40.0
27	37.35564679629353	40.0	35.0	40.0	33.0	40.0
28	37.27134284897881	40.0	35.0	40.0	33.0	40.0
29	37.076341284203934	40.0	35.0	40.0	33.0	40.0
30	37.02911025539847	39.0	35.0	40.0	33.0	40.0
31	36.99276189569075	39.0	35.0	40.0	33.0	40.0
32	36.93665995400922	39.0	35.0	40.0	33.0	40.0
33	36.62336889227546	39.0	35.0	40.0	32.0	40.0
34	36.56103438422707	39.0	35.0	40.0	31.0	40.0
35	36.517104350074156	39.0	35.0	40.0	31.0	40.0
36	36.440844706298556	38.0	35.0	40.0	31.0	40.0
37	36.10887091287606	38.0	35.0	40.0	31.0	40.0
38	36.05184099165907	38.0	35.0	40.0	30.0	40.0
39	35.6946947328317	38.0	35.0	40.0	30.0	40.0
40	35.67648823697495	38.0	35.0	40.0	29.0	40.0
41	35.57494863456384	38.0	34.0	40.0	29.0	40.0
42	35.5806287673656	38.0	34.0	40.0	30.0	40.0
43	35.512929802838364	37.0	34.0	40.0	30.0	40.0
44	35.77280557331991	37.0	35.0	40.0	30.0	40.0
45	35.83934660443852	37.0	35.0	40.0	31.0	40.0
46	35.80770821710911	37.0	35.0	40.0	31.0	40.0
47	35.51996244540296	37.0	35.0	40.0	30.0	40.0
48	35.45399629896725	36.0	35.0	40.0	30.0	40.0
49	35.41362578748996	36.0	34.0	40.0	30.0	40.0
50	34.91473405630468	36.0	34.0	40.0	29.0	40.0
51	34.94515940293633	35.0	34.0	40.0	29.0	40.0
52	34.64992108091927	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	22.0
16	62.0
17	150.0
18	263.0
19	519.0
20	972.0
21	1512.0
22	2368.0
23	3445.0
24	4823.0
25	6812.0
26	8901.0
27	12034.0
28	15484.0
29	19976.0
30	26335.0
31	36025.0
32	50944.0
33	78733.0
34	119907.0
35	135250.0
36	157329.0
37	253831.0
38	346491.0
39	187667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.3575578626536	0.5716870994516484	0.053610547943341545	0.017144489951423945
8	99.14563291742071	0.6333936565387179	0.08027975453444545	0.14069367150612982
9	93.19118827643449	3.5682309879852507	1.859700923897514	1.3808798116827452
10	48.73028723824038	34.91373328072061	7.011279985848992	9.344699495190017
11	32.844692691820995	24.401235491815548	24.635611554841958	18.118460261521506
12	30.408678377532556	16.670567264909582	28.226497761691586	24.694256595866275
13	24.583769882845985	17.018083354877337	30.85865320506715	27.539493557209532
14	24.25224171009484	20.184235233287524	30.85225803818051	24.711265018437132
15	25.166138271672132	20.288871048943435	28.55993087777067	25.985059801613758
16	27.747472548405973	28.562380090620877	22.569020178792538	21.121127182180615
17	28.755663804716097	21.2037200821847	24.49212850203421	25.548487611065003
18	27.942048902616573	19.43225885458479	26.027852992802035	26.597839249996596
19	28.797164355788986	21.78323105601894	23.49284965915121	25.92675492904086
20	27.06318969153525	22.471732001687236	25.54250064632006	24.92257766045746
21	29.57397303144517	18.96752071625869	27.579157198644772	23.87934905365137
22	28.90989618058863	19.232375872532078	26.137931503680623	25.71979644319867
23	26.71832691548855	20.512633856285632	26.319105220905392	26.449934007320426
24	24.80433510674486	23.297661001728056	25.889948702597525	26.008055188929557
25	27.57990556923789	23.197107207489147	24.283605241315502	24.939381981957464
26	26.993114990543315	23.593471487080404	23.57142857142857	25.84198495094771
27	26.485039391506675	20.565700134706706	25.638972419141957	27.31028805464466
28	27.999877539357488	20.478548977453634	24.23713823085192	27.28443525233696
29	28.152545140353503	21.95726123576395	23.87581130175663	26.01438232212592
30	27.913610820078105	20.29451784523696	24.8325010545222	26.959370280162737
31	28.262283482780674	22.688419305239954	23.663137985930632	25.386159226048736
32	29.374226116773027	22.140203828936087	22.850135387043665	25.63543466724722
33	29.984012082783394	22.170070618970513	24.70214850393915	23.14376879430694
34	26.669682826935897	23.483324942511533	25.639652756044796	24.207339474507776
35	27.455880151851197	25.35268665042929	24.81120650946349	22.380226688256023
36	28.248268542582288	23.170710135659178	24.25822867483978	24.322792646918753
37	27.578680962812786	24.913189011198345	22.74706434626427	24.7610656797246
38	29.033173227382196	23.98752262120202	22.498197107207492	24.48110704420829
39	26.82602424720722	22.470915597403835	24.279523219898493	26.423536935490453
40	28.37120542092444	22.94946457485747	24.44736233382771	24.23196767039038
41	25.85150966758739	23.030696801055882	25.21029213666608	25.90750139469065
42	25.34765215734832	22.77482209189991	25.48127032506497	26.3962554256868
43	26.14269386200046	22.00706189705142	26.555114092498606	25.295130148449513
44	26.424013171322443	22.681343801450478	24.69759024669016	26.19705278053692
45	25.44738954730382	22.140067761555525	25.80327378117644	26.60926890996421
46	26.271481637706994	22.289809913869348	24.841141333188194	26.597567115235464
47	26.13663886356524	24.154545330847835	24.92080878451009	24.788007021076837
48	27.53786074864273	24.757119725688163	23.97582082647327	23.72919869919584
49	27.505952947899797	24.794130053202345	22.673179758616467	25.02673724028139
50	24.696841876097043	25.017212523641707	24.668743962009987	25.617201638251263
51	24.754126243315692	25.948253575170426	24.064536758602863	25.233083422911022
52	26.224878559862848	24.321363939422803	24.212101832827614	25.241655667886736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	2.0
10	3.0
11	91.0
12	179.0
13	1266.5
14	2546.5
15	2739.0
16	3502.5
17	4266.0
18	4407.5
19	4549.0
20	3780.5
21	3012.0
22	3430.5
23	3849.0
24	3833.5
25	3818.0
26	3878.5
27	3939.0
28	5551.0
29	7163.0
30	8318.0
31	9473.0
32	11904.5
33	14336.0
34	16256.0
35	18176.0
36	18150.5
37	18125.0
38	22172.0
39	28345.0
40	30471.0
41	33304.5
42	36138.0
43	41234.5
44	46331.0
45	43484.0
46	40637.0
47	45711.5
48	50786.0
49	55612.0
50	60438.0
51	65190.5
52	69943.0
53	71521.0
54	73099.0
55	79635.5
56	86172.0
57	93052.0
58	99932.0
59	111911.5
60	123891.0
61	126836.0
62	129781.0
63	121969.5
64	109433.0
65	104708.0
66	95980.0
67	87252.0
68	74951.0
69	62650.0
70	52610.5
71	42571.0
72	38055.0
73	33539.0
74	28514.5
75	23490.0
76	21090.0
77	18690.0
78	13266.0
79	7842.0
80	5490.0
81	3138.0
82	2153.0
83	1168.0
84	882.5
85	597.0
86	384.0
87	171.0
88	103.5
89	24.5
90	13.0
91	12.5
92	12.0
93	8.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1469860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.617385792324313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.24010462339055	7.77618446256733
2	9.948354826803547	2.112510423902099
3	3.7139225621539222	1.1829644593561728
4	1.8444148154191375	0.7833145462553448
5	1.090283305111447	0.5787979236649335
6	0.6871715701194292	0.43775793992851314
7	0.40118987512625365	0.29817113761328956
8	0.3021828074582069	0.25667131572731516
9	0.21704169233171816	0.2073973842454329
>10	3.390848155364761	9.795757180352734
>50	2.6316310272974768	20.33388271337489
>100	2.4174955711036823	44.53358081012879
>500	0.08507738663591842	6.1311066472394335
>1k	0.029560786881971658	4.733824900847018
>5k	0.0	0.0
>10k+	7.209948019993087E-4	0.8380781547967002
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	10948	0.7448328412229737	No Hit
CGCTTGGGGGTGTGTTAGAGTGCTCATTTTTCTTTTGCTCTAGCGTAGATTT	3362	0.22872926673288613	No Hit
CGCTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2780	0.1891336589879308	No Hit
CGCTTGGGGACATAAAAGAATACATACTGGAGAGAAACCCTACAAATGTGAT	2510	0.17076456261140516	No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2417	0.16443742941504635	No Hit
CGCTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2172	0.14776917529560638	No Hit
CGCTTGGGGGAGCACGGCCCTGCTGAAGCAACACAGGTTTCCAACCTCTTGA	2050	0.13946906508102813	No Hit
CGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1996	0.135795245805723	No Hit
CGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1918	0.13048861796361558	No Hit
CGCTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1896	0.12899187677738014	No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1888	0.12844760725511273	No Hit
CGCTTGGGGAAGAGATGTGAAGAGAAACTCTCTACTCCTTATGTTGGCGCAC	1775	0.12075980025308532	No Hit
CGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1729	0.11763025050004763	No Hit
CGCTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1589	0.10810553386036766	No Hit
CGCTTGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC	1587	0.10796946647980081	No Hit
CGCTTGGGACAACAAATAGATGCTATATTGCTTGATTAGCTTGGTGCAGTTC	1537	0.10456778196562938	No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1515	0.10307104077939394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.360673805668567E-4	0.0	0.0	0.0	0.0
9	8.164042834011403E-4	0.0	0.0	0.0	0.0
10	0.008912413427129114	0.0	0.0	0.0	0.0
11	0.016736287809723374	0.0	0.0	0.0	0.0
12	0.02068224184616222	0.0	0.0	0.0	0.0
13	0.02490033064373478	0.0	0.0	0.0	0.0
14	0.039799708815805586	0.0	0.0	0.0	0.0
15	0.04830392010123413	0.0	0.0	0.0	0.0
16	0.05959751268828324	0.0	0.0	0.0	0.0
17	0.06687711754861007	0.0	0.0	0.0	0.0
18	0.0729321159838352	0.0	0.0	0.0	0.0
19	0.08272896738464888	0.0	0.0	0.0	0.0
20	0.089736437483842	0.0	0.0	0.0	0.0
21	0.09701604234416883	0.0	0.0	0.0	0.0
22	0.10504401779761338	0.0	0.0	0.0	0.0
23	0.11218755527737335	0.0	0.0	0.0	0.0
24	0.11933109275713333	0.0	0.0	0.0	0.0
25	0.12531805750207503	0.0	0.0	0.0	0.0
26	0.13252962867211843	0.0	0.0	0.0	0.0
27	0.13967316615187841	0.0	0.0	0.0	0.0
28	0.14974215231382582	0.0	0.0	0.0	0.0
29	0.15804226252840406	0.0	0.0	0.0	0.0
30	0.16641040643326574	0.0	0.0	0.0	0.0
31	0.1732137754616086	0.0	0.0	0.0	0.0
32	0.18096961615391943	0.0	0.0	0.0	0.0
33	0.1874328167308451	0.0	0.0	0.0	0.0
34	0.19491652266202222	0.0	0.0	0.0	0.0
35	0.20859129440899132	0.0	0.0	0.0	0.0
36	0.21729960676527016	0.0	0.0	0.0	0.0
37	0.22267426829766102	0.0	0.0	0.0	0.0
38	0.2300899405385547	0.0	0.0	0.0	0.0
39	0.23839005075313296	0.0	0.0	0.0	0.0
40	0.24607785775516036	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	25	3.417625E-5	46.0	45
ACCGATA	20	6.312032E-4	46.0	44
TTATCGG	20	6.312032E-4	46.0	14
CCCGTTA	20	6.312032E-4	46.0	37
ACTAACG	25	3.417625E-5	46.0	25
TCGTAAG	20	6.312032E-4	46.0	43
ATGCGCG	25	3.417625E-5	46.0	37
CGGTCTA	30	1.8617593E-6	46.0	20
TATCGCG	80	0.0	46.0	13
TTCGAAC	20	6.312032E-4	46.0	46
GCGTATA	25	3.417625E-5	46.0	35
GCGTACC	20	6.312032E-4	46.0	18
GTTATAT	20	6.312032E-4	46.0	32
AATCGCT	45	3.110472E-10	46.0	10
TACGTAC	20	6.312032E-4	46.0	43
GTCGATA	140	0.0	45.999996	21
ATTCGAT	35	1.0198346E-7	45.999996	21
ACGTATA	70	0.0	45.999996	20
ATAGTCG	35	1.0198346E-7	45.999996	22
CGACTTA	125	0.0	44.16	22
>>END_MODULE