FastQCFastQC Report
Fri 17 Jun 2016
SRR1527253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417437
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA106840.7537548406031449No Hit
CGCTTGGGGGTGTGTTAGAGTGCTCATTTTTCTTTTGCTCTAGCGTAGATTT32470.22907543686244963No Hit
CGCTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT28480.20092603762989114No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC22510.15880776359019835No Hit
CGCTTGGGGACATAAAAGAATACATACTGGAGAGAAACCCTACAAATGTGAT21810.15386927249676705No Hit
CGCTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA21040.1484369322939926No Hit
CGCTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG19580.13813665087055016No Hit
CGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC19380.1367256534152841No Hit
CGCTTGGGGGAGCACGGCCCTGCTGAAGCAACACAGGTTTCCAACCTCTTGA18560.1309405638486931No Hit
CGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG18140.1279774691926343No Hit
CGCTTGGGGAAGAGATGTGAAGAGAAACTCTCTACTCCTTATGTTGGCGCAC17980.12684867122842144No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT17940.12656647173736824No Hit
CGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC17410.12282732848091309No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT14700.10370831296205758No Hit
CGCTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG14510.10236786537955479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGT551.8189894E-1246.00000414
GTCGATA1600.046.00000421
CGTTGAC206.3119543E-446.00000419
ATCGTTC405.6134013E-946.00000424
CCCGTTA351.0197982E-746.00000437
TCAATCG206.3119543E-446.00000412
CTTATCG206.3119543E-446.00000438
TATTCGA700.046.00000418
CGAATAC351.0197982E-746.00000418
TTACGAT206.3119543E-446.00000441
CAGTACG405.6134013E-946.00000433
CGTTTAG206.3119543E-446.00000442
CGATAGA206.3119543E-446.00000431
CGATTGT301.861712E-646.012
TCGATAG301.861712E-646.022
ATCGTCA301.861712E-646.022
CATAACG253.4175624E-546.045
CGCGTAC253.4175624E-546.024
TTCGATA301.861712E-646.020
TCGCGTA253.4175624E-546.023
Produced by FastQC (version 0.11.5)