##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527253_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1417437
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45669472435107	33.0	31.0	34.0	31.0	34.0
2	32.66525284721649	34.0	31.0	34.0	31.0	34.0
3	32.915643517136914	34.0	31.0	34.0	31.0	34.0
4	36.37109726922608	37.0	37.0	37.0	35.0	37.0
5	36.451797857682564	37.0	37.0	37.0	35.0	37.0
6	36.236709638594164	37.0	37.0	37.0	35.0	37.0
7	36.6176761295211	37.0	37.0	37.0	35.0	37.0
8	36.671896528734614	37.0	37.0	37.0	35.0	37.0
9	38.558429051873205	39.0	39.0	39.0	38.0	39.0
10	38.01495375103091	39.0	38.0	39.0	35.0	39.0
11	36.99702279536939	39.0	35.0	39.0	34.0	39.0
12	36.79937168283317	39.0	35.0	39.0	33.0	39.0
13	36.719471129933815	39.0	35.0	39.0	33.0	39.0
14	37.47620105867139	40.0	36.0	40.0	33.0	40.0
15	37.65394581910871	40.0	36.0	40.0	33.0	40.0
16	37.80613671013244	40.0	36.0	40.0	34.0	40.0
17	37.81650048644137	40.0	36.0	40.0	34.0	40.0
18	37.81446723910833	40.0	36.0	40.0	34.0	40.0
19	37.78565749306671	40.0	36.0	40.0	34.0	40.0
20	37.70223085752665	40.0	36.0	40.0	33.0	40.0
21	33.40507408794888	39.0	34.0	40.0	10.0	40.0
22	34.14029477147838	39.0	34.0	40.0	16.0	40.0
23	36.19885610436302	39.0	34.0	40.0	30.0	40.0
24	36.968975693452336	39.0	35.0	40.0	32.0	40.0
25	37.23983570345631	39.0	35.0	40.0	33.0	40.0
26	37.34597304853761	40.0	35.0	40.0	33.0	40.0
27	37.33277105084741	40.0	35.0	40.0	33.0	40.0
28	37.25360844961716	40.0	35.0	40.0	33.0	40.0
29	37.22561284910723	40.0	35.0	40.0	33.0	40.0
30	37.10391290759307	39.0	35.0	40.0	33.0	40.0
31	36.9677389541828	39.0	35.0	40.0	33.0	40.0
32	36.94663748723929	39.0	35.0	40.0	33.0	40.0
33	36.652845946592336	39.0	35.0	40.0	32.0	40.0
34	36.58508632129682	39.0	35.0	40.0	31.0	40.0
35	36.5279959532593	39.0	35.0	40.0	31.0	40.0
36	36.388259231274475	38.0	35.0	40.0	31.0	40.0
37	36.27135809210568	38.0	35.0	40.0	31.0	40.0
38	36.14513025975758	38.0	35.0	40.0	31.0	40.0
39	36.005795672047505	38.0	35.0	40.0	30.0	40.0
40	31.164077839085618	36.0	30.0	40.0	8.0	40.0
41	31.98385748361303	35.0	30.0	40.0	13.0	40.0
42	33.83004324001702	35.0	31.0	40.0	23.0	40.0
43	34.740253005953704	36.0	33.0	40.0	27.0	40.0
44	35.46258352223062	37.0	34.0	40.0	30.0	40.0
45	35.713754473743805	37.0	35.0	40.0	30.0	40.0
46	35.784895554440865	37.0	35.0	40.0	31.0	40.0
47	35.73703804825188	37.0	35.0	40.0	31.0	40.0
48	35.57071460671621	36.0	35.0	40.0	30.0	40.0
49	35.47620952465612	36.0	35.0	40.0	30.0	40.0
50	35.3745598569813	36.0	34.0	40.0	30.0	40.0
51	35.14913608153308	36.0	34.0	40.0	30.0	40.0
52	34.78902272199752	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	3.0
15	22.0
16	57.0
17	140.0
18	339.0
19	572.0
20	1017.0
21	1570.0
22	2348.0
23	3635.0
24	5052.0
25	7073.0
26	9279.0
27	12449.0
28	16064.0
29	21171.0
30	28196.0
31	41056.0
32	58699.0
33	67306.0
34	128749.0
35	153161.0
36	170128.0
37	170952.0
38	329801.0
39	188595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.3430395848281	0.5848584452077941	0.056580997956170186	0.015520972007926985
8	99.15516527365943	0.6434148396013367	0.05947354273946567	0.1419463439997686
9	93.14417501448035	3.563967922383852	1.901389620843819	1.3904674422919678
10	48.695215378179064	34.95358171121538	7.003344769467708	9.347858141137841
11	32.969507639492974	24.342104799013995	24.596225440707418	18.092162120785616
12	30.389428242666167	16.735135318183453	28.22933223839931	24.646104200751072
13	24.720393216770834	17.04075736699409	30.71296995915868	27.525879457076396
14	24.19402061608382	20.214796142615157	30.913895996788572	24.677287244512456
15	25.196745957668664	20.114474223545738	28.645999786939385	26.042780031846213
16	28.96425026297465	20.796832592912416	25.6869264736281	24.551990670484823
17	28.7834309390823	21.163691931281605	24.41074982521269	25.642127304423408
18	27.894079243028084	19.45906590557464	25.982036591397005	26.664818260000267
19	28.86562154085155	21.742765286922804	23.58785610930151	25.803757062924138
20	27.053477509053316	22.445159820154263	25.55337556448717	24.947987106305252
21	34.07904548844146	18.565410667281864	25.80904830338138	21.54649554089529
22	28.916064699877314	19.273590290079913	26.073398676625487	25.736946333417286
23	26.64435879689891	20.572836746888925	26.30825920305453	26.47454525315764
24	24.966541722842003	23.20067840757649	25.893708150697346	25.939071718884154
25	27.640311350698475	23.161523228192856	24.26047859622685	24.937686824881812
26	27.02264721465575	23.590466454593752	23.61762815560762	25.769258175142877
27	26.4921121714757	20.537420710761747	25.591895795015933	27.37857132274662
28	27.98579407762038	20.493609239775736	24.22414541175375	27.29645127085013
29	28.009781034359904	21.99286458586872	23.873794743611178	26.1235596361602
30	27.937114665413702	20.265450951259208	24.862127911152314	26.935306472174776
31	28.292262724904177	22.719951574567336	23.628775035504223	25.359010665024268
32	29.34987586749887	22.142077566763106	22.87678394172016	25.631262624017857
33	29.976217637891487	22.277321672850363	24.596578190071234	23.149882499186912
34	26.6830201271732	23.449931108049245	25.64438490035183	24.22266386442572
35	27.411518113327084	25.447832954833267	24.809144956707073	22.33150397513258
36	28.347221075786788	23.195387167119243	24.15006804535228	24.307323711741684
37	27.381604967275443	24.994761671947323	22.80545802035646	24.818175340420773
38	29.029015046171363	24.01976243035846	22.444877620663213	24.50634490280697
39	26.60788451268028	22.415881622957492	24.294906934135344	26.681326930226884
40	28.649880029941365	27.450249993474134	22.305471072082923	21.594398904501574
41	25.839596398287895	23.125472243210808	25.064958795346815	25.96997256315448
42	25.407690077230942	22.843484401775882	25.449455601906823	26.299369919086352
43	26.205538588311157	22.025035327848784	26.49232382109399	25.27710226274607
44	26.32088763027916	22.75374496362096	24.783676452639515	26.141690953460365
45	25.468927366789494	22.13911447210705	25.794303379973854	26.597654781129602
46	26.28264959924145	22.337359614571938	24.80589966255996	26.57409112362666
47	25.933216079444797	24.122906344338407	24.94191981724761	25.001957758969183
48	27.451590441056638	24.800820071721	23.996622072092094	23.750967415130265
49	27.473319801867735	24.912853269669128	22.581179974841916	25.032646953621217
50	24.68187298624207	25.00336875642445	24.75326945747853	25.56148879985495
51	24.777115314472532	25.972865107937775	24.01983298023122	25.230186597358472
52	26.19044091553981	24.425424198747457	24.2042503476345	25.179884538078234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	83.5
12	165.0
13	1186.0
14	2357.0
15	2507.0
16	3288.5
17	4070.0
18	4277.0
19	4484.0
20	3699.0
21	2914.0
22	3289.0
23	3664.0
24	3684.5
25	3705.0
26	3662.0
27	3619.0
28	5128.5
29	6638.0
30	7926.0
31	9214.0
32	11388.0
33	13562.0
34	15273.0
35	16984.0
36	16913.0
37	16842.0
38	20841.0
39	27010.5
40	29181.0
41	32010.0
42	34839.0
43	39793.5
44	44748.0
45	41717.0
46	38686.0
47	43498.0
48	48310.0
49	53137.0
50	57964.0
51	63032.0
52	68100.0
53	69161.5
54	70223.0
55	76800.0
56	83377.0
57	88771.0
58	94165.0
59	107246.5
60	120328.0
61	123137.5
62	125947.0
63	118372.5
64	106350.0
65	101902.0
66	93461.0
67	85020.0
68	73295.0
69	61570.0
70	51268.5
71	40967.0
72	36781.5
73	32596.0
74	27369.0
75	22142.0
76	20196.5
77	18251.0
78	13008.0
79	7765.0
80	5470.0
81	3175.0
82	2196.0
83	1217.0
84	878.5
85	540.0
86	339.5
87	139.0
88	92.0
89	30.0
90	15.0
91	10.5
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1417437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.482596488450799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99823045560007	7.547283977485446
2	9.17064960295284	1.9226443864945233
3	3.8669654234604667	1.2160751450678204
4	2.1436323844410428	0.8988333322268436
5	1.2645167371508594	0.662770935422243
6	0.8646351635326007	0.5438172917422753
7	0.5512970075667494	0.40453168529288475
8	0.4197840088537508	0.35203411016945013
9	0.3397248813049958	0.3205078963026402
>10	4.169393851433705	11.53750948721182
>50	2.759965129424563	21.068789360112515
>100	2.338723186804512	42.35282476537041
>500	0.08455035407427546	6.041845698825234
>1k	0.027176899523874254	4.28505812447691
>5k	0.0	0.0
>10k+	7.549138756631738E-4	0.8454738037989863
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	10684	0.7537548406031449	No Hit
CGCTTGGGGGTGTGTTAGAGTGCTCATTTTTCTTTTGCTCTAGCGTAGATTT	3247	0.22907543686244963	No Hit
CGCTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2848	0.20092603762989114	No Hit
CGCTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2251	0.15880776359019835	No Hit
CGCTTGGGGACATAAAAGAATACATACTGGAGAGAAACCCTACAAATGTGAT	2181	0.15386927249676705	No Hit
CGCTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2104	0.1484369322939926	No Hit
CGCTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1958	0.13813665087055016	No Hit
CGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1938	0.1367256534152841	No Hit
CGCTTGGGGGAGCACGGCCCTGCTGAAGCAACACAGGTTTCCAACCTCTTGA	1856	0.1309405638486931	No Hit
CGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1814	0.1279774691926343	No Hit
CGCTTGGGGAAGAGATGTGAAGAGAAACTCTCTACTCCTTATGTTGGCGCAC	1798	0.12684867122842144	No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1794	0.12656647173736824	No Hit
CGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1741	0.12282732848091309	No Hit
CGCTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1470	0.10370831296205758	No Hit
CGCTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1451	0.10236786537955479	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.4109974552660895E-4	0.0	0.0	0.0	0.0
9	6.349488548697402E-4	0.0	0.0	0.0	0.0
10	0.00931258320475619	0.0	0.0	0.0	0.0
11	0.016508670226613245	0.0	0.0	0.0	0.0
12	0.020600562846884905	0.0	0.0	0.0	0.0
13	0.025468504067552914	0.0	0.0	0.0	0.0
14	0.038096931292184416	0.0	0.0	0.0	0.0
15	0.04656291602378095	0.0	0.0	0.0	0.0
16	0.05693374731998671	0.0	0.0	0.0	0.0
17	0.06271883688657767	0.0	0.0	0.0	0.0
18	0.0677984277255356	0.0	0.0	0.0	0.0
19	0.07527671423844587	0.0	0.0	0.0	0.0
20	0.08070905444122031	0.0	0.0	0.0	0.0
21	0.08642359413504798	0.0	0.0	0.0	0.0
22	0.09488957886664451	0.0	0.0	0.0	0.0
23	0.10180346639744835	0.0	0.0	0.0	0.0
24	0.11005780151075498	0.0	0.0	0.0	0.0
25	0.11683058929603221	0.0	0.0	0.0	0.0
26	0.12402667631788926	0.0	0.0	0.0	0.0
27	0.1307289142304032	0.0	0.0	0.0	0.0
28	0.14067644629002912	0.0	0.0	0.0	0.0
29	0.14900133127609905	0.0	0.0	0.0	0.0
30	0.1561974182979561	0.0	0.0	0.0	0.0
31	0.16219415748283697	0.0	0.0	0.0	0.0
32	0.170307392850617	0.0	0.0	0.0	0.0
33	0.17715073050865754	0.0	0.0	0.0	0.0
34	0.18575781498578067	0.0	0.0	0.0	0.0
35	0.19888009131975531	0.0	0.0	0.0	0.0
36	0.20657002745095548	0.0	0.0	0.0	0.0
37	0.2129900658724162	0.0	0.0	0.0	0.0
38	0.22216154933164578	0.0	0.0	0.0	0.0
39	0.23090973355429553	0.0	0.0	0.0	0.0
40	0.23944626815865538	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGT	55	1.8189894E-12	46.000004	14
GTCGATA	160	0.0	46.000004	21
CGTTGAC	20	6.3119543E-4	46.000004	19
ATCGTTC	40	5.6134013E-9	46.000004	24
CCCGTTA	35	1.0197982E-7	46.000004	37
TCAATCG	20	6.3119543E-4	46.000004	12
CTTATCG	20	6.3119543E-4	46.000004	38
TATTCGA	70	0.0	46.000004	18
CGAATAC	35	1.0197982E-7	46.000004	18
TTACGAT	20	6.3119543E-4	46.000004	41
CAGTACG	40	5.6134013E-9	46.000004	33
CGTTTAG	20	6.3119543E-4	46.000004	42
CGATAGA	20	6.3119543E-4	46.000004	31
CGATTGT	30	1.861712E-6	46.0	12
TCGATAG	30	1.861712E-6	46.0	22
ATCGTCA	30	1.861712E-6	46.0	22
CATAACG	25	3.4175624E-5	46.0	45
CGCGTAC	25	3.4175624E-5	46.0	24
TTCGATA	30	1.861712E-6	46.0	20
TCGCGTA	25	3.4175624E-5	46.0	23
>>END_MODULE