FastQCFastQC Report
Fri 17 Jun 2016
SRR1527252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456776
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC68150.4678138574496011No Hit
GACACTGGGATCCAAATCAAATGTAAGAAGTCCCTCACATCCGATTAGCC65220.4477009505922668No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT58720.40308187394630335No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC51070.35056865297066947No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC42970.2949664189964689No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC36130.2480134214182551No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA29380.20167822643975464No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT24250.16646347825609428No Hit
GACACTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCT23660.16241343899130684No Hit
GACACTGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGC23160.15898120232623272No Hit
GACACTGGGGCTGACACTGGGGAATCTCTCTTCATCTCTAACCATGCCTA22540.15472522886154083No Hit
GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT21980.15088112379665783No Hit
GACACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC21940.15060654486345187No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG20300.13934880860200882No Hit
GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT19560.13426909833769912No Hit
GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC19260.13220975633865467No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG18900.12973854593980133No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC18850.12939532227329392No Hit
GACACTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA18560.12740462500755093No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG17890.12280542787635161No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC17400.11944183594457898No Hit
GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC17350.11909861227807157No Hit
GACACTGGGACAGTGCTGGGACCATGGTTTGTTGGTGAGATAATTGATGG17150.11772571761204194No Hit
GACACTGGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCC17000.11669604661251969No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGA16680.11449941514687226No Hit
GACACTGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG16640.11422483621366633No Hit
GACACTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA16520.11340109941404856No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG16510.11333245468074708No Hit
GACACTGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT15620.10722307341691517No Hit
GACACTGGGGCACTAATTAGACTGATGATCATTGTGTGGACATCTCCTGG15170.10413406041834847No Hit
GACACTGGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG14850.10193742895270104No Hit
GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG14620.10035860008676693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATC207.857175E-444.00000444
CTATACG207.857175E-444.00000426
GACAACG207.857175E-444.00000426
ACCGATA207.857175E-444.00000422
ATTGTCG207.857175E-444.00000442
TACGGAC207.857175E-444.00000428
TGCGAAA207.857175E-444.00000427
TCGTCAA207.857175E-444.00000443
CGTACTT207.857175E-444.00000420
CGCAATT207.857175E-444.00000431
ATACGGT207.857175E-444.00000431
GATTACG408.314601E-944.00000423
TATCGCC207.857175E-444.00000441
GCGTACG207.857175E-444.00000442
TAATCGT207.857175E-444.00000421
TCACGAA302.5281843E-644.018
CCTTACG254.443428E-544.018
TCGCAAT351.4464422E-744.038
ACACGCA254.443428E-544.011
CATATCG302.5281843E-644.023
Produced by FastQC (version 0.11.5)