##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527252_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1456776 Sequences flagged as poor quality 0 Sequence length 50 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.652078287945436 34.0 31.0 34.0 31.0 34.0 2 32.728501155977305 34.0 31.0 34.0 31.0 34.0 3 32.98917403911102 34.0 31.0 34.0 31.0 34.0 4 36.39155367743565 37.0 37.0 37.0 35.0 37.0 5 36.45507339494885 37.0 37.0 37.0 35.0 37.0 6 36.33385228751709 37.0 37.0 37.0 35.0 37.0 7 36.55273357056953 37.0 37.0 37.0 35.0 37.0 8 36.67768895149288 37.0 37.0 37.0 35.0 37.0 9 38.7719855351818 39.0 39.0 39.0 39.0 39.0 10 38.28272363081215 39.0 39.0 39.0 37.0 39.0 11 37.974018654892724 39.0 38.0 39.0 35.0 39.0 12 37.53234127964766 39.0 37.0 39.0 35.0 39.0 13 37.3775096514495 39.0 37.0 39.0 35.0 39.0 14 38.19799955518213 40.0 38.0 40.0 35.0 40.0 15 38.22091728584216 40.0 38.0 40.0 34.0 40.0 16 38.11797283865193 40.0 38.0 40.0 34.0 40.0 17 38.19104653014602 40.0 38.0 40.0 34.0 40.0 18 38.18193325535292 40.0 38.0 40.0 34.0 40.0 19 38.131300900069746 40.0 38.0 40.0 34.0 40.0 20 37.90363789628604 40.0 38.0 40.0 34.0 40.0 21 38.01417582387409 40.0 38.0 40.0 34.0 40.0 22 37.8272280707535 40.0 38.0 40.0 34.0 40.0 23 37.871157954277116 40.0 38.0 40.0 34.0 40.0 24 37.89278928263508 40.0 38.0 40.0 34.0 40.0 25 37.80666348155104 40.0 37.0 40.0 34.0 40.0 26 37.69904638736497 40.0 37.0 40.0 33.0 40.0 27 37.610960092697844 40.0 37.0 40.0 33.0 40.0 28 37.43277278044119 40.0 37.0 40.0 33.0 40.0 29 37.675996858817 40.0 37.0 40.0 33.0 40.0 30 37.700997270685406 40.0 37.0 40.0 33.0 40.0 31 37.65890500667227 40.0 37.0 40.0 33.0 40.0 32 36.94224644008413 40.0 36.0 40.0 31.0 40.0 33 37.1930296764911 40.0 36.0 40.0 32.0 40.0 34 37.13708010016639 40.0 36.0 40.0 32.0 40.0 35 37.173602530519446 40.0 36.0 40.0 32.0 40.0 36 36.95649022224419 40.0 35.0 40.0 32.0 40.0 37 36.87997948895369 40.0 35.0 40.0 31.0 40.0 38 36.68166965957704 40.0 35.0 40.0 31.0 40.0 39 36.63337740325211 39.0 35.0 40.0 31.0 40.0 40 36.50393334321817 39.0 35.0 40.0 31.0 40.0 41 36.36187100830876 39.0 35.0 40.0 31.0 40.0 42 36.25814401115889 39.0 35.0 40.0 30.0 40.0 43 36.137339577258274 39.0 35.0 40.0 30.0 40.0 44 35.970469722181036 39.0 35.0 40.0 30.0 40.0 45 35.84569075822227 38.0 35.0 40.0 30.0 40.0 46 35.5494290131084 38.0 35.0 40.0 29.0 40.0 47 35.502881019456666 38.0 35.0 40.0 29.0 40.0 48 35.33970562392571 37.0 34.0 40.0 28.0 40.0 49 35.008971866642504 37.0 34.0 40.0 27.0 40.0 50 34.67603667276232 36.0 34.0 40.0 26.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 2.0 15 10.0 16 36.0 17 71.0 18 165.0 19 354.0 20 690.0 21 1213.0 22 1799.0 23 2654.0 24 4039.0 25 6049.0 26 7699.0 27 10287.0 28 13932.0 29 17664.0 30 22957.0 31 29989.0 32 36335.0 33 46814.0 34 78401.0 35 96860.0 36 120094.0 37 216049.0 38 456330.0 39 286282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.52861661641872 0.4131726497416212 0.03878427431533743 0.019426459524319456 8 99.5039731571635 0.4055530843451567 0.048806405377353826 0.04166735311399968 9 98.92625908169822 0.48531826444147896 0.19014591124510563 0.3982767426151996 10 54.3858493000983 28.660754982234742 5.883265512336831 11.070130205330127 11 33.44467509074834 24.63927192650071 21.762439798568895 20.153613184182056 12 29.255492951558786 16.166383850365467 28.65313541683828 25.924987781237473 13 22.72888899872046 17.198663349753154 30.88415789386975 29.188289757656634 14 23.464485960779143 19.93189069561827 32.03313343986996 24.570489903732625 15 25.134131808871096 20.492031719358366 28.033204830392595 26.34063164137795 16 27.970394899421734 22.285375376859587 24.641605847432963 25.10262387628572 17 29.541741489425966 19.523248598274545 22.792934534890747 28.142075377408744 18 27.38506125856 20.182787195835186 24.666798464554606 27.765353081050208 19 28.50184242464181 19.51597225654459 24.14839343866181 27.833791880151786 20 29.49492578131435 20.606050621372127 24.503286709830473 25.395736887483046 21 32.39811748683394 18.67123016853655 25.090405113758052 23.84024723087146 22 31.141232420083803 18.916635090089347 24.760635814977732 25.181496674849118 23 29.81137800183419 22.579312124856532 23.07904578329132 24.53026409001796 24 28.730292097069142 22.06660461182776 23.444029830255303 25.75907346084779 25 27.077670142835963 22.50188086569246 23.32946177037513 27.090987221096448 26 27.228482621899317 22.302811139118162 22.16881661971367 28.299889619268853 27 27.50800397590295 19.497712757486394 23.636715596632563 29.357567669978092 28 27.79953815823435 21.98642756333163 22.269724377666847 27.944309900767173 29 29.84590630268483 20.80079572974843 22.85073340033059 26.502564567236142 30 28.106448760825277 21.13379133099392 23.724237631591954 27.035522276588853 31 29.034182331394803 21.992262365662256 22.889654964112534 26.0839003388304 32 31.01293541354333 21.192345288500086 22.90482545017216 24.88989384778442 33 32.38548685590647 19.74435328423862 22.92692905429524 24.943230805559676 34 27.775443856845527 22.213023827959823 24.818091456751073 25.193440858443577 35 28.837309236286156 23.499220195829697 25.7827558938368 21.88071467404735 36 28.333250959653373 23.064012586698297 22.894460095443637 25.708276358204696 37 28.452761440331255 23.470938565709485 22.697518355601684 25.378781638357577 38 29.871579432939587 23.677078699813837 22.467970367441527 23.98337149980505 39 29.050657067387164 23.369481649889895 22.65104587115658 24.92881541156636 40 28.002589279340135 23.648522490760417 22.556796652333645 25.792091577565802 41 27.634928087777393 22.093581992015245 24.051810298906627 26.219679621300735 42 25.262634749611472 21.707386722461106 24.192120133774857 28.837858394152565 43 27.391101995090526 20.694053169464627 25.130562282739422 26.784282552705424 44 26.1718342421896 22.63566945089705 24.82646611421385 26.366030192699498 45 26.470232897851144 21.272041823863105 25.70216697694086 26.555558301344888 46 28.25952651608758 22.32395371697502 24.605498717716383 24.81102104922102 47 27.250792160222296 22.572653585726286 23.615847597708914 26.560706656342497 48 27.993940042944143 22.40268922607182 25.207032515637266 24.396338215346766 49 27.091124510563052 21.912016672432824 24.43169025299703 26.565168564007095 50 24.920166175170376 22.890066832512343 25.67690571508592 26.512861277231366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 2.0 7 6.5 8 11.0 9 77.0 10 143.0 11 546.5 12 950.0 13 1482.0 14 2014.0 15 2750.0 16 3486.0 17 2939.5 18 2393.0 19 3384.5 20 4376.0 21 3979.5 22 3583.0 23 4259.0 24 4935.0 25 5493.5 26 6052.0 27 6600.5 28 7149.0 29 8559.0 30 9969.0 31 12293.5 32 14618.0 33 16059.0 34 17500.0 35 19582.0 36 21664.0 37 24889.5 38 28115.0 39 27087.5 40 26060.0 41 29447.0 42 32834.0 43 36737.0 44 40640.0 45 48973.5 46 57307.0 47 53769.0 48 50231.0 49 53819.0 50 57407.0 51 60992.5 52 64578.0 53 69462.5 54 74347.0 55 80152.0 56 85957.0 57 106176.5 58 126396.0 59 132012.5 60 137629.0 61 133825.5 62 130022.0 63 121923.0 64 113824.0 65 106491.0 66 99158.0 67 86478.5 68 73799.0 69 63053.0 70 52307.0 71 46562.5 72 40818.0 73 32858.5 74 24899.0 75 26258.5 76 27618.0 77 17992.0 78 8366.0 79 5845.0 80 3324.0 81 2531.0 82 1738.0 83 1099.5 84 461.0 85 262.5 86 64.0 87 44.0 88 24.0 89 16.0 90 8.0 91 4.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1456776.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.345136402098722 #Duplication Level Percentage of deduplicated Percentage of total 1 68.05877846439134 5.679597896455647 2 11.119342584650825 1.855848611411522 3 4.7151488998551265 1.180456821764903 4 2.52011464117539 0.8412280171813882 5 1.4723837167663132 0.6143621476321988 6 0.9048485534690898 0.4530650761164764 7 0.5864945196371053 0.34260637358185075 8 0.398062933511368 0.2657511581417536 9 0.3310106770066891 0.24860963251546772 >10 3.643660312367656 8.13717690309877 >50 2.9137399613500246 17.924808467274 >100 3.162422107774597 47.40563340681527 >500 0.12476544547580938 7.057673764434827 >1k 0.04583220446050141 6.270906471117195 >5k 0.003394978108185289 1.7222752524587257 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 6815 0.4678138574496011 No Hit GACACTGGGATCCAAATCAAATGTAAGAAGTCCCTCACATCCGATTAGCC 6522 0.4477009505922668 No Hit GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 5872 0.40308187394630335 No Hit GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 5107 0.35056865297066947 No Hit GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 4297 0.2949664189964689 No Hit GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 3613 0.2480134214182551 No Hit GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 2938 0.20167822643975464 No Hit GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 2425 0.16646347825609428 No Hit GACACTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCT 2366 0.16241343899130684 No Hit GACACTGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGC 2316 0.15898120232623272 No Hit GACACTGGGGCTGACACTGGGGAATCTCTCTTCATCTCTAACCATGCCTA 2254 0.15472522886154083 No Hit GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT 2198 0.15088112379665783 No Hit GACACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC 2194 0.15060654486345187 No Hit GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG 2030 0.13934880860200882 No Hit GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT 1956 0.13426909833769912 No Hit GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC 1926 0.13220975633865467 No Hit GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG 1890 0.12973854593980133 No Hit GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 1885 0.12939532227329392 No Hit GACACTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA 1856 0.12740462500755093 No Hit GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 1789 0.12280542787635161 No Hit GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC 1740 0.11944183594457898 No Hit GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC 1735 0.11909861227807157 No Hit GACACTGGGACAGTGCTGGGACCATGGTTTGTTGGTGAGATAATTGATGG 1715 0.11772571761204194 No Hit GACACTGGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCC 1700 0.11669604661251969 No Hit GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGA 1668 0.11449941514687226 No Hit GACACTGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG 1664 0.11422483621366633 No Hit GACACTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA 1652 0.11340109941404856 No Hit GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 1651 0.11333245468074708 No Hit GACACTGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT 1562 0.10722307341691517 No Hit GACACTGGGGCACTAATTAGACTGATGATCATTGTGTGGACATCTCCTGG 1517 0.10413406041834847 No Hit GACACTGGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG 1485 0.10193742895270104 No Hit GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG 1462 0.10035860008676693 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.3728946660296435E-4 0.0 0.0 0.0 0.0 8 2.0593419990444652E-4 0.0 0.0 0.0 0.0 9 4.805131331103752E-4 0.0 0.0 0.0 0.0 10 0.003912749798184484 0.0 0.0 0.0 0.0 11 0.008580591662685272 0.0 0.0 0.0 0.0 12 0.01297385459398013 0.0 0.0 0.0 0.0 13 0.01764169645848092 0.0 0.0 0.0 0.0 14 0.0277324722537988 0.0 0.0 0.0 0.0 15 0.03960801111495522 0.0 0.0 0.0 0.0 16 0.04791402384443456 0.0 0.0 0.0 0.0 17 0.05368018144175906 0.0 0.0 0.0 0.0 18 0.05958362850568653 0.0 0.0 0.0 0.0 19 0.0670659044355481 0.0 0.0 0.0 0.0 20 0.07482275929861558 0.0 0.0 0.0 0.0 21 0.08038298269603562 0.0 0.0 0.0 0.0 22 0.08820848229240459 0.0 0.0 0.0 0.0 23 0.09617127135537654 0.0 0.0 0.0 0.0 24 0.10626204715069441 0.0 0.0 0.0 0.0 25 0.11223413894792336 0.0 0.0 0.0 0.0 26 0.11999099381099085 0.0 0.0 0.0 0.0 27 0.12726733554094796 0.0 0.0 0.0 0.0 28 0.14655650559866445 0.0 0.0 0.0 0.0 29 0.15273453159579783 0.0 0.0 0.0 0.0 30 0.16303124159102017 0.0 0.0 0.0 0.0 31 0.17147454378710247 0.0 0.0 0.0 0.0 32 0.1814280301158174 0.0 0.0 0.0 0.0 33 0.1871255429798404 0.0 0.0 0.0 0.0 34 0.19632393724223904 0.0 0.0 0.0 0.0 35 0.22124197543067706 0.0 0.0 0.0 0.0 36 0.23284293535862755 0.0 0.0 0.0 0.0 37 0.24080572442159948 0.0 0.0 0.0 0.0 38 0.25048463181710845 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCATC 20 7.857175E-4 44.000004 44 CTATACG 20 7.857175E-4 44.000004 26 GACAACG 20 7.857175E-4 44.000004 26 ACCGATA 20 7.857175E-4 44.000004 22 ATTGTCG 20 7.857175E-4 44.000004 42 TACGGAC 20 7.857175E-4 44.000004 28 TGCGAAA 20 7.857175E-4 44.000004 27 TCGTCAA 20 7.857175E-4 44.000004 43 CGTACTT 20 7.857175E-4 44.000004 20 CGCAATT 20 7.857175E-4 44.000004 31 ATACGGT 20 7.857175E-4 44.000004 31 GATTACG 40 8.314601E-9 44.000004 23 TATCGCC 20 7.857175E-4 44.000004 41 GCGTACG 20 7.857175E-4 44.000004 42 TAATCGT 20 7.857175E-4 44.000004 21 TCACGAA 30 2.5281843E-6 44.0 18 CCTTACG 25 4.443428E-5 44.0 18 TCGCAAT 35 1.4464422E-7 44.0 38 ACACGCA 25 4.443428E-5 44.0 11 CATATCG 30 2.5281843E-6 44.0 23 >>END_MODULE