FastQCFastQC Report
Fri 17 Jun 2016
SRR1527251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565716
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT62930.4019247424181653No Hit
GACACTGGGATCCAAATCAAATGTAAGAAGTCCCTCACATCCGATTAGCCCA59350.37905980394911976No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG57330.3661583582207757No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA54760.34974414261590225No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC40360.2577734403940434No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC33920.21664209856704536No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC27000.1724450666659854No Hit
GACACTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG24080.15379545204877512No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23650.1510491046907613No Hit
GACACTGGGGCTGACACTGGGGAATCTCTCTTCATCTCTAACCATGCCTACT23180.14804728315990895No Hit
GACACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG21100.1347626261723071No Hit
GACACTGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA20750.13252722715997026No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC20220.12914219436986019No Hit
GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT19850.12677905827110408No Hit
GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA19120.12211665461680152No Hit
GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC18970.12115862646865715No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18460.11790133076496631No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT18050.11528272049337172No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC17930.11451629797485624No Hit
GACACTGGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCAC17650.11272797876498676No Hit
GACACTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG17290.11042871120944028No Hit
GACACTGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA16620.10614951881439547No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT16320.10423346251810672No Hit
GACACTGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG16200.10346703999959124No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT16170.10327543436996238No Hit
GACACTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT16120.10295609165391424No Hit
GACACTGGGGCACTAATTAGACTGATGATCATTGTGTGGACATCTCCTGGCA15920.10167872078972176No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGAAG15920.10167872078972176No Hit
GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT15890.1014871151600929No Hit
GACACTGGGACAGTGCTGGGACCATGGTTTGTTGGTGAGATAATTGATGGCA15670.10008200720948116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTT351.0198892E-746.00000414
ATAGACC351.0198892E-746.00000421
TTACGCG700.046.00000421
CGCACTA351.0198892E-746.00000438
AACGTAC405.6134013E-946.023
CGAACTA206.312158E-446.029
TTAATCG301.8618393E-646.020
CGTATAA253.4177276E-546.035
GCGAACT206.312158E-446.029
CCGGATA453.110472E-1046.031
ATTCGAC206.312158E-446.042
CGTCTAA301.8618393E-646.030
CGCGATA253.4177276E-546.010
CGATTAT206.312158E-446.027
TAACGGT253.4177276E-546.032
ATCGTTG301.8618393E-646.023
CGTTACG206.312158E-446.046
AAACGTT206.312158E-446.044
CTAACGA206.312158E-446.040
TAACACG206.312158E-446.023
Produced by FastQC (version 0.11.5)