##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1565716
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.555370833535584	34.0	31.0	34.0	31.0	34.0
2	32.63027394495553	34.0	31.0	34.0	31.0	34.0
3	32.94068272917949	34.0	31.0	34.0	31.0	34.0
4	36.31479272102987	37.0	37.0	37.0	35.0	37.0
5	36.42230327850006	37.0	37.0	37.0	35.0	37.0
6	36.324005758387855	37.0	37.0	37.0	35.0	37.0
7	36.50801422480195	37.0	37.0	37.0	35.0	37.0
8	36.62538864008543	37.0	37.0	37.0	35.0	37.0
9	38.73224071287513	39.0	39.0	39.0	39.0	39.0
10	38.163346992685774	39.0	39.0	39.0	37.0	39.0
11	37.87093125445483	39.0	38.0	39.0	35.0	39.0
12	37.477564896826756	39.0	37.0	39.0	35.0	39.0
13	37.35456494025736	39.0	37.0	39.0	35.0	39.0
14	38.190506452000236	40.0	38.0	40.0	35.0	40.0
15	38.119382442281996	40.0	38.0	40.0	34.0	40.0
16	33.089068515618415	40.0	35.0	40.0	3.0	40.0
17	34.81934463210442	40.0	35.0	40.0	17.0	40.0
18	36.88655924829279	40.0	35.0	40.0	31.0	40.0
19	37.72465696205442	40.0	37.0	40.0	34.0	40.0
20	37.9674212947942	40.0	38.0	40.0	34.0	40.0
21	38.12294119750964	40.0	38.0	40.0	34.0	40.0
22	38.148995731026574	40.0	38.0	40.0	34.0	40.0
23	38.09840929006282	40.0	38.0	40.0	34.0	40.0
24	38.0604253900452	40.0	38.0	40.0	34.0	40.0
25	37.98620758809388	40.0	38.0	40.0	34.0	40.0
26	37.89601626348584	40.0	38.0	40.0	34.0	40.0
27	37.81520211839184	40.0	37.0	40.0	34.0	40.0
28	37.71598808468458	40.0	37.0	40.0	33.0	40.0
29	37.509189405997	40.0	37.0	40.0	33.0	40.0
30	37.46488635231421	40.0	37.0	40.0	33.0	40.0
31	37.402702022589025	40.0	36.0	40.0	33.0	40.0
32	37.32384672571526	40.0	36.0	40.0	33.0	40.0
33	37.04146090350996	40.0	36.0	40.0	32.0	40.0
34	36.92232435511932	39.0	35.0	40.0	31.0	40.0
35	36.87884328958764	39.0	35.0	40.0	31.0	40.0
36	36.785221585523814	39.0	35.0	40.0	31.0	40.0
37	36.46324620812459	39.0	35.0	40.0	31.0	40.0
38	36.47396654310233	39.0	35.0	40.0	31.0	40.0
39	36.071193626430336	39.0	35.0	40.0	30.0	40.0
40	36.018234469086345	38.0	35.0	40.0	30.0	40.0
41	35.958254881472755	38.0	35.0	40.0	30.0	40.0
42	35.91101898428578	38.0	35.0	40.0	30.0	40.0
43	35.78458353877715	38.0	35.0	40.0	30.0	40.0
44	36.03889849755639	38.0	35.0	40.0	30.0	40.0
45	36.09238840249445	38.0	35.0	40.0	30.0	40.0
46	36.032341114225055	38.0	35.0	40.0	30.0	40.0
47	35.764593323437964	38.0	35.0	40.0	30.0	40.0
48	35.67914040605065	37.0	35.0	40.0	30.0	40.0
49	35.56126206796124	37.0	35.0	40.0	30.0	40.0
50	35.072613424145885	37.0	34.0	40.0	28.0	40.0
51	35.16475082326552	36.0	34.0	40.0	29.0	40.0
52	34.81278852614395	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	4.0
15	17.0
16	22.0
17	79.0
18	167.0
19	358.0
20	646.0
21	1207.0
22	1951.0
23	3062.0
24	4445.0
25	6385.0
26	8760.0
27	11809.0
28	15677.0
29	19954.0
30	26195.0
31	33442.0
32	44318.0
33	63742.0
34	91376.0
35	120591.0
36	173101.0
37	300345.0
38	404398.0
39	233663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.5672906197548	0.38749045165279017	0.032764562666537225	0.012454365925876723
8	99.49652427387853	0.3968152589613953	0.06412401738246272	0.04253644977760973
9	98.90216361076976	0.4917239141708969	0.18937023061653582	0.416742244442798
10	54.68552406694446	28.3300419744066	5.858789205705249	11.125644752943701
11	33.37048353596693	24.722235705581344	21.64702921858115	20.260251539870577
12	29.30544236630398	16.104964118652425	28.688408370355802	25.9011851446878
13	22.787465926132196	17.15279143854952	30.883506331927375	29.17623630339091
14	23.53319503664777	19.79599109927982	32.02132442920683	24.649489434865583
15	25.272527073875466	20.459202051968557	28.00137445104987	26.266896423106108
16	26.66581934399342	29.781007539042843	21.817622097494056	21.735551019469685
17	29.76593456284537	19.475690355083554	22.64919053008336	28.109184551987713
18	27.533601240582584	20.050571112513378	24.7498269162479	27.666000730656133
19	28.611127433072152	19.254321984318995	24.158340337583574	27.97621024502528
20	29.431199527883727	20.387860889203406	24.489498734125473	25.69144084878739
21	32.43033857992126	18.587725998840146	25.110556448295863	23.87137897294273
22	31.110814477210425	18.62783544397579	24.86025562745734	25.40109445135644
23	29.827823181215496	22.41843348346699	23.160969166822078	24.592774168495435
24	28.840926451540383	21.876636631419746	23.38981015714216	25.89262675989771
25	27.18954139831234	22.315094180553814	23.212958160994717	27.28240626013913
26	27.204614374509813	21.969182150530493	22.28239348643049	28.543809988529212
27	27.493747269619778	19.45103709740464	23.566726021832825	29.48848961114276
28	27.94350955090195	21.824264425987856	22.206773131270293	28.025452891839898
29	30.115678705461274	20.682231004856565	22.767730546280422	26.434359743401743
30	28.140160795444384	21.001765326534315	23.64694491210411	27.21112896591719
31	29.109238201564015	21.926454095123255	22.74282181442867	26.22148588888406
32	30.944500790692565	20.831619527423875	23.061845187760742	25.162034494122814
33	32.705484264068325	19.590525995774456	22.805476855317313	24.898512884839906
34	27.87038006892693	22.13607065393724	24.778056812346556	25.215492464789275
35	28.79832613321956	23.538559994277378	25.739469993281027	21.92364387922203
36	28.374749954653332	22.995358034279526	23.011325170081932	25.618566840985213
37	28.795643654404756	23.33418065600658	22.621599319416806	25.24857637017186
38	29.919666146350938	23.540220576400827	22.48779472139264	24.052318555855596
39	29.368927698254343	23.203888827858947	22.553323846725714	24.873859627160993
40	27.996328836136307	23.66712737175835	22.556836616602244	25.77970717550309
41	27.657186871693206	22.05278607359189	23.994581392794096	26.29544566192081
42	25.336651091257927	21.64153652386512	24.081314874472763	28.940497510404185
43	27.33892992087965	20.64020550342463	24.965063906864334	27.055800668831388
44	26.308347107649155	22.522986288701144	24.798111534914376	26.370555068735325
45	26.54089247347539	21.054903954484722	25.582608851158188	26.82159472088169
46	28.06645649658048	22.216417281294948	24.70895104859374	25.008175173530834
47	27.490937053718557	22.51129834529378	23.533961459166285	26.46380314182138
48	27.923838039593385	22.484792899861787	25.142107508641416	24.449261551903408
49	27.220262167596164	21.692631358432816	24.45462650953302	26.632479964437994
50	24.91460775772873	22.807839991416067	25.69374011634294	26.583812134512264
51	25.78896811426849	22.715422209391743	24.426524350520783	27.069085325818985
52	26.702671493425374	23.126288547859254	23.535749778376154	26.635290180339215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	4.5
8	8.0
9	82.0
10	156.0
11	547.5
12	939.0
13	1560.0
14	2878.5
15	3576.0
16	3060.0
17	2544.0
18	3400.5
19	4257.0
20	3877.5
21	3498.0
22	4077.0
23	4656.0
24	4686.5
25	4717.0
26	5869.0
27	7021.0
28	7425.0
29	7829.0
30	11057.5
31	14286.0
32	15047.5
33	15809.0
34	16449.5
35	17090.0
36	20960.0
37	24830.0
38	26358.0
39	28145.0
40	28404.0
41	32018.0
42	35632.0
43	40038.0
44	44444.0
45	49758.5
46	55073.0
47	55463.5
48	55854.0
49	57715.0
50	59576.0
51	60706.5
52	61837.0
53	69176.5
54	76516.0
55	86417.5
56	96319.0
57	109657.5
58	122996.0
59	137598.0
60	152200.0
61	141001.5
62	129803.0
63	127690.0
64	113956.0
65	102335.0
66	96387.5
67	90440.0
68	74266.0
69	58092.0
70	50390.0
71	42688.0
72	39416.5
73	36145.0
74	33209.0
75	30273.0
76	21373.5
77	12474.0
78	8763.5
79	5053.0
80	3435.5
81	1818.0
82	1294.0
83	770.0
84	423.5
85	77.0
86	48.5
87	20.0
88	13.5
89	5.0
90	3.0
91	2.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1565716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.219701655663947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.04647352473762	7.465131664211737
2	10.010477120393787	2.046081792025489
3	3.8781547183975236	1.189007525895844
4	1.995086296425615	0.8155674690709322
5	1.109570211647045	0.566973826452235
6	0.6919637842245434	0.4242998058779434
7	0.4425501048150231	0.31659110272246416
8	0.31507933290337053	0.25760134241104543
9	0.2726199083353345	0.2507484715723414
>10	3.123342144255986	8.59539263292915
>50	2.489903595571608	18.662521511611484
>100	2.489197440269404	45.158622752081996
>500	0.09674327640195982	6.610349670229112
>1k	0.0360139204124084	5.949733271603843
>5k	0.0028246212088163447	1.691377161304392
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6293	0.4019247424181653	No Hit
GACACTGGGATCCAAATCAAATGTAAGAAGTCCCTCACATCCGATTAGCCCA	5935	0.37905980394911976	No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5733	0.3661583582207757	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5476	0.34974414261590225	No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4036	0.2577734403940434	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3392	0.21664209856704536	No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2700	0.1724450666659854	No Hit
GACACTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG	2408	0.15379545204877512	No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2365	0.1510491046907613	No Hit
GACACTGGGGCTGACACTGGGGAATCTCTCTTCATCTCTAACCATGCCTACT	2318	0.14804728315990895	No Hit
GACACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2110	0.1347626261723071	No Hit
GACACTGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA	2075	0.13252722715997026	No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2022	0.12914219436986019	No Hit
GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1985	0.12677905827110408	No Hit
GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1912	0.12211665461680152	No Hit
GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1897	0.12115862646865715	No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1846	0.11790133076496631	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1805	0.11528272049337172	No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1793	0.11451629797485624	No Hit
GACACTGGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCAC	1765	0.11272797876498676	No Hit
GACACTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1729	0.11042871120944028	No Hit
GACACTGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA	1662	0.10614951881439547	No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1632	0.10423346251810672	No Hit
GACACTGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG	1620	0.10346703999959124	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1617	0.10327543436996238	No Hit
GACACTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	1612	0.10295609165391424	No Hit
GACACTGGGGCACTAATTAGACTGATGATCATTGTGTGGACATCTCCTGGCA	1592	0.10167872078972176	No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGAAG	1592	0.10167872078972176	No Hit
GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	1589	0.1014871151600929	No Hit
GACACTGGGACAGTGCTGGGACCATGGTTTGTTGGTGAGATAATTGATGGCA	1567	0.10008200720948116	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.193427160481211E-4	0.0	0.0	0.0	0.0
8	5.109483456769938E-4	0.0	0.0	0.0	0.0
9	7.664225185154906E-4	0.0	0.0	0.0	0.0
10	0.0035766384197389563	0.0	0.0	0.0	0.0
11	0.007025539753058665	0.0	0.0	0.0	0.0
12	0.01060217817279762	0.0	0.0	0.0	0.0
13	0.014881370567842445	0.0	0.0	0.0	0.0
14	0.025036468938172695	0.0	0.0	0.0	0.0
15	0.036852149431953175	0.0	0.0	0.0	0.0
16	0.04726272197512193	0.0	0.0	0.0	0.0
17	0.05301089086398811	0.0	0.0	0.0	0.0
18	0.058822928296063906	0.0	0.0	0.0	0.0
19	0.06687036474047656	0.0	0.0	0.0	0.0
20	0.0738320359503256	0.0	0.0	0.0	0.0
21	0.07938859920956291	0.0	0.0	0.0	0.0
22	0.08737216711076594	0.0	0.0	0.0	0.0
23	0.0970163171354192	0.0	0.0	0.0	0.0
24	0.10723528404895907	0.0	0.0	0.0	0.0
25	0.11374987545634074	0.0	0.0	0.0	0.0
26	0.12230826024643039	0.0	0.0	0.0	0.0
27	0.12939766854269869	0.0	0.0	0.0	0.0
28	0.1468976493821357	0.0	0.0	0.0	0.0
29	0.15341224078951737	0.0	0.0	0.0	0.0
30	0.16280091664133214	0.0	0.0	0.0	0.0
31	0.16950711367834267	0.0	0.0	0.0	0.0
32	0.1803008974807692	0.0	0.0	0.0	0.0
33	0.18598519782642572	0.0	0.0	0.0	0.0
34	0.1942881084436769	0.0	0.0	0.0	0.0
35	0.21823881214728597	0.0	0.0	0.0	0.0
36	0.2305015724435338	0.0	0.0	0.0	0.0
37	0.23842127180152722	0.0	0.0	0.0	0.0
38	0.24563841718421475	0.0	0.0	0.0	0.0
39	0.25240848276443495	0.0	0.0	0.0	0.0
40	0.2604559192088476	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTT	35	1.0198892E-7	46.000004	14
ATAGACC	35	1.0198892E-7	46.000004	21
TTACGCG	70	0.0	46.000004	21
CGCACTA	35	1.0198892E-7	46.000004	38
AACGTAC	40	5.6134013E-9	46.0	23
CGAACTA	20	6.312158E-4	46.0	29
TTAATCG	30	1.8618393E-6	46.0	20
CGTATAA	25	3.4177276E-5	46.0	35
GCGAACT	20	6.312158E-4	46.0	29
CCGGATA	45	3.110472E-10	46.0	31
ATTCGAC	20	6.312158E-4	46.0	42
CGTCTAA	30	1.8618393E-6	46.0	30
CGCGATA	25	3.4177276E-5	46.0	10
CGATTAT	20	6.312158E-4	46.0	27
TAACGGT	25	3.4177276E-5	46.0	32
ATCGTTG	30	1.8618393E-6	46.0	23
CGTTACG	20	6.312158E-4	46.0	46
AAACGTT	20	6.312158E-4	46.0	44
CTAACGA	20	6.312158E-4	46.0	40
TAACACG	20	6.312158E-4	46.0	23
>>END_MODULE