FastQCFastQC Report
Fri 17 Jun 2016
SRR1527250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516940
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT65780.4336361359051776No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG55630.36672511767110105No Hit
GACACTGGGATCCAAATCAAATGTAAGAAGTCCCTCACATCCGATTAGCCCA53740.3542658246206178No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA52280.3446411855445832No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC40300.2656664073727372No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC31480.2075230398038156No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC25660.16915632787058157No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23590.1555104354819571No Hit
GACACTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG21630.14258968713330786No Hit
GACACTGGGGCTGACACTGGGGAATCTCTCTTCATCTCTAACCATGCCTACT20910.1378432897807428No Hit
GACACTGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA20210.13322873679908237No Hit
GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT19730.13006447189737233No Hit
GACACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG19400.1278890397774467No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC19090.12584545202842565No Hit
GACACTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG18780.1238018642794046No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT18310.12070352156314686No Hit
GACACTGGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCAC17960.11839624507231665No Hit
GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC17570.11582527983967725No Hit
GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA17540.11562751328332037No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC17520.11549566891241579No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC17010.11213363745434887No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT16020.10560734109457198No Hit
GACACTGGGACAGTGCTGGGACCATGGTTTGTTGGTGAGATAATTGATGGCA15800.10415705301462155No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT15800.10415705301462155No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGAAG15470.1019816208946959No Hit
GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT15280.10072909937110235No Hit
GACACTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT15250.10053133281474548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCACG206.312096E-446.019
CGCGATA206.312096E-446.010
CGATTAT206.312096E-446.027
TCGATTA206.312096E-446.045
CACGAAC206.312096E-446.029
ATATCGC206.312096E-446.013
CTAGTCG405.6134013E-946.040
CGCTAAT253.417677E-546.019
CGTAAAT206.312096E-446.020
CCGCGTA206.312096E-446.032
ACGTACT206.312096E-446.024
CACGTCA405.6134013E-946.027
AGGATAC253.417677E-546.046
TGTACGC206.312096E-446.031
TCGACGG206.312096E-446.021
TAATCGT206.312096E-446.021
CCGGATA301.8618011E-645.99999631
CATCGAA301.8618011E-645.99999644
ATACGGT301.8618011E-645.99999631
ATCGAAC301.8618011E-645.99999645
Produced by FastQC (version 0.11.5)