##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1516940
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53441533613722	34.0	31.0	34.0	31.0	34.0
2	32.63796920115496	34.0	31.0	34.0	31.0	34.0
3	32.925923899429115	34.0	31.0	34.0	31.0	34.0
4	36.31676994475721	37.0	37.0	37.0	35.0	37.0
5	36.43414505517687	37.0	37.0	37.0	35.0	37.0
6	36.313891122918506	37.0	37.0	37.0	35.0	37.0
7	36.50798449510198	37.0	37.0	37.0	35.0	37.0
8	36.648021015992725	37.0	37.0	37.0	35.0	37.0
9	38.675016150935434	39.0	39.0	39.0	39.0	39.0
10	38.15559415665748	39.0	39.0	39.0	37.0	39.0
11	37.80143710364286	39.0	38.0	39.0	35.0	39.0
12	37.43834429839018	39.0	37.0	39.0	35.0	39.0
13	37.255194008991786	39.0	37.0	39.0	34.0	39.0
14	38.118962516645354	40.0	38.0	40.0	34.0	40.0
15	38.22809933154904	40.0	38.0	40.0	34.0	40.0
16	38.31175590333171	40.0	38.0	40.0	35.0	40.0
17	38.288242778224586	40.0	38.0	40.0	35.0	40.0
18	38.28519519559112	40.0	38.0	40.0	35.0	40.0
19	38.228777670837346	40.0	38.0	40.0	34.0	40.0
20	38.194999143011586	40.0	38.0	40.0	34.0	40.0
21	33.9105007449207	40.0	34.0	40.0	10.0	40.0
22	34.71604743760465	40.0	34.0	40.0	17.0	40.0
23	36.73178504093768	40.0	34.0	40.0	30.0	40.0
24	37.46878518596648	40.0	37.0	40.0	33.0	40.0
25	37.70354199902435	40.0	37.0	40.0	33.0	40.0
26	37.787394359697814	40.0	37.0	40.0	34.0	40.0
27	37.77846322201274	40.0	37.0	40.0	34.0	40.0
28	37.683535274961436	40.0	37.0	40.0	33.0	40.0
29	37.64514746792886	40.0	37.0	40.0	33.0	40.0
30	37.51648384247235	40.0	37.0	40.0	33.0	40.0
31	37.36182644007014	40.0	36.0	40.0	33.0	40.0
32	37.31432357245507	40.0	36.0	40.0	33.0	40.0
33	37.045948422482105	40.0	36.0	40.0	32.0	40.0
34	36.91572046356481	39.0	35.0	40.0	31.0	40.0
35	36.86786491225757	39.0	35.0	40.0	31.0	40.0
36	36.71806267881393	39.0	35.0	40.0	31.0	40.0
37	36.61327145437526	39.0	35.0	40.0	31.0	40.0
38	36.5560780254987	39.0	35.0	40.0	31.0	40.0
39	36.37433583398157	39.0	35.0	40.0	31.0	40.0
40	31.47982978891716	37.0	30.0	40.0	9.0	40.0
41	32.3852578216673	37.0	30.0	40.0	14.0	40.0
42	34.18972141284428	37.0	31.0	40.0	24.0	40.0
43	35.018570279641914	37.0	33.0	40.0	27.0	40.0
44	35.72963663691379	38.0	34.0	40.0	30.0	40.0
45	35.96209869869606	38.0	35.0	40.0	30.0	40.0
46	35.99731960393951	38.0	35.0	40.0	30.0	40.0
47	35.97259087373265	38.0	35.0	40.0	31.0	40.0
48	35.79490355584268	38.0	35.0	40.0	30.0	40.0
49	35.61919719962556	37.0	35.0	40.0	30.0	40.0
50	35.51333473967329	37.0	35.0	40.0	30.0	40.0
51	35.35619075243582	37.0	34.0	40.0	29.0	40.0
52	34.95106859862618	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	2.0
15	12.0
16	44.0
17	93.0
18	209.0
19	377.0
20	732.0
21	1258.0
22	2203.0
23	3115.0
24	4812.0
25	6702.0
26	9229.0
27	12148.0
28	16264.0
29	20847.0
30	27583.0
31	36813.0
32	49752.0
33	62623.0
34	102998.0
35	142804.0
36	194977.0
37	200175.0
38	384529.0
39	236636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.558519124026	0.3961923345682756	0.031972259944361675	0.013316281461363006
8	99.51632892533652	0.39896106635727185	0.044431552994844886	0.04027845531135048
9	98.84042875789419	0.500547154139254	0.23402375835563702	0.4250003296109273
10	54.641646999881345	28.323928434875473	5.876962833071842	11.157461732171345
11	33.46460637863066	24.674080715123868	21.535855076667502	20.325457829577967
12	29.340053001437106	16.08824343744644	28.694477039302807	25.87722652181365
13	22.941447914881273	17.061123050351366	30.863976162537742	29.133452872229622
14	23.56830197634712	19.74138726647066	32.0377206745158	24.65259008266642
15	25.0935435811568	20.3552546574024	28.183909712974803	26.36729204846599
16	27.84764064498266	22.24530963650507	24.763932653895342	25.143117064616927
17	29.78054504462932	19.437881524648304	22.631086265771884	28.150487164950494
18	27.48948541142036	20.04944163908922	24.756285680382874	27.704787269107545
19	28.57093886376521	19.308344430234552	24.153888749719833	27.966827956280405
20	29.437156380608332	20.35485912428969	24.502880799504265	25.705103695597714
21	36.41963426371511	18.230187087162314	23.80529223304811	21.544886416074466
22	31.114612311627354	18.72763589858531	24.781929410523816	25.375822379263518
23	29.8231307764315	22.492320065394807	23.083773913272772	24.60077524490092
24	28.936015926800007	21.869355412870647	23.337508405078644	25.857120255250702
25	27.20364681529922	22.325668780571416	23.247524621936265	27.2231597821931
26	27.249264967632207	21.95281289965325	22.20213060503382	28.595791527680724
27	27.512953709441373	19.429773095837675	23.525716244544938	29.531556950176014
28	27.983572191385285	21.779503474099172	22.23561907524358	28.00130525927196
29	30.006526296359777	20.657639722072066	22.823513125107123	26.512320856461034
30	28.20249976927235	21.009334581460045	23.63429008398486	27.153875565282743
31	29.108600208314105	21.94879164634066	22.744406502564374	26.19820164278086
32	30.9606180864108	20.869184015188473	23.048373699684895	25.121824198715835
33	32.77473070787243	19.577504713436262	22.77459886350152	24.873165715189792
34	27.904201880100725	22.07476894273999	24.73736601315807	25.28366316400121
35	28.783735678405208	23.55452423958759	25.708070193943072	21.953669888064127
36	28.389455087215048	23.00341476920643	23.01778580563503	25.589344337943494
37	28.68887365353936	23.290571809036614	22.677956939628462	25.342597597795564
38	29.95022874998352	23.527232454810342	22.487903278969505	24.034635516236634
39	29.155009426872518	23.170329742771635	22.572547365090248	25.102113465265603
40	28.44212691339143	27.937888116866848	20.82073120888104	22.79925376086068
41	27.691009532348016	22.01899877384735	24.01294711722283	26.277044576581805
42	25.342795364351918	21.716547786992237	24.122641633815444	28.818015214840404
43	27.371748388202565	20.560338576344485	25.009558716890584	27.058354318562365
44	26.291745223937664	22.54004772766227	24.745935897266865	26.422271151133202
45	26.525241604809686	21.118501720569043	25.58301580814007	26.773240866481206
46	28.055361451342836	22.21083233351352	24.72484079792213	25.008965417221514
47	27.32988780044036	22.449998022334437	23.575883027674134	26.644231149551068
48	27.947776444684695	22.46707186836658	25.132437670573655	24.452714016375072
49	27.142932482497663	21.724062916133796	24.518702124012815	26.61430247735573
50	24.901973710232443	22.76082112674199	25.766740938995607	26.570464224029955
51	25.864042084723195	22.769720621778053	24.349677640513136	27.016559652985617
52	26.787018603240735	23.068084433135127	23.56507178925996	26.57982517436418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	3.0
8	4.0
9	63.5
10	123.0
11	514.0
12	905.0
13	1464.0
14	2693.5
15	3364.0
16	2882.0
17	2400.0
18	3263.5
19	4127.0
20	3790.5
21	3454.0
22	3991.5
23	4529.0
24	4444.0
25	4359.0
26	5530.5
27	6702.0
28	7024.5
29	7347.0
30	10379.0
31	13411.0
32	14131.0
33	14851.0
34	15694.0
35	16537.0
36	20058.5
37	23580.0
38	24909.5
39	26869.5
40	27500.0
41	30962.0
42	34424.0
43	38682.0
44	42940.0
45	47816.5
46	52693.0
47	53381.0
48	54069.0
49	56077.5
50	58086.0
51	59515.0
52	60944.0
53	67683.5
54	74423.0
55	83167.5
56	91912.0
57	104836.5
58	117761.0
59	133084.0
60	148407.0
61	137484.0
62	126561.0
63	124527.0
64	110767.0
65	99041.0
66	93910.5
67	88780.0
68	73013.0
69	57246.0
70	49458.5
71	41671.0
72	37961.0
73	34251.0
74	32030.5
75	29810.0
76	21115.5
77	12421.0
78	8699.0
79	4977.0
80	3365.5
81	1754.0
82	1239.5
83	725.0
84	397.0
85	69.0
86	42.0
87	15.0
88	10.0
89	4.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1516940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.123529354249479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.87132166266677	7.478384932892126
2	8.929490370821263	1.8079591577499423
3	3.6569981766300352	1.1106518516865294
4	1.9376189425317645	0.7846216896828061
5	1.1261155076032456	0.5700131698748505
6	0.6858494039689097	0.41659299442042597
7	0.46230890709854505	0.32761384542201805
8	0.3361974403218043	0.2722803724737059
9	0.26460972061066823	0.24109058466196676
>10	3.480022113554663	9.468206312063925
>50	2.6362867310206295	19.481718595483287
>100	2.478273503150578	44.1405736153429
>500	0.09540421305361621	6.3626140429179285
>1k	0.03652192530958746	5.821599815628558
>5k	0.0029813816579255066	1.7160790196990436
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6578	0.4336361359051776	No Hit
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5563	0.36672511767110105	No Hit
GACACTGGGATCCAAATCAAATGTAAGAAGTCCCTCACATCCGATTAGCCCA	5374	0.3542658246206178	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5228	0.3446411855445832	No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4030	0.2656664073727372	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3148	0.2075230398038156	No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2566	0.16915632787058157	No Hit
GACACTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2359	0.1555104354819571	No Hit
GACACTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG	2163	0.14258968713330786	No Hit
GACACTGGGGCTGACACTGGGGAATCTCTCTTCATCTCTAACCATGCCTACT	2091	0.1378432897807428	No Hit
GACACTGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA	2021	0.13322873679908237	No Hit
GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1973	0.13006447189737233	No Hit
GACACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1940	0.1278890397774467	No Hit
GACACTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1909	0.12584545202842565	No Hit
GACACTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1878	0.1238018642794046	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1831	0.12070352156314686	No Hit
GACACTGGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCAC	1796	0.11839624507231665	No Hit
GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1757	0.11582527983967725	No Hit
GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1754	0.11562751328332037	No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1752	0.11549566891241579	No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1701	0.11213363745434887	No Hit
GACACTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1602	0.10560734109457198	No Hit
GACACTGGGACAGTGCTGGGACCATGGTTTGTTGGTGAGATAATTGATGGCA	1580	0.10415705301462155	No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1580	0.10415705301462155	No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGAAG	1547	0.1019816208946959	No Hit
GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	1528	0.10072909937110235	No Hit
GACACTGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	1525	0.10053133281474548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.3184437090458422E-4	0.0	0.0	0.0	0.0
8	2.6368874180916844E-4	0.0	0.0	0.0	0.0
9	3.296109272614606E-4	0.0	0.0	0.0	0.0
10	0.0039553311271375265	0.0	0.0	0.0	0.0
11	0.007383284770656717	0.0	0.0	0.0	0.0
12	0.011602304639603413	0.0	0.0	0.0	0.0
13	0.01463472517040885	0.0	0.0	0.0	0.0
14	0.023204609279206825	0.0	0.0	0.0	0.0
15	0.03454322517700107	0.0	0.0	0.0	0.0
16	0.0442997086239403	0.0	0.0	0.0	0.0
17	0.048386884121982415	0.0	0.0	0.0	0.0
18	0.05306735928909515	0.0	0.0	0.0	0.0
19	0.05992326657613353	0.0	0.0	0.0	0.0
20	0.06710878479043338	0.0	0.0	0.0	0.0
21	0.0717892599575461	0.0	0.0	0.0	0.0
22	0.08068875499360555	0.0	0.0	0.0	0.0
23	0.08761058446609622	0.0	0.0	0.0	0.0
24	0.09835590069481984	0.0	0.0	0.0	0.0
25	0.10409113082916925	0.0	0.0	0.0	0.0
26	0.11094703811620762	0.0	0.0	0.0	0.0
27	0.11885770037048268	0.0	0.0	0.0	0.0
28	0.13659076825714925	0.0	0.0	0.0	0.0
29	0.14272153150421243	0.0	0.0	0.0	0.0
30	0.15089588250029665	0.0	0.0	0.0	0.0
31	0.1595316887945469	0.0	0.0	0.0	0.0
32	0.17001331628146135	0.0	0.0	0.0	0.0
33	0.1755507798594539	0.0	0.0	0.0	0.0
34	0.18187930966287394	0.0	0.0	0.0	0.0
35	0.20126043218584783	0.0	0.0	0.0	0.0
36	0.21200574841457143	0.0	0.0	0.0	0.0
37	0.21866388914525295	0.0	0.0	0.0	0.0
38	0.22551979643229134	0.0	0.0	0.0	0.0
39	0.23270531464659117	0.0	0.0	0.0	0.0
40	0.24226403153717352	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCACG	20	6.312096E-4	46.0	19
CGCGATA	20	6.312096E-4	46.0	10
CGATTAT	20	6.312096E-4	46.0	27
TCGATTA	20	6.312096E-4	46.0	45
CACGAAC	20	6.312096E-4	46.0	29
ATATCGC	20	6.312096E-4	46.0	13
CTAGTCG	40	5.6134013E-9	46.0	40
CGCTAAT	25	3.417677E-5	46.0	19
CGTAAAT	20	6.312096E-4	46.0	20
CCGCGTA	20	6.312096E-4	46.0	32
ACGTACT	20	6.312096E-4	46.0	24
CACGTCA	40	5.6134013E-9	46.0	27
AGGATAC	25	3.417677E-5	46.0	46
TGTACGC	20	6.312096E-4	46.0	31
TCGACGG	20	6.312096E-4	46.0	21
TAATCGT	20	6.312096E-4	46.0	21
CCGGATA	30	1.8618011E-6	45.999996	31
CATCGAA	30	1.8618011E-6	45.999996	44
ATACGGT	30	1.8618011E-6	45.999996	31
ATCGAAC	30	1.8618011E-6	45.999996	45
>>END_MODULE