FastQCFastQC Report
Fri 17 Jun 2016
SRR1527249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148323
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC187460.8725875950683394No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC92520.4306614973632922No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG67050.31210390616308625No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT50930.23706863446511534No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG37100.17269283995004475No Hit
TGGATGGAGATTTCTAAAGAGACTTCTGGGCTGTTAAACTATAAAAGATG35390.1647331430143419No Hit
TGGATGGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAG31980.14886029707823267No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT31910.1485344615311571No Hit
TGGATGGGGGGGGCTGGTGCGATGGCTCAGCGTGTAAGAACACCTGACTG31830.14816207804878503No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT29740.13843355957181486No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT29530.13745605293058819No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTG27100.12614490465353673No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGC25680.11953509784143258No Hit
TGGATGGGGGGGCTGGCAAGATGGCTCAGCGGTTAAGAGCATTGTCTGCT24780.11534578366474689No Hit
TGGATGGGGATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACA22960.1068740594407824No Hit
TGGATGGGGACTGGAGAGTGAGAGAGCTGTTCACAGTTCATCTGTGTCTA22820.10622238834663131No Hit
TGGATGGGGGGTAGCTTAACAACCTCTGGATTGATTGTGTATAATTGTCT21630.10068318404634685No Hit
TGGATGGGGGACTTAGGCTCCAACGTAACTGCAATTTAATGTTGGTAGTA21520.10017115675808524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT207.85801E-444.00000416
AGGTACG207.85801E-444.00000422
GTTTCGC408.3182385E-944.00000431
ACGTTAT207.85801E-444.00000440
GCGCATT207.85801E-444.00000432
GTCACGC207.85801E-444.00000420
TCGTCTA207.85801E-444.00000422
GCGATAA207.85801E-444.00000433
CCGCGTA408.3182385E-944.00000438
GACGATA207.85801E-444.0000049
CGCGTAT408.3182385E-944.00000439
ATTGCGA207.85801E-444.00000444
CGATACG207.85801E-444.00000436
ATACCGG207.85801E-444.00000421
GCGTAGC207.85801E-444.00000444
TACGTCG207.85801E-444.00000432
TACGTAA800.044.00000442
TACCGGC207.85801E-444.00000422
CGACGTT254.4441367E-544.017
CGTGATA254.4441367E-544.018
Produced by FastQC (version 0.11.5)