##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527249_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2148323
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38605647288606	33.0	31.0	34.0	31.0	34.0
2	32.76738367554599	34.0	31.0	34.0	31.0	34.0
3	33.17718750858228	34.0	34.0	34.0	31.0	34.0
4	35.38908022676292	35.0	35.0	37.0	33.0	37.0
5	36.01076793387214	37.0	35.0	37.0	35.0	37.0
6	36.23288350960261	37.0	37.0	37.0	35.0	37.0
7	36.61770646220331	37.0	37.0	37.0	35.0	37.0
8	36.69227485811026	37.0	37.0	37.0	35.0	37.0
9	38.65223804800302	39.0	39.0	39.0	38.0	39.0
10	38.07500734293679	39.0	38.0	39.0	37.0	39.0
11	37.25743940738893	39.0	35.0	39.0	35.0	39.0
12	37.10770214720971	39.0	37.0	39.0	34.0	39.0
13	36.96069631987369	39.0	35.0	39.0	33.0	39.0
14	37.71374648970383	40.0	37.0	40.0	33.0	40.0
15	37.74501087592508	40.0	36.0	40.0	33.0	40.0
16	37.662618237574144	40.0	36.0	40.0	33.0	40.0
17	37.67525320913103	40.0	36.0	40.0	33.0	40.0
18	37.62199352704412	40.0	36.0	40.0	33.0	40.0
19	37.608790205197266	40.0	36.0	40.0	33.0	40.0
20	37.337039169622074	40.0	36.0	40.0	33.0	40.0
21	37.582296982343905	40.0	36.0	40.0	33.0	40.0
22	37.45660219622469	40.0	36.0	40.0	33.0	40.0
23	37.608135741226995	40.0	36.0	40.0	34.0	40.0
24	37.647327706308594	40.0	36.0	40.0	34.0	40.0
25	37.62081865715724	40.0	36.0	40.0	34.0	40.0
26	37.52774326765575	40.0	36.0	40.0	34.0	40.0
27	37.41616833222937	40.0	35.0	40.0	33.0	40.0
28	37.307642752044266	40.0	35.0	40.0	33.0	40.0
29	37.53662740658644	40.0	35.0	40.0	34.0	40.0
30	37.60227908000799	40.0	35.0	40.0	34.0	40.0
31	37.56082907458515	40.0	35.0	40.0	34.0	40.0
32	36.83895159154373	40.0	35.0	40.0	32.0	40.0
33	37.056661870677736	40.0	35.0	40.0	33.0	40.0
34	37.02357652922768	40.0	35.0	40.0	32.0	40.0
35	37.06832399038692	40.0	35.0	40.0	33.0	40.0
36	36.981614496516585	40.0	35.0	40.0	33.0	40.0
37	36.97449312789557	40.0	35.0	40.0	33.0	40.0
38	36.81139661028626	40.0	35.0	40.0	32.0	40.0
39	36.784363431383454	40.0	35.0	40.0	32.0	40.0
40	36.74785076545752	39.0	35.0	40.0	32.0	40.0
41	36.62468213578684	39.0	35.0	40.0	32.0	40.0
42	36.55677195654471	39.0	35.0	40.0	32.0	40.0
43	36.4944154114628	39.0	35.0	40.0	31.0	40.0
44	36.389703503616545	39.0	35.0	40.0	31.0	40.0
45	36.30250479094624	39.0	35.0	40.0	31.0	40.0
46	36.03464562824119	39.0	35.0	40.0	30.0	40.0
47	36.0015854226762	38.0	35.0	40.0	30.0	40.0
48	35.924287921322815	38.0	35.0	40.0	30.0	40.0
49	35.68462703234104	38.0	35.0	40.0	29.0	40.0
50	35.27568899090127	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	8.0
15	26.0
16	62.0
17	169.0
18	388.0
19	603.0
20	1054.0
21	1666.0
22	2486.0
23	3726.0
24	5293.0
25	7477.0
26	10328.0
27	14069.0
28	19357.0
29	25579.0
30	34123.0
31	43977.0
32	55872.0
33	81172.0
34	174987.0
35	162855.0
36	149886.0
37	260342.0
38	678614.0
39	414201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.83299671418125	1.0264285212232984	0.0992867459874516	0.041288018608002616
8	98.76112670208343	1.0584069527719995	0.1026847452640967	0.0777815998804649
9	93.2575781202361	3.427976146976037	1.8767662032199066	1.4376795295679468
10	40.70761240279045	43.62789021948748	5.9747998787891765	9.689697498932889
11	31.96753933184163	19.9589633402426	29.365975228119794	18.70752209979598
12	31.569694128862373	16.331250002909243	28.98358393965898	23.115471928569402
13	24.80427756906201	16.14989924699405	32.171791671922705	26.874031512021233
14	24.30919372924835	19.378603682965736	33.2968552680393	23.015347319746613
15	26.11702244029413	21.10185479557776	31.22114318936212	21.559979574765993
16	31.33662861683276	22.20732171093453	27.213691795879857	19.24235787635286
17	29.680778914530077	22.238974306936154	25.54504141137064	22.53520536716313
18	27.769799978867237	22.046498594485094	27.166492189489194	23.017209237158472
19	26.63156331706173	23.86940883656694	26.77893408021047	22.72009376616086
20	27.375445871035218	24.053971400017595	27.41505816397255	21.155524564974634
21	27.044164215529975	23.391221897265915	28.362355195191785	21.202258692012325
22	25.40204615413976	22.534786435745463	29.834899128296815	22.228268281817957
23	25.824980694243838	22.507276605985226	30.93696804437694	20.730774655394
24	26.704224644059575	23.636715708019697	27.146243837635215	22.51281581028551
25	25.506825556492203	25.199795375276434	25.2922861227106	24.001092945520764
26	25.315513542423556	24.408899406653468	25.736167233698097	24.53941981722488
27	22.930024954348113	21.862913537675666	28.93242775876812	26.274633749208103
28	23.740471055795613	24.684882115026465	26.46217538051773	25.11247144866019
29	26.782657915034193	24.812283813933007	25.378585994750324	23.02647227628248
30	26.59139244890084	22.55638467772304	27.71343042922317	23.13879244415295
31	26.562765468693488	23.36580672459402	26.485914827518954	23.58551297919354
32	29.114290541971577	23.383448392071397	26.680904128476023	20.821356937481003
33	29.957459841932522	22.515375946726817	26.766459233551004	20.76070497778965
34	24.663470064790072	24.43952794807857	30.066987133685203	20.83001485344615
35	23.098575028056768	27.891895213149976	29.31323641742885	19.696293341364406
36	24.109689278567515	29.878700735410828	25.644514349099275	20.36709563692238
37	25.65419631964095	28.37119930289812	24.892299714707704	21.082304662753227
38	25.973189320227917	27.44079917219152	25.822187818126046	20.76382368945452
39	25.74463895792206	26.66698629582237	24.525083053153555	23.063291693102013
40	24.1552131592875	25.437469132900407	26.103756278734625	24.303561429077472
41	22.296135171480266	27.78129731888547	25.424342615146788	24.498224894487468
42	22.226685652017878	25.810224998754844	26.831300507419044	25.131788841808238
43	22.21104554575825	24.992191583854012	26.900796574816727	25.89596629557101
44	23.37325439424146	24.370311168292663	25.97062918378661	26.285805253679268
45	24.723144517840197	25.38002898074452	25.747152546428076	24.149673954987215
46	24.01687269558628	27.44089226806211	24.66337696891948	23.878858067432134
47	23.213967359656813	25.496957394209346	27.24669428200508	24.042380964128764
48	24.28573356985891	26.161661910243478	26.80430270494707	22.748301814950544
49	24.822850195245312	25.512132021116006	26.147278598236856	23.517739185401823
50	22.73000847637902	25.54383116505293	26.488195676348482	25.23796468221957
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	27.0
8	54.0
9	223.0
10	392.0
11	1346.5
12	2301.0
13	3827.5
14	5354.0
15	7477.0
16	9600.0
17	9015.5
18	8431.0
19	9394.0
20	10357.0
21	10850.0
22	11343.0
23	11946.0
24	12549.0
25	14278.0
26	16007.0
27	18805.0
28	21603.0
29	22032.5
30	22462.0
31	28101.0
32	33740.0
33	38811.5
34	43883.0
35	51971.5
36	60060.0
37	61156.5
38	62253.0
39	67338.0
40	72423.0
41	79014.5
42	85606.0
43	90039.5
44	94473.0
45	96305.5
46	98138.0
47	104670.0
48	111202.0
49	114834.5
50	118467.0
51	122429.5
52	126392.0
53	131792.0
54	137192.0
55	140674.0
56	144156.0
57	162792.0
58	181428.0
59	174938.0
60	168448.0
61	156011.0
62	143574.0
63	139101.0
64	134628.0
65	109802.5
66	84977.0
67	64986.5
68	44996.0
69	36158.0
70	27320.0
71	24439.0
72	21558.0
73	19080.0
74	16602.0
75	12768.5
76	8935.0
77	6618.0
78	4301.0
79	3039.5
80	1778.0
81	1342.0
82	906.0
83	624.0
84	342.0
85	190.5
86	39.0
87	41.0
88	43.0
89	25.5
90	8.0
91	5.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2148323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.3386777441112745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.80154117106684	4.828963057203301
2	10.739802604033946	1.5763190069274446
3	4.833002240861087	1.0640353794674156
4	2.5403443708846307	0.745710747879576
5	1.5124271366883444	0.5549607683802348
6	0.9120860173442435	0.4016103213719574
7	0.6498042780659286	0.3338092935429507
8	0.4374107694984186	0.2568013343322105
9	0.32932647506334833	0.21751387857846102
>10	5.04641434627967	10.182459370992794
>50	3.663257793931951	19.53809490455657
>100	3.3322844752334095	44.79059398855018
>500	0.14525682724445	7.16619909124039
>1k	0.05438840106869817	6.405839394422845
>5k	0.0019898195512938355	1.0246188859874084
>10k+	6.632731837646117E-4	0.9124705765662686
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	18746	0.8725875950683394	No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	9252	0.4306614973632922	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	6705	0.31210390616308625	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT	5093	0.23706863446511534	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	3710	0.17269283995004475	No Hit
TGGATGGAGATTTCTAAAGAGACTTCTGGGCTGTTAAACTATAAAAGATG	3539	0.1647331430143419	No Hit
TGGATGGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAG	3198	0.14886029707823267	No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3191	0.1485344615311571	No Hit
TGGATGGGGGGGGCTGGTGCGATGGCTCAGCGTGTAAGAACACCTGACTG	3183	0.14816207804878503	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	2974	0.13843355957181486	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT	2953	0.13745605293058819	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTG	2710	0.12614490465353673	No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGC	2568	0.11953509784143258	No Hit
TGGATGGGGGGGCTGGCAAGATGGCTCAGCGGTTAAGAGCATTGTCTGCT	2478	0.11534578366474689	No Hit
TGGATGGGGATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACA	2296	0.1068740594407824	No Hit
TGGATGGGGACTGGAGAGTGAGAGAGCTGTTCACAGTTCATCTGTGTCTA	2282	0.10622238834663131	No Hit
TGGATGGGGGGTAGCTTAACAACCTCTGGATTGATTGTGTATAATTGTCT	2163	0.10068318404634685	No Hit
TGGATGGGGGACTTAGGCTCCAACGTAACTGCAATTTAATGTTGGTAGTA	2152	0.10017115675808524	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.792876117790481E-4	0.0	0.0	0.0	0.0
8	6.982190294476203E-4	0.0	0.0	0.0	0.0
9	0.002187752958935877	0.0	0.0	0.0	0.0
10	0.010240545765231765	0.0	0.0	0.0	0.0
11	0.016152133547888282	0.0	0.0	0.0	0.0
12	0.019363941083347335	0.0	0.0	0.0	0.0
13	0.024530761901259728	0.0	0.0	0.0	0.0
14	0.03677286888424133	0.0	0.0	0.0	0.0
15	0.04943390728489152	0.0	0.0	0.0	0.0
16	0.06200184981494868	0.0	0.0	0.0	0.0
17	0.0702408343624306	0.0	0.0	0.0	0.0
18	0.07903839413347062	0.0	0.0	0.0	0.0
19	0.08927893989870238	0.0	0.0	0.0	0.0
20	0.0951439797460624	0.0	0.0	0.0	0.0
21	0.1038484436465094	0.0	0.0	0.0	0.0
22	0.11106337361746814	0.0	0.0	0.0	0.0
23	0.1160440026941945	0.0	0.0	0.0	0.0
24	0.1230261929886707	0.0	0.0	0.0	0.0
25	0.1275878906477285	0.0	0.0	0.0	0.0
26	0.13303399907741992	0.0	0.0	0.0	0.0
27	0.1382008198953323	0.0	0.0	0.0	0.0
28	0.14588122921925614	0.0	0.0	0.0	0.0
29	0.15156007732543011	0.0	0.0	0.0	0.0
30	0.1585422676199063	0.0	0.0	0.0	0.0
31	0.1648727868202314	0.0	0.0	0.0	0.0
32	0.17273938788534127	0.0	0.0	0.0	0.0
33	0.17888371534448033	0.0	0.0	0.0	0.0
34	0.18484185106243334	0.0	0.0	0.0	0.0
35	0.1968512183689324	0.0	0.0	0.0	0.0
36	0.2089536815460245	0.0	0.0	0.0	0.0
37	0.21868220002299468	0.0	0.0	0.0	0.0
38	0.22622296554102897	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	7.85801E-4	44.000004	16
AGGTACG	20	7.85801E-4	44.000004	22
GTTTCGC	40	8.3182385E-9	44.000004	31
ACGTTAT	20	7.85801E-4	44.000004	40
GCGCATT	20	7.85801E-4	44.000004	32
GTCACGC	20	7.85801E-4	44.000004	20
TCGTCTA	20	7.85801E-4	44.000004	22
GCGATAA	20	7.85801E-4	44.000004	33
CCGCGTA	40	8.3182385E-9	44.000004	38
GACGATA	20	7.85801E-4	44.000004	9
CGCGTAT	40	8.3182385E-9	44.000004	39
ATTGCGA	20	7.85801E-4	44.000004	44
CGATACG	20	7.85801E-4	44.000004	36
ATACCGG	20	7.85801E-4	44.000004	21
GCGTAGC	20	7.85801E-4	44.000004	44
TACGTCG	20	7.85801E-4	44.000004	32
TACGTAA	80	0.0	44.000004	42
TACCGGC	20	7.85801E-4	44.000004	22
CGACGTT	25	4.4441367E-5	44.0	17
CGTGATA	25	4.4441367E-5	44.0	18
>>END_MODULE