FastQCFastQC Report
Fri 17 Jun 2016
SRR1527248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281837
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC179800.7879616291610664No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA96030.420845134862832No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT63680.2790733956895256No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT50740.22236470002020303No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT36360.15934529942322787No Hit
TGGATGGAGATTTCTAAAGAGACTTCTGGGCTGTTAAACTATAAAAGATGTT35960.15759232583221325No Hit
TGGATGGGGGGGGCTGGTGCGATGGCTCAGCGTGTAAGAACACCTGACTGCT30100.13191126272384926No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC29380.12875591026002295No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT29040.12726588270766054No Hit
TGGATGGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGAT28900.12665234195080544No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT28100.12314639476877622No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT27470.1203854613629282No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC24000.10517841546087649No Hit
TGGATGGGGGGGCTGGCAAGATGGCTCAGCGGTTAAGAGCATTGTCTGCTCT23770.10417045564604308No Hit
TGGATGGGGATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATG23240.10184776563794873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG206.3127687E-446.017
CGTATAG2450.046.032
TACGCCG206.3127687E-446.042
AACGCGT206.3127687E-446.018
GCGATAA206.3127687E-446.033
TAAGGCG253.418223E-546.037
CAGTACG405.6152203E-946.019
CGGTAAC206.3127687E-446.020
TCGACTA253.418223E-546.010
TGTCGCG405.6152203E-946.042
CGGACTA206.3127687E-446.031
CCGCGTA351.020162E-745.99999638
CGCGTAT351.020162E-745.99999639
CGCGTAG351.020162E-745.99999645
TACCACG3700.044.75675624
ACGTAAC1650.044.6060623
CGTAACT1600.044.562524
TGGATGG2330300.044.489891
ATGGGGA944400.044.373154
GATGGGG2153150.044.3389473
Produced by FastQC (version 0.11.5)