##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2281837
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3638445690906	33.0	31.0	34.0	31.0	34.0
2	32.73302869573944	34.0	31.0	34.0	31.0	34.0
3	33.11630979776382	34.0	34.0	34.0	31.0	34.0
4	35.294644183611716	35.0	35.0	37.0	33.0	37.0
5	35.94770047115548	37.0	35.0	37.0	35.0	37.0
6	36.23711816400558	37.0	37.0	37.0	35.0	37.0
7	36.6201577939178	37.0	37.0	37.0	35.0	37.0
8	36.65864213789153	37.0	37.0	37.0	35.0	37.0
9	38.61577141574968	39.0	39.0	39.0	38.0	39.0
10	37.982864244904434	39.0	38.0	39.0	35.0	39.0
11	37.18197837970021	39.0	35.0	39.0	34.0	39.0
12	37.06038774899346	39.0	37.0	39.0	34.0	39.0
13	36.982366838647984	39.0	35.0	39.0	33.0	39.0
14	37.72675787096099	40.0	37.0	40.0	33.0	40.0
15	37.668741018749365	40.0	36.0	40.0	33.0	40.0
16	32.674444756571134	39.0	34.0	40.0	3.0	40.0
17	34.291057599644496	39.0	34.0	40.0	17.0	40.0
18	36.304198766169534	39.0	34.0	40.0	30.0	40.0
19	37.22145403023967	39.0	35.0	40.0	32.0	40.0
20	37.470990697407395	39.0	36.0	40.0	33.0	40.0
21	37.68886384084402	40.0	36.0	40.0	33.0	40.0
22	37.74982700341874	40.0	36.0	40.0	34.0	40.0
23	37.77380724390042	40.0	36.0	40.0	34.0	40.0
24	37.73737650848855	40.0	36.0	40.0	34.0	40.0
25	37.69988785351452	40.0	36.0	40.0	34.0	40.0
26	37.62166798066645	40.0	36.0	40.0	34.0	40.0
27	37.51954324520113	40.0	35.0	40.0	34.0	40.0
28	37.45200161098273	40.0	35.0	40.0	33.0	40.0
29	37.273063325732736	40.0	35.0	40.0	33.0	40.0
30	37.30273810092483	40.0	35.0	40.0	33.0	40.0
31	37.267069909025054	40.0	35.0	40.0	33.0	40.0
32	37.19811932228288	39.0	35.0	40.0	33.0	40.0
33	36.85841057008016	39.0	35.0	40.0	32.0	40.0
34	36.731432613284824	39.0	35.0	40.0	31.0	40.0
35	36.70954980570479	39.0	35.0	40.0	31.0	40.0
36	36.77703972720225	39.0	35.0	40.0	32.0	40.0
37	36.572857745754845	39.0	35.0	40.0	31.0	40.0
38	36.59560476931525	39.0	35.0	40.0	31.0	40.0
39	36.238483292189585	38.0	35.0	40.0	31.0	40.0
40	36.26581477993388	38.0	35.0	40.0	31.0	40.0
41	36.21927245460565	38.0	35.0	40.0	31.0	40.0
42	36.20107527400073	38.0	35.0	40.0	31.0	40.0
43	36.12000243663329	38.0	35.0	40.0	31.0	40.0
44	36.37024949634878	39.0	35.0	40.0	31.0	40.0
45	36.42770758822826	39.0	35.0	40.0	31.0	40.0
46	36.36592140455256	39.0	35.0	40.0	32.0	40.0
47	36.10507236055862	38.0	35.0	40.0	31.0	40.0
48	36.09775807825011	38.0	35.0	40.0	31.0	40.0
49	36.059455605286445	38.0	35.0	40.0	31.0	40.0
50	35.60834538137474	38.0	35.0	40.0	30.0	40.0
51	35.6836592622523	37.0	35.0	40.0	30.0	40.0
52	35.35675379091495	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	11.0
15	27.0
16	82.0
17	169.0
18	279.0
19	628.0
20	988.0
21	1670.0
22	2620.0
23	4019.0
24	5905.0
25	8213.0
26	11433.0
27	16195.0
28	21855.0
29	29394.0
30	39725.0
31	53104.0
32	74091.0
33	114018.0
34	182796.0
35	182179.0
36	212837.0
37	380733.0
38	596649.0
39	342215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.84229241615418	1.0259716184810748	0.09260082994534667	0.03913513541940112
8	98.7714284587374	1.0448160845844818	0.1086843626429057	0.07507109403520058
9	93.2997405160842	3.4174658400227536	1.8574069926993033	1.4253866511937532
10	40.895909742895746	43.286133058583935	5.964974711164732	9.852982487355582
11	31.982433451644443	20.025532060353125	29.16983114920128	18.82220333880115
12	31.695033431397597	16.277148630686593	28.902371203552224	23.125446734363585
13	24.936925818978306	16.038305978910852	32.0220068304616	27.002761371649246
14	24.371986254934072	19.314394498818277	33.21043527648995	23.103183969757698
15	26.1779434727371	21.119080810767816	31.195961850035737	21.50701386645935
16	29.59308662275176	29.760451776353875	23.877034161511098	16.769427439383268
17	29.763782426176803	22.190980337333475	25.555199604529154	22.490037631960565
18	27.987844881119905	21.915018469768	27.08528260344626	23.01185404566584
19	26.633979552439545	23.89934075045676	26.663210387069714	22.80346931003398
20	27.278547941855617	24.032479094694317	27.46528345363845	21.223689509811614
21	27.023314987003893	23.435109519216315	28.296499706157803	21.24507578762199
22	25.07584897606621	22.5209337915022	30.08540925578821	22.31780797664338
23	25.912850041435913	22.44875510389217	30.884633740271543	20.75376111440037
24	26.936279848210017	23.46425270516693	27.05701590429115	22.5424515423319
25	25.671640875312303	25.05687303694348	25.08899627799882	24.182489809745395
26	25.19873242479634	24.29993904034337	25.817225332046068	24.68410320281422
27	23.061068779233572	21.72709970081123	28.811742468896774	26.400089051058423
28	23.782198290237208	24.769823611414836	26.45495712445718	24.993020973890772
29	27.085238779106486	24.71460494329788	25.209907631439055	22.990248646156584
30	26.587876346995863	22.439157573481367	27.717755475084328	23.255210604438442
31	26.65483993817262	23.238119111926046	26.41647935413441	23.69056159576692
32	29.12578768772704	23.052479208637603	26.83272293332083	20.989010170314533
33	30.211185110943507	22.46908959754794	26.626003522600435	20.693721768908123
34	24.77271601784001	24.264660446824205	30.036851887317106	20.925771648018678
35	22.959878378692256	28.01041441610422	29.332419449767883	19.697287755435642
36	24.23735788314415	29.880968710736134	25.568741325519746	20.312932080599975
37	25.927268249222006	28.287515716503854	24.80619781342839	20.97901822084575
38	25.826603740757992	27.49714374865514	25.755783607681003	20.92046890290586
39	26.028940717500852	26.580294736214725	24.362914616600573	23.027849929683846
40	24.133318900517434	25.41062310761023	26.025741540697254	24.43031645117508
41	22.211008060610816	27.865092905409107	25.406196849292918	24.517702184687163
42	22.202637611713723	25.728437219661178	26.76895851894767	25.29996664967743
43	22.22069323970117	24.948276322980124	26.838814516549604	25.9922159207691
44	23.487391956568327	24.21619072703265	25.946463310043615	26.349954006355407
45	24.7620667032746	25.32455210429141	25.69052916575549	24.222852026678503
46	23.794118510656105	27.54811145581389	24.608637689721043	24.049132343808957
47	23.339923053224222	25.44182603753029	27.27455992693606	23.94369098230943
48	24.397009952945805	26.147354083573894	26.696122466241018	22.759513497239286
49	24.763994974224715	25.381567570339158	26.216026824001894	23.638410631434233
50	22.79334588754587	25.580354775560217	26.283647780275277	25.342651556618634
51	21.500308742473717	25.959698260655777	28.082417806355142	24.45757519051536
52	21.693135837485325	25.646748650319896	28.244348741825114	24.41576677036966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	28.5
8	57.0
9	213.0
10	369.0
11	1365.5
12	2362.0
13	3892.5
14	7613.0
15	9803.0
16	9168.5
17	8534.0
18	9688.5
19	10843.0
20	11085.0
21	11327.0
22	11570.5
23	11814.0
24	13535.5
25	15257.0
26	17450.5
27	19644.0
28	20275.0
29	20906.0
30	24879.5
31	28853.0
32	32364.5
33	35876.0
34	44522.0
35	53168.0
36	56322.5
37	59477.0
38	64425.0
39	74836.5
40	80300.0
41	82070.5
42	83841.0
43	88496.5
44	93152.0
45	98347.5
46	103543.0
47	108654.5
48	113766.0
49	117911.0
50	122056.0
51	126500.5
52	130945.0
53	133775.5
54	136606.0
55	147351.5
56	158097.0
57	176800.5
58	195504.0
59	183083.0
60	170662.0
61	160876.0
62	151090.0
63	144011.5
64	114714.5
65	92496.0
66	74008.0
67	55520.0
68	43122.0
69	30724.0
70	26053.0
71	21382.0
72	20566.0
73	19750.0
74	16429.0
75	13108.0
76	9061.5
77	5015.0
78	3641.0
79	2267.0
80	1781.0
81	1295.0
82	804.0
83	313.0
84	294.0
85	275.0
86	174.5
87	74.0
88	42.5
89	11.5
90	12.0
91	11.5
92	11.0
93	7.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2281837.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.240204474834561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.46438286791957	5.641605140750175
2	10.200768318393925	1.6811283348796047
3	4.339138493250655	1.0726616528703283
4	2.2584823063589563	0.7444142402877502
5	1.253690252056224	0.5165332012525085
6	0.8136596446683554	0.4022833106993089
7	0.5583007806165987	0.32203588135183675
8	0.4026270743161774	0.265418353557577
9	0.30681684421489097	0.2275410179388747
>10	4.926026293702305	11.179493391049212
>50	3.430118047057771	20.342960680544312
>100	2.8738992990578995	42.76909474499176
>500	0.12169205757345254	6.6190247589337226
>1k	0.04793929540772374	6.2393911876955945
>5k	0.0018438190541432203	1.065820238354644
>10k+	6.146063513810735E-4	0.9105938648427893
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	17980	0.7879616291610664	No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9603	0.420845134862832	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6368	0.2790733956895256	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT	5074	0.22236470002020303	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3636	0.15934529942322787	No Hit
TGGATGGAGATTTCTAAAGAGACTTCTGGGCTGTTAAACTATAAAAGATGTT	3596	0.15759232583221325	No Hit
TGGATGGGGGGGGCTGGTGCGATGGCTCAGCGTGTAAGAACACCTGACTGCT	3010	0.13191126272384926	No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2938	0.12875591026002295	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	2904	0.12726588270766054	No Hit
TGGATGGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGAT	2890	0.12665234195080544	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT	2810	0.12314639476877622	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT	2747	0.1203854613629282	No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC	2400	0.10517841546087649	No Hit
TGGATGGGGGGGCTGGCAAGATGGCTCAGCGGTTAAGAGCATTGTCTGCTCT	2377	0.10417045564604308	No Hit
TGGATGGGGATTCGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATG	2324	0.10184776563794873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.76486795507304E-5	0.0	0.0	0.0	0.0
8	4.8206773752901717E-4	0.0	0.0	0.0	0.0
9	0.001314730193260956	0.0	0.0	0.0	0.0
10	0.009816652109681804	0.0	0.0	0.0	0.0
11	0.01551381628047928	0.0	0.0	0.0	0.0
12	0.01906358780228386	0.0	0.0	0.0	0.0
13	0.023577494799146476	0.0	0.0	0.0	0.0
14	0.03532241785894435	0.0	0.0	0.0	0.0
15	0.046892043559640766	0.0	0.0	0.0	0.0
16	0.06236203550034468	0.0	0.0	0.0	0.0
17	0.06994364628148286	0.0	0.0	0.0	0.0
18	0.07778820310127323	0.0	0.0	0.0	0.0
19	0.089401653141745	0.0	0.0	0.0	0.0
20	0.09615060146715125	0.0	0.0	0.0	0.0
21	0.10469634772334746	0.0	0.0	0.0	0.0
22	0.11420622945460171	0.0	0.0	0.0	0.0
23	0.12007869098450065	0.0	0.0	0.0	0.0
24	0.127441180066762	0.0	0.0	0.0	0.0
25	0.13278774951935654	0.0	0.0	0.0	0.0
26	0.13800284595262502	0.0	0.0	0.0	0.0
27	0.14475179427803125	0.0	0.0	0.0	0.0
28	0.15220193203984334	0.0	0.0	0.0	0.0
29	0.15798674489019154	0.0	0.0	0.0	0.0
30	0.16456039585649632	0.0	0.0	0.0	0.0
31	0.1701260870079677	0.0	0.0	0.0	0.0
32	0.17744475175045368	0.0	0.0	0.0	0.0
33	0.18388692969743237	0.0	0.0	0.0	0.0
34	0.188882904431824	0.0	0.0	0.0	0.0
35	0.20286286882016552	0.0	0.0	0.0	0.0
36	0.21460779187996337	0.0	0.0	0.0	0.0
37	0.22420532229076837	0.0	0.0	0.0	0.0
38	0.23226900080943555	0.0	0.0	0.0	0.0
39	0.2388864761155157	0.0	0.0	0.0	0.0
40	0.24624896519777706	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	20	6.3127687E-4	46.0	17
CGTATAG	245	0.0	46.0	32
TACGCCG	20	6.3127687E-4	46.0	42
AACGCGT	20	6.3127687E-4	46.0	18
GCGATAA	20	6.3127687E-4	46.0	33
TAAGGCG	25	3.418223E-5	46.0	37
CAGTACG	40	5.6152203E-9	46.0	19
CGGTAAC	20	6.3127687E-4	46.0	20
TCGACTA	25	3.418223E-5	46.0	10
TGTCGCG	40	5.6152203E-9	46.0	42
CGGACTA	20	6.3127687E-4	46.0	31
CCGCGTA	35	1.020162E-7	45.999996	38
CGCGTAT	35	1.020162E-7	45.999996	39
CGCGTAG	35	1.020162E-7	45.999996	45
TACCACG	370	0.0	44.756756	24
ACGTAAC	165	0.0	44.60606	23
CGTAACT	160	0.0	44.5625	24
TGGATGG	233030	0.0	44.48989	1
ATGGGGA	94440	0.0	44.37315	4
GATGGGG	215315	0.0	44.338947	3
>>END_MODULE