FastQCFastQC Report
Fri 17 Jun 2016
SRR1527247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218619
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC168850.7610590191465952No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA93240.4202614329003763No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT62360.28107575027528386No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT48070.21666631359417726No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT36630.1651027057822907No Hit
TGGATGGAGATTTCTAAAGAGACTTCTGGGCTGTTAAACTATAAAAGATGTT31410.14157455606392985No Hit
TGGATGGGGGGGGCTGGTGCGATGGCTCAGCGTGTAAGAACACCTGACTGCT30430.13715739385626824No Hit
TGGATGGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGAT29680.13377691257489457No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC28910.13030628512601758No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT28560.12872872719470985No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT27730.12498766124332299No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT26660.12016484128189653No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC23490.10587667373262377No Hit
TGGATGGGGGGGCTGGCAAGATGGCTCAGCGGTTAAGAGCATTGTCTGCTCT22820.10285677712126327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT551.8189894E-1246.00000420
AGTATCG206.312729E-446.00000436
GCGTAAT206.312729E-446.00000411
TACGTAA800.046.00000442
CGCTAGT501.6370905E-1146.043
CGTAACT2250.046.024
TACCACG3650.045.36986524
TGGATGG2269300.044.451331
ATGGGGA927600.044.3164064
GATGGGG2101050.044.2966653
GGATGGG2253050.044.235992
CACGACG3600.044.08333227
ATGGGGG868250.044.082124
TGGGGGG355150.043.5779275
CGTAGCG750.042.9333341
TGGGGAG285800.042.6763465
ATGGGGT112350.042.6631054
TGGGGAC173850.042.5602575
ATGGGGC210550.042.242224
CACGTTA601.8189894E-1242.16666814
Produced by FastQC (version 0.11.5)