##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2218619
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35808311386498	33.0	31.0	34.0	31.0	34.0
2	32.72909183595741	34.0	31.0	34.0	31.0	34.0
3	33.07986048979117	34.0	33.0	34.0	31.0	34.0
4	35.32593653980246	35.0	35.0	37.0	33.0	37.0
5	35.97803904140368	37.0	35.0	37.0	35.0	37.0
6	36.22567507084362	37.0	37.0	37.0	35.0	37.0
7	36.62550532561021	37.0	37.0	37.0	35.0	37.0
8	36.68274363466643	37.0	37.0	37.0	35.0	37.0
9	38.56794609619768	39.0	39.0	39.0	38.0	39.0
10	37.97616129673459	39.0	38.0	39.0	35.0	39.0
11	37.10373209640772	39.0	35.0	39.0	34.0	39.0
12	37.012529415821284	39.0	35.0	39.0	34.0	39.0
13	36.88256748905513	39.0	35.0	39.0	33.0	39.0
14	37.65158641479226	40.0	36.0	40.0	33.0	40.0
15	37.77229618965672	40.0	36.0	40.0	33.0	40.0
16	37.87039099547962	40.0	37.0	40.0	34.0	40.0
17	37.81631185886356	40.0	36.0	40.0	34.0	40.0
18	37.790919035670385	40.0	36.0	40.0	34.0	40.0
19	37.77339822655445	40.0	36.0	40.0	34.0	40.0
20	37.72049639888597	40.0	36.0	40.0	33.0	40.0
21	33.5060467795507	39.0	34.0	40.0	10.0	40.0
22	34.25435146818809	39.0	34.0	40.0	17.0	40.0
23	36.36174124534226	39.0	34.0	40.0	30.0	40.0
24	37.13374175556957	39.0	35.0	40.0	32.0	40.0
25	37.424619098637486	39.0	35.0	40.0	33.0	40.0
26	37.511468620795185	40.0	35.0	40.0	34.0	40.0
27	37.4881275243744	40.0	35.0	40.0	34.0	40.0
28	37.43596940258782	40.0	35.0	40.0	33.0	40.0
29	37.43879097763068	40.0	35.0	40.0	33.0	40.0
30	37.38202999253139	40.0	35.0	40.0	33.0	40.0
31	37.246078754396315	40.0	35.0	40.0	33.0	40.0
32	37.20838233153146	40.0	35.0	40.0	33.0	40.0
33	36.893079433647685	39.0	35.0	40.0	32.0	40.0
34	36.754363412555286	39.0	35.0	40.0	31.0	40.0
35	36.72726141802626	39.0	35.0	40.0	31.0	40.0
36	36.72589435139607	39.0	35.0	40.0	32.0	40.0
37	36.72670025813355	39.0	35.0	40.0	32.0	40.0
38	36.68311503687654	39.0	35.0	40.0	32.0	40.0
39	36.53992956879933	39.0	35.0	40.0	31.0	40.0
40	31.691771773341884	37.0	31.0	40.0	9.0	40.0
41	32.60991634886386	37.0	31.0	40.0	15.0	40.0
42	34.49221069503146	37.0	32.0	40.0	24.0	40.0
43	35.389861891564074	37.0	34.0	40.0	29.0	40.0
44	36.082118200556295	38.0	35.0	40.0	31.0	40.0
45	36.31350673549627	39.0	35.0	40.0	31.0	40.0
46	36.347668076402485	39.0	35.0	40.0	32.0	40.0
47	36.32323891573993	39.0	35.0	40.0	31.0	40.0
48	36.220193282397744	38.0	35.0	40.0	31.0	40.0
49	36.12384280491603	38.0	35.0	40.0	31.0	40.0
50	36.052497522107224	38.0	35.0	40.0	31.0	40.0
51	35.87132265612077	38.0	35.0	40.0	31.0	40.0
52	35.49564751766752	37.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	8.0
15	21.0
16	76.0
17	185.0
18	339.0
19	654.0
20	1045.0
21	1800.0
22	2896.0
23	4302.0
24	6146.0
25	8673.0
26	11889.0
27	17102.0
28	22669.0
29	31625.0
30	42855.0
31	60657.0
32	86285.0
33	94670.0
34	195631.0
35	216143.0
36	260378.0
37	234483.0
38	572373.0
39	345711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.85230406843176	1.0232040742461863	0.0859994437981465	0.03849241352390834
8	98.8001545105311	1.0384838496379956	0.08780236714821248	0.07355927268269136
9	93.29033060656201	3.3955356913467343	1.8836041699814163	1.4305295321098395
10	40.9130184137069	43.30103546395303	5.947934278035119	9.83801184430495
11	32.12052182010521	19.98220514653485	29.091204934240622	18.806068099119315
12	31.69259796296705	16.287158813658404	28.9224963817582	23.097746841616342
13	25.10859232702866	16.035200275486687	31.97299761698606	26.88320978049859
14	24.366148491471495	19.351767924100532	33.15823942731942	23.123844157108543
15	26.13359932462491	20.990985833980506	31.242047417785567	21.63336742360901
16	31.239838836681738	22.170773801179923	27.217066111847053	19.372321250291286
17	29.770907037215494	22.21499049634029	25.507894775984518	22.506207690459696
18	28.006385954505937	21.927784806674783	27.053586037079825	23.01224320173946
19	26.70206105690071	23.929931186923035	26.632603434839417	22.735404321336834
20	27.27759926332552	24.152952805326198	27.356747598393415	21.21270033295487
21	31.972772251567307	22.333081975769613	26.450598322650258	19.243547450012823
22	25.189859097032887	22.589637968484	29.97125689449157	22.249246039991544
23	25.984452490490707	22.431566663766965	30.84414223442601	20.73983861131632
24	27.051242236724736	23.452336791490563	27.000535017504134	22.495885954280567
25	25.70950667960565	25.105752722752307	25.098090298514524	24.08665029912752
26	25.278472779688627	24.32576300843002	25.746962412203267	24.64880179967809
27	23.072370695464162	21.72784060715247	28.86128713402346	26.338501563359912
28	23.802509579157125	24.786860655209388	26.343594821823846	25.06703494380964
29	26.954244960491184	24.755264423499483	25.31673982779378	22.973750788215554
30	26.654103295788957	22.44211376536485	27.721839576781772	23.18194336206442
31	26.77264550605579	23.2879552550483	26.275534465358856	23.663864773537053
32	29.161383725641944	23.086749009180938	26.791846639733997	20.960020625443125
33	30.1701644130876	22.52824842841425	26.589738932191604	20.711848226306547
34	24.8459514680078	24.35334773568603	29.975719129782984	20.824981666523186
35	23.054386535047254	27.984615655053886	29.24278571489742	19.718212095001437
36	24.282267482609676	29.875161079933054	25.554229906081215	20.28834153137605
37	25.777792401489396	28.44327935531067	24.792765229180855	20.986163014019084
38	25.858157709818585	27.52383351986078	25.713743549478302	20.904265220842337
39	25.78229970986456	26.713870204843644	24.367816195570306	23.13601388972149
40	25.2312361879169	29.514260898333607	23.56380252760839	21.69070038614111
41	22.238698938393657	27.88130814709511	25.3816901414799	24.498302773031334
42	22.228241982963276	25.780496786514494	26.705171099679575	25.286090130842652
43	22.2357691879498	24.936277927846106	26.841021374107044	25.986931510097044
44	23.49087427809822	24.269692092242966	25.909225513709206	26.330208115949606
45	24.752605111558136	25.35320395254886	25.663216622592707	24.2309743133003
46	23.788807361696623	27.559801840694593	24.558024609002267	24.093366188606517
47	23.120418602743417	25.560720430141455	27.213865922900688	24.10499504421444
48	24.350147546739663	26.176689192691487	26.751461156692518	22.72170210387633
49	24.856994373526955	25.431135314355462	26.158389520688317	23.553480791429262
50	22.6935764996153	25.661774283912653	26.33985375587246	25.30479546059959
51	21.529879623315225	26.07108295746138	27.9629805748531	24.436056844370306
52	21.69065531305736	25.718746661774734	28.292104232407638	24.29849379276027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	25.5
8	50.0
9	191.5
10	333.0
11	1224.0
12	2115.0
13	3646.5
14	7047.0
15	8916.0
16	8492.5
17	8069.0
18	9229.0
19	10389.0
20	10640.0
21	10891.0
22	11302.0
23	11713.0
24	13209.5
25	14706.0
26	16767.5
27	18829.0
28	19537.0
29	20245.0
30	24046.5
31	27848.0
32	31172.5
33	34497.0
34	42389.0
35	50281.0
36	53821.5
37	57362.0
38	61997.0
39	72401.0
40	78170.0
41	79580.0
42	80990.0
43	85594.5
44	90199.0
45	95430.5
46	100662.0
47	105521.5
48	110381.0
49	114458.0
50	118535.0
51	122558.0
52	126581.0
53	129461.0
54	132341.0
55	141552.0
56	150763.0
57	170945.5
58	191128.0
59	179317.5
60	167507.0
61	157808.0
62	148109.0
63	141782.0
64	113094.0
65	90733.0
66	73157.0
67	55581.0
68	43108.0
69	30635.0
70	25925.0
71	21215.0
72	20283.0
73	19351.0
74	16238.0
75	13125.0
76	9068.0
77	5011.0
78	3562.5
79	2114.0
80	1688.0
81	1262.0
82	803.0
83	344.0
84	319.0
85	294.0
86	171.0
87	48.0
88	27.0
89	10.0
90	14.0
91	10.5
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2218619.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.448810274363371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.9104528592565	5.8221134212831895
2	10.064782181384158	1.700708702066157
3	4.114923353754833	1.0429862012826483
4	2.1949512314100086	0.7417890606265366
5	1.2390158962109652	0.5234105117003371
6	0.7836502199351715	0.39725472178172344
7	0.5353214901907781	0.3165980794487949
8	0.38993017650327727	0.26355568652201694
9	0.3013238122024157	0.22912449484014974
>10	5.113608963226405	12.037596273656476
>50	3.4633248557384326	21.108402264534064
>100	2.728300420098631	41.634073304727245
>500	0.11717780858025814	6.6301757863477455
>1k	0.0413568736165617	5.834512338485564
>5k	0.0012532385944412636	0.8237755837870477
>10k+	6.266192972206318E-4	0.8939235689103021
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	16885	0.7610590191465952	No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9324	0.4202614329003763	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6236	0.28107575027528386	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT	4807	0.21666631359417726	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3663	0.1651027057822907	No Hit
TGGATGGAGATTTCTAAAGAGACTTCTGGGCTGTTAAACTATAAAAGATGTT	3141	0.14157455606392985	No Hit
TGGATGGGGGGGGCTGGTGCGATGGCTCAGCGTGTAAGAACACCTGACTGCT	3043	0.13715739385626824	No Hit
TGGATGGGGGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGAT	2968	0.13377691257489457	No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2891	0.13030628512601758	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	2856	0.12872872719470985	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT	2773	0.12498766124332299	No Hit
TGGATGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT	2666	0.12016484128189653	No Hit
TGGATGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC	2349	0.10587667373262377	No Hit
TGGATGGGGGGGCTGGCAAGATGGCTCAGCGGTTAAGAGCATTGTCTGCTCT	2282	0.10285677712126327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.2536541875824555E-4	0.0	0.0	0.0	0.0
8	9.916078425362804E-4	0.0	0.0	0.0	0.0
9	0.002073361852575859	0.0	0.0	0.0	0.0
10	0.011313344021663927	0.0	0.0	0.0	0.0
11	0.016992552574371714	0.0	0.0	0.0	0.0
12	0.02077869160951024	0.0	0.0	0.0	0.0
13	0.025646584654688345	0.0	0.0	0.0	0.0
14	0.03889807127767318	0.0	0.0	0.0	0.0
15	0.05034663455059206	0.0	0.0	0.0	0.0
16	0.06391363275983844	0.0	0.0	0.0	0.0
17	0.0704041568200759	0.0	0.0	0.0	0.0
18	0.0789229696491376	0.0	0.0	0.0	0.0
19	0.0885686095719905	0.0	0.0	0.0	0.0
20	0.09447318354345653	0.0	0.0	0.0	0.0
21	0.1011439999387006	0.0	0.0	0.0	0.0
22	0.1116911015365865	0.0	0.0	0.0	0.0
23	0.11723509083803933	0.0	0.0	0.0	0.0
24	0.12458200348955814	0.0	0.0	0.0	0.0
25	0.13035135820976923	0.0	0.0	0.0	0.0
26	0.13603056676247702	0.0	0.0	0.0	0.0
27	0.14175484839893646	0.0	0.0	0.0	0.0
28	0.15000322272548824	0.0	0.0	0.0	0.0
29	0.1550964811894246	0.0	0.0	0.0	0.0
30	0.1624433938409434	0.0	0.0	0.0	0.0
31	0.16753665230487974	0.0	0.0	0.0	0.0
32	0.1746581995376403	0.0	0.0	0.0	0.0
33	0.18096843126287118	0.0	0.0	0.0	0.0
34	0.18610676281055918	0.0	0.0	0.0	0.0
35	0.19782576458598794	0.0	0.0	0.0	0.0
36	0.2072460390900826	0.0	0.0	0.0	0.0
37	0.215674705751641	0.0	0.0	0.0	0.0
38	0.22297654531940816	0.0	0.0	0.0	0.0
39	0.228520534620861	0.0	0.0	0.0	0.0
40	0.23690412819866774	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	55	1.8189894E-12	46.000004	20
AGTATCG	20	6.312729E-4	46.000004	36
GCGTAAT	20	6.312729E-4	46.000004	11
TACGTAA	80	0.0	46.000004	42
CGCTAGT	50	1.6370905E-11	46.0	43
CGTAACT	225	0.0	46.0	24
TACCACG	365	0.0	45.369865	24
TGGATGG	226930	0.0	44.45133	1
ATGGGGA	92760	0.0	44.316406	4
GATGGGG	210105	0.0	44.296665	3
GGATGGG	225305	0.0	44.23599	2
CACGACG	360	0.0	44.083332	27
ATGGGGG	86825	0.0	44.08212	4
TGGGGGG	35515	0.0	43.577927	5
CGTAGCG	75	0.0	42.93333	41
TGGGGAG	28580	0.0	42.676346	5
ATGGGGT	11235	0.0	42.663105	4
TGGGGAC	17385	0.0	42.560257	5
ATGGGGC	21055	0.0	42.24222	4
CACGTTA	60	1.8189894E-12	42.166668	14
>>END_MODULE