FastQCFastQC Report
Fri 17 Jun 2016
SRR1527243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555394
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGA142260.5567047586399593No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA137920.5397210762802135No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132210.5173761854336356No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC96640.37818042931931434No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87570.34268688116196566No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA72570.2839875181674529No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG58550.2291231802219149No Hit
GCTGTGGGGACAGTCTTGTTAGATTTACTTTCCGTGAAGAAGTAGCGCCT44270.17324138665113872No Hit
GCTGTGGCCGGGGACTCCCGATTGCATTCACTCTTAACCAAGCTCCAATA40900.16005359643170486No Hit
GCTGTGGGGATGTCATAAATAGTTAATTGACATTGAGTTGAAGCAGTCAT36260.14189592681206892No Hit
GCTGTGGGGATGAATACACGGAAACAACTGTCATTTTCTTGTGTTATTTT35170.13763043976780098No Hit
GCTGTGGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTG34600.1353998639740095No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT33070.1294125289485692No Hit
GCTGTGGGGGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATGTG32780.12827767459734193No Hit
GCTGTGGGGGGGGCTGGAGAGATGGCTCAGCACTTACGAGCACTGACTGC30900.12092068776869633No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA29170.11415069456999587No Hit
GCTGTGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA29080.11379849839202878No Hit
GCTGTGGGGGCTGCTGTGGGGGTCTCTTTTATAACTTTACCTTTTAACTA27930.1092982138957828No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27750.10859382153984865No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAA25770.10084550562457295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC207.8582915E-444.00000431
ATCCGAC207.8582915E-444.00000416
CGTAACC207.8582915E-444.00000412
TTACGCG207.8582915E-444.00000432
CGCGTTA207.8582915E-444.00000432
CCGTTAT302.5289373E-644.00000439
GCGTAAG207.8582915E-444.00000431
CGAACGT254.444374E-544.038
GTCGATA254.444374E-544.021
CGTCTAA454.802132E-1044.020
GTTACGC254.444374E-544.032
CTACGCG351.4470243E-744.025
CGTCGAT351.4470243E-744.032
TATGTCG454.802132E-1044.011
CGCACGT2650.041.50943425
AGTAGCG4950.040.8888941
AATCACG1300.040.61538339
GCGCATT603.6379788E-1240.33333616
GCGTCTA603.6379788E-1240.33333617
TGGGGAT376050.040.1212585
Produced by FastQC (version 0.11.5)