##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2555394
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12517013032041	33.0	31.0	34.0	31.0	34.0
2	32.52300075839577	34.0	31.0	34.0	31.0	34.0
3	32.73553628129361	34.0	31.0	34.0	31.0	34.0
4	36.34643268317919	37.0	37.0	37.0	35.0	37.0
5	34.949169482279444	35.0	35.0	37.0	33.0	37.0
6	35.817876225740534	37.0	35.0	37.0	35.0	37.0
7	36.533813963717535	37.0	37.0	37.0	35.0	37.0
8	36.62950409995484	37.0	37.0	37.0	35.0	37.0
9	38.5786555810963	39.0	39.0	39.0	37.0	39.0
10	37.86705924800638	39.0	38.0	39.0	35.0	39.0
11	37.18791153145073	39.0	37.0	39.0	34.0	39.0
12	37.177335471555466	39.0	37.0	39.0	34.0	39.0
13	37.023779111949075	39.0	37.0	39.0	33.0	39.0
14	37.87850405847396	40.0	37.0	40.0	33.0	40.0
15	37.92340633186115	40.0	38.0	40.0	34.0	40.0
16	37.65892226404226	40.0	37.0	40.0	33.0	40.0
17	37.62016972725145	40.0	37.0	40.0	33.0	40.0
18	37.733916570204045	40.0	37.0	40.0	33.0	40.0
19	37.57423082311377	40.0	37.0	40.0	33.0	40.0
20	37.534442046901574	40.0	37.0	40.0	33.0	40.0
21	37.73593778493649	40.0	37.0	40.0	33.0	40.0
22	37.419733317054046	40.0	37.0	40.0	33.0	40.0
23	37.43483001055806	40.0	37.0	40.0	33.0	40.0
24	37.52747599783047	40.0	37.0	40.0	33.0	40.0
25	37.439005883241485	39.0	36.0	40.0	33.0	40.0
26	37.30358371350954	39.0	36.0	40.0	33.0	40.0
27	37.2422334872822	39.0	36.0	40.0	33.0	40.0
28	37.11553247757489	39.0	35.0	40.0	32.0	40.0
29	37.35852905657601	40.0	36.0	40.0	33.0	40.0
30	37.33915083153518	40.0	36.0	40.0	33.0	40.0
31	37.263818808371624	40.0	35.0	40.0	33.0	40.0
32	36.383074782205796	40.0	35.0	40.0	30.0	40.0
33	36.58230081153826	39.0	35.0	40.0	31.0	40.0
34	36.453743336643974	39.0	35.0	40.0	30.0	40.0
35	36.624159718618735	39.0	35.0	40.0	31.0	40.0
36	36.431916956837185	39.0	35.0	40.0	31.0	40.0
37	36.27324553473946	39.0	35.0	40.0	30.0	40.0
38	36.05033079047693	39.0	35.0	40.0	30.0	40.0
39	36.01038039535195	39.0	35.0	40.0	30.0	40.0
40	35.741950165023475	39.0	35.0	40.0	30.0	40.0
41	35.63664820376036	38.0	35.0	40.0	29.0	40.0
42	35.445769223845716	38.0	35.0	40.0	28.0	40.0
43	35.29722265920637	38.0	35.0	40.0	28.0	40.0
44	35.21889501188466	38.0	35.0	40.0	27.0	40.0
45	35.01962163173272	38.0	35.0	40.0	26.0	40.0
46	34.69740087047242	37.0	34.0	40.0	24.0	40.0
47	34.639631696716826	37.0	34.0	40.0	24.0	40.0
48	34.50340064976282	37.0	34.0	40.0	24.0	40.0
49	34.1620294169901	36.0	34.0	40.0	23.0	40.0
50	33.64163256233677	35.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	4.0
13	3.0
14	5.0
15	34.0
16	91.0
17	254.0
18	472.0
19	918.0
20	1581.0
21	2808.0
22	4434.0
23	6634.0
24	9847.0
25	13982.0
26	19605.0
27	27575.0
28	37040.0
29	45472.0
30	53697.0
31	61036.0
32	74281.0
33	101610.0
34	181182.0
35	180870.0
36	248062.0
37	399874.0
38	745739.0
39	338281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.18583983526611	0.4845828079740345	0.26958660777946575	0.05999074898039206
8	98.51791935020587	0.7491995363532982	0.34746109601885267	0.3854200174219709
9	92.7346233105345	3.4732021754766587	1.993430367293654	1.7987441466951866
10	40.75125010076724	42.68919000357675	6.871699628315634	9.687860267340378
11	24.927467153793113	25.505303683111098	28.60474744794736	20.962481715148424
12	32.401422246432446	16.882954252847114	30.639384768063167	20.076238732657274
13	27.905872832134694	16.69441189890874	29.605571587003805	25.794143681952765
14	23.857416899311808	22.83103897089842	28.248403181662006	25.063140948127767
15	27.088973363794388	20.9584901584648	30.69526656163394	21.25726991610687
16	30.41902735938176	23.366651091769018	28.486918259963044	17.727403288886176
17	30.85238518991592	21.545718585861906	29.29736079837395	18.304535425848226
18	33.124676664342175	20.675911425009215	26.449345971697515	19.750065938951096
19	29.860757284395284	21.852285792327915	28.813599781481834	19.473357141794963
20	30.416092391232034	24.147196087961387	25.471845046204226	19.96486647460235
21	28.925598166075368	22.205068963924937	30.117703962676597	18.7516289073231
22	27.377813362636054	23.36046809220026	25.846816577013172	23.41490196815051
23	23.25171773902576	28.09273247100056	27.451970224552458	21.203579565421222
24	25.107400267825625	26.63902317998712	28.351714060532345	19.901862491654907
25	26.398825386613574	24.74843409665985	25.545727977759984	23.307012538966593
26	27.234899980198747	26.773014259249255	26.203239109115856	19.78884665143614
27	25.187857528036773	24.512032195426613	25.897024098827814	24.4030861777088
28	25.57609511488248	28.68007829712365	25.11276147631246	20.63106511168141
29	29.6112849916686	26.018023052413835	24.02482748257216	20.3458644733454
30	26.260960149393792	29.023195640280914	24.653028065339434	20.062816144985863
31	27.13464146820412	27.8640788856826	24.496300766144085	20.504978879969194
32	24.90038718099831	28.769496993418624	26.91714076185512	19.41297506372794
33	27.575591083018903	27.768672854362187	24.817816743719366	19.83791931889955
34	22.461546047302296	29.986686984472843	26.601572986396622	20.950193981828242
35	21.690745145367014	33.471355102187765	26.809329598488528	18.02857015395669
36	24.58845876604547	33.08926138200215	25.187896660945437	17.134383191006943
37	24.39005491912402	31.356299654769483	25.511682347223168	18.741963078883334
38	24.41717402482748	32.04640849904163	24.44664110505073	19.089776371080156
39	23.767998203016834	31.137233632073958	23.939009013874184	21.155759151035024
40	23.582899545040803	30.899188148676878	25.448326168097758	20.06958613818456
41	23.097455813076184	32.05274803024504	24.013596337785874	20.836199818892897
42	21.452699661969934	31.20990344346116	23.57464250131291	23.762754393255992
43	22.009326154792568	30.44411155383475	25.647395274466483	21.899167016906198
44	23.02537299531892	31.023943861494548	24.410951892350063	21.539731250836468
45	22.624886808061692	31.694016656531243	25.022481856026896	20.658614679380165
46	24.412673740331236	32.58648177149981	23.66112622945816	19.33971825871079
47	23.29965555213795	30.690648878411704	24.69873530265783	21.310960266792517
48	23.896549807974818	30.63147992051324	25.162616801949135	20.309353469562815
49	24.492778804364416	32.03866018312636	24.338477745506175	19.130083267003055
50	21.973519543365917	32.627845255956615	24.934432811535128	20.46420238914234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	19.5
8	39.0
9	116.0
10	193.0
11	1727.5
12	3262.0
13	9763.5
14	16265.0
15	12779.0
16	9293.0
17	10729.0
18	12165.0
19	12307.5
20	12450.0
21	12718.0
22	12986.0
23	14469.5
24	15953.0
25	15731.5
26	15510.0
27	18781.0
28	22052.0
29	28021.5
30	33991.0
31	43019.0
32	52047.0
33	55112.0
34	58177.0
35	61971.5
36	65766.0
37	81166.0
38	96566.0
39	100940.5
40	105315.0
41	110851.0
42	116387.0
43	107656.5
44	98926.0
45	101741.0
46	104556.0
47	107646.5
48	110737.0
49	114962.0
50	119187.0
51	123309.5
52	127432.0
53	133101.0
54	138770.0
55	143119.5
56	147469.0
57	153690.0
58	159911.0
59	174367.0
60	188823.0
61	183748.0
62	178673.0
63	170717.0
64	162761.0
65	150803.0
66	138845.0
67	117231.5
68	95618.0
69	75904.5
70	56191.0
71	43894.0
72	31597.0
73	25610.5
74	19624.0
75	16844.0
76	14064.0
77	11016.5
78	7969.0
79	5718.5
80	3468.0
81	2286.5
82	1105.0
83	965.5
84	826.0
85	551.0
86	276.0
87	177.0
88	78.0
89	61.0
90	44.0
91	28.0
92	12.0
93	8.0
94	4.0
95	7.5
96	11.0
97	5.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2555394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.739707627350349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.83848096568053	7.500448667096816
2	10.122233836953258	2.1741966388910137
3	4.401099822129466	1.417995759853623
4	2.437990522472697	1.0473322163843157
5	1.4844965878760248	0.7971529663793856
6	1.0248721927770341	0.6604096623496074
7	0.7020287085162816	0.5277708152829858
8	0.5126581068271704	0.44046385440918007
9	0.4255202774722391	0.41129670326047585
>10	4.578858705742445	12.199634751893287
>50	2.283248927599428	17.60114859025413
>100	2.0601573902473085	39.65233718376124
>500	0.09129939111238584	6.611266495071441
>1k	0.034380523849852414	5.972979191365374
>5k	0.0015280232822156626	1.2936825779511112
>10k+	0.001146017461661747	1.6918839257960192
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGA	14226	0.5567047586399593	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA	13792	0.5397210762802135	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13221	0.5173761854336356	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9664	0.37818042931931434	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8757	0.34268688116196566	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA	7257	0.2839875181674529	No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5855	0.2291231802219149	No Hit
GCTGTGGGGACAGTCTTGTTAGATTTACTTTCCGTGAAGAAGTAGCGCCT	4427	0.17324138665113872	No Hit
GCTGTGGCCGGGGACTCCCGATTGCATTCACTCTTAACCAAGCTCCAATA	4090	0.16005359643170486	No Hit
GCTGTGGGGATGTCATAAATAGTTAATTGACATTGAGTTGAAGCAGTCAT	3626	0.14189592681206892	No Hit
GCTGTGGGGATGAATACACGGAAACAACTGTCATTTTCTTGTGTTATTTT	3517	0.13763043976780098	No Hit
GCTGTGGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTG	3460	0.1353998639740095	No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3307	0.1294125289485692	No Hit
GCTGTGGGGGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATGTG	3278	0.12827767459734193	No Hit
GCTGTGGGGGGGGCTGGAGAGATGGCTCAGCACTTACGAGCACTGACTGC	3090	0.12092068776869633	No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA	2917	0.11415069456999587	No Hit
GCTGTGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	2908	0.11379849839202878	No Hit
GCTGTGGGGGCTGCTGTGGGGGTCTCTTTTATAACTTTACCTTTTAACTA	2793	0.1092982138957828	No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2775	0.10859382153984865	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAA	2577	0.10084550562457295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.652594472711449E-4	0.0	0.0	0.0	0.0
8	0.0017609808898353834	0.0	0.0	0.0	0.0
9	0.00794398045859073	0.0	0.0	0.0	0.0
10	0.04124608573081098	0.0	0.0	0.0	0.0
11	0.06856085597759093	0.0	0.0	0.0	0.0
12	0.08562280415466264	0.0	0.0	0.0	0.0
13	0.09755834129688025	0.0	0.0	0.0	0.0
14	0.1371999777725079	0.0	0.0	0.0	0.0
15	0.1566099004693601	0.0	0.0	0.0	0.0
16	0.18020704439315424	0.0	0.0	0.0	0.0
17	0.19425575860317432	0.0	0.0	0.0	0.0
18	0.20783487790923827	0.0	0.0	0.0	0.0
19	0.22697087024544943	0.0	0.0	0.0	0.0
20	0.24501114113909636	0.0	0.0	0.0	0.0
21	0.2623470196768091	0.0	0.0	0.0	0.0
22	0.28308746126820367	0.0	0.0	0.0	0.0
23	0.30781945954322504	0.0	0.0	0.0	0.0
24	0.3314166034670192	0.0	0.0	0.0	0.0
25	0.3503569312599153	0.0	0.0	0.0	0.0
26	0.37172349938991794	0.0	0.0	0.0	0.0
27	0.39195521316869336	0.0	0.0	0.0	0.0
28	0.47119935321128564	0.0	0.0	0.0	0.0
29	0.4908440733601159	0.0	0.0	0.0	0.0
30	0.5285290644025931	0.0	0.0	0.0	0.0
31	0.5547089802981459	0.0	0.0	0.0	0.0
32	0.5800671051117753	0.0	0.0	0.0	0.0
33	0.6042512426655146	0.0	0.0	0.0	0.0
34	0.6346575126966723	0.0	0.0	0.0	0.0
35	0.7650874972704796	0.0	0.0	0.0	0.0
36	0.7977634760040917	0.0	0.0	0.0	0.0
37	0.8271131575013482	0.0	0.0	0.0	0.0
38	0.8555236491906923	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	20	7.8582915E-4	44.000004	31
ATCCGAC	20	7.8582915E-4	44.000004	16
CGTAACC	20	7.8582915E-4	44.000004	12
TTACGCG	20	7.8582915E-4	44.000004	32
CGCGTTA	20	7.8582915E-4	44.000004	32
CCGTTAT	30	2.5289373E-6	44.000004	39
GCGTAAG	20	7.8582915E-4	44.000004	31
CGAACGT	25	4.444374E-5	44.0	38
GTCGATA	25	4.444374E-5	44.0	21
CGTCTAA	45	4.802132E-10	44.0	20
GTTACGC	25	4.444374E-5	44.0	32
CTACGCG	35	1.4470243E-7	44.0	25
CGTCGAT	35	1.4470243E-7	44.0	32
TATGTCG	45	4.802132E-10	44.0	11
CGCACGT	265	0.0	41.509434	25
AGTAGCG	495	0.0	40.88889	41
AATCACG	130	0.0	40.615383	39
GCGCATT	60	3.6379788E-12	40.333336	16
GCGTCTA	60	3.6379788E-12	40.333336	17
TGGGGAT	37605	0.0	40.121258	5
>>END_MODULE