FastQCFastQC Report
Fri 17 Jun 2016
SRR1527242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2715323
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGAAG143100.5270091256178363No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA133820.49283271271962853No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129540.4770703153915759No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC92140.3393334789268164No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66660.2454956555813065No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA64790.23860881375806858No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT46090.16974039552568884No Hit
GCTGTGGGGACAGTCTTGTTAGATTTACTTTCCGTGAAGAAGTAGCGCCTCA41760.1537938580419346No Hit
GCTGTGGCCGGGGACTCCCGATTGCATTCACTCTTAACCAAGCTCCAATAAA38030.14005700242659896No Hit
GCTGTGGGGATGAATACACGGAAACAACTGTCATTTTCTTGTGTTATTTTTT36900.13589543490774394No Hit
GCTGTGGGGATGTCATAAATAGTTAATTGACATTGAGTTGAAGCAGTCATAC36030.13269139619853698No Hit
GCTGTGGGGGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATGTGTG32820.12086959820249747No Hit
GCTGTGGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTGAA31810.11714996705732615No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC30240.11136796616829747No Hit
GCTGTGGGGGCTGCTGTGGGGGTCTCTTTTATAACTTTACCTTTTAACTAAA27980.1030448311305874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA551.8189894E-1246.00000420
AACGTCG453.110472E-1046.035
CGTATAG1000.046.031
ACCGCTA206.3129805E-446.033
ATTCGGC253.4183955E-546.039
GTTACGC301.8623468E-646.032
ACGATAG253.4183955E-546.038
TACGCCG206.3129805E-446.015
CTACCGA253.4183955E-546.038
TACTCGA206.3129805E-446.011
CGTCGAT301.8623468E-646.032
ACGACGT253.4183955E-546.015
TACGTCG301.8623468E-646.044
TACGTAA405.6170393E-946.037
TACCGAC206.3129805E-446.013
CGACCGT351.020253E-745.99999615
TCGATCA601.8189894E-1242.16666820
CCGTATA1200.042.16666830
TGGGGAT403400.042.1001475
CACGGAA4400.041.81818417
Produced by FastQC (version 0.11.5)