##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2715323
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14431911047047	33.0	31.0	34.0	31.0	34.0
2	32.51300453021611	34.0	31.0	34.0	31.0	34.0
3	32.68619571225965	34.0	31.0	34.0	31.0	34.0
4	36.30596139022871	37.0	37.0	37.0	35.0	37.0
5	35.160931498757236	35.0	35.0	37.0	33.0	37.0
6	35.97763433668849	37.0	35.0	37.0	35.0	37.0
7	36.55978386365084	37.0	37.0	37.0	35.0	37.0
8	36.602005728231966	37.0	37.0	37.0	35.0	37.0
9	38.54551410642491	39.0	39.0	39.0	37.0	39.0
10	37.7661699915627	39.0	37.0	39.0	35.0	39.0
11	37.15830750153849	39.0	37.0	39.0	34.0	39.0
12	37.17116527205051	39.0	37.0	39.0	34.0	39.0
13	37.07119926432325	39.0	37.0	39.0	33.0	39.0
14	37.95987328211045	40.0	38.0	40.0	34.0	40.0
15	37.908090492365	40.0	38.0	40.0	34.0	40.0
16	32.78056533237482	39.0	34.0	40.0	3.0	40.0
17	34.38203042510965	39.0	34.0	40.0	17.0	40.0
18	36.46784305218937	39.0	34.0	40.0	30.0	40.0
19	37.20618651998307	39.0	36.0	40.0	32.0	40.0
20	37.68347301591744	39.0	37.0	40.0	33.0	40.0
21	37.89914349047977	40.0	37.0	40.0	34.0	40.0
22	37.83983489257079	40.0	37.0	40.0	34.0	40.0
23	37.74188006362411	40.0	37.0	40.0	33.0	40.0
24	37.7266454119823	40.0	37.0	40.0	34.0	40.0
25	37.62377256775713	40.0	36.0	40.0	33.0	40.0
26	37.45446232363516	40.0	36.0	40.0	33.0	40.0
27	37.41507916369434	40.0	36.0	40.0	33.0	40.0
28	37.33618689194619	40.0	36.0	40.0	33.0	40.0
29	37.130575257529216	40.0	35.0	40.0	33.0	40.0
30	37.050030880304114	39.0	35.0	40.0	32.0	40.0
31	36.94583406835945	39.0	35.0	40.0	32.0	40.0
32	36.71418759388846	39.0	35.0	40.0	32.0	40.0
33	36.391311088957	39.0	35.0	40.0	31.0	40.0
34	36.19405499824514	39.0	35.0	40.0	30.0	40.0
35	36.289270926515925	39.0	35.0	40.0	30.0	40.0
36	36.23768074737333	39.0	35.0	40.0	31.0	40.0
37	35.80678504914517	38.0	35.0	40.0	30.0	40.0
38	35.7759688258082	38.0	35.0	40.0	30.0	40.0
39	35.37538480689038	38.0	35.0	40.0	28.0	40.0
40	35.207942480507846	38.0	34.0	40.0	28.0	40.0
41	35.18193857599998	38.0	34.0	40.0	28.0	40.0
42	35.020492221367405	37.0	34.0	40.0	27.0	40.0
43	34.878553306549534	37.0	34.0	40.0	27.0	40.0
44	35.13593410434044	37.0	35.0	40.0	28.0	40.0
45	35.05043709348759	37.0	35.0	40.0	27.0	40.0
46	34.95443856955508	37.0	35.0	40.0	27.0	40.0
47	34.65786501274434	37.0	34.0	40.0	25.0	40.0
48	34.584251302699535	37.0	34.0	40.0	25.0	40.0
49	34.447105924414885	36.0	34.0	40.0	24.0	40.0
50	33.85102729951464	36.0	34.0	40.0	22.0	40.0
51	33.88752498321563	36.0	34.0	40.0	23.0	40.0
52	33.51818512935662	35.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	13.0
15	43.0
16	95.0
17	213.0
18	479.0
19	898.0
20	1695.0
21	3128.0
22	5323.0
23	8160.0
24	12275.0
25	17782.0
26	25534.0
27	35186.0
28	42618.0
29	51296.0
30	58436.0
31	69110.0
32	90115.0
33	129905.0
34	201419.0
35	228928.0
36	313948.0
37	494223.0
38	637946.0
39	286551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.18190211624915	0.48642463530121466	0.27322716302996	0.058446085419671986
8	98.50584994860648	0.749118981425046	0.34975581173952414	0.3952752582289473
9	92.78093250784529	3.4276953423220737	1.9890451338570032	1.8023270159756317
10	40.95033997796948	42.65116894012241	6.731758984106126	9.666732097801994
11	24.903151485108772	25.549851711932615	28.44777582630133	21.09922097665729
12	32.71028897851195	16.796381130348028	30.563030622876177	19.93029926826385
13	27.94908745663039	16.6313547228083	29.424639352298048	25.99491846826326
14	23.824090172697687	22.887000920332497	28.08115277629954	25.207756130670273
15	27.31837059532144	20.968444638078047	30.55872174323276	21.154463023367754
16	28.83929462535396	30.575404841339314	25.13100651377387	15.454294019532853
17	30.97115886397309	21.399074806201693	29.292021612161793	18.337744717663423
18	33.42791999331203	20.518074645263194	26.335430444186564	19.718574917238207
19	29.877808275479566	21.737819036630267	28.891590429573206	19.492782258316964
20	30.32129142647118	24.173993296561772	25.412630467903817	20.09208480906323
21	29.007856523883163	22.13685075403552	30.099881303255632	18.75541141882568
22	27.07729430347697	23.27229578212242	25.880088667167772	23.770321247232832
23	23.265887704704006	28.118570055938097	27.32790905538678	21.287633183971117
24	25.25728983255399	26.707135762485716	28.12044828552625	19.915126119434042
25	26.483368645277196	24.570299739662648	25.354957771138093	23.591373843922067
26	27.404585016220906	26.60085006461478	26.043089532994784	19.95147538616953
27	25.269553566923715	24.31511831189144	25.743677639824064	24.671650481360782
28	25.61584754373605	28.688262869647552	24.992533116686303	20.703356469930096
29	30.038231179126758	25.879794042918654	23.79602721296877	20.285947564985822
30	26.30714651627081	28.982187386178364	24.651873828638436	20.05879226891239
31	27.475405320103725	27.59244480306763	24.43153171832596	20.50061815850269
32	24.91165876030218	28.55240426277095	27.056375981789277	19.479560995137597
33	28.191821009876172	27.521882295402794	24.56565940773897	19.720637286982065
34	22.647766030045045	29.826580484163394	26.38794721659265	21.137706269198915
35	21.762051881120588	33.56263693122328	26.657565232570857	18.01774595508527
36	24.731680172119486	32.97755000049718	25.103348662387493	17.18742116499584
37	24.620901454449434	31.28242938317099	25.341699679927583	18.754969482451997
38	24.518630011972792	31.903644612445735	24.4790767065281	19.098648669053368
39	24.194543337938065	30.910392612591576	23.774188190502564	21.120875858967793
40	23.728484603857442	30.7799477262926	25.36545376001308	20.126113909836878
41	23.33541902749691	31.86821604648876	23.90665861851426	20.889706307500067
42	21.521343869587522	31.12664681144748	23.449806892218717	23.902202426746282
43	22.04603282924352	30.279086502784384	25.747581411124937	21.92729925684716
44	23.24132340793342	30.891057896242913	24.356439362830866	21.511179332992796
45	22.733280718352844	31.525678528852737	25.00266082524989	20.738379927544532
46	24.315744388420825	32.70918413757774	23.52593043258574	19.4491410414157
47	23.814625368694628	30.548778174824875	24.49947943577983	21.137117020700668
48	24.11672570813859	30.489521872720115	25.05381496050378	20.339937458637518
49	24.54337844889908	31.939404630682976	24.33982992078659	19.177386999631352
50	21.96659476607387	32.37029259502461	24.936038916917067	20.727073721984457
51	20.6227767377951	33.13730999958384	25.716535380873655	20.5233778817474
52	22.762227550829127	32.09375090919202	24.478782082278975	20.665239457699876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	18.5
8	37.0
9	117.0
10	197.0
11	1640.5
12	3084.0
13	9717.5
14	12869.5
15	9388.0
16	11025.5
17	12663.0
18	12732.0
19	12801.0
20	13034.5
21	13268.0
22	13888.5
23	14509.0
24	14632.5
25	14756.0
26	16965.0
27	19174.0
28	24637.5
29	30101.0
30	37587.5
31	45074.0
32	46949.5
33	48825.0
34	57569.5
35	66314.0
36	76705.0
37	87096.0
38	96603.5
39	113743.5
40	121376.0
41	108164.5
42	94953.0
43	97440.5
44	99928.0
45	103224.0
46	106520.0
47	109520.0
48	112520.0
49	116650.0
50	120780.0
51	128521.5
52	136263.0
53	137443.5
54	138624.0
55	149640.0
56	160656.0
57	164593.0
58	168530.0
59	178133.5
60	187737.0
61	187617.5
62	187498.0
63	176370.0
64	156005.5
65	146769.0
66	127557.5
67	108346.0
68	86306.5
69	64267.0
70	51930.0
71	39593.0
72	30816.0
73	22039.0
74	19072.0
75	16105.0
76	13522.0
77	10939.0
78	7609.0
79	4279.0
80	2791.5
81	1304.0
82	982.0
83	660.0
84	546.5
85	433.0
86	304.5
87	176.0
88	97.5
89	13.0
90	7.0
91	5.5
92	4.0
93	4.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2715323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.90105876196167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79239303317668	9.390989399448218
2	9.58182662533285	2.4723141668069593
3	4.081916937150706	1.5798315080288359
4	2.214670885333339	1.1428639692036435
5	1.3101999097598822	0.8451483012864559
6	0.8819834325273737	0.6827112054067382
7	0.6465337571237323	0.5838678994571588
8	0.44322592366153357	0.45744669487857453
9	0.38553717803751053	0.44764540099045214
>10	3.9806043550432353	12.816467749918353
>50	1.9421744762993458	17.926394954860182
>100	1.6467592635359325	37.89120416165848
>500	0.06611790555708144	5.878956732793865
>1k	0.024102093651349882	5.236734357826769
>5k	9.771119047844547E-4	0.9395086418404417
>10k+	9.771119047844547E-4	1.7079148555949102
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGAAG	14310	0.5270091256178363	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA	13382	0.49283271271962853	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12954	0.4770703153915759	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9214	0.3393334789268164	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6666	0.2454956555813065	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA	6479	0.23860881375806858	No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4609	0.16974039552568884	No Hit
GCTGTGGGGACAGTCTTGTTAGATTTACTTTCCGTGAAGAAGTAGCGCCTCA	4176	0.1537938580419346	No Hit
GCTGTGGCCGGGGACTCCCGATTGCATTCACTCTTAACCAAGCTCCAATAAA	3803	0.14005700242659896	No Hit
GCTGTGGGGATGAATACACGGAAACAACTGTCATTTTCTTGTGTTATTTTTT	3690	0.13589543490774394	No Hit
GCTGTGGGGATGTCATAAATAGTTAATTGACATTGAGTTGAAGCAGTCATAC	3603	0.13269139619853698	No Hit
GCTGTGGGGGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATGTGTG	3282	0.12086959820249747	No Hit
GCTGTGGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTGAA	3181	0.11714996705732615	No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3024	0.11136796616829747	No Hit
GCTGTGGGGGCTGCTGTGGGGGTCTCTTTTATAACTTTACCTTTTAACTAAA	2798	0.1030448311305874	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.733886539465102E-4	0.0	0.0	0.0	0.0
8	0.0018414015570155005	0.0	0.0	0.0	0.0
9	0.007255122134641072	0.0	0.0	0.0	0.0
10	0.03476566139645265	0.0	0.0	0.0	0.0
11	0.059366786198179734	0.0	0.0	0.0	0.0
12	0.07461359109026808	0.0	0.0	0.0	0.0
13	0.08326817840824094	0.0	0.0	0.0	0.0
14	0.11943330498802536	0.0	0.0	0.0	0.0
15	0.13703710387309356	0.0	0.0	0.0	0.0
16	0.16384791054323924	0.0	0.0	0.0	0.0
17	0.1758538486949803	0.0	0.0	0.0	0.0
18	0.1898116724971578	0.0	0.0	0.0	0.0
19	0.20789423578705002	0.0	0.0	0.0	0.0
20	0.22546120664097788	0.0	0.0	0.0	0.0
21	0.24498006314534218	0.0	0.0	0.0	0.0
22	0.26468305980540807	0.0	0.0	0.0	0.0
23	0.28777423533038243	0.0	0.0	0.0	0.0
24	0.3108654108553568	0.0	0.0	0.0	0.0
25	0.3291321143009506	0.0	0.0	0.0	0.0
26	0.34677274121715906	0.0	0.0	0.0	0.0
27	0.36758057881143424	0.0	0.0	0.0	0.0
28	0.44458799192582243	0.0	0.0	0.0	0.0
29	0.46193399459290846	0.0	0.0	0.0	0.0
30	0.49647868780251925	0.0	0.0	0.0	0.0
31	0.5204537360748611	0.0	0.0	0.0	0.0
32	0.5458282495305347	0.0	0.0	0.0	0.0
33	0.5703557182699811	0.0	0.0	0.0	0.0
34	0.5981240537497748	0.0	0.0	0.0	0.0
35	0.7236339838759515	0.0	0.0	0.0	0.0
36	0.7565582437153886	0.0	0.0	0.0	0.0
37	0.7833690503855343	0.0	0.0	0.0	0.0
38	0.8113215260210296	0.0	0.0	0.0	0.0
39	0.8371379758503869	0.0	0.0	0.0	0.0
40	0.8654219037661449	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAA	55	1.8189894E-12	46.000004	20
AACGTCG	45	3.110472E-10	46.0	35
CGTATAG	100	0.0	46.0	31
ACCGCTA	20	6.3129805E-4	46.0	33
ATTCGGC	25	3.4183955E-5	46.0	39
GTTACGC	30	1.8623468E-6	46.0	32
ACGATAG	25	3.4183955E-5	46.0	38
TACGCCG	20	6.3129805E-4	46.0	15
CTACCGA	25	3.4183955E-5	46.0	38
TACTCGA	20	6.3129805E-4	46.0	11
CGTCGAT	30	1.8623468E-6	46.0	32
ACGACGT	25	3.4183955E-5	46.0	15
TACGTCG	30	1.8623468E-6	46.0	44
TACGTAA	40	5.6170393E-9	46.0	37
TACCGAC	20	6.3129805E-4	46.0	13
CGACCGT	35	1.020253E-7	45.999996	15
TCGATCA	60	1.8189894E-12	42.166668	20
CCGTATA	120	0.0	42.166668	30
TGGGGAT	40340	0.0	42.100147	5
CACGGAA	440	0.0	41.818184	17
>>END_MODULE