FastQCFastQC Report
Fri 17 Jun 2016
SRR1527241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647085
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGAAG141660.535154707914555No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA123110.4650776231212825No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117150.4425622902173523No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC86920.3283611973170487No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68420.25847299954478226No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA66560.2514464023633544No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT45370.17139608286095837No Hit
GCTGTGGGGACAGTCTTGTTAGATTTACTTTCCGTGAAGAAGTAGCGCCTCA40950.1546984702040169No Hit
GCTGTGGCCGGGGACTCCCGATTGCATTCACTCTTAACCAAGCTCCAATAAA38520.14551856098311916No Hit
GCTGTGGGGATGTCATAAATAGTTAATTGACATTGAGTTGAAGCAGTCATAC34880.13176758585387321No Hit
GCTGTGGGGGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATGTGTG33650.12712096513712254No Hit
GCTGTGGGGATGAATACACGGAAACAACTGTCATTTTCTTGTGTTATTTTTT33410.12621430743629314No Hit
GCTGTGGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTGAA32840.12406099539682329No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC30110.11374776404988883No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA27260.10298120385253969No Hit
GCTGTGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG26870.10150788508869191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA351.020253E-746.00000429
TACGACG301.8623305E-646.027
ACGTACG206.312952E-446.033
CGACTCG206.312952E-446.019
TCGCGAC206.312952E-446.020
TACGTCG253.4183722E-546.044
CGACCGT650.042.4615415
TGGGGAT374600.042.242395
CGATACC1050.041.61904520
ATTATCG508.731149E-1041.39999844
CGTCATA508.731149E-1041.39999843
CGACGTA451.5897967E-840.8888930
AGTAGCG6050.040.2975241
AACCGAT402.912493E-740.2536
CCGATAA402.912493E-740.2544
CGATACG402.912493E-740.2524
TGGGGAC163900.040.1061675
GATATTC6550.039.679399
GTCGTAC1450.039.6551725
AACGTCG355.3757667E-639.42857435