##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527241_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2647085
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14519329753295	33.0	31.0	34.0	30.0	34.0
2	32.5260461979876	34.0	31.0	34.0	31.0	34.0
3	32.65248339210868	34.0	31.0	34.0	31.0	34.0
4	36.29641511322833	37.0	37.0	37.0	35.0	37.0
5	35.23300951801699	35.0	35.0	37.0	33.0	37.0
6	35.97824852620902	37.0	35.0	37.0	35.0	37.0
7	36.564976568565044	37.0	37.0	37.0	35.0	37.0
8	36.62866398321172	37.0	37.0	37.0	35.0	37.0
9	38.48815243938143	39.0	39.0	39.0	37.0	39.0
10	37.7576791073955	39.0	37.0	39.0	35.0	39.0
11	37.06582448240234	39.0	37.0	39.0	34.0	39.0
12	37.117901389641816	39.0	37.0	39.0	34.0	39.0
13	36.96314474223533	39.0	37.0	39.0	33.0	39.0
14	37.87986936573627	40.0	37.0	40.0	33.0	40.0
15	38.00921693107701	40.0	38.0	40.0	34.0	40.0
16	37.93916704601477	40.0	37.0	40.0	34.0	40.0
17	37.863532905063494	40.0	37.0	40.0	34.0	40.0
18	37.95759259713987	40.0	38.0	40.0	34.0	40.0
19	37.780810967535984	40.0	37.0	40.0	34.0	40.0
20	37.93840772019032	40.0	38.0	40.0	34.0	40.0
21	33.69880415627001	39.0	34.0	40.0	10.0	40.0
22	34.32346222354023	39.0	34.0	40.0	16.0	40.0
23	36.28684005235948	39.0	34.0	40.0	30.0	40.0
24	37.10114257759007	39.0	36.0	40.0	32.0	40.0
25	37.32510176288257	39.0	36.0	40.0	33.0	40.0
26	37.3421779051296	39.0	36.0	40.0	33.0	40.0
27	37.38181093542519	40.0	36.0	40.0	33.0	40.0
28	37.319582484128766	40.0	36.0	40.0	33.0	40.0
29	37.305601066833894	40.0	36.0	40.0	33.0	40.0
30	37.13026140074837	39.0	35.0	40.0	33.0	40.0
31	36.929686428656424	39.0	35.0	40.0	32.0	40.0
32	36.72293560652567	39.0	35.0	40.0	32.0	40.0
33	36.42132081138309	39.0	35.0	40.0	31.0	40.0
34	36.210887825664834	39.0	35.0	40.0	30.0	40.0
35	36.29887442224182	39.0	35.0	40.0	30.0	40.0
36	36.188397425847675	39.0	35.0	40.0	30.0	40.0
37	35.9587096749821	38.0	35.0	40.0	30.0	40.0
38	35.86432849719597	38.0	35.0	40.0	30.0	40.0
39	35.69000239886517	38.0	35.0	40.0	30.0	40.0
40	30.806650334235584	36.0	29.0	40.0	8.0	40.0
41	31.699540437877893	36.0	29.0	40.0	13.0	40.0
42	33.35914109293808	35.0	30.0	40.0	22.0	40.0
43	34.15895522810941	36.0	33.0	40.0	25.0	40.0
44	34.84896367135925	37.0	34.0	40.0	27.0	40.0
45	34.93769221615475	37.0	34.0	40.0	27.0	40.0
46	34.915276993371954	37.0	34.0	40.0	26.0	40.0
47	34.8532083404953	37.0	34.0	40.0	26.0	40.0
48	34.688651856664976	37.0	34.0	40.0	26.0	40.0
49	34.4973006155828	36.0	34.0	40.0	25.0	40.0
50	34.26858298845711	36.0	34.0	40.0	24.0	40.0
51	34.05555205065194	36.0	34.0	40.0	23.0	40.0
52	33.63077951784699	35.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	13.0
15	39.0
16	93.0
17	240.0
18	483.0
19	1000.0
20	1894.0
21	3427.0
22	5559.0
23	8531.0
24	12360.0
25	18131.0
26	25902.0
27	35996.0
28	44134.0
29	53541.0
30	61642.0
31	77553.0
32	100978.0
33	123778.0
34	223274.0
35	257614.0
36	328393.0
37	350873.0
38	615387.0
39	296249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.19394352655846	0.4812463521194068	0.26776624097828366	0.05704388034384994
8	98.51814354280273	0.7547169811320755	0.3310056156111345	0.39613386045404664
9	92.73986290580015	3.434759367379589	2.022148892083178	1.8032288347370786
10	41.07892266398699	42.535430482965225	6.753390994244612	9.632255858803173
11	25.043056796438346	25.5906402703351	28.33856865193222	21.02773428129433
12	32.802271177540575	16.765158655653295	30.5551578434391	19.877412323367025
13	28.180205773520683	16.58004937506729	29.329809960768166	25.90993489064386
14	23.829306576857185	22.898773556572607	28.02807616680235	25.243843699767858
15	27.25473492539907	20.90318217964289	30.615601690161064	21.226481204796972
16	30.400912702085503	23.24844120985915	28.605806009251687	17.744840078803666
17	31.038104178747567	21.402977237225098	29.29955026000298	18.259368324024354
18	33.457935804857044	20.51241271058542	26.335799568204273	19.69385191635327
19	29.98181017987711	21.77553799745758	28.753628991891077	19.489022830774232
20	30.408997066584565	24.174932047894192	25.30440087870242	20.111670006818823
21	33.65619162210507	21.138837627050133	28.072577948951395	17.132392801893403
22	27.270563657759382	23.30831839551809	25.780320616829457	23.640797329893072
23	23.27450761875799	28.166908127241854	27.31695431011849	21.241629943881666
24	25.38770005496612	26.712138068856873	28.084628940891587	19.815532935285418
25	26.476293734428623	24.621083191510664	25.378520145745227	23.52410292831549
26	27.436822013648975	26.634392171010752	26.000638438131002	19.92814737720927
27	25.334811689084408	24.3848233056362	25.695774786227116	24.584590219052277
28	25.745036521305508	28.697945098098476	24.90105153404594	20.655966846550072
29	29.95653709646649	25.925763623004173	23.841017572159565	20.27668170836977
30	26.43009196909053	29.0133108683703	24.56494596886764	19.99165119367153
31	27.560203015770178	27.611580285483843	24.433442824843176	20.394773873902803
32	24.907889244206363	28.66723206848288	26.961846710626975	19.463031976683787
33	28.17064809025777	27.60508257196123	24.50695765341876	19.717311684362233
34	22.610947513963474	30.0031166358466	26.268971340172303	21.116964510017624
35	21.740253901933638	33.67145369340236	26.60957241645055	17.97871998821345
36	24.794481476794285	33.010009123243115	25.11351165527363	17.08199774468897
37	24.563888201550007	31.432840275246164	25.31018082154521	18.693090701658615
38	24.473449095892274	32.04770530602531	24.412665252532502	19.066180345549917
39	23.882383829759906	31.073010500229497	23.802257955449107	21.24234771456149
40	24.80449248890761	33.95467089269895	23.11569141149604	18.125145206897397
41	23.346738015590734	32.014310080711425	23.813175625263263	20.825776278434578
42	21.48389643702412	31.247882104276968	23.39101313331457	23.877208325384338
43	22.11345687803754	30.366724151283393	25.647419708849544	21.87239926182952
44	23.202730550775666	31.0144177463134	24.31368845352529	21.469163249385645
45	22.676075758806384	31.616400682259922	24.972224163561048	20.735299395372646
46	24.320903937727728	32.84061524280482	23.443183728516463	19.39529709095099
47	23.560558123369667	30.799124319770616	24.41224970108629	21.22806785577343
48	24.08596626100031	30.62005942385681	25.007054930234577	20.286919384908305
49	24.614963252030066	32.014725632157635	24.266240033848554	19.104071081963745
50	21.86000827325152	32.65040601265165	25.033461335771236	20.45612437832559
51	20.642442535846033	33.27705759354158	25.62101330331289	20.4594865672995
52	22.698213317668305	32.29499619392653	24.38353887389336	20.623251614511812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	14.5
8	29.0
9	101.5
10	174.0
11	1525.5
12	2877.0
13	8868.5
14	12110.5
15	9361.0
16	10895.5
17	12430.0
18	12353.0
19	12276.0
20	12546.5
21	12817.0
22	13508.0
23	14199.0
24	14293.0
25	14387.0
26	16197.5
27	18008.0
28	22930.0
29	27852.0
30	36010.0
31	44168.0
32	45883.0
33	47598.0
34	55431.5
35	63265.0
36	73189.5
37	83114.0
38	92509.0
39	110538.0
40	119172.0
41	106662.5
42	94153.0
43	95619.0
44	97085.0
45	100439.0
46	103793.0
47	106538.0
48	109283.0
49	113735.5
50	118188.0
51	125632.5
52	133077.0
53	133903.0
54	134729.0
55	145865.5
56	157002.0
57	160067.5
58	163133.0
59	172683.5
60	182234.0
61	182933.5
62	183633.0
63	172860.0
64	153241.5
65	144396.0
66	125825.5
67	107255.0
68	85568.5
69	63882.0
70	51607.5
71	39333.0
72	30498.5
73	21664.0
74	18861.5
75	16059.0
76	13398.0
77	10737.0
78	7476.5
79	4216.0
80	2779.5
81	1343.0
82	993.5
83	644.0
84	534.5
85	425.0
86	310.5
87	196.0
88	107.0
89	13.5
90	9.0
91	8.0
92	7.0
93	6.0
94	5.0
95	6.5
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2647085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.07165699964811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.49603937079155	9.476433604879722
2	9.742711734972406	2.5470677209201162
3	4.0607294569143795	1.5924138788745632
4	2.2216341678301257	1.1616175928229624
5	1.3429637412521953	0.8777380694306437
6	0.8949562539620707	0.7019136708889292
7	0.6187002965109042	0.5661206643099784
8	0.4493763272783633	0.4699274571155501
9	0.36631641025679634	0.43095262213972974
>10	4.151364202579862	13.592398800424437
>50	1.9867521613182837	18.575999683030776
>100	1.575075898500386	36.31442971900201
>500	0.06894722377616735	6.077473827227259
>1k	0.022424582490306856	4.973787115116354
>5k	0.0010040857831480681	0.9708173045443759
>10k+	0.0010040857831480681	1.6709082692725912
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGAAG	14166	0.535154707914555	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA	12311	0.4650776231212825	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11715	0.4425622902173523	No Hit
GCTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	8692	0.3283611973170487	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6842	0.25847299954478226	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA	6656	0.2514464023633544	No Hit
GCTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4537	0.17139608286095837	No Hit
GCTGTGGGGACAGTCTTGTTAGATTTACTTTCCGTGAAGAAGTAGCGCCTCA	4095	0.1546984702040169	No Hit
GCTGTGGCCGGGGACTCCCGATTGCATTCACTCTTAACCAAGCTCCAATAAA	3852	0.14551856098311916	No Hit
GCTGTGGGGATGTCATAAATAGTTAATTGACATTGAGTTGAAGCAGTCATAC	3488	0.13176758585387321	No Hit
GCTGTGGGGGTGCAACACTGCAGTCATTGCTCCAGTTTAAATTACATGTGTG	3365	0.12712096513712254	No Hit
GCTGTGGGGATGAATACACGGAAACAACTGTCATTTTCTTGTGTTATTTTTT	3341	0.12621430743629314	No Hit
GCTGTGGGGATATTCCAAATTAACTAGGCCTATCTGGTTTCTTTCACTTGAA	3284	0.12406099539682329	No Hit
GCTGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3011	0.11374776404988883	No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA	2726	0.10298120385253969	No Hit
GCTGTGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2687	0.10150788508869191	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.177706798232773E-4	0.0	0.0	0.0	0.0
8	0.0016244283806526802	0.0	0.0	0.0	0.0
9	0.007177706798232773	0.0	0.0	0.0	0.0
10	0.040837373941524355	0.0	0.0	0.0	0.0
11	0.06671489581936356	0.0	0.0	0.0	0.0
12	0.08424361136873203	0.0	0.0	0.0	0.0
13	0.0942924008862579	0.0	0.0	0.0	0.0
14	0.13089870555724503	0.0	0.0	0.0	0.0
15	0.1484274211066135	0.0	0.0	0.0	0.0
16	0.1715471924777633	0.0	0.0	0.0	0.0
17	0.1836359618221553	0.0	0.0	0.0	0.0
18	0.19614028261276084	0.0	0.0	0.0	0.0
19	0.21162901833526312	0.0	0.0	0.0	0.0
20	0.22945995311824138	0.0	0.0	0.0	0.0
21	0.2464597850087927	0.0	0.0	0.0	0.0
22	0.2678417957866861	0.0	0.0	0.0	0.0
23	0.29186822485866526	0.0	0.0	0.0	0.0
24	0.3157813217180408	0.0	0.0	0.0	0.0
25	0.3335744790968178	0.0	0.0	0.0	0.0
26	0.35182096532600954	0.0	0.0	0.0	0.0
27	0.3722963184030736	0.0	0.0	0.0	0.0
28	0.4513644253962377	0.0	0.0	0.0	0.0
29	0.46987535345483805	0.0	0.0	0.0	0.0
30	0.5042150138737517	0.0	0.0	0.0	0.0
31	0.5273725626491027	0.0	0.0	0.0	0.0
32	0.5500390051698377	0.0	0.0	0.0	0.0
33	0.573725437604006	0.0	0.0	0.0	0.0
34	0.5998296239070525	0.0	0.0	0.0	0.0
35	0.7096107605158126	0.0	0.0	0.0	0.0
36	0.7414571122574455	0.0	0.0	0.0	0.0
37	0.7649168802664063	0.0	0.0	0.0	0.0
38	0.7906810699316418	0.0	0.0	0.0	0.0
39	0.8152741600666393	0.0	0.0	0.0	0.0
40	0.8453827512150157	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTAA	35	1.020253E-7	46.000004	29
TACGACG	30	1.8623305E-6	46.0	27
ACGTACG	20	6.312952E-4	46.0	33
CGACTCG	20	6.312952E-4	46.0	19
TCGCGAC	20	6.312952E-4	46.0	20
TACGTCG	25	3.4183722E-5	46.0	44
CGACCGT	65	0.0	42.46154	15
TGGGGAT	37460	0.0	42.24239	5
CGATACC	105	0.0	41.619045	20
ATTATCG	50	8.731149E-10	41.399998	44
CGTCATA	50	8.731149E-10	41.399998	43
CGACGTA	45	1.5897967E-8	40.88889	30
AGTAGCG	605	0.0	40.29752	41
AACCGAT	40	2.912493E-7	40.25	36
CCGATAA	40	2.912493E-7	40.25	44
CGATACG	40	2.912493E-7	40.25	24
TGGGGAC	16390	0.0	40.106167	5
GATATTC	655	0.0	39.67939	9
GTCGTAC	145	0.0	39.65517	25
AACGTCG	35	5.3757667E-6	39.428574	35
>>END_MODULE