FastQCFastQC Report
Fri 17 Jun 2016
SRR1527240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817887
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC59420.7265062288555754No Hit
TCGGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT45330.5542330419727909No Hit
TCGGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC42750.5226883420325791No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG35980.43991407125923265No Hit
TCGGGTGGGATATCATAATTTATTTTAAAGAGAAAGTGATTCAGTGGGGA33950.4150940166551125No Hit
TCGGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG29150.356406202812858No Hit
TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA29060.3553058063033157No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT25560.3125126087100052No Hit
TCGGGTGGGACTTAAAGAGGTGTGATTTTTGCTAAGTCACTGTTTTAAAG21770.26617368903039174No Hit
TCGGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC21340.26091623904035643No Hit
TCGGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC21010.2568814518387014No Hit
TCGGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT20800.2543138599831028No Hit
TCGGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC19650.2402532379167293No Hit
TCGGGTGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACC19200.23475125536901795No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGG18680.22839340886944037No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG18010.2202015681872924No Hit
TCGGGTGGGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAAT15000.18339941825704528No Hit
TCGGGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC14630.17887556594003817No Hit
TCGGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC14590.17838650082468605No Hit
TCGGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC13810.1688497310753197No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT13560.16579307410436894No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG13370.16347001480644638No Hit
TCGGGTGGCTATTAATTATTCGGTCTGCATTCCCAGTGGGCAGTGATGGC12990.15882389621060122No Hit
TCGGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG12810.15662310319151668No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC12760.15601177179732653No Hit
TCGGGTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC12660.1547891090089462No Hit
TCGGGTGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTG12260.14989845785542502No Hit
TCGGGTGGGAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCG11930.14586367065377No Hit
TCGGGTGGGAAGTCTGTGATGTATTATTAATAATGCCTTATATATTGTTT11700.14305154624049535No Hit
TCGGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG11680.14280701368281926No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT11270.13779409625046005No Hit
TCGGGTGGGAATGGGAAGGCGGGGAGGTGCTTGGAGATTTCCTAAACCAC11040.13498197183718533No Hit
TCGGGTGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGC10370.12679013115503732No Hit
TCGGGTGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC10260.12544520208781898No Hit
TCGGGTGGGAATTAGAAAGTATCTTGTTATATACACAGACACTTTTATGG10220.12495613697246685No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT10170.12434480557827672No Hit
TCGGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT9810.11994321954010763No Hit
TCGGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC9650.11798695907869915No Hit
TCGGGTGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG9610.117497893963347No Hit
TCGGGTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT9610.117497893963347No Hit
TCGGGTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT9370.1145635032712343No Hit
TCGGGTGGGGCCGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCAAC9310.11382990559820612No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGC9280.11346310676169202No Hit
TCGGGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT9240.11297404164633988No Hit
TCGGGTGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTC9130.11162911257912157No Hit
TCGGGTGGGTACTACCTCACTCTTTACGTACGTGGTGACTGTACGCACAG8940.109306053281199No Hit
TCGGGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG8840.10808339049281869No Hit
TCGGGTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT8670.10600486375257218No Hit
TCGGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC8450.10331500561813552No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT8410.10282594050278339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT408.307325E-944.00000443
ACGCGAA207.8551465E-444.00000443
AACGTTA207.8551465E-444.00000419
AACAATT207.8551465E-444.00000427
AGCGTAT302.5268164E-644.00000424
AACCGGT207.8551465E-444.00000413
AACCGAT207.8551465E-444.00000412
CTCACGA207.8551465E-444.00000426
TCGTTAC302.5268164E-644.00000439
GGTACGC207.8551465E-444.0000049
CGTATTA600.044.00000410
AATTTCG207.8551465E-444.00000439
CGTATAC302.5268164E-644.00000434
GATCGAT207.8551465E-444.0000049
CGAAATT207.8551465E-444.00000433
CGACGTA207.8551465E-444.00000416
CAACGAA207.8551465E-444.00000436
ACGCATA207.8551465E-444.00000410
ACGTTAA207.8551465E-444.00000420
TAGACGA207.8551465E-444.00000429
Produced by FastQC (version 0.11.5)