##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527240_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	817887
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39815646904768	31.0	31.0	33.0	31.0	33.0
2	32.200438446875914	31.0	31.0	34.0	31.0	34.0
3	32.743322732846956	34.0	31.0	34.0	31.0	34.0
4	36.470542996770945	37.0	37.0	37.0	35.0	37.0
5	36.413491105739546	37.0	37.0	37.0	35.0	37.0
6	31.539557420523863	33.0	32.0	35.0	28.0	35.0
7	34.706985194776294	35.0	35.0	35.0	32.0	35.0
8	36.03400347480765	37.0	35.0	37.0	35.0	37.0
9	38.70012361120791	39.0	39.0	39.0	39.0	39.0
10	38.14267007545052	39.0	38.0	39.0	37.0	39.0
11	37.646857084169326	39.0	37.0	39.0	35.0	39.0
12	37.01254085222042	39.0	35.0	39.0	34.0	39.0
13	36.810691452486715	39.0	35.0	39.0	33.0	39.0
14	37.5726255582984	39.0	37.0	40.0	33.0	40.0
15	37.52314684057822	39.0	37.0	40.0	33.0	40.0
16	37.35500747658295	39.0	36.0	40.0	33.0	40.0
17	37.29107199405297	39.0	36.0	40.0	33.0	40.0
18	37.227900675765724	39.0	36.0	40.0	33.0	40.0
19	37.20154251137382	39.0	36.0	40.0	32.0	40.0
20	36.80075242667997	39.0	35.0	40.0	31.0	40.0
21	37.091962581628025	39.0	35.0	40.0	32.0	40.0
22	36.88883916726883	39.0	35.0	40.0	32.0	40.0
23	36.98364566254263	39.0	35.0	40.0	32.0	40.0
24	37.01437973705414	38.0	35.0	40.0	32.0	40.0
25	36.956904804697956	38.0	35.0	40.0	32.0	40.0
26	36.72655513536711	38.0	35.0	40.0	32.0	40.0
27	36.548443733669814	38.0	35.0	40.0	31.0	40.0
28	36.31644591490022	38.0	35.0	40.0	31.0	40.0
29	36.84805235931125	39.0	35.0	40.0	32.0	40.0
30	36.98917821166005	39.0	35.0	40.0	32.0	40.0
31	36.99531231086935	39.0	35.0	40.0	32.0	40.0
32	36.21451007290738	39.0	35.0	40.0	30.0	40.0
33	36.35535715814043	39.0	35.0	40.0	31.0	40.0
34	36.23084362509736	38.0	35.0	40.0	30.0	40.0
35	36.22641269515226	38.0	35.0	40.0	30.0	40.0
36	36.0337870634941	38.0	35.0	40.0	30.0	40.0
37	35.96388620921961	38.0	35.0	40.0	30.0	40.0
38	35.79382970997216	38.0	34.0	40.0	30.0	40.0
39	35.71980236878689	37.0	34.0	40.0	30.0	40.0
40	35.60732228290705	37.0	34.0	40.0	30.0	40.0
41	35.47071294689853	37.0	34.0	40.0	29.0	40.0
42	35.31255051125645	37.0	34.0	40.0	29.0	40.0
43	35.12883686866279	36.0	34.0	40.0	29.0	40.0
44	35.06138133996506	36.0	34.0	40.0	29.0	40.0
45	34.917019099215416	36.0	34.0	40.0	28.0	40.0
46	34.570436991907194	35.0	33.0	40.0	27.0	40.0
47	34.42652102307532	35.0	33.0	39.0	27.0	40.0
48	34.317816519886	35.0	33.0	39.0	27.0	40.0
49	33.990861818319644	35.0	33.0	39.0	26.0	40.0
50	33.46855127908868	35.0	32.0	38.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	3.0
15	8.0
16	36.0
17	87.0
18	151.0
19	331.0
20	588.0
21	796.0
22	1148.0
23	1737.0
24	2756.0
25	3943.0
26	4772.0
27	6496.0
28	8773.0
29	11531.0
30	15661.0
31	21459.0
32	30328.0
33	45792.0
34	82073.0
35	78083.0
36	110669.0
37	156718.0
38	216388.0
39	17558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.58832943915236	0.344057308650217	0.03985880690119784	0.027754445296232853
8	99.21272743056193	0.5322251117819454	0.1772861043151438	0.0777613533409872
9	97.83258567503825	1.1368318606360048	0.38464971322444297	0.6459327511013135
10	62.08901718697082	24.8453637238396	4.85201500940839	8.213604079781192
11	35.83429006696524	25.343721076383414	18.91777225949306	19.904216597158285
12	31.324009306909144	15.995485928985302	27.553806332659647	25.12669843144591
13	24.455945625740476	16.409601815409708	31.118112893345902	28.016339665503914
14	24.49604896519935	17.603042963147722	30.706686865055932	27.194221206596996
15	25.82165996036127	18.896375660696403	29.446977394187705	25.834986984754615
16	31.33562460339876	19.343381176128243	25.622610458412957	23.69838376206004
17	32.17889512854465	19.610166196552825	22.887880599642738	25.323058075259787
18	29.542711890517882	21.79347513776353	23.85121661060758	24.81259636111101
19	33.561604475923936	19.659928572039902	22.582092636268825	24.19637431576734
20	31.095126832924354	20.50099830416671	23.393084863801477	25.01078999910746
21	32.63421475093748	18.97242528613366	23.705108407396132	24.688251555532734
22	31.11566756776914	19.525802464154584	24.991594193329885	24.36693577474639
23	31.690319078307883	19.162060284611442	25.109458886129747	24.038161750950927
24	32.55510846852927	18.361827489616537	24.452155371096495	24.630908670757695
25	29.537576706806686	18.59890180428348	23.81698205253293	28.04653943637691
26	26.334200201250297	19.731943410275502	24.87079510983791	29.063061278636294
27	27.013756179032068	19.304011434342396	24.47624182802759	29.20599055859795
28	28.762286232694738	21.348059083956585	23.081672651601014	26.807982031747663
29	30.27215251006557	21.299030306142537	22.449922788844916	25.978894394946977
30	29.453824305802634	18.72544740288084	24.15957216583709	27.661156125479437
31	32.029241203246904	19.301810641323314	23.486007235718382	25.182940919711406
32	33.02986842925734	20.498552978589952	21.947041583984095	24.524537008168608
33	33.103594995396676	19.21439025195412	23.644586599371305	24.037428153277897
34	29.95560511415391	22.077988768619626	26.0037144495511	21.96269166767536
35	29.252696277114076	24.83619375292675	23.806711685110535	22.10439828484864
36	31.066394257397416	24.834482025023018	21.265773878298592	22.833349839280974
37	29.876254299188034	24.99954150145436	21.90021359918913	23.223990600168484
38	32.18170725295793	24.41278562931065	21.194859436572532	22.21064768115889
39	31.549957390201826	22.199888248621143	20.542935637808156	25.707218723368875
40	28.29645171032184	22.235956800878363	22.413731970308856	27.053859518490942
41	26.98416773955326	22.746296248748298	23.891686748902966	26.377849262795472
42	25.36181648565144	21.717058713489763	22.33462568790065	30.586499112958148
43	25.517216926054576	19.981855684220438	26.576409699628435	27.924517690096557
44	26.422965519686702	20.077467914271775	25.20678284408482	28.2927837219567
45	27.720699803273558	20.05362598989836	24.856856754050376	27.368817452777705
46	27.890405398300743	21.360530244398067	23.709999058549652	27.03906529875154
47	26.346671361691776	21.61900115786166	24.62626255216185	27.408064928284716
48	27.934421258682434	21.125167657634858	25.425150418089544	25.51526066559317
49	28.27334338362145	20.629377896946643	24.97093119220626	26.126347527225647
50	25.88328216489564	19.858366742594026	25.561355052715108	28.69699603979523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	14.5
12	29.0
13	95.0
14	161.0
15	234.0
16	307.0
17	738.5
18	1170.0
19	1050.0
20	930.0
21	1068.0
22	1206.0
23	1627.5
24	2049.0
25	1907.0
26	1765.0
27	1908.0
28	2051.0
29	2758.5
30	3466.0
31	4535.5
32	5605.0
33	6886.5
34	8168.0
35	10969.0
36	13770.0
37	10934.5
38	8099.0
39	11323.0
40	14547.0
41	14938.0
42	15329.0
43	16935.5
44	18542.0
45	18738.0
46	18934.0
47	19518.0
48	20102.0
49	23902.0
50	27702.0
51	27204.5
52	26707.0
53	30908.0
54	35109.0
55	37921.5
56	40734.0
57	44821.0
58	48908.0
59	60501.0
60	72094.0
61	73616.5
62	75139.0
63	78747.5
64	82356.0
65	84441.5
66	86527.0
67	71153.5
68	55780.0
69	48334.0
70	40888.0
71	33126.0
72	25364.0
73	24185.0
74	23006.0
75	18741.0
76	14476.0
77	15229.5
78	15983.0
79	11891.0
80	7799.0
81	4787.0
82	1775.0
83	1184.0
84	593.0
85	426.5
86	260.0
87	324.0
88	388.0
89	220.5
90	53.0
91	33.5
92	14.0
93	8.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	817887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.04478613793837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.8210090682247	7.113818901633111
2	11.29328707930132	2.2687730701184883
3	4.5158541780780235	1.360823683467276
4	2.1374231635323473	0.8587983425583241
5	1.2452072302355304	0.6253920162565245
6	0.7145030734587061	0.43062183406754234
7	0.4381960927515063	0.3081110226718361
8	0.34325360598867993	0.27583272505859613
9	0.24100785101332844	0.2178785088893698
>10	3.2864706956362975	8.908076543581203
>50	2.348000730326821	17.366824512432647
>100	2.4745907126772564	45.051455763448985
>500	0.09737690950033473	6.548092829449545
>1k	0.042602397906396444	7.938994017510977
>5k	0.001217211368754184	0.7265062288555754
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5942	0.7265062288555754	No Hit
TCGGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4533	0.5542330419727909	No Hit
TCGGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4275	0.5226883420325791	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3598	0.43991407125923265	No Hit
TCGGGTGGGATATCATAATTTATTTTAAAGAGAAAGTGATTCAGTGGGGA	3395	0.4150940166551125	No Hit
TCGGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2915	0.356406202812858	No Hit
TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2906	0.3553058063033157	No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2556	0.3125126087100052	No Hit
TCGGGTGGGACTTAAAGAGGTGTGATTTTTGCTAAGTCACTGTTTTAAAG	2177	0.26617368903039174	No Hit
TCGGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	2134	0.26091623904035643	No Hit
TCGGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2101	0.2568814518387014	No Hit
TCGGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	2080	0.2543138599831028	No Hit
TCGGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1965	0.2402532379167293	No Hit
TCGGGTGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACC	1920	0.23475125536901795	No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGG	1868	0.22839340886944037	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1801	0.2202015681872924	No Hit
TCGGGTGGGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAAT	1500	0.18339941825704528	No Hit
TCGGGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	1463	0.17887556594003817	No Hit
TCGGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1459	0.17838650082468605	No Hit
TCGGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1381	0.1688497310753197	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1356	0.16579307410436894	No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1337	0.16347001480644638	No Hit
TCGGGTGGCTATTAATTATTCGGTCTGCATTCCCAGTGGGCAGTGATGGC	1299	0.15882389621060122	No Hit
TCGGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1281	0.15662310319151668	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1276	0.15601177179732653	No Hit
TCGGGTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1266	0.1547891090089462	No Hit
TCGGGTGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTG	1226	0.14989845785542502	No Hit
TCGGGTGGGAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCG	1193	0.14586367065377	No Hit
TCGGGTGGGAAGTCTGTGATGTATTATTAATAATGCCTTATATATTGTTT	1170	0.14305154624049535	No Hit
TCGGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1168	0.14280701368281926	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1127	0.13779409625046005	No Hit
TCGGGTGGGAATGGGAAGGCGGGGAGGTGCTTGGAGATTTCCTAAACCAC	1104	0.13498197183718533	No Hit
TCGGGTGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGC	1037	0.12679013115503732	No Hit
TCGGGTGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1026	0.12544520208781898	No Hit
TCGGGTGGGAATTAGAAAGTATCTTGTTATATACACAGACACTTTTATGG	1022	0.12495613697246685	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT	1017	0.12434480557827672	No Hit
TCGGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	981	0.11994321954010763	No Hit
TCGGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	965	0.11798695907869915	No Hit
TCGGGTGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTG	961	0.117497893963347	No Hit
TCGGGTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	961	0.117497893963347	No Hit
TCGGGTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT	937	0.1145635032712343	No Hit
TCGGGTGGGGCCGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCAAC	931	0.11382990559820612	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGC	928	0.11346310676169202	No Hit
TCGGGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	924	0.11297404164633988	No Hit
TCGGGTGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTC	913	0.11162911257912157	No Hit
TCGGGTGGGTACTACCTCACTCTTTACGTACGTGGTGACTGTACGCACAG	894	0.109306053281199	No Hit
TCGGGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	884	0.10808339049281869	No Hit
TCGGGTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	867	0.10600486375257218	No Hit
TCGGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	845	0.10331500561813552	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT	841	0.10282594050278339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.890651153521208E-4	0.0	0.0	0.0	0.0
9	6.11331394190151E-4	0.0	0.0	0.0	0.0
10	0.005012917432359238	0.0	0.0	0.0	0.0
11	0.011126231374260748	0.0	0.0	0.0	0.0
12	0.015038752297077714	0.0	0.0	0.0	0.0
13	0.018095409268028467	0.0	0.0	0.0	0.0
14	0.022252462748521495	0.0	0.0	0.0	0.0
15	0.027143113902042704	0.0	0.0	0.0	0.0
16	0.036679883651409055	0.0	0.0	0.0	0.0
17	0.040592404574226026	0.0	0.0	0.0	0.0
18	0.043282262708662686	0.0	0.0	0.0	0.0
19	0.047439316189155714	0.0	0.0	0.0	0.0
20	0.05196316850616283	0.0	0.0	0.0	0.0
21	0.056364754544331916	0.0	0.0	0.0	0.0
22	0.06027727546714889	0.0	0.0	0.0	0.0
23	0.06406753011112783	0.0	0.0	0.0	0.0
24	0.06626832313021236	0.0	0.0	0.0	0.0
25	0.06956951265883918	0.0	0.0	0.0	0.0
26	0.07238163707211387	0.0	0.0	0.0	0.0
27	0.07580509287957872	0.0	0.0	0.0	0.0
28	0.08094027659077599	0.0	0.0	0.0	0.0
29	0.0866867916961634	0.0	0.0	0.0	0.0
30	0.08998798122479022	0.0	0.0	0.0	0.0
31	0.09622356144552976	0.0	0.0	0.0	0.0
32	0.10453766840651582	0.0	0.0	0.0	0.0
33	0.10857245560817082	0.0	0.0	0.0	0.0
34	0.11334084048285399	0.0	0.0	0.0	0.0
35	0.12214401255919216	0.0	0.0	0.0	0.0
36	0.13143624975088244	0.0	0.0	0.0	0.0
37	0.13840542764465016	0.0	0.0	0.0	0.0
38	0.14366287763468547	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	40	8.307325E-9	44.000004	43
ACGCGAA	20	7.8551465E-4	44.000004	43
AACGTTA	20	7.8551465E-4	44.000004	19
AACAATT	20	7.8551465E-4	44.000004	27
AGCGTAT	30	2.5268164E-6	44.000004	24
AACCGGT	20	7.8551465E-4	44.000004	13
AACCGAT	20	7.8551465E-4	44.000004	12
CTCACGA	20	7.8551465E-4	44.000004	26
TCGTTAC	30	2.5268164E-6	44.000004	39
GGTACGC	20	7.8551465E-4	44.000004	9
CGTATTA	60	0.0	44.000004	10
AATTTCG	20	7.8551465E-4	44.000004	39
CGTATAC	30	2.5268164E-6	44.000004	34
GATCGAT	20	7.8551465E-4	44.000004	9
CGAAATT	20	7.8551465E-4	44.000004	33
CGACGTA	20	7.8551465E-4	44.000004	16
CAACGAA	20	7.8551465E-4	44.000004	36
ACGCATA	20	7.8551465E-4	44.000004	10
ACGTTAA	20	7.8551465E-4	44.000004	20
TAGACGA	20	7.8551465E-4	44.000004	29
>>END_MODULE