FastQCFastQC Report
Fri 17 Jun 2016
SRR1527239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889564
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC56920.6398640232743231No Hit
TCGGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG45840.515308623100755No Hit
TCGGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT40300.4530309230139709No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT33660.37838761460670617No Hit
TCGGGTGGGATATCATAATTTATTTTAAAGAGAAAGTGATTCAGTGGGGAAA33300.3743406882472762No Hit
TCGGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG28030.31509818293006464No Hit
TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC27770.3121754027815874No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC25530.2869945276562451No Hit
TCGGGTGGGACTTAAAGAGGTGTGATTTTTGCTAAGTCACTGTTTTAAAGGA24370.2739544316091928No Hit
TCGGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT21140.237644508995418No Hit
TCGGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19380.21785953568264904No Hit
TCGGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG18580.20886636599502678No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT18270.20538151274107314No Hit
TCGGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA17790.1999856109284998No Hit
TCGGGTGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA17440.19605109919016506No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTG17120.19245383131511618No Hit
TCGGGTGGGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTG17030.19144209972525866No Hit
TCGGGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT14160.15917910347091385No Hit
TCGGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC14150.15906668884981856No Hit
TCGGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA13340.14996110454110104No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT12980.14591417818167102No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG12860.1445652027285277No Hit
TCGGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC12720.1429913980331938No Hit
TCGGGTGGCTATTAATTATTCGGTCTGCATTCCCAGTGGGCAGTGATGGCAT12650.14220449568552684No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC12600.14164242258005044No Hit
TCGGGTGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTG12270.13793274008390627No Hit
TCGGGTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT11940.1342230575877621No Hit
TCGGGTGGGAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGAC11630.13073820433380848No Hit
TCGGGTGGGAATTAGAAAGTATCTTGTTATATACACAGACACTTTTATGGTC10930.12286918085713901No Hit
TCGGGTGGGAATGGGAAGGCGGGGAGGTGCTTGGAGATTTCCTAAACCACGC10900.12253193699385317No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT10830.12174503464618622No Hit
TCGGGTGGGAAGTCTGTGATGTATTATTAATAATGCCTTATATATTGTTTGT10430.1172484498023751No Hit
TCGGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT10280.11556223048594594No Hit
TCGGGTGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC10130.11387601116951676No Hit
TCGGGTGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTC9990.11230220647418286No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT9640.10836769473584812No Hit
TCGGGTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG9620.10814286549365756No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC9460.10634423155613311No Hit
TCGGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA9400.10566974382956146No Hit
TCGGGTGGGGCCGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCAACTG9340.10499525610298976No Hit
TCGGGTGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG9010.10128557360684559No Hit
TCGGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT8990.10106074436465505No Hit
TCGGGTGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCC8970.1008359151224645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT206.310681E-446.033
AACGTAG253.4165292E-546.016
TAATACG253.4165292E-546.029
ACTATCG253.4165292E-546.021
ACTATCC453.092282E-1046.032
TGATACA301.8609244E-646.042
GGTACGC301.8609244E-646.09
GTACCGA206.310681E-446.028
ACACGTT253.4165292E-546.044
CAAACCG253.4165292E-546.013
CATATGA1850.046.031
AATTTCG301.8609244E-646.039
CATATCG206.310681E-446.017
CGTATAC206.310681E-446.034
GGTAAGT206.310681E-446.013
ACGTTTC206.310681E-446.019
ACGTTTA301.8609244E-646.022
ACCATTA253.4165292E-546.025
ACGCATA206.310681E-446.010
AACCCTA750.046.045
Produced by FastQC (version 0.11.5)