##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	889564
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.600801066589923	31.0	31.0	33.0	31.0	33.0
2	32.242515434527476	33.0	31.0	34.0	31.0	34.0
3	32.76361565890706	34.0	31.0	34.0	31.0	34.0
4	36.43264340733213	37.0	37.0	37.0	35.0	37.0
5	36.40140001169112	37.0	37.0	37.0	35.0	37.0
6	31.48323335926364	33.0	32.0	35.0	28.0	35.0
7	34.713532696916694	35.0	35.0	35.0	32.0	35.0
8	36.03597492704291	37.0	35.0	37.0	35.0	37.0
9	38.68572019551151	39.0	39.0	39.0	39.0	39.0
10	38.13330238184099	39.0	38.0	39.0	37.0	39.0
11	37.78828954409126	39.0	38.0	39.0	35.0	39.0
12	37.225260914335564	39.0	37.0	39.0	35.0	39.0
13	37.11407386090264	39.0	37.0	39.0	34.0	39.0
14	37.959227216928745	40.0	38.0	40.0	34.0	40.0
15	37.824098097494954	40.0	37.0	40.0	33.0	40.0
16	32.795951724665116	39.0	34.0	40.0	3.0	40.0
17	34.31030257519414	39.0	34.0	40.0	16.0	40.0
18	36.29871600019785	39.0	34.0	40.0	30.0	40.0
19	37.259481049143176	39.0	36.0	40.0	33.0	40.0
20	37.44774181509144	39.0	36.0	40.0	33.0	40.0
21	37.671490752773266	40.0	37.0	40.0	33.0	40.0
22	37.68663974711207	40.0	37.0	40.0	33.0	40.0
23	37.69302152515165	40.0	36.0	40.0	34.0	40.0
24	37.67557027937281	40.0	36.0	40.0	34.0	40.0
25	37.615609444626806	40.0	36.0	40.0	34.0	40.0
26	37.46542688328215	40.0	36.0	40.0	33.0	40.0
27	37.333355441542146	39.0	36.0	40.0	33.0	40.0
28	37.186879190255	39.0	35.0	40.0	33.0	40.0
29	37.00458876483311	39.0	35.0	40.0	33.0	40.0
30	37.004831580414674	39.0	35.0	40.0	32.0	40.0
31	36.972557342698224	39.0	35.0	40.0	32.0	40.0
32	36.80739553309262	39.0	35.0	40.0	32.0	40.0
33	36.43256584124357	38.0	35.0	40.0	31.0	40.0
34	36.223880462788514	38.0	35.0	40.0	31.0	40.0
35	36.14431901470833	38.0	35.0	40.0	30.0	40.0
36	36.08146687590775	38.0	35.0	40.0	31.0	40.0
37	35.743693539756556	38.0	34.0	40.0	30.0	40.0
38	35.753312858883675	37.0	34.0	40.0	30.0	40.0
39	35.35293244780589	37.0	34.0	40.0	29.0	40.0
40	35.31708567343103	37.0	34.0	40.0	29.0	40.0
41	35.26680598585374	36.0	34.0	40.0	29.0	40.0
42	35.138947844112394	36.0	34.0	40.0	29.0	40.0
43	34.94840281306348	36.0	33.0	39.0	29.0	40.0
44	35.38437256903382	36.0	34.0	40.0	30.0	40.0
45	35.46318870817614	36.0	34.0	40.0	30.0	40.0
46	35.391732354276925	36.0	34.0	40.0	30.0	40.0
47	35.05503707434204	35.0	34.0	39.0	30.0	40.0
48	34.995837286580844	35.0	34.0	39.0	30.0	40.0
49	34.91451205309567	35.0	34.0	39.0	30.0	40.0
50	34.45021943334038	35.0	34.0	39.0	28.0	40.0
51	34.487971635542806	35.0	34.0	39.0	29.0	40.0
52	34.098561767337706	35.0	33.0	38.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	9.0
16	28.0
17	75.0
18	145.0
19	244.0
20	440.0
21	697.0
22	1041.0
23	1596.0
24	2540.0
25	3483.0
26	4853.0
27	6647.0
28	8597.0
29	11729.0
30	15761.0
31	22448.0
32	33141.0
33	51003.0
34	87582.0
35	95153.0
36	138020.0
37	176732.0
38	200595.0
39	27002.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.59890463193204	0.3408411311608833	0.03619750799267956	0.024056728914389522
8	99.19870858083286	0.5495950825348148	0.1804254668579214	0.07127086977440635
9	97.86344771146315	1.1205489430777325	0.38783044277870954	0.6281729026804141
10	62.50477762139654	24.54314697986879	4.865642044866923	8.086433353867738
11	36.04383720564231	25.266647481237996	18.71028953509809	19.979225778021593
12	31.324334168199254	15.899024690747376	27.61566340364493	25.16097773740844
13	24.53314207859131	16.34958249209725	31.027671983128812	28.089603446182625
14	24.66815203852674	17.523865624058526	30.425242028679218	27.382740308735514
15	26.02971792923275	18.893525367483395	29.27939979585505	25.79735690742881
16	29.723999622286872	27.101366512134035	22.5811745978929	20.593459267686193
17	32.37248809529162	19.457284692276218	22.841302031107375	25.328925181324784
18	29.767054422166368	21.921862845169095	23.683399957732103	24.62768277493244
19	33.750803764540834	19.560368899820585	22.42098376283213	24.267843572806452
20	31.130194117567704	20.62302431303425	23.34323331429779	24.90354825510025
21	32.69410632624521	18.892850879756825	23.679240616751578	24.733802177246382
22	31.0995049260087	19.410182966037297	24.998201366062474	24.492110741891533
23	31.917883367582323	19.06675629859122	24.795630218848785	24.219730114977676
24	32.78145248683625	18.198353350630196	24.35552697726077	24.664667185272783
25	29.432845753650106	18.51985916696269	23.814138162065912	28.233156917321296
26	26.393941301581446	19.535637683179623	24.957619687847078	29.112801327391846
27	27.030432886222915	19.33250446286046	24.293698935658366	29.343363715258263
28	28.804223192485306	21.200947880085074	22.93258270343674	27.062246223992876
29	30.650183685490866	21.263787653277337	22.232239614013157	25.85378904721864
30	29.796057394408948	18.735920068707816	23.828976892050488	27.63904564483275
31	32.09830883444024	19.31552985507507	23.293770881015867	25.29239042946882
32	33.293613500546336	19.995413483459313	22.01415524908832	24.696817766906033
33	33.32553925293739	19.214019452226033	23.676205421982004	23.784235872854566
34	29.940847426379662	22.007297957201505	25.94439523182143	22.107459384597398
35	29.215435876451835	24.741558786101955	23.842916305066304	22.200089032379907
36	31.180331038576202	24.731329055582286	21.101236111173563	22.98710379466795
37	30.02482114833784	24.956832785499415	21.94929201271634	23.069054053446408
38	32.23759054997729	24.24670962404054	21.173518712537827	22.34218111344434
39	31.894163882531217	22.159844598027796	20.44642094329357	25.499570576147416
40	28.416505164327692	22.215265006227767	22.21481534774339	27.153414481701148
41	26.981082867562083	22.664136588261215	23.813014015854957	26.54176652832174
42	25.298123575144675	21.715919259322543	22.298451825838274	30.687505339694503
43	25.645821998192375	19.850061378383117	26.5499727956617	27.954143827762813
44	26.511414580626013	19.99406450800617	25.032937483980916	28.4615834273869
45	27.63814632786399	20.025765431155037	24.828342873587513	27.507745367393465
46	27.563390604835625	21.300209990512204	23.829763794398154	27.30663561025401
47	26.43890715001956	21.54763457154291	24.631392457428582	27.382065821008943
48	27.9568417786691	21.17700356579178	25.37737588301685	25.48877877252227
49	28.468665548515904	20.462721063352383	24.918724228948115	26.149889159183598
50	25.844571048288824	20.037006893264568	25.41964378054867	28.698778277897937
51	25.24202867921813	20.819974729193177	26.421370469128696	27.516626122459993
52	26.51726014092297	20.661694942691025	25.08993169687622	27.731113219509783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	20.0
12	40.0
13	99.5
14	252.5
15	346.0
16	745.5
17	1145.0
18	1084.0
19	1023.0
20	1160.5
21	1298.0
22	1423.0
23	1548.0
24	1683.0
25	1818.0
26	2038.0
27	2258.0
28	2461.5
29	2665.0
30	3598.0
31	4531.0
32	5482.0
33	6433.0
34	9726.0
35	13019.0
36	11555.0
37	10091.0
38	9841.0
39	12237.0
40	14883.0
41	14883.0
42	14883.0
43	16852.0
44	18821.0
45	19553.0
46	20285.0
47	23061.0
48	25837.0
49	26109.0
50	26381.0
51	28547.0
52	30713.0
53	32586.0
54	34459.0
55	38874.0
56	43289.0
57	48061.0
58	52833.0
59	62968.5
60	73104.0
61	79278.0
62	85452.0
63	84010.5
64	85393.0
65	88217.0
66	76849.0
67	65481.0
68	58048.5
69	50616.0
70	40203.0
71	29790.0
72	26633.0
73	23476.0
74	21899.0
75	20322.0
76	18696.5
77	17071.0
78	13858.5
79	10646.0
80	6769.5
81	2893.0
82	1915.0
83	937.0
84	721.5
85	506.0
86	302.0
87	98.0
88	61.5
89	15.5
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	889564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.257288642250394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.07757927761132	7.663689599937548
2	11.090708617624998	2.4970261631139694
3	4.584766259099639	1.5483611140780558
4	2.5398359250255393	1.1436666444787813
5	1.5718979390984908	0.8847654408295121
6	1.0459320809940997	0.706461560156412
7	0.7919485736041941	0.6240635578016627
8	0.5929614951327526	0.5340110963559799
9	0.5179663650568838	0.5247807190580321
>10	5.021673909875798	13.01449429329308
>50	2.0568664342123686	16.914328701651183
>100	1.9968703301517259	40.51201339485802
>500	0.07699500021115818	5.921174471018991
>1k	0.03299785723335351	6.870439980203112
>5k	9.99935067677379E-4	0.6407232631656609
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5692	0.6398640232743231	No Hit
TCGGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4584	0.515308623100755	No Hit
TCGGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4030	0.4530309230139709	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3366	0.37838761460670617	No Hit
TCGGGTGGGATATCATAATTTATTTTAAAGAGAAAGTGATTCAGTGGGGAAA	3330	0.3743406882472762	No Hit
TCGGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2803	0.31509818293006464	No Hit
TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2777	0.3121754027815874	No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2553	0.2869945276562451	No Hit
TCGGGTGGGACTTAAAGAGGTGTGATTTTTGCTAAGTCACTGTTTTAAAGGA	2437	0.2739544316091928	No Hit
TCGGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2114	0.237644508995418	No Hit
TCGGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1938	0.21785953568264904	No Hit
TCGGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1858	0.20886636599502678	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1827	0.20538151274107314	No Hit
TCGGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1779	0.1999856109284998	No Hit
TCGGGTGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA	1744	0.19605109919016506	No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTG	1712	0.19245383131511618	No Hit
TCGGGTGGGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTG	1703	0.19144209972525866	No Hit
TCGGGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1416	0.15917910347091385	No Hit
TCGGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1415	0.15906668884981856	No Hit
TCGGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1334	0.14996110454110104	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1298	0.14591417818167102	No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1286	0.1445652027285277	No Hit
TCGGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1272	0.1429913980331938	No Hit
TCGGGTGGCTATTAATTATTCGGTCTGCATTCCCAGTGGGCAGTGATGGCAT	1265	0.14220449568552684	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1260	0.14164242258005044	No Hit
TCGGGTGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTG	1227	0.13793274008390627	No Hit
TCGGGTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	1194	0.1342230575877621	No Hit
TCGGGTGGGAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGAC	1163	0.13073820433380848	No Hit
TCGGGTGGGAATTAGAAAGTATCTTGTTATATACACAGACACTTTTATGGTC	1093	0.12286918085713901	No Hit
TCGGGTGGGAATGGGAAGGCGGGGAGGTGCTTGGAGATTTCCTAAACCACGC	1090	0.12253193699385317	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1083	0.12174503464618622	No Hit
TCGGGTGGGAAGTCTGTGATGTATTATTAATAATGCCTTATATATTGTTTGT	1043	0.1172484498023751	No Hit
TCGGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1028	0.11556223048594594	No Hit
TCGGGTGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	1013	0.11387601116951676	No Hit
TCGGGTGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTC	999	0.11230220647418286	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT	964	0.10836769473584812	No Hit
TCGGGTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	962	0.10814286549365756	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC	946	0.10634423155613311	No Hit
TCGGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	940	0.10566974382956146	No Hit
TCGGGTGGGGCCGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCAACTG	934	0.10499525610298976	No Hit
TCGGGTGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG	901	0.10128557360684559	No Hit
TCGGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	899	0.10106074436465505	No Hit
TCGGGTGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCC	897	0.1008359151224645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.1241462109527814E-4	0.0	0.0	0.0	0.0
8	2.2482924219055628E-4	0.0	0.0	0.0	0.0
9	2.2482924219055628E-4	0.0	0.0	0.0	0.0
10	0.0017986339375244502	0.0	0.0	0.0	0.0
11	0.0070821211290025225	0.0	0.0	0.0	0.0
12	0.00966765741419392	0.0	0.0	0.0	0.0
13	0.011353876730623092	0.0	0.0	0.0	0.0
14	0.01585046157443422	0.0	0.0	0.0	0.0
15	0.02034704641824534	0.0	0.0	0.0	0.0
16	0.03023953307462982	0.0	0.0	0.0	0.0
17	0.03361197170748816	0.0	0.0	0.0	0.0
18	0.03788372730910873	0.0	0.0	0.0	0.0
19	0.04282997063730097	0.0	0.0	0.0	0.0
20	0.04743897010220737	0.0	0.0	0.0	0.0
21	0.05171072570382794	0.0	0.0	0.0	0.0
22	0.055645237442162675	0.0	0.0	0.0	0.0
23	0.06036665152816436	0.0	0.0	0.0	0.0
24	0.0643011632664991	0.0	0.0	0.0	0.0
25	0.06857291886811967	0.0	0.0	0.0	0.0
26	0.07138328439550162	0.0	0.0	0.0	0.0
27	0.07453089378616941	0.0	0.0	0.0	0.0
28	0.0791398932510758	0.0	0.0	0.0	0.0
29	0.08273716112612471	0.0	0.0	0.0	0.0
30	0.08599718513788777	0.0	0.0	0.0	0.0
31	0.09217998929812807	0.0	0.0	0.0	0.0
32	0.09870003732165421	0.0	0.0	0.0	0.0
33	0.10398352451313228	0.0	0.0	0.0	0.0
34	0.1090421824624198	0.0	0.0	0.0	0.0
35	0.11736086442347038	0.0	0.0	0.0	0.0
36	0.12433057093137762	0.0	0.0	0.0	0.0
37	0.1296140581228557	0.0	0.0	0.0	0.0
38	0.1346727160721432	0.0	0.0	0.0	0.0
39	0.1393941301581449	0.0	0.0	0.0	0.0
40	0.1454645196972899	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	6.310681E-4	46.0	33
AACGTAG	25	3.4165292E-5	46.0	16
TAATACG	25	3.4165292E-5	46.0	29
ACTATCG	25	3.4165292E-5	46.0	21
ACTATCC	45	3.092282E-10	46.0	32
TGATACA	30	1.8609244E-6	46.0	42
GGTACGC	30	1.8609244E-6	46.0	9
GTACCGA	20	6.310681E-4	46.0	28
ACACGTT	25	3.4165292E-5	46.0	44
CAAACCG	25	3.4165292E-5	46.0	13
CATATGA	185	0.0	46.0	31
AATTTCG	30	1.8609244E-6	46.0	39
CATATCG	20	6.310681E-4	46.0	17
CGTATAC	20	6.310681E-4	46.0	34
GGTAAGT	20	6.310681E-4	46.0	13
ACGTTTC	20	6.310681E-4	46.0	19
ACGTTTA	30	1.8609244E-6	46.0	22
ACCATTA	25	3.4165292E-5	46.0	25
ACGCATA	20	6.310681E-4	46.0	10
AACCCTA	75	0.0	46.0	45
>>END_MODULE