FastQCFastQC Report
Fri 17 Jun 2016
SRR1527238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865975
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC56340.6505961488495626No Hit
TCGGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG42930.49574179393169543No Hit
TCGGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT42780.4940096423106903No Hit
TCGGGTGGGATATCATAATTTATTTTAAAGAGAAAGTGATTCAGTGGGGAAA32830.379110251450677No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT32670.3772626230549381No Hit
TCGGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG28710.3315338202604001No Hit
TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC26850.3100551401599353No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC25880.2988538930107682No Hit
TCGGGTGGGACTTAAAGAGGTGTGATTTTTGCTAAGTCACTGTTTTAAAGGA21660.2501226940731545No Hit
TCGGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19710.22760472300008663No Hit
TCGGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT19260.22240826813707096No Hit
TCGGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG18600.21478680100464795No Hit
TCGGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA18280.2110915442131701No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT17500.20208435578394296No Hit
TCGGGTGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA17300.19977482028926932No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTG16410.18949738733797164No Hit
TCGGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC14110.16293772914922486No Hit
TCGGGTGGGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTG13970.16132105430295332No Hit
TCGGGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT13840.15981985623141545No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG12950.14954242328011777No Hit
TCGGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC12930.14931146973065043No Hit
TCGGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA12130.14007332775195588No Hit
TCGGGTGGCTATTAATTATTCGGTCTGCATTCCCAGTGGGCAGTGATGGCAT12020.13880308322988538No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT12010.1386876064551517No Hit
TCGGGTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT11860.13695545483414648No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC11670.13476139611420654No Hit
TCGGGTGGGAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGAC11150.12875660382805507No Hit
TCGGGTGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTG11110.12829469672912036No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT10900.12586968445971305No Hit
TCGGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT10890.12575420768497936No Hit
TCGGGTGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC10650.122982765091371No Hit
TCGGGTGGGAAGTCTGTGATGTATTATTAATAATGCCTTATATATTGTTTGT10230.11813274055255636No Hit
TCGGGTGGGAATGGGAAGGCGGGGAGGTGCTTGGAGATTTCCTAAACCACGC10050.11605415860735009No Hit
TCGGGTGGGGCCGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCAACTG9820.1133981927884754No Hit
TCGGGTGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTC9750.11258985536533965No Hit
TCGGGTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG9330.10773983082652501No Hit
TCGGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA9090.10496838823291665No Hit
TCGGGTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC9030.10427552758451455No Hit
TCGGGTGGGAATTAGAAAGTATCTTGTTATATACACAGACACTTTTATGGTC9020.10416005080978089No Hit
TCGGGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC8810.10173503854037357No Hit
TCGGGTGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG8800.10161956176563988No Hit
TCGGGTGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACA8780.10138860821617252No Hit
TCGGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT8750.10104217789197148No Hit
TCGGGTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA8710.10058027079303675No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT8700.10046479401830306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG206.310587E-446.00000444
ATTAGGC206.310587E-446.00000416
CGAAATT206.310587E-446.00000433
CATGATA206.310587E-446.00000436
GACAACG206.310587E-446.00000436
CGACCGT206.310587E-446.00000423
GGATCGA800.046.0000048
GACCCGT206.310587E-446.00000434
TCTACGA206.310587E-446.00000432
TACGATG206.310587E-446.00000421
CTACGAA206.310587E-446.00000433
GTGTCGA206.310587E-446.00000442
ACGGACC206.310587E-446.00000413
AACGATT206.310587E-446.00000428
TATAGGG206.310587E-446.00000434
GTATACG206.310587E-446.00000446
AACTAAC206.310587E-446.00000415
TATAATC206.310587E-446.00000425
AGTACGA206.310587E-446.00000419
CCCATAT206.310587E-446.00000433
Produced by FastQC (version 0.11.5)