##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865975
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.573733652819076	31.0	31.0	33.0	30.0	33.0
2	32.24042149022778	33.0	31.0	34.0	31.0	34.0
3	32.718975721008114	34.0	31.0	34.0	31.0	34.0
4	36.42757931810964	37.0	37.0	37.0	35.0	37.0
5	36.40951990530905	37.0	37.0	37.0	35.0	37.0
6	31.470593261930194	33.0	32.0	35.0	28.0	35.0
7	34.72606137590577	35.0	35.0	35.0	32.0	35.0
8	36.0712179912815	37.0	35.0	37.0	35.0	37.0
9	38.634522936574385	39.0	39.0	39.0	38.0	39.0
10	38.13928693091602	39.0	39.0	39.0	37.0	39.0
11	37.72747827593175	39.0	37.0	39.0	35.0	39.0
12	37.185399116602674	39.0	37.0	39.0	34.0	39.0
13	37.01826727099512	39.0	37.0	39.0	34.0	39.0
14	37.881255232541356	40.0	37.0	40.0	34.0	40.0
15	37.939303097664485	40.0	37.0	40.0	34.0	40.0
16	37.99313375097434	40.0	37.0	40.0	34.0	40.0
17	37.90914056410404	40.0	37.0	40.0	34.0	40.0
18	37.87977366552152	40.0	37.0	40.0	34.0	40.0
19	37.86504460290424	40.0	37.0	40.0	34.0	40.0
20	37.73538958976876	40.0	37.0	40.0	33.0	40.0
21	33.4382851698952	39.0	34.0	40.0	10.0	40.0
22	34.047836253933426	39.0	33.0	40.0	16.0	40.0
23	36.12773117006842	39.0	34.0	40.0	30.0	40.0
24	36.98225121972343	39.0	35.0	40.0	32.0	40.0
25	37.29588498513237	39.0	36.0	40.0	33.0	40.0
26	37.335533935737175	39.0	36.0	40.0	33.0	40.0
27	37.28917924882358	39.0	35.0	40.0	33.0	40.0
28	37.15394439793297	39.0	35.0	40.0	33.0	40.0
29	37.18578711856578	39.0	35.0	40.0	33.0	40.0
30	37.08756950258379	39.0	35.0	40.0	33.0	40.0
31	36.94731141199226	39.0	35.0	40.0	32.0	40.0
32	36.81003493172436	39.0	35.0	40.0	32.0	40.0
33	36.460428996218134	38.0	35.0	40.0	31.0	40.0
34	36.24265827535437	38.0	35.0	40.0	31.0	40.0
35	36.15940413984237	38.0	35.0	40.0	31.0	40.0
36	36.03189699471694	38.0	35.0	40.0	31.0	40.0
37	35.90442564739167	38.0	34.0	40.0	30.0	40.0
38	35.85305580415139	37.0	34.0	40.0	30.0	40.0
39	35.66051791333468	37.0	34.0	40.0	30.0	40.0
40	30.84552094460002	35.0	30.0	39.0	8.0	40.0
41	31.64471260717688	35.0	30.0	39.0	12.0	40.0
42	33.32438465313664	35.0	30.0	39.0	23.0	40.0
43	34.12853488842057	35.0	32.0	39.0	27.0	40.0
44	35.06381939432432	36.0	34.0	39.0	30.0	40.0
45	35.335649412511906	36.0	34.0	40.0	30.0	40.0
46	35.36438234360114	36.0	34.0	40.0	30.0	40.0
47	35.268319524235686	35.0	34.0	40.0	30.0	40.0
48	35.12630156759722	35.0	34.0	39.0	30.0	40.0
49	34.99122145558474	35.0	34.0	39.0	30.0	40.0
50	34.89659285776148	35.0	34.0	39.0	30.0	40.0
51	34.695727936718725	35.0	34.0	39.0	30.0	40.0
52	34.2515141892087	35.0	33.0	38.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	9.0
16	32.0
17	63.0
18	143.0
19	280.0
20	484.0
21	705.0
22	1123.0
23	1679.0
24	2618.0
25	3675.0
26	4950.0
27	6963.0
28	9363.0
29	12865.0
30	18139.0
31	26577.0
32	36578.0
33	51498.0
34	96860.0
35	100789.0
36	121024.0
37	142343.0
38	199382.0
39	27833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.59144317099224	0.3423886370853662	0.04110973180519068	0.025058460117208924
8	99.20043881174398	0.5463206212650481	0.17610208146886458	0.07713848552209937
9	97.80732700135685	1.1178151794220388	0.4328069517018389	0.6420508675192702
10	62.542452149311465	24.623112676462945	4.784318253991167	8.050116920234418
11	36.074597996477955	25.253153959409914	18.709547042351108	19.962701001761022
12	31.402754121077397	15.962354571436821	27.547561996593434	25.087329310892347
13	24.682121308351856	16.348624382920985	31.00251161985046	27.9667426888767
14	24.675077225093105	17.55385548081642	30.39810618089437	27.37296111319611
15	26.002713704206244	18.766938999393748	29.283755304714337	25.946591991685676
16	31.191547100089494	19.286353532145846	25.74704812494587	23.775051242818787
17	32.38476861341263	19.476774733681687	22.719478044978203	25.418978607927478
18	29.738733797165047	21.868067784866767	23.73682843038194	24.656369987586245
19	33.588267559687054	19.654839920321027	22.43863852882589	24.318253991166028
20	31.21025433759635	20.624960304858686	23.32711683362684	24.837668523918126
21	36.69389993937469	18.520627038886804	22.632870463927944	22.15260255781056
22	31.165911256098617	19.38739571003782	24.995640751753804	24.45105228210976
23	31.853806403187157	19.077109616328418	24.94690955281619	24.122174427668234
24	32.790207569502584	18.26265192413176	24.334882646727678	24.61225785963798
25	29.435838217038597	18.51577701434799	23.883137503969515	28.1652472646439
26	26.492912612950718	19.449060307745604	25.00741938277664	29.050607696527038
27	27.127342013337568	19.418689915990647	24.210629637114238	29.243338433557554
28	28.668264095383815	21.331793643003554	23.010941424406017	26.989000837206618
29	30.51138889690811	21.177863102283553	22.32166055602067	25.989087444787668
30	29.79912815035076	18.746153179941686	23.844568261208465	27.61015040849909
31	32.159704379456684	19.320650134241752	23.295360720575076	25.224284765726495
32	33.17335950806894	20.063512226103526	22.058950893501546	24.70417737232599
33	33.36100926701117	19.234504460290424	23.536245272669536	23.86824100002887
34	29.961026588527385	22.049366321198647	25.987124339617196	22.002482750656775
35	29.249112272294237	24.772193192644128	23.818932417217585	22.159762117844046
36	31.228268714454803	24.748520453823726	21.06954588758336	22.95366494413811
37	29.891163139813504	24.97577874649961	21.94693842200987	23.186119691677014
38	32.204162937729144	24.37691619273074	21.103727012904532	22.315193856635585
39	31.683362683680244	22.189786079274807	20.459943993764256	25.666907243280697
40	28.6400877623488	26.878258610237015	20.45636421374751	24.025289413666677
41	27.001241375328387	22.707814890730102	23.783481047374348	26.507462686567163
42	25.36874621091833	21.734461156499897	22.271659112560986	30.625133520020785
43	25.534339905886426	19.927249631917782	26.52259014405728	28.015820318138513
44	26.430324201045064	20.034412078870638	25.045295764889286	28.489967955195013
45	27.53485955137273	19.96200814111262	24.994716937555932	27.508415369958716
46	27.61453852593897	21.311469730650423	23.736597476832472	27.337394266578137
47	26.31992840439967	21.571754380900142	24.635584168134184	27.472733046566013
48	27.922053177054767	21.137446231126763	25.406738069805712	25.533762522012758
49	28.47830480094691	20.50370969138832	24.97104419873553	26.046941308929238
50	25.740234995236584	19.97309391148705	25.51690291290164	28.769768180374722
51	25.323017408123793	20.858454343370187	26.370738185282484	27.447790063223533
52	26.42270273391264	20.757758595802418	25.191142931377925	27.628395738907013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	15.0
12	30.0
13	100.0
14	236.5
15	303.0
16	695.5
17	1088.0
18	1035.5
19	983.0
20	1116.5
21	1250.0
22	1410.0
23	1570.0
24	1737.0
25	1904.0
26	2065.5
27	2227.0
28	2370.0
29	2513.0
30	3431.0
31	4349.0
32	5268.5
33	6188.0
34	9222.5
35	12257.0
36	11050.0
37	9843.0
38	9690.0
39	11926.0
40	14315.0
41	14287.0
42	14259.0
43	16320.0
44	18381.0
45	18948.5
46	19516.0
47	22446.0
48	25376.0
49	25581.5
50	25787.0
51	27921.5
52	30056.0
53	31759.0
54	33462.0
55	37882.0
56	42302.0
57	46790.0
58	51278.0
59	60429.0
60	69580.0
61	76366.5
62	83153.0
63	81445.0
64	83624.0
65	87511.0
66	75898.5
67	64286.0
68	56777.5
69	49269.0
70	39169.0
71	29069.0
72	25781.0
73	22493.0
74	20908.5
75	19324.0
76	18221.0
77	17118.0
78	13970.5
79	10823.0
80	6962.0
81	3101.0
82	2042.0
83	983.0
84	722.5
85	462.0
86	282.5
87	103.0
88	58.0
89	8.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	865975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.327230161699873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.87714930258521	8.912871613139194
2	12.200319997608243	3.2519294530902942
3	5.673074693058841	2.2681911647673014
4	3.1318989655448326	1.669581534280229
5	1.914492404691634	1.275744046007583
6	1.224533244356092	0.9791781832912027
7	0.84997064713339	0.7929428111524974
8	0.5979940914216862	0.6375683913370727
9	0.47977504346565136	0.5754665187095668
>10	3.5827789639029466	11.19169178618504
>50	1.727534540372078	16.68599149927687
>100	1.6515989561798987	38.99002410792197
>500	0.06040330560741532	5.406668810198925
>1k	0.027612939706247005	6.714233033025355
>5k	8.629043658202189E-4	0.6479170476168958
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5634	0.6505961488495626	No Hit
TCGGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4293	0.49574179393169543	No Hit
TCGGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4278	0.4940096423106903	No Hit
TCGGGTGGGATATCATAATTTATTTTAAAGAGAAAGTGATTCAGTGGGGAAA	3283	0.379110251450677	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3267	0.3772626230549381	No Hit
TCGGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2871	0.3315338202604001	No Hit
TCGGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2685	0.3100551401599353	No Hit
TCGGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2588	0.2988538930107682	No Hit
TCGGGTGGGACTTAAAGAGGTGTGATTTTTGCTAAGTCACTGTTTTAAAGGA	2166	0.2501226940731545	No Hit
TCGGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1971	0.22760472300008663	No Hit
TCGGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1926	0.22240826813707096	No Hit
TCGGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1860	0.21478680100464795	No Hit
TCGGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1828	0.2110915442131701	No Hit
TCGGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1750	0.20208435578394296	No Hit
TCGGGTGGGATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA	1730	0.19977482028926932	No Hit
TCGGGTGGGTATTGTCAATGTTAAGGTTCCTAAACATACTTGTGAGTAGGTG	1641	0.18949738733797164	No Hit
TCGGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1411	0.16293772914922486	No Hit
TCGGGTGGGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAATTG	1397	0.16132105430295332	No Hit
TCGGGTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1384	0.15981985623141545	No Hit
TCGGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1295	0.14954242328011777	No Hit
TCGGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1293	0.14931146973065043	No Hit
TCGGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1213	0.14007332775195588	No Hit
TCGGGTGGCTATTAATTATTCGGTCTGCATTCCCAGTGGGCAGTGATGGCAT	1202	0.13880308322988538	No Hit
TCGGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1201	0.1386876064551517	No Hit
TCGGGTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	1186	0.13695545483414648	No Hit
TCGGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1167	0.13476139611420654	No Hit
TCGGGTGGGAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGAC	1115	0.12875660382805507	No Hit
TCGGGTGGGACCTTCAAGGCTTTTGACAAGCATATGAATTTGATCCTCTGTG	1111	0.12829469672912036	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1090	0.12586968445971305	No Hit
TCGGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1089	0.12575420768497936	No Hit
TCGGGTGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	1065	0.122982765091371	No Hit
TCGGGTGGGAAGTCTGTGATGTATTATTAATAATGCCTTATATATTGTTTGT	1023	0.11813274055255636	No Hit
TCGGGTGGGAATGGGAAGGCGGGGAGGTGCTTGGAGATTTCCTAAACCACGC	1005	0.11605415860735009	No Hit
TCGGGTGGGGCCGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGCACCCAACTG	982	0.1133981927884754	No Hit
TCGGGTGGGGAGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTC	975	0.11258985536533965	No Hit
TCGGGTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	933	0.10773983082652501	No Hit
TCGGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	909	0.10496838823291665	No Hit
TCGGGTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	903	0.10427552758451455	No Hit
TCGGGTGGGAATTAGAAAGTATCTTGTTATATACACAGACACTTTTATGGTC	902	0.10416005080978089	No Hit
TCGGGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	881	0.10173503854037357	No Hit
TCGGGTGGGGGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAG	880	0.10161956176563988	No Hit
TCGGGTGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACA	878	0.10138860821617252	No Hit
TCGGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	875	0.10104217789197148	No Hit
TCGGGTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	871	0.10058027079303675	No Hit
TCGGGTGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTCT	870	0.10046479401830306	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.464303242010451E-4	0.0	0.0	0.0	0.0
9	4.6190709893472674E-4	0.0	0.0	0.0	0.0
10	0.0025404890441409972	0.0	0.0	0.0	0.0
11	0.007852420681890356	0.0	0.0	2.3095354946736337E-4	0.0
12	0.011432200698634487	0.0	0.0	2.3095354946736337E-4	0.0
13	0.013626259418574439	0.0	0.0	2.3095354946736337E-4	0.0
14	0.01789890008372066	0.0	0.0	2.3095354946736337E-4	0.0
15	0.024019169144605793	0.0	0.0	2.3095354946736337E-4	0.0
16	0.0327954040243656	0.0	0.0	2.3095354946736337E-4	0.0
17	0.03602875371690869	0.0	0.0	2.3095354946736337E-4	0.0
18	0.03926210340945177	0.0	0.0	2.3095354946736337E-4	0.0
19	0.04318831375039695	0.0	0.0	2.3095354946736337E-4	0.0
20	0.048269291838678945	0.0	0.0	2.3095354946736337E-4	0.0
21	0.05150264153122203	0.0	0.0	2.3095354946736337E-4	0.0
22	0.055659805421634574	0.0	0.0	2.3095354946736337E-4	0.0
23	0.05912410866364502	0.0	0.0	2.3095354946736337E-4	0.0
24	0.06293484222985651	0.0	0.0	2.3095354946736337E-4	0.0
25	0.06674557579606802	0.0	0.0	2.3095354946736337E-4	0.0
26	0.07055630936227951	0.0	0.0	2.3095354946736337E-4	0.0
27	0.074367042928491	0.0	0.0	2.3095354946736337E-4	0.0
28	0.07875516036837091	0.0	0.0	2.3095354946736337E-4	0.0
29	0.08302780103351713	0.0	0.0	2.3095354946736337E-4	0.0
30	0.08753139524813072	0.0	0.0	2.3095354946736337E-4	0.0
31	0.09180403591327695	0.0	0.0	2.3095354946736337E-4	0.0
32	0.10127313144143885	0.0	0.0	2.3095354946736337E-4	0.0
33	0.10577672565605242	0.0	0.0	2.3095354946736337E-4	0.0
34	0.11074222696960073	0.0	0.0	2.3095354946736337E-4	0.0
35	0.11686249603048587	0.0	0.0	2.3095354946736337E-4	0.0
36	0.12506134703657726	0.0	0.0	2.3095354946736337E-4	0.0
37	0.13083518577326136	0.0	0.0	2.3095354946736337E-4	0.0
38	0.1367245012846791	0.0	0.0	2.3095354946736337E-4	0.0
39	0.14203643292242848	0.0	0.0	2.3095354946736337E-4	0.0
40	0.14988885360431883	0.0	0.0	2.3095354946736337E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTG	20	6.310587E-4	46.000004	44
ATTAGGC	20	6.310587E-4	46.000004	16
CGAAATT	20	6.310587E-4	46.000004	33
CATGATA	20	6.310587E-4	46.000004	36
GACAACG	20	6.310587E-4	46.000004	36
CGACCGT	20	6.310587E-4	46.000004	23
GGATCGA	80	0.0	46.000004	8
GACCCGT	20	6.310587E-4	46.000004	34
TCTACGA	20	6.310587E-4	46.000004	32
TACGATG	20	6.310587E-4	46.000004	21
CTACGAA	20	6.310587E-4	46.000004	33
GTGTCGA	20	6.310587E-4	46.000004	42
ACGGACC	20	6.310587E-4	46.000004	13
AACGATT	20	6.310587E-4	46.000004	28
TATAGGG	20	6.310587E-4	46.000004	34
GTATACG	20	6.310587E-4	46.000004	46
AACTAAC	20	6.310587E-4	46.000004	15
TATAATC	20	6.310587E-4	46.000004	25
AGTACGA	20	6.310587E-4	46.000004	19
CCCATAT	20	6.310587E-4	46.000004	33
>>END_MODULE