FastQCFastQC Report
Fri 17 Jun 2016
SRR1527234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869177
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC81610.938934187167861No Hit
AAAGTTGGGGCAGAGTGCAGTGCTAAAGTTGGGGTGTAATTTATAACAAG55910.6432521799357324No Hit
AAAGTTGGGCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCA46450.5344135889467853No Hit
AAAGTTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA35180.40475070095043936No Hit
AAAGTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA34280.39439607812908073No Hit
AAAGTTGGGGAAGCAGTCCTATCCAGAGTGCACTGCTAAAGTTGGGACTA32030.36850952107568424No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA25150.2893541821746318No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22850.26289236829782653No Hit
AAAGTTGGGAAGAACAATAGAATATAAAGAAGTGTGAAAACCCACCCTAT20590.23689075987974834No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC19800.22780170206988912No Hit
AAAGTTGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAAC19410.22331469884730037No Hit
AAAGTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC19290.22193408247111923No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGA17700.20364091548671906No Hit
AAAGTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG17070.19639267951176803No Hit
AAAGTTGGGATTCTGAGTGCAGCTTCTCACTTAAGAAGGTTACATGGATT16770.19294113857131515No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC16600.1909852653717252No Hit
AAAGTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA15200.17487807431627853No Hit
AAAGTTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA14390.16555891377705576No Hit
AAAGTTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC14200.16337293784810228No Hit
AAAGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA13840.15923108871955885No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAAC13700.15762036961401418No Hit
AAAGTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT12990.14945172272160906No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11560.13299937757211708No Hit
AAAGTTGGGGACTGTGCCTGAGATGGAGCCTCTCCAGGATAGCCACAGCA11320.1302381448197548No Hit
AAAGTTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG11100.127707014796756No Hit
AAAGTTGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACAC11080.12747691206739248No Hit
AAAGTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC11060.12724680933802898No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG11010.12667155251462014No Hit
AAAGTTGGGAGCCACGCTCTCGTCAGCACTAGCATCTCTTGTGAGTGCTC11010.12667155251462014No Hit
AAAGTTGGACCGACAGGATTGGTCTCTCCAGAGGCGTCTCTCCTTAGCTT10580.12172434383330437No Hit
AAAGTTGGGGGTCCGCAAAGGTAATGAGCAGCGCGCTTTCACCCGCTGCT10400.11965341926903265No Hit
AAAGTTGGGTTGTTTTTGTATTTTTCGAGTCCTGTGTAAGTTTTGAAAGC10320.11873300835157856No Hit
AAAGTTGGGCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGA9730.11194497783535459No Hit
AAAGTTGGGCAATCAAGTCTGTGATGTATTATTAATAATGCCTTATATAT9640.11090951555321875No Hit
AAAGTTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT9180.10561715277785767No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGTGCAGTGCTAAAGTTGGGATAG9040.104006433672313No Hit
AAAGTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT8770.10090004682590542No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8770.10090004682590542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC302.527002E-644.00000430
AGTCATA302.527002E-644.00000410
CCTAGAC302.527002E-644.00000442
CTTCACG302.527002E-644.00000435
CGCATAA302.527002E-644.00000443
CGTCTAG302.527002E-644.00000444
CACGACC302.527002E-644.00000440
TAATTGG302.527002E-644.00000438
GTGTCGA302.527002E-644.00000421
ACTACTA302.527002E-644.00000435
ACTACGC302.527002E-644.00000412
GCGATAC302.527002E-644.00000426
CACAACG302.527002E-644.00000422
GGTTAAC302.527002E-644.0000049
TATCGAA302.527002E-644.00000410
CGATACA302.527002E-644.00000427
AATAAGA650.044.00000411
TAGTAGG302.527002E-644.00000438
TACAAAC302.527002E-644.00000433
TCACGAC254.4419412E-544.042
Produced by FastQC (version 0.11.5)