##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527234_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	869177
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.934244693543434	31.0	31.0	33.0	31.0	34.0
2	32.73646909662819	34.0	31.0	34.0	31.0	34.0
3	33.0529109721035	34.0	31.0	34.0	31.0	34.0
4	36.428648019908486	37.0	37.0	37.0	35.0	37.0
5	34.75719214843467	35.0	35.0	35.0	33.0	37.0
6	35.530658312403574	35.0	35.0	37.0	35.0	37.0
7	36.234166343564084	37.0	35.0	37.0	35.0	37.0
8	36.6420176787927	37.0	37.0	37.0	35.0	37.0
9	38.73599738603299	39.0	39.0	39.0	39.0	39.0
10	38.23236233816588	39.0	39.0	39.0	37.0	39.0
11	37.93468303924287	39.0	38.0	39.0	35.0	39.0
12	37.61849197574257	39.0	37.0	39.0	35.0	39.0
13	37.42141934266553	39.0	37.0	39.0	35.0	39.0
14	38.13256793495456	40.0	38.0	40.0	34.0	40.0
15	38.16930958826568	40.0	38.0	40.0	34.0	40.0
16	38.0738583740711	40.0	38.0	40.0	34.0	40.0
17	38.236193548609776	40.0	38.0	40.0	35.0	40.0
18	38.20904948014041	40.0	38.0	40.0	35.0	40.0
19	38.164164491237116	40.0	38.0	40.0	34.0	40.0
20	37.943816967084956	40.0	38.0	40.0	34.0	40.0
21	38.07959368460049	40.0	38.0	40.0	34.0	40.0
22	37.969261726897976	40.0	38.0	40.0	34.0	40.0
23	38.0198486614349	40.0	38.0	40.0	34.0	40.0
24	38.01665943760592	40.0	38.0	40.0	34.0	40.0
25	37.96061331581485	40.0	38.0	40.0	34.0	40.0
26	37.798669315916094	40.0	37.0	40.0	34.0	40.0
27	37.68390557964603	40.0	37.0	40.0	34.0	40.0
28	37.52776246955453	40.0	37.0	40.0	33.0	40.0
29	37.730184991089274	40.0	37.0	40.0	34.0	40.0
30	37.833334292094705	40.0	37.0	40.0	34.0	40.0
31	37.779162357034295	40.0	37.0	40.0	34.0	40.0
32	37.049955302544824	40.0	36.0	40.0	32.0	40.0
33	37.2910465877491	40.0	36.0	40.0	33.0	40.0
34	37.33278837336929	40.0	36.0	40.0	33.0	40.0
35	37.24588317454327	40.0	36.0	40.0	33.0	40.0
36	37.047998278831585	40.0	36.0	40.0	32.0	40.0
37	36.85252716075092	40.0	35.0	40.0	31.0	40.0
38	36.64772077493997	40.0	35.0	40.0	31.0	40.0
39	36.56706516624347	40.0	35.0	40.0	31.0	40.0
40	36.40904901993495	39.0	35.0	40.0	30.0	40.0
41	36.279508086385164	39.0	35.0	40.0	30.0	40.0
42	36.2221227667092	39.0	35.0	40.0	30.0	40.0
43	36.153140269473305	39.0	35.0	40.0	30.0	40.0
44	35.9752190865612	39.0	35.0	40.0	30.0	40.0
45	35.79341722111837	38.0	35.0	40.0	29.0	40.0
46	35.5388373139188	38.0	35.0	40.0	29.0	40.0
47	35.39868404249077	38.0	35.0	40.0	28.0	40.0
48	35.265151977100174	37.0	35.0	40.0	28.0	40.0
49	35.01397989132248	37.0	34.0	40.0	27.0	40.0
50	34.55464652193972	36.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	1.0
14	4.0
15	6.0
16	28.0
17	45.0
18	109.0
19	212.0
20	336.0
21	603.0
22	923.0
23	1476.0
24	2180.0
25	3192.0
26	4421.0
27	6031.0
28	8250.0
29	10852.0
30	14804.0
31	18875.0
32	21672.0
33	26634.0
34	43853.0
35	54576.0
36	82345.0
37	143019.0
38	313430.0
39	111297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.60226743229515	0.3003991131840811	0.08571326668791282	0.01162018783285798
8	99.40806072871233	0.4617011264679116	0.05637516869406346	0.07386297612569132
9	97.4223892256698	1.582876675291684	0.450886298187826	0.5438478008506897
10	51.473635404526355	32.83186278514042	5.7094239723324485	9.985077838000775
11	30.73044960922804	26.29809578486315	23.864299216385156	19.10715538952365
12	31.988191127929067	17.86195446957294	26.886008258386955	23.26384614411104
13	25.160582942254567	17.323168928768247	29.37238330052452	28.143864828452664
14	25.252163828541253	19.759036421810517	30.031282466056968	24.95751728359126
15	25.6102036754309	20.67852692834716	28.2729524596256	25.438316936596344
16	30.638753671576673	21.20477187040154	25.83961609660633	22.316858361415452
17	31.50140880396053	22.766824248685825	21.822137493283876	23.90962945406977
18	30.300847813506337	20.444167298490413	23.889725567979823	25.365259320023426
19	28.403075553080676	21.458460129524827	23.729919222436855	26.408545094957642
20	26.626682482394266	22.742088205279245	24.10418131174663	26.52704800057986
21	27.683199164266885	21.622293272831655	28.336690915659297	22.357816647242164
22	28.80379945626725	21.453052715384782	25.09741974304428	24.645728085303684
23	26.629788869240674	23.344497150752954	25.773461561914317	24.252252418092056
24	26.45974295224103	24.582219731999352	25.285068518840237	23.672968796919385
25	29.715696572734895	22.085259964311067	23.465991391856893	24.73305207109714
26	28.837164352024963	21.880928740636257	22.94538396667192	26.33652294066686
27	27.721051063247188	21.536580006143744	24.469469394611224	26.272899535997844
28	32.32448626689386	21.490444408906356	20.67461518194798	25.51045414225181
29	29.385499156098238	22.555704994494793	23.79009108616542	24.26870476324155
30	31.543747706163416	19.678155312439237	23.75292949537321	25.025167486024134
31	29.01077686132974	22.004148752210426	23.47991260698339	25.50516177947645
32	31.53811018929401	21.585821990227537	22.682606649738776	24.193461170739678
33	31.38681764473749	21.216737212328443	22.659021119979013	24.73742402295505
34	27.88453905245997	24.39468600756808	24.756177395398176	22.964597544573774
35	28.40940337813817	24.133404358375795	25.175884773757247	22.28130748972879
36	29.44624627665021	23.10265918219189	22.237127765690992	25.213966775466908
37	26.41165148180405	26.10780082767952	22.84678494713965	24.63376274337678
38	30.486770818832063	24.526419820128698	21.479169375167544	23.50763998587169
39	28.046301271202527	23.95150815081393	21.65197652491955	26.350214053063993
40	28.126031866926986	23.549058477157125	23.63684266840931	24.68806698750657
41	26.757841038131474	24.194726735751175	23.070329748716315	25.977102477401036
42	25.022521304636452	22.51440155457404	24.84212076481545	27.620956375974053
43	27.11795180958539	23.994652412569593	25.075905137848792	23.811490639996226
44	29.27424448645098	22.232065505644997	23.941153527992572	24.552536479911456
45	25.964101673191998	22.57319280192642	23.919063665973674	27.543641858907908
46	27.677791750126847	23.060320279989003	24.919550333246278	24.342337636637875
47	26.70399699946041	25.279085847876786	23.352780849010042	24.664136303652764
48	26.850687489429653	25.474903270565143	22.01300770729092	25.66140153271428
49	27.060311075879827	26.466530982757252	21.37389737648373	25.099260564879188
50	25.12687289240281	27.339425686597778	21.770019225083036	25.763682195916367
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	4.0
2	8.0
3	5.5
4	3.0
5	72.0
6	141.0
7	796.0
8	1451.0
9	1415.0
10	1379.0
11	2058.5
12	2738.0
13	2382.0
14	2026.0
15	2180.0
16	2334.0
17	2391.0
18	2448.0
19	2289.5
20	2131.0
21	2762.0
22	3393.0
23	3662.0
24	3931.0
25	4758.5
26	5586.0
27	7452.5
28	9319.0
29	9677.0
30	10035.0
31	9591.5
32	9148.0
33	12810.0
34	16472.0
35	16690.5
36	16909.0
37	18108.5
38	19308.0
39	20967.5
40	22627.0
41	28337.5
42	34048.0
43	36227.0
44	38406.0
45	40742.0
46	43078.0
47	46778.0
48	50478.0
49	54697.0
50	58916.0
51	63083.0
52	67250.0
53	70947.0
54	74644.0
55	79973.0
56	85302.0
57	75918.0
58	66534.0
59	61727.5
60	56921.0
61	51065.5
62	45210.0
63	40861.5
64	36513.0
65	31825.0
66	27137.0
67	24258.0
68	21379.0
69	17840.0
70	14301.0
71	12923.0
72	11545.0
73	7812.5
74	4080.0
75	2638.0
76	1196.0
77	720.5
78	245.0
79	335.5
80	426.0
81	299.5
82	173.0
83	89.0
84	5.0
85	3.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	869177.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.516465090139373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.00899999999999	8.29289134675846
2	11.051999999999998	2.5455994435244067
3	4.366999999999999	1.508772091459159
4	2.2769999999999997	1.048919640409894
5	1.2500000000000204	0.7197790681337224
6	0.7549999999999999	0.5216958685833135
7	0.5459999999999999	0.4401592957451268
8	0.37799999999999995	0.34825790432581455
9	0.29499999999999993	0.3057621481432003
>10	2.7969999999999997	8.133273140609127
>50	1.9749999999999996	16.86344466684018
>100	2.1809999999999996	45.07003162421313
>500	0.086	6.434133881209965
>1k	0.03	6.183535600848533
>5k	0.002	1.5837442791959662
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	8161	0.938934187167861	No Hit
AAAGTTGGGGCAGAGTGCAGTGCTAAAGTTGGGGTGTAATTTATAACAAG	5591	0.6432521799357324	No Hit
AAAGTTGGGCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCA	4645	0.5344135889467853	No Hit
AAAGTTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA	3518	0.40475070095043936	No Hit
AAAGTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA	3428	0.39439607812908073	No Hit
AAAGTTGGGGAAGCAGTCCTATCCAGAGTGCACTGCTAAAGTTGGGACTA	3203	0.36850952107568424	No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2515	0.2893541821746318	No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	2285	0.26289236829782653	No Hit
AAAGTTGGGAAGAACAATAGAATATAAAGAAGTGTGAAAACCCACCCTAT	2059	0.23689075987974834	No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1980	0.22780170206988912	No Hit
AAAGTTGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAAC	1941	0.22331469884730037	No Hit
AAAGTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	1929	0.22193408247111923	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGA	1770	0.20364091548671906	No Hit
AAAGTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1707	0.19639267951176803	No Hit
AAAGTTGGGATTCTGAGTGCAGCTTCTCACTTAAGAAGGTTACATGGATT	1677	0.19294113857131515	No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1660	0.1909852653717252	No Hit
AAAGTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	1520	0.17487807431627853	No Hit
AAAGTTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	1439	0.16555891377705576	No Hit
AAAGTTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	1420	0.16337293784810228	No Hit
AAAGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1384	0.15923108871955885	No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAAC	1370	0.15762036961401418	No Hit
AAAGTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT	1299	0.14945172272160906	No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1156	0.13299937757211708	No Hit
AAAGTTGGGGACTGTGCCTGAGATGGAGCCTCTCCAGGATAGCCACAGCA	1132	0.1302381448197548	No Hit
AAAGTTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG	1110	0.127707014796756	No Hit
AAAGTTGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACAC	1108	0.12747691206739248	No Hit
AAAGTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1106	0.12724680933802898	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1101	0.12667155251462014	No Hit
AAAGTTGGGAGCCACGCTCTCGTCAGCACTAGCATCTCTTGTGAGTGCTC	1101	0.12667155251462014	No Hit
AAAGTTGGACCGACAGGATTGGTCTCTCCAGAGGCGTCTCTCCTTAGCTT	1058	0.12172434383330437	No Hit
AAAGTTGGGGGTCCGCAAAGGTAATGAGCAGCGCGCTTTCACCCGCTGCT	1040	0.11965341926903265	No Hit
AAAGTTGGGTTGTTTTTGTATTTTTCGAGTCCTGTGTAAGTTTTGAAAGC	1032	0.11873300835157856	No Hit
AAAGTTGGGCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGA	973	0.11194497783535459	No Hit
AAAGTTGGGCAATCAAGTCTGTGATGTATTATTAATAATGCCTTATATAT	964	0.11090951555321875	No Hit
AAAGTTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	918	0.10561715277785767	No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGTGCAGTGCTAAAGTTGGGATAG	904	0.104006433672313	No Hit
AAAGTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	877	0.10090004682590542	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	877	0.10090004682590542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.451540940452865E-4	0.0	0.0	0.0	0.0
8	0.0010354622821358596	0.0	0.0	0.0	0.0
9	0.0020709245642717192	0.0	0.0	0.0	0.0
10	0.02324037566571596	0.0	0.0	0.0	0.0
11	0.04360446721438786	0.0	0.0	0.0	0.0
12	0.05579991187065465	0.0	0.0	0.0	0.0
13	0.06431371285710505	0.0	0.0	0.0	0.0
14	0.08053595527723352	0.0	0.0	0.0	0.0
15	0.09503242722713555	0.0	0.0	0.0	0.0
16	0.11298044011749045	0.0	0.0	0.0	0.0
17	0.125290936138439	0.0	0.0	0.0	0.0
18	0.13691112397129698	0.0	0.0	0.0	0.0
19	0.15347852048547073	0.0	0.0	0.0	0.0
20	0.16383314330682933	0.0	0.0	0.0	0.0
21	0.1733824065754156	0.0	0.0	0.0	0.0
22	0.18810898125468115	0.0	0.0	0.0	0.0
23	0.2004194772756297	0.0	0.0	0.0	0.0
24	0.21537615468425877	0.0	0.0	0.0	0.0
25	0.2265361370583897	0.0	0.0	0.0	0.0
26	0.23884663307933826	0.0	0.0	0.0	0.0
27	0.24954640999474215	0.0	0.0	0.0	0.0
28	0.26991050154341406	0.0	0.0	0.0	0.0
29	0.28348656257586197	0.0	0.0	0.0	0.0
30	0.29786798316108226	0.0	0.0	0.0	0.0
31	0.3102935305467126	0.0	0.0	0.0	0.0
32	0.3235244374851152	0.0	0.0	0.0	0.0
33	0.3358349335060638	0.0	0.0	0.0	0.0
34	0.35079161091469285	0.0	0.0	0.0	0.0
35	0.3839264039430404	0.0	0.0	0.0	0.0
36	0.40152926273934997	0.0	0.0	0.0	0.0
37	0.4191321215356596	0.0	0.0	0.0	0.0
38	0.43247807983874403	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAC	30	2.527002E-6	44.000004	30
AGTCATA	30	2.527002E-6	44.000004	10
CCTAGAC	30	2.527002E-6	44.000004	42
CTTCACG	30	2.527002E-6	44.000004	35
CGCATAA	30	2.527002E-6	44.000004	43
CGTCTAG	30	2.527002E-6	44.000004	44
CACGACC	30	2.527002E-6	44.000004	40
TAATTGG	30	2.527002E-6	44.000004	38
GTGTCGA	30	2.527002E-6	44.000004	21
ACTACTA	30	2.527002E-6	44.000004	35
ACTACGC	30	2.527002E-6	44.000004	12
GCGATAC	30	2.527002E-6	44.000004	26
CACAACG	30	2.527002E-6	44.000004	22
GGTTAAC	30	2.527002E-6	44.000004	9
TATCGAA	30	2.527002E-6	44.000004	10
CGATACA	30	2.527002E-6	44.000004	27
AATAAGA	65	0.0	44.000004	11
TAGTAGG	30	2.527002E-6	44.000004	38
TACAAAC	30	2.527002E-6	44.000004	33
TCACGAC	25	4.4419412E-5	44.0	42
>>END_MODULE