FastQCFastQC Report
Fri 17 Jun 2016
SRR1527233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931101
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA84070.9029095662017332No Hit
AAAGTTGGGGCAGAGTGCAGTGCTAAAGTTGGGGTGTAATTTATAACAAGGG55200.5928465332976766No Hit
AAAGTTGGGCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAAT43400.466114846831869No Hit
AAAGTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG33980.3649442971278089No Hit
AAAGTTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG32930.3536673250270379No Hit
AAAGTTGGGGAAGCAGTCCTATCCAGAGTGCACTGCTAAAGTTGGGACTAAG26250.2819243025192756No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG26240.2818169027849825No Hit
AAAGTTGGGAAGAACAATAGAATATAAAGAAGTGTGAAAACCCACCCTATCC20120.2160882653976314No Hit
AAAGTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC19080.20491869303115345No Hit
AAAGTTGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAACAT18340.19697111269346718No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT18220.1956823158819505No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC17340.18623113926416146No Hit
AAAGTTGGGATTCTGAGTGCAGCTTCTCACTTAAGAAGGTTACATGGATTGA17050.18311654696966279No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAG16510.1773169613178377No Hit
AAAGTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC16120.17312837168040845No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT15810.1697989799173237No Hit
AAAGTTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT13740.1475672349186608No Hit
AAAGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC13580.14584883916997188No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAACAA13380.14370084448411075No Hit
AAAGTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG12990.1395122548466815No Hit
AAAGTTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC12190.13092027610323692No Hit
AAAGTTGGGAGCCACGCTCTCGTCAGCACTAGCATCTCTTGTGAGTGCTCCC11970.12855748194878966No Hit
AAAGTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT11860.12737608487156604No Hit
AAAGTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC11670.12533548991999793No Hit
AAAGTTGGGGACTGTGCCTGAGATGGAGCCTCTCCAGGATAGCCACAGCAAG11250.12082470107968953No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT10860.1166361114422603No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10640.11427331728781304No Hit
AAAGTTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC10560.11341411941346857No Hit
AAAGTTGGGGGGGTTAGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA10330.11094392552472826No Hit
AAAGTTGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTA10150.10901073030745322No Hit
AAAGTTGGGGGTCCGCAAAGGTAATGAGCAGCGCGCTTTCACCCGCTGCTTC9980.10718493482447125No Hit
AAAGTTGGGTTGTTTTTGTATTTTTCGAGTCCTGTGTAAGTTTTGAAAGCTC9560.10267414598416284No Hit
AAAGTTGGACCGACAGGATTGGTCTCTCCAGAGGCGTCTCTCCTTAGCTTAT9370.10063355103259475No Hit
AAAGTTGGGCAATCAAGTCTGTGATGTATTATTAATAATGCCTTATATATTG9360.10052615129830168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCA1450.046.00000419
CCACAAT253.416652E-546.024
CTATGTC301.861019E-646.025
TTCTAAC253.416652E-546.019
CTAGACG206.310833E-446.043
AACCGGT206.310833E-446.015
CAGCGAA253.416652E-546.013
ACTATAC253.416652E-546.046
AATCCGT253.416652E-546.024
GTACCGA206.310833E-446.08
ACCGGTT206.310833E-446.011
GTCGACA206.310833E-446.022
CTATCAG206.310833E-446.038
TCACACG253.416652E-546.015
TTGCTCG301.861019E-646.05
CTATATC405.6097633E-946.026
CGTTGCG206.310833E-446.045
GGCGTAA206.310833E-446.014
TAGCGGA253.416652E-546.010
GCTATGT253.416652E-546.026
Produced by FastQC (version 0.11.5)