##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527233_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	931101
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976811323368786	31.0	31.0	34.0	31.0	34.0
2	32.70588904963049	34.0	31.0	34.0	31.0	34.0
3	32.99442595379019	34.0	31.0	34.0	31.0	34.0
4	36.36991583082823	37.0	37.0	37.0	35.0	37.0
5	34.92151334817598	35.0	35.0	37.0	33.0	37.0
6	35.74080685124385	36.0	35.0	37.0	35.0	37.0
7	36.224209833304876	37.0	35.0	37.0	35.0	37.0
8	36.60077907767256	37.0	37.0	37.0	35.0	37.0
9	38.6993548497961	39.0	39.0	39.0	39.0	39.0
10	38.140394006665225	39.0	39.0	39.0	37.0	39.0
11	37.85367538000711	39.0	38.0	39.0	35.0	39.0
12	37.56024104796365	39.0	37.0	39.0	35.0	39.0
13	37.40366727132717	39.0	37.0	39.0	35.0	39.0
14	38.126246239666806	40.0	38.0	40.0	34.0	40.0
15	38.078997874559256	40.0	38.0	40.0	34.0	40.0
16	33.05855326113923	40.0	34.0	40.0	3.0	40.0
17	34.852024646091024	40.0	35.0	40.0	17.0	40.0
18	36.93283435416781	40.0	35.0	40.0	32.0	40.0
19	37.75708005898393	40.0	37.0	40.0	34.0	40.0
20	38.01774673209459	40.0	38.0	40.0	34.0	40.0
21	38.20894618306714	40.0	38.0	40.0	34.0	40.0
22	38.29360187562896	40.0	38.0	40.0	35.0	40.0
23	38.254559924218746	40.0	38.0	40.0	35.0	40.0
24	38.171632293381705	40.0	38.0	40.0	34.0	40.0
25	38.13389202675113	40.0	38.0	40.0	34.0	40.0
26	38.015661029254616	40.0	38.0	40.0	34.0	40.0
27	37.90459681602748	40.0	38.0	40.0	34.0	40.0
28	37.789906787770605	40.0	37.0	40.0	34.0	40.0
29	37.559165976623376	40.0	37.0	40.0	33.0	40.0
30	37.58913157648848	40.0	37.0	40.0	33.0	40.0
31	37.48130653924762	40.0	37.0	40.0	33.0	40.0
32	37.41713627200486	40.0	36.0	40.0	33.0	40.0
33	37.103027491109984	39.0	36.0	40.0	33.0	40.0
34	37.09628278779638	39.0	36.0	40.0	32.0	40.0
35	36.9024810412619	39.0	35.0	40.0	31.0	40.0
36	36.87793375799188	39.0	35.0	40.0	32.0	40.0
37	36.444299812802264	39.0	35.0	40.0	31.0	40.0
38	36.45466603515623	39.0	35.0	40.0	31.0	40.0
39	36.0521930488744	38.0	35.0	40.0	30.0	40.0
40	35.927613653083824	38.0	35.0	40.0	29.0	40.0
41	35.86263466584184	38.0	35.0	40.0	30.0	40.0
42	35.91329512050787	38.0	35.0	40.0	30.0	40.0
43	35.82575467108294	38.0	35.0	40.0	30.0	40.0
44	36.1061098634842	38.0	35.0	40.0	30.0	40.0
45	36.0959681065749	38.0	35.0	40.0	31.0	40.0
46	36.03020295327789	38.0	35.0	40.0	31.0	40.0
47	35.71029136473916	38.0	35.0	40.0	30.0	40.0
48	35.645787084322755	37.0	35.0	40.0	30.0	40.0
49	35.563129026818785	37.0	35.0	40.0	30.0	40.0
50	34.99940070948264	37.0	34.0	40.0	28.0	40.0
51	35.05404891628298	36.0	34.0	40.0	28.0	40.0
52	34.76823459538761	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	2.0
15	9.0
16	17.0
17	36.0
18	88.0
19	165.0
20	333.0
21	565.0
22	950.0
23	1597.0
24	2362.0
25	3633.0
26	4960.0
27	6766.0
28	9024.0
29	11444.0
30	14842.0
31	20037.0
32	26839.0
33	38071.0
34	53280.0
35	71206.0
36	114897.0
37	188607.0
38	267306.0
39	94063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.65610605079364	0.267747537592592	0.06583603712164415	0.010310374492133506
8	99.39480249725861	0.4562340712769076	0.08087199992267219	0.06809143154179836
9	97.44313452568518	1.559444141935193	0.43733171804132953	0.5600896143382941
10	51.75732815237015	32.7011784972844	5.598533349228494	9.942960001116957
11	31.0783685121163	26.029829202202553	23.758217422170098	19.133584863511047
12	32.13260430393695	17.842640057308497	26.73512325730506	23.289632381449486
13	25.20242164920884	17.239053550581517	29.364913151204863	28.19361164900478
14	25.609037043242356	19.56372079935474	29.86851050530501	24.958731652097892
15	25.519787864044822	20.598409839534057	28.347085869309556	25.534716427111558
16	28.90073149959027	28.803212540852176	22.80869637128518	19.48735958827238
17	31.74725405729346	22.569946761951712	21.633528478650543	24.049270702104284
18	30.55146541567456	20.272022047017455	23.80783609941349	25.368676437894493
19	28.4780061454128	21.374158120332808	23.633741130124445	26.51409460412995
20	26.638033897504137	22.593682103230478	24.095559987584593	26.672724011680792
21	27.826304557722526	21.59959016261394	28.166439516228635	22.4076657634349
22	28.834895462468623	21.353322571879957	25.053350818010077	24.758431147641343
23	26.680134593347017	23.40916828571766	25.724706557075976	24.185990563859345
24	26.456957945486042	24.7250298302762	25.178793707664365	23.63921851657339
25	29.75004859837977	21.93757712643419	23.33678086480414	24.9755934103819
26	28.933273619081067	21.898805822354394	22.903745136134535	26.264175422430004
27	27.845206910958105	21.383072298279135	24.466626069567106	26.30509472119566
28	32.40271463568399	21.361592351420523	20.46963755811668	25.7660554547788
29	29.403577055550368	22.47543499577382	23.687870596208143	24.43311735246767
30	31.495401680376244	19.81686197308348	23.578215467494935	25.109520879045345
31	29.256976418240342	21.93789932563707	23.455027972260797	25.350096283861795
32	31.775392787678243	21.16838022942731	22.801822788290423	24.254404194604025
33	31.76411581557747	21.15667365838937	22.514421099322202	24.56478942671096
34	28.004158517711826	24.12896130494973	24.752846361458104	23.11403381588034
35	28.40669272184221	23.861858165762897	25.191359476576654	22.54008963581824
36	29.52386475795859	23.002875090887027	22.19898807970349	25.27427207145089
37	26.724598083344343	25.818359125379526	22.80429298217916	24.65274980909697
38	30.362227083850197	24.362663126771427	21.607537742951624	23.66757204642676
39	28.46007038978586	23.80740650047632	21.540842507955634	26.191680601782192
40	28.279638836173522	23.4611497571155	23.676271424904492	24.582939981806483
41	26.67949019494126	24.094593389975955	23.09190947061597	26.13400694446682
42	24.941977293548177	22.6489929663914	24.849613522056146	27.55941621800428
43	27.205963692445824	24.014043589256158	24.9369295060364	23.84306321226161
44	29.26470919910944	22.302521423562	23.899233273296883	24.53353610403168
45	26.02961440273397	22.434515697008166	23.827275451320535	27.708594448937333
46	27.466622847575074	22.972695765550675	25.138841006507352	24.4218403803669
47	27.006092786926445	25.007061532529768	23.437844014773905	24.54900166576988
48	27.034446316779814	25.310143582704775	22.05743522990524	25.59797487061017
49	27.04733428489498	26.234855294968	21.50658199271615	25.211228427420874
50	25.254510520340972	27.062692446898883	21.67455517715049	26.008241855609647
51	25.053350818010077	28.111987850942054	21.97280423928231	24.861857091765554
52	27.122406699165825	24.99374396547743	22.459002836426983	25.42484649892976
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	6.0
2	12.0
3	7.0
4	2.0
5	63.5
6	125.0
7	769.0
8	1413.0
9	1394.5
10	1376.0
11	2026.5
12	2677.0
13	2341.5
14	2210.5
15	2415.0
16	2356.5
17	2298.0
18	2184.5
19	2071.0
20	2585.0
21	3099.0
22	3144.5
23	3190.0
24	4143.5
25	5097.0
26	6591.0
27	8085.0
28	9014.0
29	9943.0
30	9211.0
31	8479.0
32	10253.0
33	12027.0
34	14088.5
35	16150.0
36	18818.5
37	21487.0
38	20083.0
39	22703.0
40	26727.0
41	29570.5
42	32414.0
43	37780.0
44	43146.0
45	42965.5
46	42785.0
47	47416.5
48	52048.0
49	55811.0
50	59574.0
51	65109.0
52	70644.0
53	77978.5
54	85313.0
55	82082.5
56	78852.0
57	74119.5
58	69387.0
59	65690.5
60	61994.0
61	56085.0
62	50176.0
63	45689.0
64	35116.5
65	29031.0
66	27608.0
67	26185.0
68	22323.0
69	18461.0
70	15938.0
71	13415.0
72	9569.0
73	5723.0
74	4071.5
75	2420.0
76	1458.5
77	497.0
78	343.5
79	190.0
80	218.5
81	247.0
82	139.5
83	32.0
84	17.5
85	3.0
86	2.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	931101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.269692855569884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39603127173056	9.47403406082527
2	10.094257279973421	2.678953874206952
3	4.141535028477542	1.6487069333544258
4	2.2808481426468306	1.2106461721248194
5	1.4595528680902858	0.9683909133012099
6	1.0316383048922195	0.8213714066376306
7	0.7632166789541193	0.7089355638378474
8	0.6079428893144325	0.645377233194419
9	0.4713937976865662	0.5629725818389244
>10	4.268535010257948	12.499666016791265
>50	1.6775047981675844	16.262722452883473
>100	1.7213930050963875	40.370467811127945
>500	0.06176858752942655	5.398259255508444
>1k	0.022756848037157152	5.247486761018476
>5k	0.001625489145511225	1.502008963348905
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8407	0.9029095662017332	No Hit
AAAGTTGGGGCAGAGTGCAGTGCTAAAGTTGGGGTGTAATTTATAACAAGGG	5520	0.5928465332976766	No Hit
AAAGTTGGGCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAAT	4340	0.466114846831869	No Hit
AAAGTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	3398	0.3649442971278089	No Hit
AAAGTTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	3293	0.3536673250270379	No Hit
AAAGTTGGGGAAGCAGTCCTATCCAGAGTGCACTGCTAAAGTTGGGACTAAG	2625	0.2819243025192756	No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2624	0.2818169027849825	No Hit
AAAGTTGGGAAGAACAATAGAATATAAAGAAGTGTGAAAACCCACCCTATCC	2012	0.2160882653976314	No Hit
AAAGTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1908	0.20491869303115345	No Hit
AAAGTTGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAACAT	1834	0.19697111269346718	No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1822	0.1956823158819505	No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1734	0.18623113926416146	No Hit
AAAGTTGGGATTCTGAGTGCAGCTTCTCACTTAAGAAGGTTACATGGATTGA	1705	0.18311654696966279	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAG	1651	0.1773169613178377	No Hit
AAAGTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1612	0.17312837168040845	No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1581	0.1697989799173237	No Hit
AAAGTTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1374	0.1475672349186608	No Hit
AAAGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1358	0.14584883916997188	No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAACAA	1338	0.14370084448411075	No Hit
AAAGTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1299	0.1395122548466815	No Hit
AAAGTTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	1219	0.13092027610323692	No Hit
AAAGTTGGGAGCCACGCTCTCGTCAGCACTAGCATCTCTTGTGAGTGCTCCC	1197	0.12855748194878966	No Hit
AAAGTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	1186	0.12737608487156604	No Hit
AAAGTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC	1167	0.12533548991999793	No Hit
AAAGTTGGGGACTGTGCCTGAGATGGAGCCTCTCCAGGATAGCCACAGCAAG	1125	0.12082470107968953	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1086	0.1166361114422603	No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1064	0.11427331728781304	No Hit
AAAGTTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	1056	0.11341411941346857	No Hit
AAAGTTGGGGGGGTTAGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1033	0.11094392552472826	No Hit
AAAGTTGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTA	1015	0.10901073030745322	No Hit
AAAGTTGGGGGTCCGCAAAGGTAATGAGCAGCGCGCTTTCACCCGCTGCTTC	998	0.10718493482447125	No Hit
AAAGTTGGGTTGTTTTTGTATTTTTCGAGTCCTGTGTAAGTTTTGAAAGCTC	956	0.10267414598416284	No Hit
AAAGTTGGACCGACAGGATTGGTCTCTCCAGAGGCGTCTCTCCTTAGCTTAT	937	0.10063355103259475	No Hit
AAAGTTGGGCAATCAAGTCTGTGATGTATTATTAATAATGCCTTATATATTG	936	0.10052615129830168	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.147994685861147E-4	0.0	0.0	0.0	0.0
8	7.517981400514015E-4	0.0	0.0	0.0	0.0
9	0.0020405949515680897	0.0	0.0	0.0	0.0
10	0.022983543138714275	0.0	0.0	0.0	0.0
11	0.041993296108585425	0.0	0.0	0.0	0.0
12	0.0526258698035981	0.0	0.0	0.0	0.0
13	0.059929051735526005	0.0	0.0	0.0	0.0
14	0.07496501453655403	0.0	0.0	0.0	0.0
15	0.08645678610591118	0.0	0.0	0.0	0.0
16	0.10396294279567952	0.0	0.0	0.0	0.0
17	0.1153473146307436	0.0	0.0	0.0	0.0
18	0.12576508885717017	0.0	0.0	0.0	0.0
19	0.14294904634405933	0.0	0.0	0.0	0.0
20	0.15325942083619284	0.0	0.0	0.0	0.0
21	0.1651807913427222	0.0	0.0	0.0	0.0
22	0.17763916052071688	0.0	0.0	0.0	0.0
23	0.18848653368431567	0.0	0.0	0.0	0.0
24	0.20191150047094783	0.0	0.0	0.0	0.0
25	0.2138328709774772	0.0	0.0	0.0	0.0
26	0.22575424148400658	0.0	0.0	0.0	0.0
27	0.23713861331907066	0.0	0.0	0.0	0.0
28	0.25625576602323485	0.0	0.0	0.0	0.0
29	0.26731793865541975	0.0	0.0	0.0	0.0
30	0.2813873038478103	0.0	0.0	0.0	0.0
31	0.2943826716972702	0.0	0.0	0.0	0.0
32	0.3086668363582469	0.0	0.0	0.0	0.0
33	0.3191920103189665	0.0	0.0	0.0	0.0
34	0.3360537686029765	0.0	0.0	0.0	0.0
35	0.36988468490528953	0.0	0.0	0.0	0.0
36	0.3873908415950579	0.0	0.0	0.0	0.0
37	0.4001714099759317	0.0	0.0	0.0	0.0
38	0.41381117623115	0.0	0.0	0.0	0.0
39	0.4289545387664711	0.0	0.0	0.0	0.0
40	0.4448496994418436	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCA	145	0.0	46.000004	19
CCACAAT	25	3.416652E-5	46.0	24
CTATGTC	30	1.861019E-6	46.0	25
TTCTAAC	25	3.416652E-5	46.0	19
CTAGACG	20	6.310833E-4	46.0	43
AACCGGT	20	6.310833E-4	46.0	15
CAGCGAA	25	3.416652E-5	46.0	13
ACTATAC	25	3.416652E-5	46.0	46
AATCCGT	25	3.416652E-5	46.0	24
GTACCGA	20	6.310833E-4	46.0	8
ACCGGTT	20	6.310833E-4	46.0	11
GTCGACA	20	6.310833E-4	46.0	22
CTATCAG	20	6.310833E-4	46.0	38
TCACACG	25	3.416652E-5	46.0	15
TTGCTCG	30	1.861019E-6	46.0	5
CTATATC	40	5.6097633E-9	46.0	26
CGTTGCG	20	6.310833E-4	46.0	45
GGCGTAA	20	6.310833E-4	46.0	14
TAGCGGA	25	3.416652E-5	46.0	10
GCTATGT	25	3.416652E-5	46.0	26
>>END_MODULE