FastQCFastQC Report
Fri 17 Jun 2016
SRR1527232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902898
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA83160.9210342696517215No Hit
AAAGTTGGGGCAGAGTGCAGTGCTAAAGTTGGGGTGTAATTTATAACAAGGG52810.5848944177526144No Hit
AAAGTTGGGCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAAT38230.42341438346302684No Hit
AAAGTTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG34960.38719766795363375No Hit
AAAGTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG31790.3520884972610417No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG25100.277993749017054No Hit
AAAGTTGGGGAAGCAGTCCTATCCAGAGTGCACTGCTAAAGTTGGGACTAAG24720.2737850787132101No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT18960.20999049726547186No Hit
AAAGTTGGGAAGAACAATAGAATATAAAGAAGTGTGAAAACCCACCCTATCC18100.20046561184098316No Hit
AAAGTTGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAACAT17790.1970322229089No Hit
AAAGTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC17730.19636769601881937No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC16400.18163734995536596No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAG16340.18097282306528534No Hit
AAAGTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC15860.17565660794464047No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT14750.16336286047814924No Hit
AAAGTTGGGATTCTGAGTGCAGCTTCTCACTTAAGAAGGTTACATGGATTGA14110.15627457365062278No Hit
AAAGTTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT13930.15428099298038095No Hit
AAAGTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG13400.1484110054513356No Hit
AAAGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC13080.14486686203757235No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAACAA12680.1404366827703683No Hit
AAAGTTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC12400.13733555728332547No Hit
AAAGTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC11540.12781067185883677No Hit
AAAGTTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC11010.12194068432979141No Hit
AAAGTTGGGAGCCACGCTCTCGTCAGCACTAGCATCTCTTGTGAGTGCTCCC10800.11961484021450929No Hit
AAAGTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT10720.11872880436106846No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT10640.11784276850762766No Hit
AAAGTTGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTA10220.11319108027706341No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10120.1120835354602624No Hit
AAAGTTGGGCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAGC9780.10831788308313896No Hit
AAAGTTGGACCGACAGGATTGGTCTCTCCAGAGGCGTCTCTCCTTAGCTTAT9720.10765335619305837No Hit
AAAGTTGGGTTGTTTTTGTATTTTTCGAGTCCTGTGTAAGTTTTGAAAGCTC9720.10765335619305837No Hit
AAAGTTGGGGGTCCGCAAAGGTAATGAGCAGCGCGCTTTCACCCGCTGCTTC9660.10698882930297775No Hit
AAAGTTGGGGACTGTGCCTGAGATGGAGCCTCTCCAGGATAGCCACAGCAAG9570.10599203896785683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTA405.6097633E-946.00000429
AACGTTA206.31073E-446.00000425
CTATAGG800.046.00000443
CGTCTAG206.31073E-446.00000444
TAACGGC206.31073E-446.00000425
TTATAGG206.31073E-446.00000446
GTTACGG206.31073E-446.00000422
TAACCGA206.31073E-446.00000444
ACTCGTC206.31073E-446.00000435
ACTAAGC206.31073E-446.00000424
CCCTTAT206.31073E-446.00000417
TTTATCG206.31073E-446.00000446
TATAGGT206.31073E-446.00000417
TTACGGT206.31073E-446.00000423
TTCATAC206.31073E-446.00000428
CAATCTA206.31073E-446.00000442
TCGGTTA206.31073E-446.00000441
TGCGTAT206.31073E-446.00000439
TAGTAAT206.31073E-446.00000423
TCACGAC253.4165692E-546.042
Produced by FastQC (version 0.11.5)