##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	902898
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.911047538038627	31.0	31.0	34.0	30.0	34.0
2	32.679479852652236	34.0	31.0	34.0	31.0	34.0
3	32.9625372965717	34.0	31.0	34.0	31.0	34.0
4	36.36327691500036	37.0	37.0	37.0	35.0	37.0
5	34.98925017000813	35.0	35.0	37.0	33.0	37.0
6	35.7591300456973	36.0	35.0	37.0	35.0	37.0
7	36.24197860666432	37.0	35.0	37.0	35.0	37.0
8	36.62649823125093	37.0	37.0	37.0	35.0	37.0
9	38.64093064775866	39.0	39.0	39.0	39.0	39.0
10	38.134182377189894	39.0	39.0	39.0	37.0	39.0
11	37.78876240727081	39.0	38.0	39.0	35.0	39.0
12	37.52847719232959	39.0	37.0	39.0	35.0	39.0
13	37.306735644557854	39.0	37.0	39.0	35.0	39.0
14	38.05091937295243	40.0	38.0	40.0	34.0	40.0
15	38.185169310376146	40.0	38.0	40.0	34.0	40.0
16	38.27906252976527	40.0	38.0	40.0	34.0	40.0
17	38.33996863433079	40.0	38.0	40.0	35.0	40.0
18	38.336624956528865	40.0	38.0	40.0	35.0	40.0
19	38.27740675026415	40.0	38.0	40.0	35.0	40.0
20	38.2484510985737	40.0	38.0	40.0	34.0	40.0
21	33.97120383476317	40.0	35.0	40.0	10.0	40.0
22	34.81160108893806	40.0	35.0	40.0	17.0	40.0
23	36.88108180547526	40.0	35.0	40.0	30.0	40.0
24	37.584492378984116	40.0	37.0	40.0	33.0	40.0
25	37.854663538960104	40.0	37.0	40.0	34.0	40.0
26	37.905376908576606	40.0	38.0	40.0	34.0	40.0
27	37.867334959209124	40.0	38.0	40.0	34.0	40.0
28	37.765740980708784	40.0	37.0	40.0	34.0	40.0
29	37.68827043586319	40.0	37.0	40.0	34.0	40.0
30	37.63789929759508	40.0	37.0	40.0	34.0	40.0
31	37.44984151033672	40.0	37.0	40.0	33.0	40.0
32	37.420182567687604	40.0	36.0	40.0	33.0	40.0
33	37.1100556209007	40.0	36.0	40.0	33.0	40.0
34	37.09186530482956	39.0	36.0	40.0	32.0	40.0
35	36.903490759753595	39.0	35.0	40.0	31.0	40.0
36	36.81531800934325	39.0	35.0	40.0	31.0	40.0
37	36.60394086596714	39.0	35.0	40.0	31.0	40.0
38	36.54128041041181	39.0	35.0	40.0	31.0	40.0
39	36.3602466723816	39.0	35.0	40.0	31.0	40.0
40	31.380247824228206	37.0	30.0	40.0	8.0	40.0
41	32.28990650106657	37.0	30.0	40.0	13.0	40.0
42	34.180639452075425	37.0	31.0	40.0	24.0	40.0
43	35.070306944970525	37.0	33.0	40.0	27.0	40.0
44	35.8047808279562	38.0	35.0	40.0	30.0	40.0
45	35.97174763926822	38.0	35.0	40.0	30.0	40.0
46	36.006731657396514	38.0	35.0	40.0	31.0	40.0
47	35.92212630884109	38.0	35.0	40.0	31.0	40.0
48	35.758559660116646	37.0	35.0	40.0	30.0	40.0
49	35.62314569309047	37.0	35.0	40.0	30.0	40.0
50	35.45095237778797	37.0	35.0	40.0	30.0	40.0
51	35.25522373512844	37.0	34.0	40.0	29.0	40.0
52	34.90479766263742	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	2.0
15	12.0
16	23.0
17	58.0
18	95.0
19	197.0
20	362.0
21	650.0
22	1111.0
23	1726.0
24	2499.0
25	3574.0
26	5053.0
27	6980.0
28	9306.0
29	12062.0
30	16375.0
31	21390.0
32	29654.0
33	40014.0
34	61787.0
35	85745.0
36	118462.0
37	129825.0
38	258987.0
39	96946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.65068036478097	0.26481396569712196	0.07187965861038567	0.012626010911531536
8	99.41831746221611	0.4513245128464123	0.0643483538561388	0.0660096710813403
9	97.36038843811815	1.5917634107064143	0.4777948339679565	0.5700533172074809
10	51.665415141023686	32.66636984465576	5.648700074648521	10.019514939672034
11	31.06607833885998	26.03893241540019	23.819634111494324	19.07535513424551
12	32.25524920865922	17.810538953458753	26.701244215847193	23.232967622034824
13	25.31282603350545	17.14867017093847	29.391359821375175	28.147143974180917
14	25.49269131175393	19.674758389098216	29.95720446827881	24.875345830869048
15	25.454370261092613	20.431986780345067	28.483173071598344	25.630469886963976
16	30.369432649092143	21.05841412872772	25.952876183134748	22.619277039045386
17	31.74766141911933	22.639988127119565	21.572314923723386	24.040035530037724
18	30.555610932796395	20.39067535867839	23.83912690027002	25.214586808255195
19	28.559039891549208	21.318687160675957	23.78507871321013	26.337194234564702
20	26.578085232218925	22.681964075676323	24.075587718657037	26.66436297344772
21	32.67545171215353	20.69569320122539	26.404975977352922	20.223879109268157
22	28.88587636698719	21.3412810749387	25.05487884567249	24.717963712401623
23	26.694598946946392	23.33907041548436	25.781428245493952	24.184902392075294
24	26.582293902522764	24.696809606400723	25.135951126262324	23.584945364814185
25	29.701140106634416	21.961727681310624	23.39278633909921	24.94434587295575
26	28.84445419083883	21.908454775622495	22.89948587769604	26.34760515584263
27	27.80147923685732	21.480167194965542	24.39699722449269	26.32135634368445
28	32.42171319462442	21.326772237838604	20.550494075742776	25.701020491794203
29	29.296553985057006	22.54750813491668	23.698911726463013	24.457026153563305
30	31.534348287403454	19.84532029088557	23.59912193846924	25.02120948324174
31	29.432449734078492	22.027294334465246	23.301746155158167	25.238509776298095
32	31.801931115142573	21.23285243737388	22.770789169983765	24.194427277499784
33	31.92021690157692	21.175481615863585	22.456800214420678	24.447501268138815
34	27.998732968729577	24.197417648505144	24.75473420031942	23.04911518244586
35	28.389585534578654	23.967048326610534	25.160427866713626	22.48293827209718
36	29.678324683408313	22.92108300162366	22.1973024638442	25.203289851123827
37	26.55349773728594	25.920535874484163	22.900261159067803	24.625705229162097
38	30.521166288993882	24.381602351539154	21.578295665734114	23.51893569373285
39	28.180702582129985	23.870913436512208	21.572314923723386	26.376069057634417
40	28.62272371851527	27.85652421425233	21.66280133525603	21.85795073197637
41	26.745989026445955	24.239615106025266	22.939911263509277	26.074484604019503
42	24.896832200314986	22.674543525403756	24.81454162042667	27.614082653854588
43	27.321247804292405	24.16729242948816	24.775666797356955	23.735792968862484
44	29.271634226678984	22.353577037494823	23.923854078755298	24.4509346570709
45	26.00747814260304	22.49345994785679	23.820630901829443	27.678431007710724
46	27.45603600849708	23.131405762334172	25.055432618080893	24.357125611087852
47	26.790290819117995	25.011130825408852	23.475962954840966	24.722615400632186
48	26.98876285028874	25.44949706389869	21.999827223008577	25.561912862803993
49	27.17372283469451	26.280709448907853	21.384586077275618	25.160981639122028
50	25.114907774743106	27.24261212229953	21.708875199634953	25.93360490332241
51	25.00647913717828	28.17682617527118	22.014779078035392	24.801915609515138
52	27.123883317938464	25.076254460636747	22.371408509045317	25.428453712379472
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	3.5
4	3.0
5	58.5
6	114.0
7	705.5
8	1297.0
9	1293.5
10	1290.0
11	1937.5
12	2585.0
13	2225.0
14	2144.0
15	2423.0
16	2350.0
17	2277.0
18	2179.0
19	2081.0
20	2546.0
21	3011.0
22	3046.0
23	3081.0
24	3933.0
25	4785.0
26	6138.0
27	7491.0
28	8491.5
29	9492.0
30	8866.0
31	8240.0
32	10064.5
33	11889.0
34	13813.5
35	15738.0
36	18067.0
37	20396.0
38	19141.5
39	21832.5
40	25778.0
41	28459.0
42	31140.0
43	36336.0
44	41532.0
45	41449.0
46	41366.0
47	46155.0
48	50944.0
49	54187.0
50	57430.0
51	62089.0
52	66748.0
53	74988.5
54	83229.0
55	80284.5
56	77340.0
57	72435.0
58	67530.0
59	63954.5
60	60379.0
61	54382.5
62	48386.0
63	44814.0
64	34886.5
65	28531.0
66	27124.5
67	25718.0
68	21761.5
69	17805.0
70	15371.5
71	12938.0
72	9176.0
73	5414.0
74	3924.5
75	2435.0
76	1472.0
77	509.0
78	375.0
79	241.0
80	264.5
81	288.0
82	155.0
83	22.0
84	12.0
85	2.0
86	1.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	902898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.779146424259824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.22535333292741	9.2630798699701
2	11.57867833674542	3.190886084028407
3	5.5294187587937	2.2857201215540224
4	3.2811146800351194	1.8084383844396936
5	2.0165793265271796	1.3893370908176637
6	1.374784080980197	1.136601069214057
7	0.9059439236322967	0.8738193783128514
8	0.6868082054832509	0.7570904662989475
9	0.5079196516545773	0.6298829326716718
>10	3.540652540780494	11.041276144253061
>50	1.642731233285752	16.55869071030076
>100	1.6337052374984677	39.62139416938305
>500	0.05333542965213481	4.8708017247719875
>1k	0.021334171860853922	5.035649192615941
>5k	0.0016410901431426093	1.5373326613677973
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8316	0.9210342696517215	No Hit
AAAGTTGGGGCAGAGTGCAGTGCTAAAGTTGGGGTGTAATTTATAACAAGGG	5281	0.5848944177526144	No Hit
AAAGTTGGGCATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAAT	3823	0.42341438346302684	No Hit
AAAGTTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	3496	0.38719766795363375	No Hit
AAAGTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	3179	0.3520884972610417	No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2510	0.277993749017054	No Hit
AAAGTTGGGGAAGCAGTCCTATCCAGAGTGCACTGCTAAAGTTGGGACTAAG	2472	0.2737850787132101	No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1896	0.20999049726547186	No Hit
AAAGTTGGGAAGAACAATAGAATATAAAGAAGTGTGAAAACCCACCCTATCC	1810	0.20046561184098316	No Hit
AAAGTTGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAACAT	1779	0.1970322229089	No Hit
AAAGTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1773	0.19636769601881937	No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1640	0.18163734995536596	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAG	1634	0.18097282306528534	No Hit
AAAGTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1586	0.17565660794464047	No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1475	0.16336286047814924	No Hit
AAAGTTGGGATTCTGAGTGCAGCTTCTCACTTAAGAAGGTTACATGGATTGA	1411	0.15627457365062278	No Hit
AAAGTTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1393	0.15428099298038095	No Hit
AAAGTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1340	0.1484110054513356	No Hit
AAAGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1308	0.14486686203757235	No Hit
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAACAA	1268	0.1404366827703683	No Hit
AAAGTTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	1240	0.13733555728332547	No Hit
AAAGTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC	1154	0.12781067185883677	No Hit
AAAGTTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	1101	0.12194068432979141	No Hit
AAAGTTGGGAGCCACGCTCTCGTCAGCACTAGCATCTCTTGTGAGTGCTCCC	1080	0.11961484021450929	No Hit
AAAGTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	1072	0.11872880436106846	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1064	0.11784276850762766	No Hit
AAAGTTGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTA	1022	0.11319108027706341	No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1012	0.1120835354602624	No Hit
AAAGTTGGGCCGCGCCGTCATGTCTGTCCTGACGCCACTGCTGCTGAGGAGC	978	0.10831788308313896	No Hit
AAAGTTGGACCGACAGGATTGGTCTCTCCAGAGGCGTCTCTCCTTAGCTTAT	972	0.10765335619305837	No Hit
AAAGTTGGGTTGTTTTTGTATTTTTCGAGTCCTGTGTAAGTTTTGAAAGCTC	972	0.10765335619305837	No Hit
AAAGTTGGGGGTCCGCAAAGGTAATGAGCAGCGCGCTTTCACCCGCTGCTTC	966	0.10698882930297775	No Hit
AAAGTTGGGGACTGTGCCTGAGATGGAGCCTCTCCAGGATAGCCACAGCAAG	957	0.10599203896785683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.3226344504030356E-4	0.0	0.0	0.0	0.0
8	0.0013290537801612142	0.0	0.0	0.0	0.0
9	0.002547353078642327	0.0	0.0	0.0	0.0
10	0.02060033359249882	0.0	0.0	0.0	0.0
11	0.043637265781959866	0.0	0.0	0.0	0.0
12	0.0560417677301312	0.0	0.0	0.0	0.0
13	0.06346231800269798	0.0	0.0	0.0	0.0
14	0.07730662821271063	0.0	0.0	0.0	0.0
15	0.08904660327080136	0.0	0.0	0.0	0.0
16	0.10743184722969815	0.0	0.0	0.0	0.0
17	0.11662446920914654	0.0	0.0	0.0	0.0
18	0.12570633670691483	0.0	0.0	0.0	0.0
19	0.14187649103220962	0.0	0.0	0.0	0.0
20	0.15239816679181922	0.0	0.0	0.0	0.0
21	0.16291984255142886	0.0	0.0	0.0	0.0
22	0.17598887138968078	0.0	0.0	0.0	0.0
23	0.18994393608137353	0.0	0.0	0.0	0.0
24	0.204674282144827	0.0	0.0	0.0	0.0
25	0.2148636944593963	0.0	0.0	0.0	0.0
26	0.22527461573732582	0.0	0.0	0.0	0.0
27	0.23679308183205633	0.0	0.0	0.0	0.0
28	0.25971925953983727	0.0	0.0	0.0	0.0
29	0.2717914980429683	0.0	0.0	0.0	0.0
30	0.28618958066138145	0.0	0.0	0.0	0.0
31	0.2997016274263538	0.0	0.0	0.0	0.0
32	0.3112200935210843	0.0	0.0	0.0	0.0
33	0.32284931409749495	0.0	0.0	0.0	0.0
34	0.33680437878918773	0.0	0.0	0.0	0.0
35	0.36814789710465634	0.0	0.0	0.0	0.0
36	0.3844288059116312	0.0	0.0	0.0	0.0
37	0.39727632578652294	0.0	0.0	0.0	0.0
38	0.40923780980797386	0.0	0.0	0.0	0.0
39	0.4220853296828656	0.0	0.0	0.0	0.0
40	0.43980604675168183	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATTA	40	5.6097633E-9	46.000004	29
AACGTTA	20	6.31073E-4	46.000004	25
CTATAGG	80	0.0	46.000004	43
CGTCTAG	20	6.31073E-4	46.000004	44
TAACGGC	20	6.31073E-4	46.000004	25
TTATAGG	20	6.31073E-4	46.000004	46
GTTACGG	20	6.31073E-4	46.000004	22
TAACCGA	20	6.31073E-4	46.000004	44
ACTCGTC	20	6.31073E-4	46.000004	35
ACTAAGC	20	6.31073E-4	46.000004	24
CCCTTAT	20	6.31073E-4	46.000004	17
TTTATCG	20	6.31073E-4	46.000004	46
TATAGGT	20	6.31073E-4	46.000004	17
TTACGGT	20	6.31073E-4	46.000004	23
TTCATAC	20	6.31073E-4	46.000004	28
CAATCTA	20	6.31073E-4	46.000004	42
TCGGTTA	20	6.31073E-4	46.000004	41
TGCGTAT	20	6.31073E-4	46.000004	39
TAGTAAT	20	6.31073E-4	46.000004	23
TCACGAC	25	3.4165692E-5	46.0	42
>>END_MODULE