FastQCFastQC Report
Fri 17 Jun 2016
SRR1527231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972515
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC176400.5934368707979606No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT158170.532108332506312No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG128300.43162103471302926No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC123490.415439451104536No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC100900.3394431987727564No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGA93730.3153222103168529No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC89030.2995106837139594No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT88750.2985687204269785No Hit
ATGCTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA78320.26348058798694035No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG73270.2464916072753207No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG72810.244944096160995No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA72110.24258918794354276No Hit
ATGCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC70820.23824942851423794No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT70450.23700469131358462No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA67150.22590298114559554No Hit
ATGCTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA64410.2166851975515683No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC62800.21126890865142814No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC62340.2097213975371024No Hit
ATGCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC61160.20575169511339725No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT60490.2034977115338358No Hit
ATGCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC60110.20221933278721888No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC58980.19841783809333174No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG58400.19646662842744278No Hit
ATGCTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG58320.1961974960597339No Hit
ATGCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT57270.1926651337335556No Hit
ATGCTTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT55070.18526399362156287No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53530.18008319554316798No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC50360.16941882547270576No Hit
ATGCTTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA49250.16568461387074582No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37930.127602383839947No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT36680.1233971905944966No Hit
ATGCTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC36540.12292620895100613No Hit
ATGCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG35600.11976390363042744No Hit
ATGCTTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC35420.11915835580308257No Hit
ATGCTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA34720.11680344758563034No Hit
ATGCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT33480.11263189588614354No Hit
ATGCTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT33200.11168993259916267No Hit
ATGCTTGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAA32150.10815757027298432No Hit
ATGCTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG29870.1004872977932828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA351.4471152E-744.018
CGTATTA302.5290774E-644.034
CGTATAG351.4471152E-744.040
ACGTTAA302.5290774E-644.010
TACGGGT408.3200575E-944.024
ACTTACG351.4471152E-744.019
TTATCGA207.8585E-444.038
TCGATAG408.3200575E-944.017
ATATACG207.8585E-444.029
GTCAATC207.8585E-444.039
TTACGTA502.7284841E-1144.044
TATAGGG351.4471152E-744.043
ACGTATC207.8585E-444.043
ATAACGT207.8585E-444.011
ACGTAAT254.4445507E-544.021
ACGTAAC502.7284841E-1144.029
CGATCTA502.7284841E-1144.044
TATAACG454.802132E-1044.035
CGATAAG207.8585E-444.028
TTGGGAT149800.041.5327075
Produced by FastQC (version 0.11.5)