##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2972515
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45464598160144	33.0	31.0	34.0	31.0	34.0
2	32.83755910399106	34.0	31.0	34.0	31.0	34.0
3	33.032769893507684	34.0	31.0	34.0	31.0	34.0
4	36.35093750578214	37.0	37.0	37.0	35.0	37.0
5	36.30145617431703	37.0	37.0	37.0	35.0	37.0
6	36.46001786366091	37.0	37.0	37.0	35.0	37.0
7	36.52898976119548	37.0	37.0	37.0	35.0	37.0
8	36.64953145736859	37.0	37.0	37.0	35.0	37.0
9	38.754049685199234	39.0	39.0	39.0	39.0	39.0
10	38.27496480253254	39.0	39.0	39.0	37.0	39.0
11	37.95582057617876	39.0	38.0	39.0	35.0	39.0
12	37.50074600128174	39.0	37.0	39.0	35.0	39.0
13	37.332362662593795	39.0	37.0	39.0	35.0	39.0
14	38.16092870851787	40.0	38.0	40.0	35.0	40.0
15	38.17086507553368	40.0	38.0	40.0	35.0	40.0
16	38.0576817947092	40.0	38.0	40.0	34.0	40.0
17	38.114801102769874	40.0	38.0	40.0	34.0	40.0
18	38.1130090176164	40.0	38.0	40.0	34.0	40.0
19	38.02660070680888	40.0	38.0	40.0	34.0	40.0
20	37.80732578304904	40.0	38.0	40.0	33.0	40.0
21	37.99993036199986	40.0	38.0	40.0	34.0	40.0
22	37.863409267909496	40.0	38.0	40.0	34.0	40.0
23	37.91518730771754	40.0	38.0	40.0	34.0	40.0
24	37.89629286984254	40.0	37.0	40.0	34.0	40.0
25	37.83001195956959	40.0	37.0	40.0	34.0	40.0
26	37.66998585372992	40.0	37.0	40.0	34.0	40.0
27	37.53251034898058	40.0	37.0	40.0	33.0	40.0
28	37.32748463842908	40.0	36.0	40.0	33.0	40.0
29	37.52893828963016	40.0	37.0	40.0	33.0	40.0
30	37.53706608713497	40.0	37.0	40.0	33.0	40.0
31	37.504647074951684	40.0	37.0	40.0	33.0	40.0
32	36.76051727241074	40.0	35.0	40.0	31.0	40.0
33	36.92311426519294	40.0	35.0	40.0	31.0	40.0
34	36.83262355278274	40.0	35.0	40.0	31.0	40.0
35	36.874626368580145	40.0	35.0	40.0	32.0	40.0
36	36.64443442673964	39.0	35.0	40.0	31.0	40.0
37	36.52080544589346	39.0	35.0	40.0	31.0	40.0
38	36.306362120964906	39.0	35.0	40.0	30.0	40.0
39	36.22009308615768	39.0	35.0	40.0	30.0	40.0
40	36.052874417790996	39.0	35.0	40.0	30.0	40.0
41	35.83485600577289	38.0	35.0	40.0	30.0	40.0
42	35.727030813974025	38.0	35.0	40.0	29.0	40.0
43	35.59765888481639	38.0	35.0	40.0	29.0	40.0
44	35.42721029162174	37.0	35.0	40.0	29.0	40.0
45	35.269835476019466	37.0	35.0	40.0	28.0	40.0
46	35.00542604494847	37.0	34.0	40.0	27.0	40.0
47	34.862625756304006	36.0	34.0	40.0	27.0	40.0
48	34.695055533781996	36.0	34.0	40.0	27.0	40.0
49	34.35916858283306	35.0	34.0	40.0	26.0	40.0
50	33.95495464278565	35.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	12.0
14	14.0
15	28.0
16	83.0
17	209.0
18	492.0
19	983.0
20	1881.0
21	3097.0
22	4386.0
23	6372.0
24	9363.0
25	13120.0
26	17623.0
27	23774.0
28	31960.0
29	41343.0
30	51905.0
31	63955.0
32	76398.0
33	96977.0
34	170060.0
35	224097.0
36	270612.0
37	491208.0
38	894523.0
39	478035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.59636873152868	0.276567149366782	0.10721560698600344	0.019848512118525893
8	99.56047320198552	0.30062085473075834	0.05396103972561955	0.0849449035580981
9	98.58547391686837	0.6343113491437385	0.346171507965477	0.43404322602240863
10	64.29434334225394	22.585689222762543	5.05255650518164	8.067410929801868
11	35.14088238410908	27.223916447856446	17.61309867233639	20.02210249569809
12	30.687279963263432	16.71894002216978	26.81668553396703	25.77709448059976
13	23.045434589901145	17.76223164559304	30.491822581214894	28.700511183290917
14	25.606195427104655	17.813736852463318	31.28646953842117	25.293598182010857
15	25.461637704099054	17.080216584272915	28.39972212083034	29.058423590797695
16	29.71137235640527	17.19315125407273	26.797812626681445	26.297663762840557
17	32.14200769382156	17.431568890316782	23.147435757262787	27.278987658598865
18	30.786186108396425	17.74194579337699	24.203174752692586	27.268693345534
19	31.420867514545765	18.255416709419464	22.91275233262069	27.410963443414076
20	28.487358348065527	20.560434514207664	23.658215349628176	27.29399178809863
21	30.354060450493943	18.12138879030047	26.51007648405475	25.01447427515084
22	30.912611038127647	17.569162813307923	24.239944962430805	27.27828118613363
23	31.129363518771143	19.35771560446289	23.551840781291265	25.96108009547471
24	31.17753821259102	18.86860116769806	22.441804330676213	27.51205628903471
25	28.540377424504165	17.36828914235925	24.193250496633322	29.89808293650326
26	29.842237970203684	17.04243712815579	23.909046716332803	29.206278185307728
27	30.07779607504083	16.29219701162147	22.81795045609526	30.812056457242438
28	32.07475824344032	19.800438349343903	21.201070473992562	26.923732933223217
29	33.32134572912164	18.78940896849974	21.53354987275085	26.355695429627772
30	31.40899204882061	19.343249739698535	22.302932028938457	26.944826182542393
31	34.58246636265923	19.66173425533597	19.80740214935837	25.948397232646432
32	35.59268834640027	19.032570062724663	20.559963532564176	24.81477805831089
33	35.15857783728594	19.511995734251972	20.77156885667524	24.557857571786855
34	29.37213100690829	23.18252388970283	23.900737254479793	23.544607848909088
35	30.327584553820586	24.755232521955314	23.12035431276209	21.79682861146201
36	31.909679177396917	23.98194794643593	20.401612775713495	23.706760100453657
37	30.37400988725036	24.501911680849382	20.750441965810097	24.37363646609016
38	32.524343863697915	24.03294853011675	19.897191435535227	23.545516170650103
39	31.655483656095935	22.66905297366035	19.788899299078388	25.886564071165324
40	29.886207470778114	22.997529028448973	20.86401582498322	26.252247675789693
41	27.197137777269415	23.128630133069137	22.071343626525014	27.60288846313643
42	27.55084499153074	20.511486064830624	23.128058226787754	28.80961071685088
43	27.00847598750553	19.739580792695747	25.635329005909135	27.616614213889584
44	27.14421962546867	19.85658608955716	24.168591243442002	28.83060304153217
45	27.74805173396938	20.111790857237054	24.090374649076622	28.04978275971694
46	27.30115743738888	21.393903815455936	23.007823341513838	28.29711540564135
47	28.13876464879067	21.92463284457774	23.684960378669242	26.25164212796235
48	30.434261896071174	21.923253541193233	23.728425256054216	23.91405930668138
49	28.913193036872816	21.52624965727675	23.194567563157797	26.365989742692637
50	25.754016380068727	23.32277549482509	23.17529095732065	27.74791716778553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.5
4	11.0
5	37.0
6	63.0
7	141.0
8	219.0
9	2585.0
10	4951.0
11	6495.5
12	8040.0
13	6644.5
14	5249.0
15	4531.5
16	3814.0
17	4237.0
18	4660.0
19	4926.0
20	5192.0
21	4916.0
22	4640.0
23	5837.0
24	7034.0
25	8780.0
26	10526.0
27	12388.5
28	14251.0
29	14040.0
30	13829.0
31	15531.0
32	17233.0
33	21925.5
34	26618.0
35	31138.0
36	35658.0
37	37467.5
38	39277.0
39	42011.5
40	44746.0
41	44426.5
42	44107.0
43	49644.0
44	55181.0
45	61563.0
46	67945.0
47	75267.5
48	82590.0
49	99997.0
50	117404.0
51	136852.0
52	156300.0
53	183243.0
54	210186.0
55	261836.5
56	313487.0
57	329979.0
58	346471.0
59	332575.5
60	318680.0
61	318153.0
62	317626.0
63	271049.5
64	224473.0
65	188297.0
66	152121.0
67	129222.0
68	106323.0
69	89903.5
70	73484.0
71	63889.5
72	54295.0
73	54172.5
74	54050.0
75	37563.5
76	21077.0
77	13970.5
78	6864.0
79	4781.0
80	2698.0
81	1660.5
82	623.0
83	475.5
84	328.0
85	233.0
86	138.0
87	88.5
88	39.0
89	23.0
90	7.0
91	7.0
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2972515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.06181923895178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.97144062976295	5.479734677682918
2	10.594878818454816	1.708279957859635
3	4.574991537943141	1.1064826439589484
4	2.6470247386915964	0.8535933985746089
5	1.5745225056035241	0.6346757913918526
6	0.9995513796920626	0.483492152587336
7	0.6796892894766686	0.3835672533288731
8	0.47449125698619227	0.3060210195428559
9	0.39664973873104964	0.2877946645344456
>10	4.297985903510409	8.846034868478881
>50	2.893074257661414	16.934843730567085
>100	2.6843317898358694	38.70290710476493
>500	0.1347854892842269	7.420177801956538
>1k	0.06432943806747193	8.995806645137444
>5k	0.010065150173822139	5.431952301266827
>10k+	0.002188076124743943	2.4246359883668296
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	17640	0.5934368707979606	No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	15817	0.532108332506312	No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	12830	0.43162103471302926	No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	12349	0.415439451104536	No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	10090	0.3394431987727564	No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGA	9373	0.3153222103168529	No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	8903	0.2995106837139594	No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	8875	0.2985687204269785	No Hit
ATGCTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	7832	0.26348058798694035	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7327	0.2464916072753207	No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	7281	0.244944096160995	No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	7211	0.24258918794354276	No Hit
ATGCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	7082	0.23824942851423794	No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	7045	0.23700469131358462	No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	6715	0.22590298114559554	No Hit
ATGCTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	6441	0.2166851975515683	No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	6280	0.21126890865142814	No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	6234	0.2097213975371024	No Hit
ATGCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	6116	0.20575169511339725	No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	6049	0.2034977115338358	No Hit
ATGCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	6011	0.20221933278721888	No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	5898	0.19841783809333174	No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	5840	0.19646662842744278	No Hit
ATGCTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	5832	0.1961974960597339	No Hit
ATGCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	5727	0.1926651337335556	No Hit
ATGCTTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT	5507	0.18526399362156287	No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5353	0.18008319554316798	No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	5036	0.16941882547270576	No Hit
ATGCTTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4925	0.16568461387074582	No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3793	0.127602383839947	No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	3668	0.1233971905944966	No Hit
ATGCTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	3654	0.12292620895100613	No Hit
ATGCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	3560	0.11976390363042744	No Hit
ATGCTTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC	3542	0.11915835580308257	No Hit
ATGCTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	3472	0.11680344758563034	No Hit
ATGCTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3348	0.11263189588614354	No Hit
ATGCTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	3320	0.11168993259916267	No Hit
ATGCTTGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAA	3215	0.10815757027298432	No Hit
ATGCTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2987	0.1004872977932828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.0092463789080963E-4	0.0	0.0	0.0	0.0
8	5.382647354176514E-4	0.0	0.0	0.0	0.0
9	0.0010428879248716996	0.0	0.0	0.0	0.0
10	0.014331298580494967	0.0	0.0	0.0	0.0
11	0.02997461745357046	0.0	0.0	0.0	0.0
12	0.03925968413952495	0.0	0.0	0.0	0.0
13	0.04780463681428016	0.0	0.0	0.0	0.0
14	0.06348159723331925	0.0	0.0	0.0	0.0
15	0.07788017890574143	0.0	0.0	0.0	0.0
16	0.09439817797387061	0.0	0.0	0.0	0.0
17	0.10314497992440745	0.0	0.0	0.0	0.0
18	0.11269917897807076	0.0	0.0	0.0	0.0
19	0.12649221282314807	0.0	0.0	0.0	0.0
20	0.13581092105506617	0.0	0.0	0.0	0.0
21	0.14600430948203794	0.0	0.0	0.0	0.0
22	0.15636590563882774	0.0	0.0	0.0	0.0
23	0.1666938602496539	0.0	0.0	0.0	0.0
24	0.1778964950555338	0.0	0.0	0.0	0.0
25	0.18765254338497872	0.0	0.0	0.0	0.0
26	0.2002344815753663	0.0	0.0	0.0	0.0
27	0.21227815503033626	0.0	0.0	0.0	0.0
28	0.23703833285954823	0.0	0.0	0.0	0.0
29	0.2484764584871733	0.0	0.0	0.0	0.0
30	0.2609911135856337	0.0	0.0	0.0	0.0
31	0.2742458826952934	0.0	0.0	0.0	0.0
32	0.2900910508441505	0.0	0.0	0.0	0.0
33	0.3052970296196991	0.0	0.0	0.0	0.0
34	0.3228915581586636	0.0	0.0	0.0	0.0
35	0.36622186935978457	0.0	0.0	0.0	0.0
36	0.387045986311255	0.0	0.0	0.0	0.0
37	0.40312664528185727	0.0	0.0	0.0	0.0
38	0.4201156259934769	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	35	1.4471152E-7	44.0	18
CGTATTA	30	2.5290774E-6	44.0	34
CGTATAG	35	1.4471152E-7	44.0	40
ACGTTAA	30	2.5290774E-6	44.0	10
TACGGGT	40	8.3200575E-9	44.0	24
ACTTACG	35	1.4471152E-7	44.0	19
TTATCGA	20	7.8585E-4	44.0	38
TCGATAG	40	8.3200575E-9	44.0	17
ATATACG	20	7.8585E-4	44.0	29
GTCAATC	20	7.8585E-4	44.0	39
TTACGTA	50	2.7284841E-11	44.0	44
TATAGGG	35	1.4471152E-7	44.0	43
ACGTATC	20	7.8585E-4	44.0	43
ATAACGT	20	7.8585E-4	44.0	11
ACGTAAT	25	4.4445507E-5	44.0	21
ACGTAAC	50	2.7284841E-11	44.0	29
CGATCTA	50	2.7284841E-11	44.0	44
TATAACG	45	4.802132E-10	44.0	35
CGATAAG	20	7.8585E-4	44.0	28
TTGGGAT	14980	0.0	41.532707	5
>>END_MODULE