FastQCFastQC Report
Fri 17 Jun 2016
SRR1527230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189791
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC169280.5306930767564395No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG153300.480595750630684No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA126200.39563720632480304No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG123890.38839535254817636No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT92570.29020710134300337No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC83550.2619293865961751No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG79560.24942073007291074No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT79220.24835482951704357No Hit
ATGCTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA77130.2418026761000956No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG71730.22487366727161748No Hit
ATGCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG67710.21227096069930598No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC66110.20725495808346064No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT65890.2065652577237819No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC65100.20408860643220825No Hit
ATGCTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG62900.1971916028354209No Hit
ATGCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA60790.19057674938577482No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC58550.1835543457235913No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC58310.1828019453312145No Hit
ATGCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT56830.17816214291155752No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC55080.17267589005047665No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54870.17201753970714698No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG54360.17041868887334624No Hit
ATGCTTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC53970.16919603823573393No Hit
ATGCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC53490.16769123745098033No Hit
ATGCTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA52970.16606103660083057No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT50020.1568127817778657No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG49240.1543674805026411No Hit
ATGCTTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG46280.14508787566332715No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT41070.1287545171454807No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36250.11364380926524653No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC35780.11217035849684195No Hit
ATGCTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC34860.10928615699273088No Hit
ATGCTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT34760.10897265682924054No Hit
ATGCTTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG32880.10307885375562223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACC351.0203257E-746.00000446
TATAACG351.0203257E-746.00000435
TCGATAG253.41853E-546.017
CGAATAG253.41853E-546.027
ATACGTT206.3131494E-446.035
CGTTTAT253.41853E-546.028
ATACCGA253.41853E-546.016
ATACGCA750.042.93333414
TTGGGAC135850.042.596985
TTGGGAT163100.042.5450675
TGGGATT65900.042.440066
TTGGGGC473950.041.8799445
CTTGGGA769250.041.8469964
TTGGGGT313250.041.403675
ATGCTTG3530550.041.402671
TTGGGAG322550.041.372195
TGCTTGG3520550.041.343232
GCTTGGG3474050.041.3345223
TGGGGCT249800.041.239796
TGGGGAC142400.041.1383446
Produced by FastQC (version 0.11.5)