##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3189791
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43173675015071	33.0	31.0	34.0	31.0	34.0
2	32.79327767869431	34.0	31.0	34.0	31.0	34.0
3	32.969214910945574	34.0	31.0	34.0	31.0	34.0
4	36.328002994553565	37.0	37.0	37.0	35.0	37.0
5	36.283533623362786	37.0	37.0	37.0	35.0	37.0
6	36.48965684585605	37.0	37.0	37.0	35.0	37.0
7	36.52767281618137	37.0	37.0	37.0	35.0	37.0
8	36.608632665901936	37.0	37.0	37.0	35.0	37.0
9	38.72007758502046	39.0	39.0	39.0	39.0	39.0
10	38.177663991151775	39.0	39.0	39.0	37.0	39.0
11	37.86110500656626	39.0	38.0	39.0	35.0	39.0
12	37.42847854295156	39.0	37.0	39.0	35.0	39.0
13	37.3012780461165	39.0	37.0	39.0	35.0	39.0
14	38.13226038947379	40.0	38.0	40.0	35.0	40.0
15	38.058952765243866	40.0	38.0	40.0	34.0	40.0
16	32.976610693302476	40.0	35.0	40.0	3.0	40.0
17	34.7018475505135	40.0	34.0	40.0	17.0	40.0
18	36.79648980136943	40.0	35.0	40.0	31.0	40.0
19	37.61390856015331	40.0	37.0	40.0	33.0	40.0
20	37.876925792316804	40.0	37.0	40.0	34.0	40.0
21	38.088935607379916	40.0	38.0	40.0	34.0	40.0
22	38.14073649339408	40.0	38.0	40.0	34.0	40.0
23	38.09904974965445	40.0	38.0	40.0	34.0	40.0
24	38.039212913949534	40.0	38.0	40.0	34.0	40.0
25	37.98464068649012	40.0	37.0	40.0	34.0	40.0
26	37.866896922086745	40.0	37.0	40.0	34.0	40.0
27	37.730844121135206	40.0	37.0	40.0	34.0	40.0
28	37.59323165687031	40.0	37.0	40.0	33.0	40.0
29	37.39321573106201	40.0	36.0	40.0	33.0	40.0
30	37.33184274455599	40.0	36.0	40.0	33.0	40.0
31	37.29223011789801	40.0	36.0	40.0	33.0	40.0
32	37.173078424260396	40.0	35.0	40.0	33.0	40.0
33	36.78975487735717	39.0	35.0	40.0	31.0	40.0
34	36.63431177779359	39.0	35.0	40.0	31.0	40.0
35	36.60776458394923	39.0	35.0	40.0	31.0	40.0
36	36.515401792782036	39.0	35.0	40.0	31.0	40.0
37	36.14895145167818	38.0	35.0	40.0	30.0	40.0
38	36.13611832248571	38.0	35.0	40.0	30.0	40.0
39	35.727130398198504	38.0	35.0	40.0	29.0	40.0
40	35.61979546622334	38.0	35.0	40.0	29.0	40.0
41	35.475024225725136	38.0	34.0	40.0	29.0	40.0
42	35.427368125372475	38.0	34.0	40.0	29.0	40.0
43	35.30092096943029	37.0	34.0	40.0	29.0	40.0
44	35.544347262877096	37.0	35.0	40.0	30.0	40.0
45	35.56862158053615	37.0	35.0	40.0	30.0	40.0
46	35.518173447727456	37.0	35.0	40.0	30.0	40.0
47	35.16999953915476	37.0	35.0	40.0	29.0	40.0
48	35.07559931042504	36.0	34.0	40.0	29.0	40.0
49	34.956093988603016	36.0	34.0	40.0	29.0	40.0
50	34.439711880809746	35.0	34.0	39.0	27.0	40.0
51	34.44148190273281	35.0	34.0	39.0	27.0	40.0
52	34.09336160268808	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	5.0
14	4.0
15	35.0
16	75.0
17	211.0
18	431.0
19	872.0
20	1665.0
21	2768.0
22	4573.0
23	7080.0
24	10465.0
25	14931.0
26	20267.0
27	27952.0
28	36762.0
29	45252.0
30	54906.0
31	69039.0
32	91857.0
33	137017.0
34	202121.0
35	274711.0
36	392334.0
37	640481.0
38	792847.0
39	361126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.6220128528797	0.26616163880329463	0.09514729961931676	0.016678208697685836
8	99.55429681756578	0.3065091098445008	0.06313893292695352	0.07605513966275533
9	98.6100970251656	0.63772203257204	0.3290497715994559	0.4231311706629055
10	64.41255869114936	22.52978329928199	4.959447186351708	8.098210823216945
11	35.21302806359414	27.26369846801875	17.49964182606321	20.023631642323902
12	30.869357898370144	16.77417109773023	26.741125045496712	25.61534595840292
13	23.271587386132822	17.714452138086788	30.397258002170048	28.61670247361034
14	25.80153370549983	17.787811176343528	31.12956303406712	25.281092084089522
15	25.559354829203546	17.23595683855149	28.290066653269758	28.914621678975205
16	28.115447062205646	25.56963763456603	23.63951117800508	22.67540412522325
17	32.230732358326925	17.35289239953339	23.086590939657174	27.329784302482512
18	30.935882633062796	17.663571061552307	24.14104873955692	27.259497565827978
19	31.522692239084005	18.216836150080052	22.84469421350803	27.41577739732791
20	28.468542296344808	20.537333010219164	23.652866284969768	27.341258408466263
21	30.436884422835224	18.09272143535423	26.490324914704445	24.980069227106103
22	30.710476015513244	17.56390935957873	24.323725284822736	27.40188934008529
23	31.158122898961093	19.37202155250924	23.499752805121087	25.970102743408578
24	31.266938805708588	18.860640085823803	22.37666981943331	27.4957512890343
25	28.53697938203475	17.320445132612136	24.218420579906333	29.92415490544678
26	29.98077303497314	16.944652486636272	23.869338147859846	29.20523633053075
27	30.18674264238629	16.277555488745186	22.678131576645615	30.857570292222906
28	32.098278539252256	19.775590312970348	21.232268822628193	26.893862325149204
29	33.537463739787334	18.787531847697856	21.356289487304966	26.318714925209836
30	31.511061383018514	19.361143096836127	22.149194100804724	26.97860141934064
31	34.759424677039966	19.51466412689734	19.764022156937557	25.961889039125136
32	35.837426339217835	18.45149102245257	20.626178956552327	25.08490368177727
33	35.340716680183746	19.370548101740834	20.80672370070641	24.482011517369006
34	29.450706958543677	23.028562059395114	23.877677252208688	23.64305372985252
35	30.42017486412119	24.701587031877636	23.063580027656986	21.814658076344188
36	31.89190138162657	23.857080291467373	20.538336210742333	23.712682116163723
37	30.63166207441177	24.47668201458967	20.610284498263365	24.281371412735194
38	32.466233681140864	24.054083794204697	19.868198261265395	23.611484263389045
39	31.94522775943628	22.600132735969222	19.72912958874108	25.725509915853422
40	29.95183696988298	22.959466623361845	20.831270763507703	26.257425643247473
41	27.27253917262918	23.092860942926983	22.094801822439152	27.53979806200469
42	27.447723063987578	20.50889854539059	23.13151551308534	28.911862877536493
43	27.096414780780307	19.708501277983416	25.548162873366937	27.646921067869336
44	27.14456840589242	19.811924981918878	24.185220912592705	28.858285699595992
45	27.78247853856256	20.07429326874394	24.04662249031363	28.096605702379872
46	26.981987221106333	21.408017014280873	23.12731461089457	28.48268115371822
47	28.430075826284547	21.794343265750012	23.67653554731329	26.099045360652156
48	30.588775252046297	21.877452159091302	23.686943752741165	23.84682883612124
49	29.052812551041747	21.278666846824763	23.252369826110865	26.41615077602263
50	25.770058289085394	23.344570224193372	23.002949096037952	27.882422390683274
51	26.409222422409496	24.374982561553406	23.04749746926993	26.16829754676717
52	28.958825202027345	22.603675287816664	21.965577055048435	26.47192245510756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	3.0
4	6.0
5	34.5
6	63.0
7	167.5
8	272.0
9	2562.5
10	4853.0
11	6511.5
12	8170.0
13	6857.0
14	4683.5
15	3823.0
16	4138.0
17	4453.0
18	4832.5
19	5212.0
20	4643.5
21	4075.0
22	4952.0
23	5829.0
24	7596.0
25	9363.0
26	11532.0
27	13701.0
28	13473.5
29	13246.0
30	15274.5
31	17303.0
32	21333.0
33	25363.0
34	29549.5
35	33736.0
36	36334.0
37	38932.0
38	38689.5
39	39784.5
40	41122.0
41	44734.0
42	48346.0
43	51964.5
44	55583.0
45	63378.5
46	71174.0
47	77694.0
48	84214.0
49	100796.5
50	117379.0
51	143358.0
52	169337.0
53	193488.5
54	217640.0
55	264661.5
56	311683.0
57	341172.5
58	370662.0
59	354503.5
60	338345.0
61	329717.0
62	321089.0
63	287171.5
64	217099.0
65	180944.0
66	151581.0
67	122218.0
68	104056.0
69	85894.0
70	75608.5
71	65323.0
72	62327.5
73	59332.0
74	43904.0
75	28476.0
76	19045.0
77	9614.0
78	6678.5
79	3743.0
80	2651.5
81	1560.0
82	947.0
83	334.0
84	213.0
85	92.0
86	61.0
87	30.0
88	19.5
89	5.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3189791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.413853842727384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.77312573743124	6.662478616851455
2	9.940432580199223	1.8715555888696187
3	4.367205895115624	1.2333671399314774
4	2.3939995068960678	0.9014704582992404
5	1.3525031930137617	0.636613369042683
6	0.8811149536149525	0.49768124351836096
7	0.6130044061041722	0.4039513718808806
8	0.4290813695312411	0.3231447439523522
9	0.3456944132816796	0.29288850127930155
>10	3.932122925613952	9.5422488882334
>50	2.600779286573212	17.818611762567237
>100	2.2024095590469375	36.9822462211243
>500	0.1033524359699834	6.566360394535277
>1k	0.055147008745177716	9.030633345686699
>5k	0.008484155191565803	5.157732471648576
>10k+	0.0015425736711937822	2.0790158825791263
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	16928	0.5306930767564395	No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	15330	0.480595750630684	No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	12620	0.39563720632480304	No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	12389	0.38839535254817636	No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9257	0.29020710134300337	No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	8355	0.2619293865961751	No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG	7956	0.24942073007291074	No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	7922	0.24835482951704357	No Hit
ATGCTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	7713	0.2418026761000956	No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	7173	0.22487366727161748	No Hit
ATGCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	6771	0.21227096069930598	No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6611	0.20725495808346064	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6589	0.2065652577237819	No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	6510	0.20408860643220825	No Hit
ATGCTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	6290	0.1971916028354209	No Hit
ATGCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	6079	0.19057674938577482	No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5855	0.1835543457235913	No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5831	0.1828019453312145	No Hit
ATGCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	5683	0.17816214291155752	No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	5508	0.17267589005047665	No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5487	0.17201753970714698	No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	5436	0.17041868887334624	No Hit
ATGCTTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	5397	0.16919603823573393	No Hit
ATGCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	5349	0.16769123745098033	No Hit
ATGCTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	5297	0.16606103660083057	No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	5002	0.1568127817778657	No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	4924	0.1543674805026411	No Hit
ATGCTTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	4628	0.14508787566332715	No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	4107	0.1287545171454807	No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3625	0.11364380926524653	No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3578	0.11217035849684195	No Hit
ATGCTTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3486	0.10928615699273088	No Hit
ATGCTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	3476	0.10897265682924054	No Hit
ATGCTTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG	3288	0.10307885375562223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.194501144432347E-4	0.0	0.0	0.0	0.0
8	3.4485017983936877E-4	0.0	0.0	0.0	0.0
9	9.718505068200393E-4	0.0	0.0	0.0	0.0
10	0.012947556752150846	0.0	0.0	0.0	0.0
11	0.027117764141914	0.0	0.0	0.0	0.0
12	0.034892568196474315	0.0	0.0	0.0	0.0
13	0.04241657212024236	0.0	0.0	0.0	0.0
14	0.05790348019666492	0.0	0.0	0.0	0.0
15	0.0695656862785054	0.0	0.0	0.0	0.0
16	0.08862649621871778	0.0	0.0	0.0	0.0
17	0.09784340102533363	0.0	0.0	0.0	0.0
18	0.10731110596274177	0.0	0.0	0.0	0.0
19	0.12151266336885394	0.0	0.0	0.0	0.0
20	0.1316700686659408	0.0	0.0	0.0	0.0
21	0.14267392440445156	0.0	0.0	0.0	0.0
22	0.15339562999582104	0.0	0.0	0.0	0.0
23	0.16364708534195502	0.0	0.0	0.0	0.0
24	0.17371044058999477	0.0	0.0	0.0	0.0
25	0.18292734539661062	0.0	0.0	0.0	0.0
26	0.19393120113512138	0.0	0.0	0.0	0.0
27	0.20499775690633024	0.0	0.0	0.0	0.0
28	0.22957616972397252	0.0	0.0	0.0	0.0
29	0.23942007485756903	0.0	0.0	0.0	0.0
30	0.25239898162606894	0.0	0.0	0.0	0.0
31	0.2652838383455217	0.0	0.0	0.0	0.0
32	0.2808961464873404	0.0	0.0	0.0	0.0
33	0.29490960379535835	0.0	0.0	0.0	0.0
34	0.3110235121987616	0.0	0.0	0.0	0.0
35	0.35422383472772984	0.0	0.0	0.0	0.0
36	0.3759807460739591	0.0	0.0	0.0	0.0
37	0.39234545460815456	0.0	0.0	0.0	0.0
38	0.4091177133548875	0.0	0.0	0.0	0.0
39	0.4267050725266953	0.0	0.0	0.0	0.0
40	0.4418784804396276	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACC	35	1.0203257E-7	46.000004	46
TATAACG	35	1.0203257E-7	46.000004	35
TCGATAG	25	3.41853E-5	46.0	17
CGAATAG	25	3.41853E-5	46.0	27
ATACGTT	20	6.3131494E-4	46.0	35
CGTTTAT	25	3.41853E-5	46.0	28
ATACCGA	25	3.41853E-5	46.0	16
ATACGCA	75	0.0	42.933334	14
TTGGGAC	13585	0.0	42.59698	5
TTGGGAT	16310	0.0	42.545067	5
TGGGATT	6590	0.0	42.44006	6
TTGGGGC	47395	0.0	41.879944	5
CTTGGGA	76925	0.0	41.846996	4
TTGGGGT	31325	0.0	41.40367	5
ATGCTTG	353055	0.0	41.40267	1
TTGGGAG	32255	0.0	41.37219	5
TGCTTGG	352055	0.0	41.34323	2
GCTTGGG	347405	0.0	41.334522	3
TGGGGCT	24980	0.0	41.23979	6
TGGGGAC	14240	0.0	41.138344	6
>>END_MODULE