FastQCFastQC Report
Fri 17 Jun 2016
SRR1527229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107788
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC158640.5104595294144902No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG145430.4679534125236342No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA124830.40166832486643234No Hit
ATGCTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG123080.396037310138272No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT99650.32064606723495936No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC82780.2663630852554936No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG79890.25706386664727454No Hit
ATGCTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT79420.2555515369774258No Hit
ATGCTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA73490.23647044135571668No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC69400.22330995550533048No Hit
ATGCTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG67870.21838683977156745No Hit
ATGCTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG67500.21719628237189922No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT67370.21677797842066449No Hit
ATGCTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG62690.20171903617621278No Hit
ATGCTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC62290.2004319470954904No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC58450.1880758919205557No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC57460.18489034644576785No Hit
ATGCTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA55960.180063762393059No Hit
ATGCTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT55150.1774574070045962No Hit
ATGCTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA54370.17494758329718757No Hit
ATGCTTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG54320.1747866971620973No Hit
ATGCTTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC53620.17253429127083314No Hit
ATGCTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC52120.16770770721812428No Hit
ATGCTTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC51390.16535876964580595No Hit
ATGCTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT49220.15837631138288713No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49150.1581510707937607No Hit
ATGCTTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG47760.15367843623825048No Hit
ATGCTTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG44920.1445401037651217No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT39780.1280010090778393No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34120.10978869858561781No Hit
ATGCTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT33730.1085337867319135No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC33640.10824419168875096No Hit
ATGCTTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG33590.10808330555366068No Hit
ATGCTTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG31530.10145479678794049No Hit
ATGCTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT31310.10074689779354319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACTA351.0203257E-746.00000442
TATCGAA301.862436E-646.039
TTGGGAT160750.042.880875
TGGGATT62100.042.6666686
TTGGGGC462900.042.2635575
TTGGGAC136100.042.180755
TGGGGCT244050.041.8156136
CTTGGGA760000.041.7903944
ATGCTTG3423550.041.578771
TGGGATA21150.041.541376
TTGGGGT302950.041.5131235
TGCTTGG3417500.041.4895132
GCTTGGG3373200.041.4739153
CCGAATA508.731149E-1041.434
TTGGGAG318500.041.2555735
TGGGGAC136150.041.253036
TTGGGTG78350.041.2150575
TGGGGGC211600.041.1195646
CTTGGGG2200900.041.108234
TGGGGTC84650.040.973426
Produced by FastQC (version 0.11.5)