FastQCFastQC Report
Fri 17 Jun 2016
SRR1527228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577046
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC104960.6655481197124243No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT94940.6020116090462801No Hit
GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG83950.5323243583256291No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC59600.377921760050119No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC58640.3718344296868956No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG54630.3464071434821812No Hit
GTCGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC47850.303415372791916No Hit
GTCGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC39110.24799530261006972No Hit
GTCGGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA37540.23803998107854812No Hit
GTCGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC37110.23531336435335432No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC36920.23410858021896633No Hit
GTCGGTGGGCTGTAGGTGTCTGTGTGGGTGTGGGTGTGCTATAGGTATCT36750.2330306154671455No Hit
GTCGGTGGGTCATTCAGATTGCCATCATCTCTATCTTCAAGTCCTATCCA34690.21996821906272868No Hit
GTCGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA31460.1994868887781333No Hit
GTCGGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG29990.19016566415944747No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC29980.1901022544681639No Hit
GTCGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG29190.1850928888567613No Hit
GTCGGTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT28110.178244642198135No Hit
GTCGGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT27340.17336209596929955No Hit
GTCGGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT27190.1724109506000459No Hit
GTCGGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT24820.15738285376583816No Hit
GTCGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG23080.14634956748249575No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT22980.14571547056966No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG22630.1434961313747348No Hit
GTCGGTGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC22500.1426718053880483No Hit
GTCGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT22430.14222793754906327No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC21100.13379444860834752No Hit
GTCGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT20890.1324628450913924No Hit
GTCGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC19740.12517073059378103No Hit
GTCGGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC19680.12479027244607958No Hit
GTCGGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA19470.12345866892912445No Hit
GTCGGTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT19460.12339525923784087No Hit
GTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA18880.1197174971433934No Hit
GTCGGTGGGATCTTACAGTTACCTCATTTTTCCCATGTATGTATTTGAGA18540.1175615676397518No Hit
GTCGGTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC18190.1153422284448266No Hit
GTCGGTGGGGTTCCCCTGTTTTGAGTATTTCTGTCCCTGGAATAACCTTT18140.11502517998840871No Hit
GTCGGTGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG17620.1117278760416627No Hit
GTCGGTGGGCGATGGCAAGAACGTCAATGCGGGTGGCCACAAGCTTGGCC17180.10893784962518532No Hit
GTCGGTGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG17140.108684210860051No Hit
GTCGGTGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCT17030.10798670425593165No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT16670.1057039553697229No Hit
GTCGGTGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC16530.10481621969175281No Hit
GTCGGTGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA16360.103738254939932No Hit
GTCGGTGGGGACTGTGGCCGCGGCGCGGGAAGGACAGTGAGCGGCCGCGA16110.10215301265784257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT207.8573753E-444.034
CGTATGA302.5283189E-644.018
GATAACG302.5283189E-644.019
CGAAATT454.802132E-1044.021
TTTACGC207.8573753E-444.034
CAACGAA302.5283189E-644.020
TAGCGTA207.8573753E-444.024
CTATACT302.5283189E-644.012
CTATACG302.5283189E-644.020
ATTACGT351.4465513E-744.031
CGACCTA207.8573753E-444.044
GCACGTA207.8573753E-444.029
CAACCCG351.4465513E-744.018
TGACGTA302.5283189E-644.040
TAACGGG207.8573753E-444.014
CGCGAAT351.4465513E-744.042
ATCACGA254.443596E-544.021
TTATCGA254.443596E-544.042
TAGTTAG207.8573753E-444.030
CGTTACG302.5283189E-644.044
Produced by FastQC (version 0.11.5)