##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1577046
Sequences flagged as poor quality	0
Sequence length	50
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28074894454569	31.0	31.0	31.0	31.0	33.0
2	31.41836382705387	31.0	31.0	33.0	30.0	34.0
3	32.33198207281208	33.0	31.0	34.0	31.0	34.0
4	36.30309769023859	37.0	35.0	37.0	35.0	37.0
5	36.30438680926238	37.0	35.0	37.0	35.0	37.0
6	33.16757976622115	35.0	32.0	35.0	30.0	35.0
7	35.370061494718605	35.0	35.0	37.0	35.0	37.0
8	36.33406951984914	37.0	35.0	37.0	35.0	37.0
9	38.657995391383636	39.0	39.0	39.0	37.0	39.0
10	37.95275851179991	39.0	38.0	39.0	35.0	39.0
11	37.056195570706244	39.0	37.0	39.0	34.0	39.0
12	36.32559608280291	37.0	35.0	39.0	32.0	39.0
13	36.12748772071328	37.0	35.0	39.0	32.0	39.0
14	36.89703027051843	38.0	36.0	40.0	32.0	40.0
15	36.85707772633138	38.0	36.0	40.0	32.0	40.0
16	36.64638063823122	38.0	35.0	40.0	32.0	40.0
17	36.475452840310304	38.0	35.0	40.0	31.0	40.0
18	36.35791156377176	38.0	35.0	40.0	31.0	40.0
19	36.302180786102625	38.0	35.0	40.0	30.0	40.0
20	35.958424167716096	38.0	34.0	40.0	30.0	40.0
21	36.17715272731423	38.0	35.0	40.0	30.0	40.0
22	35.99683078362965	38.0	34.0	40.0	30.0	40.0
23	35.91158976973405	38.0	34.0	39.0	30.0	40.0
24	35.96345953130093	38.0	34.0	39.0	30.0	40.0
25	35.83025923149991	38.0	34.0	39.0	30.0	40.0
26	35.549489361756095	38.0	34.0	39.0	30.0	40.0
27	35.29546189521422	38.0	34.0	39.0	29.0	40.0
28	34.968529136119045	37.0	33.0	39.0	27.0	40.0
29	35.64909203663051	38.0	34.0	40.0	30.0	40.0
30	35.97350426049716	38.0	34.0	40.0	30.0	40.0
31	35.96950247488025	38.0	34.0	40.0	30.0	40.0
32	35.36044668322928	38.0	34.0	40.0	28.0	40.0
33	35.47264696147101	38.0	34.0	40.0	28.0	40.0
34	35.215119279970274	38.0	33.0	40.0	27.0	40.0
35	35.239994267763905	38.0	33.0	40.0	27.0	40.0
36	35.14204785402582	38.0	33.0	40.0	27.0	40.0
37	34.98916835653494	37.0	33.0	40.0	27.0	40.0
38	34.81052106279715	37.0	33.0	40.0	26.0	40.0
39	34.692589182560305	37.0	33.0	40.0	26.0	40.0
40	34.46461612406994	36.0	33.0	40.0	26.0	40.0
41	34.2412339272285	36.0	33.0	39.0	26.0	40.0
42	34.144028138684604	36.0	33.0	39.0	26.0	40.0
43	34.015283003793165	35.0	33.0	39.0	25.0	40.0
44	33.89150855460145	35.0	32.0	39.0	25.0	40.0
45	33.74028595234381	35.0	32.0	39.0	24.0	40.0
46	33.39323710278584	35.0	32.0	39.0	23.0	40.0
47	33.255357167768096	35.0	32.0	39.0	23.0	40.0
48	32.99043718445753	35.0	31.0	38.0	23.0	40.0
49	32.64967794217797	35.0	31.0	38.0	22.0	40.0
50	32.08963023272625	34.0	30.0	37.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	7.0
15	28.0
16	99.0
17	205.0
18	383.0
19	783.0
20	1427.0
21	2261.0
22	3548.0
23	5393.0
24	8094.0
25	11304.0
26	15033.0
27	20928.0
28	27231.0
29	35667.0
30	46996.0
31	61697.0
32	83272.0
33	114175.0
34	153121.0
35	172145.0
36	242841.0
37	299944.0
38	263324.0
39	7137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.33946124589897	0.6221124811831741	0.0314512068766542	0.0069750660411934725
8	99.62461462760123	0.321106676660034	0.028597770768893232	0.025680924969848692
9	98.66338711743347	0.9198843914508518	0.15776331191353962	0.2589651792021285
10	66.01779529576183	22.01400593261072	4.223148849177513	7.745049922449948
11	33.37385212606354	25.54136023933354	19.787945310409462	21.29684232419346
12	29.23567226320602	16.962092418356853	27.966844340621645	25.835390977815486
13	22.214126918301684	15.549768364397742	32.25847565638542	29.977629060915156
14	23.45315228598278	16.310050562887827	33.165868338653404	27.070928812475987
15	24.92609600480899	16.592857786012583	29.775605784485677	28.705440424692746
16	30.50209061752162	17.01878068236437	25.78941895163489	26.689709748479117
17	31.477078030697896	17.03850109635356	23.935065939737964	27.549354933210573
18	29.63096827866784	19.166910793978108	23.784024055100485	27.418096872253567
19	30.000456549777244	18.96685321797842	22.801617708044027	28.231072524200307
20	30.394928239252373	19.225184300267717	22.777331796282414	27.602555664197492
21	31.81162756191005	17.93169000777403	25.47826759650638	24.77841483380954
22	32.65763966301554	16.808640965450596	24.321865056567784	26.211854314966082
23	31.754305200989698	18.806046240883273	22.624133982141295	26.815514575985734
24	32.30818885435174	18.34562847247322	22.473662784725366	26.87251988844967
25	30.120110637229352	17.913428016684357	23.15867767966185	28.80778366642444
26	28.086561837765032	18.523556066214937	23.84350234552448	29.54637975049555
27	30.000710188542378	17.090116585058393	23.707044689882224	29.20212853651701
28	30.632460942800655	19.30520733066759	21.86175926383885	28.200572462692907
29	31.22198084266407	19.409769911594207	21.732340083929067	27.635909161812656
30	29.937871184480354	18.51975148473792	24.121046564272696	27.421330766509026
31	33.39534801140867	19.256698916835653	19.689660288919917	27.65829278283576
32	34.69220301754039	20.202200823565068	20.30891933399533	24.79667682489921
33	34.98509238157923	18.666861968515818	21.237871311299735	25.110174338605212
34	30.905820121924155	20.853925630577674	25.380997130077372	22.859257117420796
35	29.7809321985535	22.786843249974954	24.228906449146063	23.203318102325486
36	31.871993588012014	22.76211347037436	20.26053773954596	25.105355202067663
37	31.488111316981243	23.032175345551114	20.246270559007158	25.233442778460493
38	32.67203366293691	22.228774556988192	20.244495087651217	24.854696692423683
39	32.56182761948605	22.250460671407176	19.50482103882829	25.682890670278486
40	30.34305911178241	21.114793100518312	21.59448741507857	26.947660372620707
41	28.93523714590443	21.85155030354219	22.23245231908264	26.980760231470736
42	26.949499253667934	19.78775508133561	24.11502264360076	29.1477230213957
43	26.61970544930205	18.115134244657416	25.910404642603957	29.354755663436578
44	26.940621896888235	18.94998624009699	24.44411894136252	29.665272921652253
45	29.08830814066299	19.045608054552627	24.761167397780408	27.10491640700398
46	28.87721727837996	20.846696925771347	23.07764009420144	27.198445701647255
47	26.878416989739044	20.536813764468505	25.183539351420315	27.401229894372136
48	30.052008628790787	19.510844959500233	25.22139493711661	25.21575147459237
49	28.53791202032154	20.049129828806517	24.701879336430263	26.711078814441684
50	26.12403189253833	21.093487444247028	24.7553971158736	28.027083547341043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	3.5
12	6.0
13	403.5
14	801.0
15	965.0
16	1129.0
17	1335.5
18	1542.0
19	1453.0
20	1364.0
21	1376.5
22	1389.0
23	1954.5
24	2520.0
25	2894.0
26	3268.0
27	3281.5
28	3295.0
29	4427.0
30	5559.0
31	5977.5
32	6396.0
33	7714.5
34	9033.0
35	12480.0
36	15927.0
37	15937.5
38	15948.0
39	19097.0
40	22246.0
41	21938.0
42	21630.0
43	24443.0
44	27256.0
45	31427.0
46	35598.0
47	35181.5
48	34765.0
49	37480.0
50	40195.0
51	45045.0
52	49895.0
53	55022.5
54	60150.0
55	66602.0
56	73054.0
57	81759.0
58	90464.0
59	114171.5
60	137879.0
61	145912.5
62	153946.0
63	157647.5
64	161349.0
65	166860.0
66	172371.0
67	153180.5
68	133990.0
69	113964.0
70	93938.0
71	78719.0
72	63500.0
73	56956.0
74	50412.0
75	43267.0
76	36122.0
77	34141.5
78	32161.0
79	22407.0
80	12653.0
81	7757.0
82	2861.0
83	1978.5
84	1096.0
85	918.0
86	740.0
87	576.0
88	412.0
89	260.0
90	108.0
91	86.0
92	64.0
93	36.5
94	9.0
95	6.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1577046.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.261563364382972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.26927686099728	5.640109566277333
2	10.83628926422309	1.7904938078232402
3	4.710256450414035	1.1674224638276756
4	2.555927871732069	0.8446384026842801
5	1.46552942750627	0.6053782113855478
6	0.88191653791895	0.4371605616068799
7	0.6147145740001583	0.3554952382878396
8	0.4351748887069857	0.28761799341128563
9	0.3581341083260697	0.26628728659943585
>10	4.003745428245852	8.603808725921365
>50	2.7665696799276813	16.781062455644786
>100	2.9031511911066596	43.192624166266974
>500	0.13022888275204927	7.305715011906029
>1k	0.06432036282265849	9.725952060623161
>5k	0.003970392766830771	2.307661781027698
>10k+	7.940785533661543E-4	0.688572266706468
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10496	0.6655481197124243	No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	9494	0.6020116090462801	No Hit
GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	8395	0.5323243583256291	No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5960	0.377921760050119	No Hit
GTCGGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5864	0.3718344296868956	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5463	0.3464071434821812	No Hit
GTCGGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	4785	0.303415372791916	No Hit
GTCGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	3911	0.24799530261006972	No Hit
GTCGGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	3754	0.23803998107854812	No Hit
GTCGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	3711	0.23531336435335432	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	3692	0.23410858021896633	No Hit
GTCGGTGGGCTGTAGGTGTCTGTGTGGGTGTGGGTGTGCTATAGGTATCT	3675	0.2330306154671455	No Hit
GTCGGTGGGTCATTCAGATTGCCATCATCTCTATCTTCAAGTCCTATCCA	3469	0.21996821906272868	No Hit
GTCGGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3146	0.1994868887781333	No Hit
GTCGGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	2999	0.19016566415944747	No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2998	0.1901022544681639	No Hit
GTCGGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2919	0.1850928888567613	No Hit
GTCGGTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT	2811	0.178244642198135	No Hit
GTCGGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	2734	0.17336209596929955	No Hit
GTCGGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	2719	0.1724109506000459	No Hit
GTCGGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT	2482	0.15738285376583816	No Hit
GTCGGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2308	0.14634956748249575	No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2298	0.14571547056966	No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2263	0.1434961313747348	No Hit
GTCGGTGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC	2250	0.1426718053880483	No Hit
GTCGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	2243	0.14222793754906327	No Hit
GTCGGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2110	0.13379444860834752	No Hit
GTCGGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	2089	0.1324628450913924	No Hit
GTCGGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1974	0.12517073059378103	No Hit
GTCGGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	1968	0.12479027244607958	No Hit
GTCGGTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	1947	0.12345866892912445	No Hit
GTCGGTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	1946	0.12339525923784087	No Hit
GTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	1888	0.1197174971433934	No Hit
GTCGGTGGGATCTTACAGTTACCTCATTTTTCCCATGTATGTATTTGAGA	1854	0.1175615676397518	No Hit
GTCGGTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC	1819	0.1153422284448266	No Hit
GTCGGTGGGGTTCCCCTGTTTTGAGTATTTCTGTCCCTGGAATAACCTTT	1814	0.11502517998840871	No Hit
GTCGGTGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	1762	0.1117278760416627	No Hit
GTCGGTGGGCGATGGCAAGAACGTCAATGCGGGTGGCCACAAGCTTGGCC	1718	0.10893784962518532	No Hit
GTCGGTGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG	1714	0.108684210860051	No Hit
GTCGGTGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCT	1703	0.10798670425593165	No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1667	0.1057039553697229	No Hit
GTCGGTGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	1653	0.10481621969175281	No Hit
GTCGGTGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA	1636	0.103738254939932	No Hit
GTCGGTGGGGACTGTGGCCGCGGCGCGGGAAGGACAGTGAGCGGCCGCGA	1611	0.10215301265784257	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.340969128357701E-5	0.0	0.0	0.0	0.0
8	3.170484564178851E-4	0.0	0.0	0.0	0.0
9	5.072775302686161E-4	0.0	0.0	0.0	0.0
10	0.002409568268775927	0.0	0.0	0.0	0.0
11	0.005453233450387623	0.0	0.0	0.0	0.0
12	0.008433488940715743	0.0	0.0	0.0	0.0
13	0.010779647518208093	0.0	0.0	0.0	0.0
14	0.014076951464954099	0.0	0.0	0.0	0.0
15	0.018959497693789528	0.0	0.0	0.0	0.0
16	0.02422250207032642	0.0	0.0	0.0	0.0
17	0.027329576943221693	0.0	0.0	0.0	0.0
18	0.0306268808899677	0.0	0.0	0.0	0.0
19	0.03512896897110167	0.0	0.0	0.0	0.0
20	0.03810922446142979	0.0	0.0	0.0	0.0
21	0.04153334779074295	0.0	0.0	0.0	0.0
22	0.044069735442086026	0.0	0.0	0.0	0.0
23	0.0480011363016678	0.0	0.0	0.0	0.0
24	0.051361849939697383	0.0	0.0	0.0	0.0
25	0.05554688956441347	0.0	0.0	0.0	0.0
26	0.05985874857169671	0.0	0.0	0.0	0.0
27	0.06309264282715914	0.0	0.0	0.0	0.0
28	0.06778495998214383	0.0	0.0	0.0	0.0
29	0.07120908331145699	0.0	0.0	0.0	0.0
30	0.07653549737927746	0.0	0.0	0.0	0.0
31	0.0824960083599337	0.0	0.0	0.0	0.0
32	0.09130995544835091	0.0	0.0	0.0	0.0
33	0.09714364704643999	0.0	0.0	0.0	0.0
34	0.10335779679223053	0.0	0.0	0.0	0.0
35	0.11141082758524481	0.0	0.0	0.0	0.0
36	0.11768838702231894	0.0	0.0	0.0	0.0
37	0.12402935615067665	0.0	0.0	0.0	0.0
38	0.1291021314533628	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	20	7.8573753E-4	44.0	34
CGTATGA	30	2.5283189E-6	44.0	18
GATAACG	30	2.5283189E-6	44.0	19
CGAAATT	45	4.802132E-10	44.0	21
TTTACGC	20	7.8573753E-4	44.0	34
CAACGAA	30	2.5283189E-6	44.0	20
TAGCGTA	20	7.8573753E-4	44.0	24
CTATACT	30	2.5283189E-6	44.0	12
CTATACG	30	2.5283189E-6	44.0	20
ATTACGT	35	1.4465513E-7	44.0	31
CGACCTA	20	7.8573753E-4	44.0	44
GCACGTA	20	7.8573753E-4	44.0	29
CAACCCG	35	1.4465513E-7	44.0	18
TGACGTA	30	2.5283189E-6	44.0	40
TAACGGG	20	7.8573753E-4	44.0	14
CGCGAAT	35	1.4465513E-7	44.0	42
ATCACGA	25	4.443596E-5	44.0	21
TTATCGA	25	4.443596E-5	44.0	42
TAGTTAG	20	7.8573753E-4	44.0	30
CGTTACG	30	2.5283189E-6	44.0	44
>>END_MODULE